Difference between revisions of "MitoEAGLE data: blood and cultured cells"
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Revision as of 14:58, 27 April 2016
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Hoppel Florian Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Gnaiger Erich Austria Innsbruck Fischer Christine Austria Innsbruck Meszaros Andras T Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Doerrier Carolina Austria Innsbruck Torres-Quesada Omar Austria Innsbruck Karabatsiakis Alexander Belgium Namur Detraux Damien Belgium Liege Votion Dominique-Marie Belgium Leuven Bird Matthew Belgium Namur Arnould Thierry Belgium Namur Renard P Bosnia and Herzegovina Tuzla Jusic Amela Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil Sao Paulo Dominguez-Amorocho OA Brazil Sao Paulo Alencar Mayke Bezerra Brazil São Paulo Crispim Marcell Croatia Zagreb Rubelj Ivica Czech Republic Prague Mracek Tomas Czech Republic Prague Elkalaf Moustafa Czech Republic Hradec Kralove Vernerova Andrea Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic Vestec Zobalova Renata Czech Republic Pilsen Markova Michaela Czech Republic Prague Hansikova Hana Czech Republic Prague Fisar Zdenek Czech Republic Pilsen Kuncova J Czech Republic Pilsen Kripnerova M Czech Republic Prague 4 Bajzikova Martina Czech Republic Prague Pecina Petr Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Hvidovre Nehlin Jan O Denmark Aarhus Rattan Suresh IS Denmark Copenhagen Rasmussen LJ Denmark Copenhagen Desler C Denmark Malov Hey-Mogensen M Denmark Copenhagen N Mortensen OH Egypt Cairo Ali Sameh S Egypt Cairo Abdel-Rahman Engy Ali Estonia Tallinn Truu Laura Estonia Tartu Grellier T Estonia Tallinn Klepinina Lyudmila Finland Turku Stier Antoine Finland Helsinki Pirinen E Finland Jyvaeskylae Lehti Maarit France Bron Rizwan Alam M France Paris Petit Patrice X France Bordeaux cedex Mourier A Germany Magdeburg Vieira Ligo Teixeira Camila Germany Mainz Kalinovic S Germany Freising Klingenspor Martin Germany Leipzig Ost Mario Germany Freising-Weihenstephan Musiol Eva Germany Regensburg Renner-Sattler Kathrin Germany Duesseldorf Piel Sarah Germany Freising Keppner Gloria Germany Ulm Hartmann C Germany Giessen Eckert Gunter P Germany Munich Jastroch M Germany Neuherberg Borchard Sabine Germany Hamburg Singer Dominique Germany Ulm Kolassa Iris Germany Freising Schoettl T Germany Gießen Silaidos Carmina Germany Düsseldorf Prigione Alessandro Germany Biberach an der Riß Nold V Germany Ulm Calzia Enrico Germany Freising Fromme Tobias Germany Duesseldorf Eckers A Germany Mainz Vujacic-Mirski Ksenija Germany Ulm Schaefer PM Greece Athens Trougakos Ioannis P Hungary Debrecen Verebne Tar K Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Ireland Dublin Porter Richard K Ireland Sligo Murphy J Ireland Dublin Karavyraki Marilena Israel Jerusalem Saada Reisch Ann Israel Zrifin Sova Marina Israel Rishon Le Zion Hachmo Yafit Italy Verona Calabria Elisa Italy Bologna Genova Maria Luisa Italy Padova Fernandez-Vizarra Erika Italy Bolzano Pichler Irene Italy Bolzano Castelo Rueda Maria Paulina Italy Bolzano/Bozen Volani Chiara Italy Bologna Paterlini S Italy Ancona Battino Maurizio Italy Bari Agrimi G Italy Bologna Porcelli AM Italy Milano Bottoni P Italy Rome Scatena Roberto Italy Bologna Gasparre G Japan Sapporo Shirakawa R Latvia Riga Makarova Elina Latvia Riga Kidere Dita Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Groningen Rots MG Netherlands Wageningen Keijer Jaap Netherlands Nijmegen Koopman Werner JH Norway Oslo Eide L Norway Oslo Nikolic N Norway Oslo Lund Jenny Norway Oslo Rustan AC Norway Bergen Tronstad Karl Johan Palestine Ramallah Stiban Johnny Poland Lodz Labieniec-Watala Magdalena Poland Warsaw Kampa Rafal Pawel Poland Lodz Siewiera Karolina Poland Warsaw Sek Aleksandra Poland Poznan Rybacka-Mossakowska Joanna Poland Warsaw Wasilewski M Poland Krakow Kaczara P Poland Poznan Michalak Slawomir Poland Warsaw Dymkowska Dorota Poland Poznań Ludziejewska A Poland Lodz Watala Cezary Poland Poznan Swiniuch Daria Portugal Porto Oliveira Pedro Fontes Portugal Covilhã Dias Tania R Portugal Coimbra Carvalho Eugenia Portugal Coimbra Pereira Susana P Portugal Porto Bernardino RL Portugal Porto Alves Marco G Portugal Cantanhede Pinho Sonia A Portugal Porto Beleza Jorge Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Porto Carrageta D Portugal Porto Magalhaes Jose Portugal Coimbra Palmeira Carlos Portugal Porto Martins Ana Dias Portugal Porto Crisostomo Luis Portugal Porto Moreira Bruno P Portugal Cantanhede Bento Guida Jose Freitas Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Korea Daejeon Park SH Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Lighezan R Romania Timisoara Danila Maria-Daniela Romania Timisoara Lelcu Theia Romania Timisoara Muntean Danina M Romania Timisoara Betiu AM Romania Timisoara Avram Vlad F Russia Moscow Itkis YS Russia Moscow Tsygankova P Russia Moscow Krylova T Saudi Arabia Giza Kotb IA Serbia Belgrade Ivanovic Tijana Serbia Belgrade Jeremic M Serbia Belgrade Isakovic A Serbia Belgrade Vukojevic M Serbia Belgrad Markovic Ivanka Serbia Belgrade Misirlic-Dencic S Slovakia Bratislava Maderova D Slovakia Bratislava Nemec Michal Slovakia Bratislava Ukropec J Slovakia Košice Cizmarova Beata Slovakia Bratislava Sumbalova Zuzana Slovakia Bratislava Ukropcova B Slovenia Ljubljana Pirkmajer Sergej Slovenia Ljubljana Mars T Slovenia Ljubljana Kopitar-Jerala Natasa Spain Barcelona Juarez D Spain A Coruña Mayan MD Spain Valencia Borras Consuelo Spain Lleida Nogueras L Spain Armilla, Granada Escames Germaine Spain Madrid De los Rios C Spain Barcelona Dahdah Norma Ramon Spain A Coruña Blanco FJ Spain Barcelona Gama Perez Pau Spain Valencia Fuertes Agudo Marina Spain Madrid Cano-Abad MF Spain Sevilla Muntane Jordi Spain Sevilla Bustos Matilde Spain Salamanca Almeida Angeles Spain Madrid Cadenas S Spain Barcelona Tura Ceide O Spain Madrid Monsalve Maria Spain Madrid Lopez MG Spain Barcelona Hyrossova Petra Spain Barcelona Arago Marc Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Barcelona Smolders V Spain Barcelona Gil J Spain Valencia Victor Victor Manuel Spain Ourense Reboredo-Rodriguez Patricia Spain Barcelona Gonzalez Casacuberta I Spain Valencia Casado Pinna Marta Spain Lleida Gonzalo Hugo Spain Barcelona Zorzano Antonio Spain Granada Quiles JL Sweden Linköping Fontes-Oliveira CC Sweden Lund Leffler M Sweden Lund Abliva Sweden Lund Chamkha Imen Sweden Södertälje Alfaro-Moreno E Sweden Lund Karlsson M Sweden Lund Westerlund E Sweden Lund Elmer Eskil Sweden Lund Simon Serrano Sonia Sweden, SE Lund Aasander Frostner Eleonor Switzerland Geneva Martinou JC Switzerland Lausanne Zanou Nadege The Netherlands BT Amsterdam Wuest Rob CI Turkey Istanbul Baykal AT Turkey Tekirdag Aral Cenk Turkey Izmir Aydin HH Turkey Ankara Engin Ayse Basak Turkey Ankara Tuncay Erkan USA Philadelphia Starr Jonathan USA Fayetteville Rao RR USA St Louis Welch R USA Philadelphia Janowska Joanna USA Philadelphia Kilbaugh Todd USA Orlando Coen Paul M USA Baton Rouge Irving Brian A USA Iowa City Buettner Garry R USA Fayetteville Iyer Shilpa United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Leicester Moisoi Nicoleta United Kingdom Brighton Moore Anthony L United Kingdom Birmingham Thakker A United Kingdom Birmingham Joanisse S United Kingdom Stoke-on-Trent McStay Gavin P Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.