From Bioblast
- high-resolution terminology - matching measurements at high-resolution
Flux analysis - DatLab
Description
The strategy of DatLab Flux analysis depends on the research question and the corresponding settings applied in DatLab when recording the data. Frequently a sequence of respiratory steady-states is measured, marks are set, and numerical data are summarized in Mark statistics (F2). An AI approach is kept in mind when describing guidelines for evaluation of steady-states during data recording and data analysis.
Reference: MitoPedia: DatLab
MitoPedia O2k and high-resolution respirometry:
DatLab
Step analysis and state analysis
- In a SUIT protocol each titration can be considered as a transition bewtween a background state and a reference state, expressed as a flux control factor, FCF. An ideal sequence of steady-state states can be analysed in terms of flux control ratios, FCR, corrected for a common baseline, and normalized for flux in a common reference state. If a steady-state (constant) flux is not reached between sequential respiratory states induced by titrations, some corrections for dynamic changes of flux may be applied, or the conditions for steady-state analysis break down. In such cases, step analysis with continuous evaluation of a change of flux (second time derivative) may provide the only reliable information. In reality some noise or bias is included in steady-state analysis above a threshold level, below which such data sets would be excluded from state-analysis.
