Difference between revisions of "Bioblast alert 2011"
From Bioblast
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'''Bioblast alert 2011: 01 to 04''' | '''Bioblast alert 2011: 01 to 04''' >> [[Bioblast alert|Current issue]] | ||
=== Bioblast alert 2011( | === Bioblast alert 2011(01): 2011-11-11 === | ||
* | * A key question on the separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls? | ||
::[[Pesta_2011_Am J Physiol Regul Integr Comp Physiol|Pesta D, Hoppel F, Macek C, Messner H, Faulhaber M, Kobel C, Parson W, Burtscher M, Schocke M, Gnaiger E (2011) Similar qualitative and quantitative changes of mitochondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1078–R1087.]] | |||
* [[ETS capacity]] plays a key role in such analyses, which cannot be achieved quantitatively without careful '''[[Optimum uncoupler concentration|uncoupler titrations]]'''. | |||
* '''[[Basal respiration]]''' - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology. | |||
*'''[[ | |||
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* | === Bioblast alert 2011(03): 2011-11-24 === | ||
* [[ | * '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118-124.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B [Epub ahead of print] ]]</ref>. | ||
* '''[[ | <references/> | ||
*'''[[Talk:Fluorometry|Fluorometry versus fluorimetry]]''' - term of the week. >>>New '''[[Glossary: Fluorometry]]''' - high-resolution terminology. | |||
=== Bioblast alert 2011(04): 2011-12-24 === | |||
* '''Congratulations''' to [[Pesta D|Dr. Dominik Pesta]], for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012) | |||
* [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]] | |||
* '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''': | |||
::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] | |||
[[Category:Gentle Science]] | |||
Revision as of 07:15, 18 April 2014
Bioblast alert is prepared by the Bioblast editorial board to draw attention to specific topics on the Bioblast wiki.
Bioblast alert: for information purposes, not intended to be an advertisement.
Bioblast alert 2011: 01 to 04 >> Current issue
Bioblast alert 2011(01): 2011-11-11
- A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
- ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
- Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
Bioblast alert 2011(02): 2011-11-17
- Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism.
- Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols. (Scientific service)
- Uncoupled, non-coupled, dyscoupled - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
- Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.
Bioblast alert 2011(03): 2011-11-24
- Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper[1], or optical probe operated through the O2k-glass window[2].
- ↑ Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118-124.
- ↑ Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B [Epub ahead of print]
- Fluorometry versus fluorimetry - term of the week. >>>New Glossary: Fluorometry - high-resolution terminology.
Bioblast alert 2011(04): 2011-12-24
- Congratulations to Dr. Dominik Pesta, for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
- Bioblast editorial board - Why a wiki editorial board?
- SUIT protocols for HRR: from intact cells to permeabilized muscle fibres:
