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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Gnaiger Erich |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Renard P |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Copenhagen | Desler C |
| Egypt | Cairo | Ali Sameh S |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Truu Laura |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| France | Paris | Petit Patrice X |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| Germany | Giessen | Eckert Gunter P |
| Germany | Freising | Fromme Tobias |
| Germany | Munich | Jastroch M |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Biberach an der Riß | Nold V |
| Germany | Hamburg | Singer Dominique |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Ulm | Calzia Enrico |
| Germany | Ulm | Schaefer PM |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Ulm | Hartmann C |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Freising | Klingenspor Martin |
| Germany | Ulm | Kolassa Iris |
| Germany | Leipzig | Ost Mario |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Freising | Keppner Gloria |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Dublin | Porter Richard K |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Bologna | Porcelli AM |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Gasparre G |
| Italy | Rome | Scatena Roberto |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Groningen | Rots MG |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Poznań | Ludziejewska A |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznan | Swiniuch Daria |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Watala Cezary |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Lodz | Siewiera Karolina |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Wasilewski M |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Lelcu Theia |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Russia | Moscow | Itkis YS |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava | Nemec Michal |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Barcelona | Gil J |
| Spain | Madrid | Lopez MG |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Madrid | Cadenas S |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Lleida | Nogueras L |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Valencia | Borras Consuelo |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Juarez D |
| Spain | Barcelona | Zorzano Antonio |
| Spain | A Coruña | Mayan MD |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | De los Rios C |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Barcelona | Tura Ceide O |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Madrid | Cano-Abad MF |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Smolders V |
| Spain | Madrid | Monsalve Maria |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Karlsson M |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Elmer Eskil |
| Sweden | Lund | Abliva |
| Sweden | Lund | Chamkha Imen |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| USA | Iowa City | Buettner Garry R |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Orlando | Coen Paul M |
| USA | Baton Rouge | Irving Brian A |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| USA | Philadelphia | Janowska Joanna |
| USA | St Louis | Welch R |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
