Template:SUIT text D109: Difference between revisions
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The coupling-control protocol SUIT-032 O2 mt D109 is used as a control protocol for [[SUIT-032 NADH mt D078]], allowing for cytochrome ''c'' test and the evaluation of mitochondrial respiration in two [[coupling control]] states: [[LEAK]], and [[OXPHOS]] in the [[N-pathway]] | The coupling-control protocol SUIT-032 O2 mt D109 is used as a control protocol for [[SUIT-032 NADH mt D078]], allowing for cytochrome ''c'' test and the evaluation of mitochondrial respiration in two [[coupling control]] states: [[LEAK]], and [[OXPHOS]] in the [[N-pathway]] | ||
After the addition of mitochondria in the absence of fuel substrates and ADP, [[Ren|''Ren'']], respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured | After the addition of mitochondria in the absence of fuel substrates and ADP, [[Ren|''Ren'']], respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured. | ||
The addition of the complex III inhibitor myxothiazol allows the measurement of [[ROX|''Rox'']]. | The addition of the complex III inhibitor myxothiazol allows the measurement of [[ROX|''Rox'']]. | ||
Latest revision as of 12:37, 5 January 2024
The coupling-control protocol SUIT-032 O2 mt D109 is used as a control protocol for SUIT-032 NADH mt D078, allowing for cytochrome c test and the evaluation of mitochondrial respiration in two coupling control states: LEAK, and OXPHOS in the N-pathway
After the addition of mitochondria in the absence of fuel substrates and ADP, Ren, respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured.
The addition of the complex III inhibitor myxothiazol allows the measurement of Rox.
