MiPNet18.06 Amplex-Yeast: Difference between revisions
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. [[Media:MiPNet18.06 Amplex-Yeast.pdf|»Bioblast pdf«]] | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. [[Media:MiPNet18.06 Amplex-Yeast.pdf|»Bioblast pdf«]] | ||
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|topics=Oxygen kinetics, Uncoupler | |topics=Oxygen kinetics, Uncoupler | ||
|couplingstates=ROUTINE, ETS | |couplingstates=ROUTINE, ETS | ||
|instruments=Oxygraph-2k, O2k-Fluorometer, Protocol | |instruments=Oxygraph-2k, O2k-Fluorometer, O2k-Protocol | ||
|additional=O2k-Demo, O2k-MultiSensor | |additional=O2k-Demo, O2k-MultiSensor | ||
}} | }} | ||
{{OROBOROS header page name}} | |||
== See also == | == See also == | ||
* A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - [[MiPNet15.09. Yeast: HRR Reference Assay |»Bioblast link«]] | * A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - [[MiPNet15.09. Yeast: HRR Reference Assay |»Bioblast link«]] |
Revision as of 17:35, 25 January 2016
O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. »Bioblast pdf« |
» Versions
OROBOROS (2015-06-15) Mitochondr Physiol Network
Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2015) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(04):1-4
Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the O2k-Fluorescence LED2-Module. Extracellular H2O2-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H2O2 production (Fig. 2).
- >> Product: OROBOROS O2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom
Labels: MiParea: Respiration
Organism: Saccharomyces cerevisiae
Preparation: Intact cells
Regulation: Oxygen kinetics, Uncoupler Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k, O2k-Fluorometer, O2k-Protocol
O2k-Demo, O2k-MultiSensor
MiPNet18.06 Amplex-Yeast
See also
- A mitochondrial reference assay for high-resolution respirometry using freeze-dried Baker’s yeast. - »Bioblast link«