|
|
Line 1: |
Line 1: |
| [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | | [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] |
| == Steps and respiratory states == | | == Steps and respiratory states == |
| [[File:1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama.png|400px]] | | [[File:1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot-.png.png|400px]] |
|
| |
|
| {| class="wikitable" border="1" | | {| class="wikitable" border="1" |
Revision as of 09:10, 15 April 2023
Steps and respiratory states
File:1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot-.png.png
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1D
|
ROX
|
|
|
1D
- ADP is added to stimulate the consumption of endogenous fuel-substrates.
|
2M.1
|
|
|
|
1D;2M.1
|
3Oct
|
OctMP
|
F
|
FAO
|
1D;2M.1;3Oct
- Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration.
|
3c
|
OctMcP
|
F
|
FAO
|
1D;2M.1;3Oct;3c
- Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
- Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
|
4M2
|
OctMP
|
F(N)
|
FAO
|
1D;2M.1;3Oct;3c;4M2
- Respiratory stimulation of the FAO-pathway, F, by fatty acid FA in the presence of malate M. Malate is a type N substrate (N), required for the F-pathway. In the presence of anaplerotic pathways (e.g., mitochondrial malic enzyme, mtME) the F-pathway capacity is overestimated, if there is an added contribution of NADH-linked respiration, F(N) (see SUIT-002). The FA concentration has to be optimized to saturate the FAO-pathway, without inhibiting or uncoupling respiration.
- High concentration of malate, typically 2 mM, saturates the N-pathway.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
5P
|
OctPMP
|
FN
|
FAO&CI
|
1D;2M.1;3Oct;4M2;5P
- Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
6G
|
OctPGMP
|
FN
|
FAO&CI
|
1D;2M.1;3Oct;4M2;5P;6G
- Respiratory stimulation by simultaneous action of the F-pathway and N-pathway with convergent electron flow in the FN-pathway for evaluation of an additive or inhibitory effect of F.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
7S
|
OctPGMSP
|
FNS
|
FAO&CI&II
|
1D;2M.1;3Oct;4M2;5P;6G;7S
- Respiratory stimulation by simultaneous action of the F-pathway, N-pathway, and S-pathway, with convergent electron flow in the FNS-pathway for reconstitution of TCA cycle function and additive or inhibitory effect of F.
- OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
|
8Gp
|
OctPGMSGpP
|
FNSGp
|
FAO&CI&II&GpDH
|
1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp
|
9U
|
OctPGMSGpE
|
FNSGp
|
FAO&CI&II&GpDH
|
1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U
|
10Rot
|
SGpE
|
SGp
|
CII&GpDH
|
1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot
- Respiratory stimulation by action of succinate and glycerophosphate, Gp, with convergent electron flow in the SGp-pathway (CII&GpDH-linked pathway to the Q-junction).
- Noncoupled electron transfer state, ET state, with ET capacity E.
|
11Ama
|
ROX
|
|
|
1D;2M.1;3Oct;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
|
{{Template:Keywords in SUIT protocols
Cookies help us deliver our services. By using our services, you agree to our use of cookies.