Nemec 2018 MiP2018

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Michal Nemec
Aerobic/strength exercise intervention modulates skeletal muscle mitochondrial function in seniors.

Link: MiP2018

Nemec M, Maderova D, Krumpolec P, Sedliak M, Cvecka J, Tirpakova V, Slobodova L, Schoen M, Kovanicova Z, Sumbalova Z, Ukropcova B, Ukropec J (2018)

Event: MiP2018

COST Action MitoEAGLE

Sedentary behavior increases the age-related risk of neurodegenerative and metabolic diseases. Therefore, we investigated effects of combined aerobic/strength training (n=23) and that of control stretching exercise (n=10) in seniors (28F/6M; age 66.5±4.3; BMI 26.4±4.0) on the functional state of the skeletal muscle mitochondria.

Percutaneous muscle biopsy (m. vastus lateralis) was performed in local anesthesia by Bergström technique. Specific functional parameters of muscle mitochondria were evaluated in saponin-permeabilized muscle fibers by high-resolution respirometry (O2k-Respirometer, Oroboros Instruments, Austria) using SUIT RP1 and RP2 protocols [1]. Oxygen consumption rate was expressed in pmol per mg of tissue wet weight per second. Statistical analysis was performed in SigmaStat v3.5 using Two Way ANOVA with repeated measures and Tuckey Post Hoc analysis.

Regular exercise increased LEAK respiration (NL) with NADH-linked substrates pyruvate+malate (p=0.003) in aerobic/strength exercise group compared to control stretching exercise group (p=0.232). Interestingly, training had no effect on LEAK respiration when fatty acid (octanoyl) (FL) was used as a substrate. Aerobic/strength training had strong positive effect (p<0.001) on electron transfer-pathway state after adding succinate (NSE) compared to stretching-control exercise (p=0.373). Although, regular exercise highly increased (p=0.003) electron transfer-pathway state (FNSE) fueled by electrons from octanoyl oxidation, NADH-linked pathway and succinate, effects of stretching exercise were less pronounced (FNSE, p=0.014). There were no differences between the aerobic/strength exercise and control stretching exercise group before training intervention.

Our work clearly shows that aerobic/strength exercise training improved functional state of skeletal muscle mitochondria, especially succinate-dependent electron transfer through mitochondrial complex II (succinate dehydrogenase).


Bioblast editor: Plangger M, Kandolf G O2k-Network Lab: SK Bratislava Sumbalova Z


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style 


Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 


Coupling state: LEAK  Pathway: F, N, NS, Other combinations  HRR: Oxygraph-2k 


Affiliations

Nemec M(1), Maderová D(1), Krumpolec P(1), Sedliak M(2), Cvečka J(2), Tirpáková V(3), Slobodová L(4), Schön M(4), Kovaničová Z(4), Sumbalová Z(5), Ukropcová B(1,2,4), Ukropec J(1)

  1. Inst Exp Endocrinology, Biomedical Research Center, Slovak Academy Sciences
  2. Fac Physical Education and Sport, Comenius Univ
  3. Inst Sports Medicine and Physical Education, Slovak Medical Univ
  4. Inst Pathological Physiology
  5. Pharmacobiochemical Lab; Fac Medicine, Comenius Univ; Bratislava, Slovakia. - michal.nemec@savba.sk

References and Support

  1. Doerrier C, Sumbalova Z, Krumschnabel G, Hiller E, Gnaiger E (2016) SUIT reference protocol for OXPHOS analysis by high-resolution respirometry. Mitochondr Physiol Network 21.06:1-12.

Grant support: VEGA 2/0107/18; APVV-15-0253, COST CA15203