Difference between revisions of "Hogeboom 1950 J Biol Chem"
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SomeĀ ofĀ theĀ difficultiesĀ involvedĀ inĀ theĀ interpretationĀ ofĀ dataĀ obtainedĀ withĀ theĀ cellĀ fractionationĀ techniqueĀ areĀ presented,Ā andĀ itĀ isĀ concludedĀ thatĀ severalĀ definiteĀ conditionsĀ mustĀ beĀ fulfilledĀ beforeĀ aĀ biochemicalĀ propertyĀ canĀ beĀ ascribedĀ toĀ aĀ givenĀ structuralĀ componentĀ ofĀ theĀ cell. | SomeĀ ofĀ theĀ difficultiesĀ involvedĀ inĀ theĀ interpretationĀ ofĀ dataĀ obtainedĀ withĀ theĀ cellĀ fractionationĀ techniqueĀ areĀ presented,Ā andĀ itĀ isĀ concludedĀ thatĀ severalĀ definiteĀ conditionsĀ mustĀ beĀ fulfilledĀ beforeĀ aĀ biochemicalĀ propertyĀ canĀ beĀ ascribedĀ toĀ aĀ givenĀ structuralĀ componentĀ ofĀ theĀ cell. | ||
|keywords=socitric dehydrogenase, triphosphopyridine nucleotide-cytochrome c reductase | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|organism=Mouse | |||
|tissues=Hepatocyte; Liver | |||
|preparations=Isolated Mitochondria | |||
|enzymes=Complex IV; Cytochrome c Oxidase | |||
|kinetics=Reduced Substrate; Cytochrome c | |||
|additional=Made history | |additional=Made history | ||
}} | }} |
Revision as of 15:58, 9 June 2012
Hogeboom GH, Schneider WC (1950) Cytochemical studies of mammalian tissues III. Isocitric dehydrogenase and triphosphopyridine nucleotide-cytochrome c reductase of mouse liver. J Biol Chem 186: 417-427. |
Ā» PMID: 14794638; Open Access
Hogeboom GH, Schneider WC (1950) J Biol Chem
Abstract: A study is reported of the distribution of isocitric dehydrogenase and TPN-cytochrome c reductase among fractions isolated by differential centrifugation from homogenates of C3H mouse liver.
Over 80 per cent of the total isocitric dehydrogenase activity of the homogenates was recovered in a fraction containing the soluble material of the cytoplasm of the liver cell. Of the particulate fractions, nuclei and submicroscopic particles showed very little activity, whereas mitochondria contained approximately 12 per cent of the total. Results obtained with hypertonic (0.88 M) sucrose as the medium were essentially the same as those obtained with isotonic (0.25 M) sucrose.
Of the total TPN-cytochrome c reductase activity of the homogenates, over 90 per cent was recovered in the particulate fractions, 49 per cent being present in mitochondria and 36 per cent in submicroscopic particles. Under the conditions of the methods of assay, the specific TPN-cytochrome c reductase activity of mouse liver was found to be much lower than values previously reported for DPN-cytochrome c reductase.
Some of the difficulties involved in the interpretation of data obtained with the cell fractionation technique are presented, and it is concluded that several definite conditions must be fulfilled before a biochemical property can be ascribed to a given structural component of the cell. ā¢ Keywords: socitric dehydrogenase, triphosphopyridine nucleotide-cytochrome c reductase
Labels:
Organism: Mouse
Tissue;cell: Hepatocyte; Liver"Hepatocyte; Liver" is not in the list (Heart, Skeletal muscle, Nervous system, Liver, Kidney, Lung;gill, Islet cell;pancreas;thymus, Endothelial;epithelial;mesothelial cell, Blood cells, Fat, ...) of allowed values for the "Tissue and cell" property.
Preparation: Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Enzyme: Complex IV; Cytochrome c Oxidase"Complex IV; Cytochrome c Oxidase" is not in the list (Adenine nucleotide translocase, Complex I, Complex II;succinate dehydrogenase, Complex III, Complex IV;cytochrome c oxidase, Complex V;ATP synthase, Inner mt-membrane transporter, Marker enzyme, Supercomplex, TCA cycle and matrix dehydrogenases, ...) of allowed values for the "Enzyme" property.
Made history