Pinho 2011 Abstract IOC65
Pinho JPC, Goncalves RLS, Oliveira MF (2011) Studies on the dynamic changes of mitochondrial function following a blood meal in the kissing bug Rhodnius prolixus. IOC64. |
Link:
Pinho JPC, Goncalves RLS, Oliveira MF (2011)
Event: IOC64
The physiological implications of hematophagy in arthropods raise interest because many of these organisms are vectors of important diseases. Evidence shows that blood-feeding organisms developed adaptive mechanisms to avoid the toxic products of blood digestion. Since oxidative metabolism is an important source of reactive oxygen species (ROS), we investigated here the hypothesis that the insect Rhodnius prolixus would modulate its mitochondrial function after a blood meal (ABM). We have employed respirometry and spectrofluorimetry to assess oxygen consumption and hydrogen peroxide generation in adult females of R. prolixus flight muscle, respectively. We observed that insects decreased both respiration and hydrogen peroxide (H2O2) production in several mitochondrial metabolic states ABM. The activities of complexes I-III and IV were not distinct ABM, indicating that respiratory chain was not involved on the reduced respiration rates observed. Blood-feeding caused a reversible reduction in mitochondrial oxygen consumption, an event that was parallel to blood digestion, being most intense 7 days ABM. We also observed a decrease in 78% and 65% in ADP-induced and uncoupled respiration, respectively, throughout the insect life-span. We conclude that blood feeding plays a major role in regulating mitochondrial function and may represent an important adaptation of this insect to hematophagy.
β’ Keywords: "Rhodnius prolixus", hematophagy, ROS
Labels:
Stress:RONS; Oxidative Stress Organism: Other Non-Mammal
Preparation: Isolated Mitochondria, Enzyme Enzyme: TCA Cycle and Matrix Dehydrogenases, Complex I, Complex III, Complex IV; Cytochrome c Oxidase Regulation: Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential, Substrate; Glucose; TCA Cycle, Redox State
HRR: Oxygraph-2k, Spectrophotometry, Spectrofluorimetry