Horseradish peroxidase: Difference between revisions
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|description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | |description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | ||
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{{MitoPedia methods | |||
|mitopedia method=Fluorometry | |||
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{{MitoPedia O2k and high-resolution respirometry}} | |||
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__TOC__ | __TOC__ | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma | ::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P8250, store at 4 °C. | ||
'''Preparation of 500 U/mL stock solution''' (dissolved in MiR05): | :::: '''Preparation of 500 U/mL stock solution''' (dissolved in MiR05): | ||
::::# Weigh 3 | ::::# Weigh 3.33 mg | ||
::::# Dissolve in 1 mL MiR05. | ::::# Dissolve in 1 mL MiR05. | ||
::::# Aliquot 200 µL into 500 µL Eppendorf tubes. | ::::# Aliquot 200 µL into 500 µL Eppendorf tubes. | ||
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== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 15:58, 4 April 2024
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods: Fluorometry
Application in HRR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P8250, store at 4 °C.
- Preparation of 500 U/mL stock solution (dissolved in MiR05):
- Weigh 3.33 mg
- Dissolve in 1 mL MiR05.
- Aliquot 200 µL into 500 µL Eppendorf tubes.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.
