Tan 2011 Inflammation: Difference between revisions
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{{Publication | {{Publication | ||
|title=Tan EC, van Goor H, Bahrami | |title=Tan EC, van Goor H, Bahrami Sl, Kozlov AV, Leixnering M, Redl H, Goris RJ (2011) Intra-arterial tert-Butyl-hydroperoxide infusion induces an exacerbated sensory response in the rat hind limb and is associated with an impaired tissue oxygen uptake. Inflammation 34:49-57. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/20386971 PMID:20386971]; [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021700/?tool=pubmed pdf] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/20386971 PMID: 20386971 Open Access]; [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021700/?tool=pubmed pdf] | ||
|authors=Tan EC, van Goor H, Bahrami | |authors=Tan EC, van Goor H, Bahrami Sl, Kozlov AV, Leixnering M, Redl H, Goris RJ | ||
|year=2011 | |year=2011 | ||
|journal=Inflammation | |journal=Inflammation | ||
|abstract=The objective of this study was to investigate oxidative stress and oxygen extraction mechanisms in an animal model of continuous intra-arterial infusion of a free radical donor and in an in vitro model using isolated mitochondria. tert-Butyl-hydroperoxide (tert-BuOOH, 25 mM) was infused for 24 h in the left hind limb of rats to induce soft tissue damage (''n''โ=โ8). After 7 days, we assessed local sensory response, tissue oxygen consumption, oxygen radicals, and antioxidant levels. In vitro mitochondrial function was measured after stimulation of isolated mitochondria of skeletal muscle cells with increasing doses of tert-BuOOH. tert-BuOOH infusion resulted in an increased skin temperature (''P''โ=โ0.04), impaired function, and a significantly increased pain sensation (''P''โ=โ0.03). Venous oxygen saturation levels (''P''โ=โ0.01) and the antioxidant ceruloplasmin (''P''โ=โ0.04) were increased. tert-BuOOH inhibited mitochondrial function in vitro. Induction of free radical formation in the rat hind limb results in an exacerbated sensory response and is associated with impaired oxygen extraction, which likely results from mitochondrial dysfunction caused by free radicals. | |abstract=The objective of this study was to investigate oxidative stress and oxygen extraction mechanisms in an animal model of continuous intra-arterial infusion of a free radical donor and in an ''in vitro'' model using isolated mitochondria. tert-Butyl-hydroperoxide (tert-BuOOH, 25 mM) was infused for 24 h in the left hind limb of rats to induce soft tissue damage (''n''โ=โ8). After 7 days, we assessed local sensory response, tissue oxygen consumption, oxygen radicals, and antioxidant levels. ''In vitro'' mitochondrial function was measured after stimulation of isolated mitochondria of skeletal muscle cells with increasing doses of tert-BuOOH. tert-BuOOH infusion resulted in an increased skin temperature (''P''โ=โ0.04), impaired function, and a significantly increased pain sensation (''P''โ=โ0.03). Venous oxygen saturation levels (''P''โ=โ0.01) and the antioxidant ceruloplasmin (''P''โ=โ0.04) were increased. tert-BuOOH inhibited mitochondrial function ''in vitro''. Induction of free radical formation in the rat hind limb results in an exacerbated sensory response and is associated with impaired oxygen extraction, which likely results from mitochondrial dysfunction caused by free radicals. | ||
|keywords= | |keywords=Rat model, Oxygen radicals, Oxygen extraction, Mitochondrial dysfunction, Complex regional pain syndrome | ||
|mipnetlab= | |mipnetlab=AT Vienna Kozlov AV | ||
}} | }} | ||
{{Labeling | {{Labeling |
Latest revision as of 14:26, 3 June 2015
Tan EC, van Goor H, Bahrami Sl, Kozlov AV, Leixnering M, Redl H, Goris RJ (2011) Intra-arterial tert-Butyl-hydroperoxide infusion induces an exacerbated sensory response in the rat hind limb and is associated with an impaired tissue oxygen uptake. Inflammation 34:49-57. |
ยป [[Has info::PMID: 20386971 Open Access; pdf]]
Tan EC, van Goor H, Bahrami Sl, Kozlov AV, Leixnering M, Redl H, Goris RJ (2011) Inflammation
Abstract: The objective of this study was to investigate oxidative stress and oxygen extraction mechanisms in an animal model of continuous intra-arterial infusion of a free radical donor and in an in vitro model using isolated mitochondria. tert-Butyl-hydroperoxide (tert-BuOOH, 25 mM) was infused for 24 h in the left hind limb of rats to induce soft tissue damage (nโ=โ8). After 7 days, we assessed local sensory response, tissue oxygen consumption, oxygen radicals, and antioxidant levels. In vitro mitochondrial function was measured after stimulation of isolated mitochondria of skeletal muscle cells with increasing doses of tert-BuOOH. tert-BuOOH infusion resulted in an increased skin temperature (Pโ=โ0.04), impaired function, and a significantly increased pain sensation (Pโ=โ0.03). Venous oxygen saturation levels (Pโ=โ0.01) and the antioxidant ceruloplasmin (Pโ=โ0.04) were increased. tert-BuOOH inhibited mitochondrial function in vitro. Induction of free radical formation in the rat hind limb results in an exacerbated sensory response and is associated with impaired oxygen extraction, which likely results from mitochondrial dysfunction caused by free radicals. โข Keywords: Rat model, Oxygen radicals, Oxygen extraction, Mitochondrial dysfunction, Complex regional pain syndrome
โข O2k-Network Lab: AT Vienna Kozlov AV
Labels:
Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria
HRR: Oxygraph-2k