Coupling-control ratio

high-resolution terminology - matching measurements at high-resolution

Coupling-control ratio

Description

Coupling control ratios, CCR, are flux control ratios, FCR, at a constant mitochondrial pathway control state. In mitochondrial preparations, there are three well-defined coupling states of respiration, L, P, E (LEAK, OXPHOS, ET-pathway). In intact cells, state P cannot be induced, but a ROUTINE state of respiration, R, can be measured. The reference state, J_ref, is defined by taking J_ref as the maximum flux, i.e. flux in the ET state, E, such that the lower and upper limits of the CCR are defined as 0.0 and 1.0. Then there are two mitochondrial CCR, L/E and P/E, and two CCR for intact cells, L/E and R/E.

Abbreviation: CCR

Reference: Flux control ratio

The Blue Book 2014: Fig. 2.4.

Coupling control ratios related to free capacities

• L/P coupling control ratio, L/P

Free OXPHOS capacity, ≈P = P-L

OXPHOS coupling efficiency, j_≈P = ≈P/P = 1-L/P

• L/R coupling control ratio, L/R

Free ROUTINE activity, ≈R = R-L

ROUTINE coupling efficiency, j_≈R = ≈R/R = 1-L/R

• LEAK control ratio, L/E

Free ET-capacity, ≈E = E-L

ET-coupling efficiency, j_≈E = ≈E/E = 1-L/E

• netOXPHOS control ratio, ≈P/E=(P-L)/E

Free OXPHOS capacity, ≈P = P-L

• netROUTINE control ratio, ≈R/E=(R-L)/E

Free ROUTINE activity, ≈R = R-L

• More details » ET-coupling efficiency

Coupling control ratios related to excess capacities

• OXPHOS control ratio, P/E

Excess E-P capacity, ExP = E-P

Excess E-P capacity factor, j_ExP = ExP/E = 1-P/E

• ROUTINE control ratio, R/E

Excess E-R capacity, ExR = E-R

Excess E-R capacity factor, j_ExR = ExR/E = 1-R/E

Compare

» Coupling control factor, CCF = 1-CCR

» Respiratory acceptor control ratio, RCR

References

Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Rate

» Normalization of rate

» Flow

» Oxygen flow

» Flux

» Oxygen flux

» Flux control ratio

» Coupling-control ratio

» Pathway control ratio

» Flux control efficiency

Quantities for normalization

» Count in contrast to Number

» Mitochondrial marker

» O2k-Protocols: mitochondrial and marker-enzymes

» Citrate synthase activity

General

» Extensive quantity

» Specific quantity

» Advancement

» Motive unit

» Iconic symbols

Related keyword lists

» Keywords: Concentration and pressure

MitoPedia concepts: Respiratory control ratio 

MitoPedia methods: Respirometry