MiPNet22.04 IOC120 Barcelona ES: Difference between revisions
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:::: Facultat de Biologia | :::: Facultat de Biologia | ||
:::: Universitat de Barcelona | :::: Universitat de Barcelona | ||
:::: c/ Baldiri Reixac, 10-12, 08028 | |||
:::: Barcelona, Spain | |||
== General information == | == General information == | ||
| Line 25: | Line 28: | ||
== Registration == | == Registration == | ||
::::* Separate registration: will be available soon. | ::::* Separate registration: will be available soon. | ||
== Programme == | == Programme == | ||
::::'' | {| class='wikitable' | ||
|- | |||
|style="width: 10em"|'''Monday 20th''' | |||
|style="width: 60em"|'''Topic''' | |||
|style="width: 15em"|'''Weblink''' | |||
|- | |||
|08:45 | |||
|Registration | |||
| | |||
|- | |||
|09:00-09:15 | |||
|Welcome by Prof. Antonio Zorzano. | |||
| | |||
|- | |||
|09:15-09:30 | |||
|'''Introduction''' of participants and their research interests. | |||
|[[MiPNet22.04 IOC120 Barcelona ES|IOC120]] | |||
|- | |||
|09:30-10:00 | |||
|'''Get started with the O2k''': Overview with video clips. | |||
|[[O2k-Manual]] | |||
|- | |||
|10:00-11:30 | |||
|'''Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay.''' | |||
|[[Gnaiger 2014 MitoPathways|The Blue Book]] - [[SUIT reference protocol|SUIT reference protocol]] | |||
|- | |||
|11:30-12:00 | |||
|Coffee/tea break | |||
| | |||
|- | |||
|12:00-13:00 | |||
|'''Principles of high-resolution respirometry''': From switching | |||
on the O2k to the experimental result – oxygen sensor calibration and quality control. | |||
|[[MiPNet06.03 POS-calibration-SOP|POS-calibration-SOP]] | |||
|- | |||
|13:00-14:00 | |||
|'''O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol.''' | |||
|[[MiPNet20.14 AmplexRed H2O2-production|O2k-Fluorometry]] | |||
|- | |||
|14:00-15:00 | |||
|Lunch | |||
| | |||
|- | |||
|15:00-17:30 | |||
|'''Demo-Experiment''': HRR and O2k-Fluorometry with permeabilized cells – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2. | |||
|[[SUIT FNSGp(PGM)01|RP1]] - [[SUIT FNSGp(PGM)02|RP2]] | |||
|- | |||
|17:30-18:30 | |||
|'''Protocol driver for Marks and data analysis. ''' | |||
| | |||
|- | |||
|18:30-20:00 | |||
|'''The Bioblast wiki, O2k-Network and feedback discussion.''' | |||
| | |||
|- | |||
|} | |||
== Lecturers and tutors == | |||
<br/> | |||
<gallery mode=packed widths="190px" heights="165px" perrow=6> | |||
File:Gnaiger Erich.jpg|'''[[Gnaiger E|Gnaiger Erich]]''' | |||
File:DoerrierC.JPG|'''[[Doerrier Velasco CA|Doerrier Velasco Carolina]]''' | |||
File:Zorzano sebastian.jpg|'''[[Sebastian D|Sebastián David]]''' | |||
</gallery> | |||
== Contact == | == Contact == | ||
Revision as of 18:11, 27 February 2017
MiPNet22.04 IOC120 Barcelona ES
 Barcelona ES, 2017 Mar 20. O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry IOC120. »ES Barcelona Zorzano A |
OROBOROS (2017-03-20) Mitochondr Physiol Network
Abstract:
O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry - satellite to MITOEAGLE Barcelona 2017. Barcelona, Spain; 2017 Mar 20.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, ES Barcelona Zorzano A
Venue
- ES Barcelona Zorzano A
- Institute for Research in Biomedicine (IRB Barcelona)
- Facultat de Biologia
- Universitat de Barcelona
- c/ Baldiri Reixac, 10-12, 08028
- Barcelona, Spain
General information
- O2k-Fluorometer demo experiment: high-resolution respirometry and H2O2 production (Amplex red)
- Followed by the Barcelona MITOEAGLE Conference, MG and WC Meeting.
Registration
- Separate registration: will be available soon.
Programme
| Monday 20th | Topic | Weblink |
| 08:45 | Registration | |
| 09:00-09:15 | Welcome by Prof. Antonio Zorzano. | |
| 09:15-09:30 | Introduction of participants and their research interests. | IOC120 |
| 09:30-10:00 | Get started with the O2k: Overview with video clips. | O2k-Manual |
| 10:00-11:30 | Comprehensive OXPHOS analysis: substrate-uncoupler-inhibitor titration (SUIT) protocols for respiratory control by coupling and mitochondrial pathways, SUIT reference assay. | The Blue Book - SUIT reference protocol |
| 11:30-12:00 | Coffee/tea break | |
| 12:00-13:00 | Principles of high-resolution respirometry: From switching
on the O2k to the experimental result – oxygen sensor calibration and quality control. |
POS-calibration-SOP |
| 13:00-14:00 | O2k-Fluorometry and OXPHOS analysis: Amplex red assay of H2O2 production and design of experimental protocol. | O2k-Fluorometry |
| 14:00-15:00 | Lunch | |
| 15:00-17:30 | Demo-Experiment: HRR and O2k-Fluorometry with permeabilized cells – respiration and H2O2 production applying the SUIT reference assay: Protocol driver for RP1&RP2. | RP1 - RP2 |
| 17:30-18:30 | Protocol driver for Marks and data analysis. | |
| 18:30-20:00 | The Bioblast wiki, O2k-Network and feedback discussion. |
Lecturers and tutors
Contact
- OROBOROS INSTRUMENTS
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2017, Next, O2k-Network
