High-resolution respirometry: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=HRR | |abbr=HRR | ||
|description='''High-resolution respirometry''' ([http://www.oroboros.at/index.php?high-resolution-respirometry HRR]) is based on the [[Oxygraph-2k | |description='''High-resolution respirometry''' ([http://www.oroboros.at/index.php?high-resolution-respirometry HRR]) is based on the [[Oxygraph-2k|OROBOROS Oxygraph-2k]], combining chamber design, application of oxygen-tight materials, electrochemical sensors and electronics, Peltier-temperature control and software features ([[DatLab]]) to obtain a unique level of quantitative resolution of [[oxygen concentration]] and [[oxygen flux]], with a closed-chamber or open-chamber mode of operation ([[Titration-Injection microPump|TIP2k]]). Standardized two-point calibration of the polarographic oxygen sensor ([[POS calibration|static sensor calibration]]), calibration of the sensor response time ([[POS calibration|dynamic sensor calibration]]), and evaluation of [[instrumental background oxygen flux]] (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. | ||
|info=[[MiPNet06.01]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_Respir Physiol]] | |info=[[MiPNet06.01]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_Respir Physiol]] | ||
|type=Respiration | |type=Respiration |
Revision as of 20:16, 3 January 2012
Description
High-resolution respirometry (HRR) is based on the OROBOROS Oxygraph-2k, combining chamber design, application of oxygen-tight materials, electrochemical sensors and electronics, Peltier-temperature control and software features (DatLab) to obtain a unique level of quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation (TIP2k). Standardized two-point calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR.
Abbreviation: HRR
Reference: MiPNet06.01; Gnaiger_2008_POS; Gnaiger_2001_Respir Physiol
MitoPedia methods:
Respirometry,
Fluorometry,
Spectrophotometry