Horseradish peroxidase: Difference between revisions
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::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C. | ::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C. | ||
'''Preparation of 500 U/mL stock solution''' (dissolved in MiR05): | :::: '''Preparation of 500 U/mL stock solution''' (dissolved in MiR05): | ||
::::# Weigh 3,33 mg | ::::# Weigh 3,33 mg | ||
Revision as of 16:51, 16 September 2020
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
Application in HRR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C.
- Preparation of 500 U/mL stock solution (dissolved in MiR05):
- Weigh 3,33 mg
- Dissolve in 1 mL MiR05.
- Aliquot 200 µL into 500 µL Eppendorf tubes.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.
MitoPedia methods: Fluorometry
