Horseradish peroxidase: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
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:::: According to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions. | :::: According to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions. | ||
{{Keywords: H2O2}} | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 00:21, 18 February 2020
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C.
Preparation of 500 U/ml stock solution (dissolved in MiR05):
- Weigh 3,33 mg
- Dissolve in 1 mL MiR05.
- Aliquot 200 µL into 500 µL Eppendorf tubes.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.
