Difference between revisions of "Bioblast quiz"
From Bioblast
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|| Understanding the P/O ratio's implications on mitochondrial efficiency is crucial for assessing bioenergetic health. | || Understanding the P/O ratio's implications on mitochondrial efficiency is crucial for assessing bioenergetic health. | ||
{'''Assuming the standard reduction potential (E°') for NADH → NAD+ is -0.320 V and for O<sub>2</sub> → H<sub>2</sub>O is +0.815 V, calculate the ΔE°' for the electron transport from NADH to O<sub>2</sub>. What does ΔE°' indicate about the potential energy available for ATP synthesis?''' | {'''Assuming the standard reduction potential (E°') for NADH → NAD<sup>+</sup> is -0.320 V and for O<sub>2</sub> → H<sub>2</sub>O is +0.815 V, calculate the ΔE°' for the electron transport from NADH to O<sub>2</sub>. What does ΔE°' indicate about the potential energy available for ATP synthesis?''' | ||
|type="()"} | |type="()"} | ||
+ ΔE°' = 1.135 V; indicates a high potential energy available for ATP synthesis | + ΔE°' = 1.135 V; indicates a high potential energy available for ATP synthesis | ||
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|| The calculation of ΔE°' provides | || The calculation of ΔE°' provides | ||
{'''If the inner mitochondrial membrane has a surface area of 5.0 × 10<sup>6</sup> μm<sup>2</sup> per mg of protein and each Complex I can pump 4 protons across the membrane, how many protons are pumped per second assuming a turnover number of 100 | {'''If the inner mitochondrial membrane has a surface area of 5.0 × 10<sup>6</sup> μm<sup>2</sup> per mg of protein and each Complex I can pump 4 protons across the membrane, how many protons are pumped per second assuming a turnover number of 100 · s<sup>-1</sup> for Complex I?''' | ||
|type="()"} | |type="()"} | ||
- 2.0 | - 2.0 · 10<sup>9</sup> protons · s<sup>-1</sup> | ||
|| Without knowing the density of Complex I on the membrane, the calculation of protons pumped is speculative. | || Without knowing the density of Complex I on the membrane, the calculation of protons pumped is speculative. | ||
- 5.0 | - 5.0 · 10<sup>9</sup> protons · s<sup>-1</sup> | ||
|| Without knowing the density of Complex I on the membrane, the calculation of protons pumped is speculative. | || Without knowing the density of Complex I on the membrane, the calculation of protons pumped is speculative. | ||
- 2.0 | - 2.0 · 10<sup>9</sup> protons · s<sup>-1</sup> | ||
|| Without knowing the density of Complex I on the membrane, the calculation of protons pumped is speculative. | || Without knowing the density of Complex I on the membrane, the calculation of protons pumped is speculative. | ||
+ Calculation cannot be completed without the number of Complex I per μm<sup>2</sup> | + Calculation cannot be completed without the number of Complex I per μm<sup>2</sup> | ||
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|| Precise calculation based on the given variables and constants illustrates a fundamental understanding of bioenergetic principles. | || Precise calculation based on the given variables and constants illustrates a fundamental understanding of bioenergetic principles. | ||
{'''The efficiency of mitochondrial oxidative phosphorylation can be described by the equation η = (ΔG_ATP/ΔG_O2) × 100%, where ΔG_ATP is the free energy change for ATP synthesis, and | {'''The efficiency of mitochondrial oxidative phosphorylation can be described by the equation η = (ΔG_ATP/ΔG_O2) × 100%, where ΔG_ATP is the free energy change for ATP synthesis, and ΔG_O<sub>2</sub> is the free energy change for oxygen reduction. If ΔG_ATP = -50 kJ/mol and ΔG_O<sub>2</sub> = -200 kJ/mol, what is the efficiency (η) of oxidative phosphorylation?''' | ||
|type="()"} | |type="()"} | ||
- 25 % | - 25 % | ||
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{'''Consider a mitochondrial uncoupling scenario where the membrane potential (Δψ) is decreased by 50% without altering the proton gradient (ΔpH). Using the Nernst equation for protons, E = (RT/zF)ln([H+]out/[H+]in), predict how this change affects the pmF. Assume R, T, F, and z values remain constant.''' | {'''Consider a mitochondrial uncoupling scenario where the membrane potential (Δψ) is decreased by 50 % without altering the proton gradient (ΔpH). Using the Nernst equation for protons, E = (RT/zF)ln([H+]out/[H+]in), predict how this change affects the pmF. Assume R, T, F, and z values remain constant.''' | ||
|type="()"} | |type="()"} | ||
- pmF decreases by 50 % | - pmF decreases by 50 % |
Revision as of 13:32, 5 April 2024
Self educational quizzes
The Bioblast quiz has been initiated by Ondrej Sobotka.
- For tips&tricks and detailed instructions about how to make a quiz visit links below:
Exemplary quiz
- Note: Questions in this exemplary quiz were used from a set of questions prepared for the MiPschool Tromso-Bergen 2018: The protonmotive force and respiratory control. 1. Coupling of electron transfer reactions to vectorial translocation of protons. 2. From Einstein’s diffusion equation on gradients to Fick’s law on compartments. - Gnaiger 2018 MiPschool Tromso A2
- Only one correct answer.
List of Quizzes on Bioblast
- Please link your quizzes to this page and feel free to contribute!
Blue Book Bioblast Quiz
Blue Book chapter 1: basic questions
Blue Book chapter 1: Advanced questions
Chapter 1.2 specific questions