From Bioblast
Description
Octanoylcarnitine is a medium-chain fatty acid (octanoic acid: eight-carbon saturated fatty acid) covalently linked to carnitine, frequently applied as a substrate for fatty acid oxidation (FAO) in mitochondrial preparations.
Abbreviation: Oct
Reference: pdf
MitoPedia topics: Substrate and metabolite
Application in HRR
- Oct: Octanoylcarnitine ((Β±)-Octanoylcarnitine chloride; C15H30NO4Cl), BioTrend (brand: APExBIO Technology): B6371-50 mg, store at -20 Β°C, CAS: 18822-86-1, M = 323.86 gΒ·mol-1
- Alternative: TOCRIS Bioscience, Cat. No. 0605, 50 mg, store at RT, desiccate); M = 323.85 gΒ·mol-1.
- General tips: For obtaining a higher solubility, warm the tube at 37 β and shake it in the ultrasonic bath for a while.
- Preparation of 100 mM stock solution (dissolved in H2O or DMSO, can differ from batch to batch - check Certificate of Analysis):
- Weigh 50 mg of octanoylcarnitine and dissolve in 1543 Β΅L H2O or DMSO.
- Divide into 0.2 mL portions.
- Store at -20 Β°C.
- Note 2024-03-26: According to the information provided by the supplier (BioTrend) is the solubility in DMSO and H2O comparable, therefore we consider more rational approach to use H2O as a solvent due to higher melting point and possible toxic effects of DMSO,
- Β» O2k manual titrations into 2 mL O2k-chamber
- Titration volume (2-mL O2k-chamber): 10 Β΅L using a 25 Β΅L Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 Β΅L using a 10 Β΅L Hamilton syringe
- Final concentration: 0.5 mM in MiR05 or MiR06. Optimum Oct concentrations have to be evaluated, particularly in media with BSA concentrations different from MiR05.
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MitoPedia:FAT4BRAIN