Michalak Slawomir

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Working Groups

Short-Term Scientific Missions

Management Committee

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Michalak Slawomir

MitoPedia topics: MitoPedia topic::EAGLE 

COST: Has COST::Member

COST WG4: Has COST WG4::WG4 COST Mentor: Has COST Mentor::Mentor

MC Member - Management Committee MitoEAGLE

Name	Has lastname::Michalak Has firstname::Slawomir, Has title::Dr
Institution	[[Has institution:: Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, PL]]
Address	Has address::Przybyszewskiego Str. 49, Has area code::60-355
City	In city::Poznan
State/Province	In state::
Country	In country::Poland
Email	Has mailaddress::[email protected]
Weblink	Weblink::
O2k-Network Lab	{{#ask:has member::Michalak Slawomir}}

Labels: Field of research: field of research::Basic, field of research::Clinical 

Publications

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Abstracts

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MitoEAGLE Short-Term Scientific Mission

• • • • STSM Grant Period 2

Work plan summary

1. Aim and motivation. Blood cells, like peripheral mononuclear cells (PBMCs) and platelets, can be obtained with minimal invasive procedures and used for analyzes of mitochondrial respiration for diagnostic and research purposes. Up to now I have analyzed by means of ELISA technique the expression of mitochondrial proteins in PBMCs from movement disorders and cancer patients. With the use of high-resolution respirometry I have analyzed mitochondrial respiration in PBMCs in movement disorders and cancer patients. The results were already published. Moreover, I have performed methodological study on the effects of anticoagulants on mitochondrial respiration in PBMCs. The results of the studies in movement disorders patients and on anticoagulant effects were my contributions to the Working Group 4 in COST Action MITOEAGLE. Currently, I do have the opportunity to perform analyzes of Ca+2 ions and mitochondrial membrane potential basing on tetraphenylphosphonium (TPP+) or TPMP+ evaluations using potentiometric ion-selective electrodes (ISE) and of reactive oxigen species using the system for high-resolution respirometry (HRR). However, ISE and HRR techniques require specific skills I would like to acquire during STSM I apply to and it is the aim of this STMS.

2. Proposed contributions to the scientific objectives of the Action I will contribute the results of the studies on calcium flow, mitochondrial membrane potential and production of reactive oxigen species in PBMCs in physiological and pathological conditions to the repository of Working Group 4 in COST Action MITOEAGLE. Moreover, I will continue the studies on methodology and protocols for mitochondrial analyzes in PBMCs using new techniques I will learn during STSM.

3. Techniques The techniques I will learn during STSM include potentiometric analyses using ion-selective electrodes (ISE) and high-resolution respirometry by means of MultiSensor applications. My training during STSM will fully contribute to the scientific objectives of MitoEAGLE according to the Memorandum of Understanding.

MitoEAGLE feedback

• I would like to participate in WG4 - MitoEAGLE data repository for blood cells and clutured cells. We are already working with cryopreserved PBMC from lung cancer, breast cancer and colon cancer patients. Thus, I would like to participate in WG4 tasks. - Slawomir Michalak (2016).

Participated at

• MitoEAGLE 2019 Belgrade RS
• ESCI 2019 Coimbra PT
• MitoEAGLE 2019 Matrei am Brenner AT
• MitoEAGLE 2018 Jurmala LV
• MitoEAGLE 2018 Lund SE
• MitoEAGLE 2018 Poznan PL
• MitoEAGLE 2018 AT
• IOC126 Innsbruck AT
• MitoEAGLE 2017 Hradec Kralove CZ
• MitoEAGLE 2017 Barcelona ES
• MitoEAGLE 2016 Verona IT
• IOC100 Schroecken AT
• IOC80 Schroecken AT

Visiting scientist in the Oroboros O2k-Laboratory

Slawomir Michalak : Visiting scientist at the Oroboros O2k-Laboratory

• January 23 to February 03 2018