Difference between revisions of "Bioblast alert 2011"
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=== Bioblast alert 2011(03): 2011-11-24 === | === Bioblast alert 2011(03): 2011-11-24 === | ||
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* '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300: H118- | * '''Fluorometry combined with high-resolution respirometry''': Light guide inserted into the chamber through a black PEEK stopper<ref>[[Anderson 2011 Am J Physiol Heart Circ Physiol|Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.]]</ref>, or optical probe operated through the O2k-glass window<ref>[[Hickey 2012 J Comp Physiol B|Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (''Hemiscyllum ocellatum''). J Comp Physiol B 182:91-100. ]]</ref>. | ||
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* [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]] Β | * [[Bioblast editorial board]] - [[Talk:Bioblast editorial board|Why a wiki editorial board?]] Β | ||
* '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''': | * '''[[SUIT protocol]]s for HRR: from intact cells to permeabilized muscle fibres''': | ||
::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]] | ::[[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58.]] | ||
[[Category:Gentle Science]] | [[Category:Gentle Science]] |
Revision as of 11:22, 3 March 2015
Bioblast alert is prepared by the Bioblast editorial board to draw attention to specific topics on the Bioblast wiki.
Bioblast alert: for information purposes, not intended to be an advertisement.
Bioblast alert 2011: 01 to 04 >> Current issue
Bioblast alert 2011(01): 2011-11-11
- A key question on the separation of qualitative versus quantitative change of mitochondrial function is addressed in a new O2k-Publication. Does training induce mild uncoupling? Are sedentary humans healthy controls?
- ETS capacity plays a key role in such analyses, which cannot be achieved quantitatively without careful uncoupler titrations.
- Basal respiration - term of the month: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
Bioblast alert 2011(02): 2011-11-17
- Heart failure and mitochondrial dysfunction. Increased OXPHOS capacity after cold preservation of the human heart β a paradox resolved by a dyscoupling mechanism.
- Tissue storage on ice is possible for limited periods of time (several hours) with preservation of mitochondrial respiratory function, as evaluated with SUIT protocols.
- Uncoupled, noncoupled, dyscoupled - term of the week: a hallmark of high-resolution respirometry is a matching high-resolution terminology.
- Evaluate an example of low-resolution terminology: Uncoupling was studied in stressed cells by measurement of coupled respiration, evaluation of uncoupled respiration after inhibition with oligomycin, and FCCP titration to quantify uncoupled respiration.
Bioblast alert 2011(03): 2011-11-24
- Fluorometry combined with high-resolution respirometry: Light guide inserted into the chamber through a black PEEK stopper[1], or optical probe operated through the O2k-glass window[2].
- β Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP (2011) Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 300:H118-24.
- β Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J Comp Physiol B 182:91-100.
- Fluorometry versus fluorimetry - term of the week. >>>New MitoPedia: Fluorometry - high-resolution terminology.
Bioblast alert 2011(04): 2011-12-24
- Congratulations to Dr. Dominik Pesta, for receiving his degree of a PhD at the Medical University of Innsbruck! (Jan 2012)
- Bioblast editorial board - Why a wiki editorial board?
- SUIT protocols for HRR: from intact cells to permeabilized muscle fibres: