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Bernardinelli 2017 PLoS One

From Bioblast
Publications in the MiPMap
Bernardinelli E, Costa R, Scantamburlo G, To J, Morabito R, Nofziger C, Doerrier C, Krumschnabel G, Paulmichl M, Dossena S (2017) Mis-targeting of the mitochondrial protein LIPT2 leads to apoptotic cell death. PLoS One 12(6):e0179591.

Β» PMID: 28628643 Open Access

Bernardinelli E, Costa R, Scantamburlo G, To J, Morabito R, Nofziger C, Doerrier C, Krumschnabel G, Paulmichl M, Dossena S (2017) PLoS One

Abstract: Lipoyl(Octanoyl) Transferase 2 (LIPT2) is a protein involved in the post-translational modification of key energy metabolism enzymes in humans. Defects of lipoic acid synthesis and transfer start to emerge as causes of fatal or severe early-onset disease. We show that the first 31 amino acids of the N-terminus of LIPT2 represent a mitochondrial targeting sequence and inhibition of the transit of LIPT2 to the mitochondrion results in apoptotic cell death associated with activation of the apoptotic volume decrease (AVD) current in normotonic conditions, as well as over-activation of the swelling-activated chloride current (IClswell), mitochondrial membrane potential collapse, caspase-3 cleavage and nuclear DNA fragmentation. The findings presented here may help elucidate the molecular mechanisms underlying derangements of lipoic acid biosynthesis. β€’ Keywords: HEK 293T β€’ Bioblast editor: Krumschnabel G β€’ O2k-Network Lab: AT Innsbruck Oroboros


Labels: MiParea: Respiration, Genetic knockout;overexpression 

Stress:Cell death  Organism: Human  Tissue;cell: Kidney  Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2017-06