Difference between revisions of "MitoEAGLE data: blood and cultured cells"
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== WG4 Participants == | == WG4 Participants == | ||
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Revision as of 14:58, 27 April 2016
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News and Events |  |
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Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Meszaros Andras T |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Arnould Thierry |
| Belgium | Namur | Renard P |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Copenhagen | Desler C |
| Denmark | Copenhagen | Rasmussen LJ |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Grellier T |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| France | Bron | Rizwan Alam M |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| Germany | Freising | Keppner Gloria |
| Germany | Giessen | Eckert Gunter P |
| Germany | Freising | Fromme Tobias |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Munich | Jastroch M |
| Germany | Hamburg | Singer Dominique |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Freising | Schoettl T |
| Germany | Biberach an der Riß | Nold V |
| Germany | Ulm | Calzia Enrico |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Ulm | Hartmann C |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Schaefer PM |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Freising | Klingenspor Martin |
| Germany | Ulm | Kolassa Iris |
| Germany | Leipzig | Ost Mario |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Ireland | Dublin | Porter Richard K |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Sligo | Murphy J |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Zrifin | Sova Marina |
| Israel | Jerusalem | Saada Reisch Ann |
| Italy | Bolzano | Pichler Irene |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Ancona | Battino Maurizio |
| Italy | Bari | Agrimi G |
| Italy | Bologna | Porcelli AM |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Milano | Bottoni P |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Rome | Scatena Roberto |
| Italy | Bologna | Gasparre G |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Groningen | Rots MG |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Poznań | Ludziejewska A |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznan | Swiniuch Daria |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Lodz | Watala Cezary |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Lodz | Siewiera Karolina |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Carrageta D |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Lighezan R |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Russia | Moscow | Itkis YS |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava | Nemec Michal |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Spain | Barcelona | Gil J |
| Spain | Madrid | Cano-Abad MF |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Madrid | Cadenas S |
| Spain | Madrid | Monsalve Maria |
| Spain | Madrid | Lopez MG |
| Spain | Lleida | Nogueras L |
| Spain | Valencia | Borras Consuelo |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Granada | Quiles JL |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Barcelona | Juarez D |
| Spain | Sevilla | Bustos Matilde |
| Spain | A Coruña | Mayan MD |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Madrid | De los Rios C |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Smolders V |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Abliva |
| Sweden | Lund | Elmer Eskil |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Chamkha Imen |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| USA | Iowa City | Buettner Garry R |
| USA | Fayetteville | Rao RR |
| USA | St Louis | Welch R |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Orlando | Coen Paul M |
| USA | Baton Rouge | Irving Brian A |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| USA | Philadelphia | Janowska Joanna |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Brighton | Moore Anthony L |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
