Template:Chemical SUIT-034 NADH mt D082: Difference between revisions
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| | | Reoxygenation can be performed by opening the chamber, see: [[Open chamber]]. | ||
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Revision as of 18:56, 19 December 2023
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1D.1 | ADP (D) | |
2PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
3D2.5 | ADP (D) | |
4U | SF6847 | We do not recommend the use of any other uncoupler, like Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to the chemical background effect on fluorescence. |
5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
6Myx | Myxothiazol | We do not recommend the use of any other inhibitor of complex III, like Antimycin A (Ama), due to the chemical background effect on fluorescence. |
7Reox | Reoxygenation can be performed by opening the chamber, see: Open chamber. |