Coupling Quiz: Difference between revisions
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- After addition of kinetically saturation doses of ADP | - After addition of kinetically saturation doses of ADP | ||
|| The LEAK-state is defined as a state of mitochondrial respiration when | || The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. ย | ||
- After | - After protonophore titration | ||
|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when | || Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. The intrinsic proton leak is the ''uncoupled'' leak current of protons in which protons diffuse across the mtIM in the dissipative direction of the downhill protonmotive force without coupling to phosphorylation. Protonophores are ''uncouplers'' which are titrated to obtain maximum ''noncoupled'' respiration as a measure of ET-capacity. | ||
- In the absence of substrates | - In the absence of substrates | ||
|| Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when | || Wrong answer :( . The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. Therefore substrates are necessary to reach LEAK state of mitochondrial respiration. | ||
+ In the absence of ADP | + In the absence of ADP | ||
|| Correct! The LEAK-state is defined as a state of mitochondrial respiration when | || Correct! The LEAK-state is defined as a state of mitochondrial respiration when O<sub>2</sub> flux mainly compensates for ion leaks in the absence of ATP synthesis, at kinetically-saturating concentrations of O<sub>2</sub>, respiratory fuel substrates and P<sub>i</sub>. In the absence of ADP no ATP synthesis can procede and we measure low, cation leak-dependent respiration. | ||
{How are the units of electric energy [J] and electric force [V] related? | {How are the units of electric energy [J] and electric force [V] related? |
Latest revision as of 12:36, 27 January 2020
Do you feel the Coupling?
- Note: This quiz was made to test your knowledge of mitochondrial terminology of coupling states and to help you understand basic concepts of mitochondrial physiology
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