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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Fischer Christine |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Hoppel Florian |
Austria | Innsbruck | Torres-Quesada Omar |
Belgium | Namur | Arnould Thierry |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Detraux Damien |
Belgium | Namur | Renard P |
Belgium | Leuven | Bird Matthew |
Bosnia and Herzegovina | Tuzla | Jusic Amela |
Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
Brazil | Sao Paulo | Dominguez-Amorocho OA |
Brazil | Sao Paulo | Alencar Mayke Bezerra |
Brazil | São Paulo | Crispim Marcell |
Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Prague | Mracek Tomas |
Czech Republic | Pilsen | Kripnerova M |
Czech Republic | Vestec | Zobalova Renata |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic | Hradec Kralove | Vernerova Andrea |
Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
Czech Republic | Prague | Fisar Zdenek |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Prague 4 | Bajzikova Martina |
Czech Republic | Prague | Pecina Petr |
Czech Republic | Prague | Hansikova Hana |
Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus | Rattan Suresh IS |
Denmark | Malov | Hey-Mogensen M |
Denmark | Copenhagen | Desler C |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Rasmussen LJ |
Egypt | Cairo | Ali Sameh S |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Estonia | Tallinn | Truu Laura |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tartu | Grellier T |
Finland | Jyvaeskylae | Lehti Maarit |
Finland | Turku | Stier Antoine |
Finland | Helsinki | Pirinen E |
France | Bron | Rizwan Alam M |
France | Paris | Petit Patrice X |
France | Bordeaux cedex | Mourier A |
Germany | Freising | Klingenspor Martin |
Germany | Freising | Fromme Tobias |
Germany | Mainz | Vujacic-Mirski Ksenija |
Germany | Leipzig | Ost Mario |
Germany | Duesseldorf | Piel Sarah |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Freising | Keppner Gloria |
Germany | Mainz | Kalinovic S |
Germany | Magdeburg | Vieira Ligo Teixeira Camila |
Germany | Giessen | Eckert Gunter P |
Germany | Munich | Jastroch M |
Germany | Freising-Weihenstephan | Musiol Eva |
Germany | Biberach an der Riß | Nold V |
Germany | Hamburg | Singer Dominique |
Germany | Ulm | Hartmann C |
Germany | Freising | Schoettl T |
Germany | Duesseldorf | Eckers A |
Germany | Ulm | Schaefer PM |
Germany | Ulm | Calzia Enrico |
Germany | Neuherberg | Borchard Sabine |
Germany | Ulm | Kolassa Iris |
Germany | Gießen | Silaidos Carmina |
Germany | Düsseldorf | Prigione Alessandro |
Greece | Athens | Trougakos Ioannis P |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Komlodi Timea |
Ireland | Dublin | Karavyraki Marilena |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Israel | Rishon Le Zion | Hachmo Yafit |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Zrifin | Sova Marina |
Italy | Bolzano | Pichler Irene |
Italy | Bologna | Paterlini S |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Ancona | Battino Maurizio |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bologna | Porcelli AM |
Italy | Bolzano | Castelo Rueda Maria Paulina |
Italy | Rome | Scatena Roberto |
Italy | Bologna | Gasparre G |
Italy | Milano | Bottoni P |
Italy | Verona | Calabria Elisa |
Italy | Bologna | Genova Maria Luisa |
Japan | Sapporo | Shirakawa R |
Latvia | Riga | Makarova Elina |
Latvia | Riga | Kidere Dita |
Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
Malta | Msida | Vella Joanna |
Netherlands | Groningen | Rots MG |
Netherlands | Wageningen | Keijer Jaap |
Netherlands | Nijmegen | Koopman Werner JH |
Norway | Oslo | Eide L |
Norway | Oslo | Nikolic N |
Norway | Oslo | Rustan AC |
Norway | Bergen | Tronstad Karl Johan |
Norway | Oslo | Lund Jenny |
Palestine | Ramallah | Stiban Johnny |
Poland | Poznań | Ludziejewska A |
Poland | Lodz | Siewiera Karolina |
Poland | Warsaw | Wasilewski M |
Poland | Poznan | Swiniuch Daria |
Poland | Krakow | Kaczara P |
Poland | Poznan | Michalak Slawomir |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Dymkowska Dorota |
Poland | Warsaw | Sek Aleksandra |
Poland | Poznan | Rybacka-Mossakowska Joanna |
Poland | Lodz | Watala Cezary |
Poland | Lodz | Labieniec-Watala Magdalena |
Portugal | Porto | Crisostomo Luis |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Porto | Moreira Bruno P |
Portugal | Cantanhede | Bento Guida Jose Freitas |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Covilhã | Dias Tania R |
Portugal | Porto | Bernardino RL |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Pereira Susana P |
Portugal | Cantanhede | Pinho Sonia A |
Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
Portugal | Porto | Beleza Jorge |
Portugal | Porto | Carrageta D |
Portugal | Porto | Magalhaes Jose |
Portugal | Porto | Martins Ana Dias |
Republic of Korea | Daejeon | Park SH |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Serbia | Belgrade | Pavlovic Kasja |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Romania | Timisoara | Betiu AM |
Romania | Timisoara | Avram Vlad F |
Romania | Timisoara | Danila Maria-Daniela |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Lelcu Theia |
Romania | Timisoara | Lighezan R |
Russia | Moscow | Itkis YS |
Russia | Moscow | Tsygankova P |
Russia | Moscow | Krylova T |
Saudi Arabia | Giza | Kotb IA |
Serbia | Belgrad | Markovic Ivanka |
Serbia | Belgrade | Misirlic-Dencic S |
Serbia | Belgrade | Isakovic A |
Serbia | Belgrade | Ivanovic Tijana |
Serbia | Belgrade | Jeremic M |
Serbia | Belgrade | Vukojevic M |
Slovakia | Košice | Cizmarova Beata |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Ukropec J |
Slovakia | Bratislava | Maderova D |
Slovakia | Bratislava | Nemec Michal |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovenia | Ljubljana | Kopitar-Jerala Natasa |
Slovenia | Ljubljana | Pirkmajer Sergej |
Slovenia | Ljubljana | Mars T |
Spain | Valencia | Victor Victor Manuel |
Spain | Armilla, Granada | Escames Germaine |
Spain | Ourense | Reboredo-Rodriguez Patricia |
Spain | Madrid | Cano-Abad MF |
Spain | A Coruña | Blanco FJ |
Spain | Lleida | Gonzalo Hugo |
Spain | Madrid | Monsalve Maria |
Spain | Madrid | Lopez MG |
Spain | Valencia | Borras Consuelo |
Spain | Lleida | Nogueras L |
Spain | Barcelona | Dahdah Norma Ramon |
Spain | Madrid | Cadenas S |
Spain | Valencia | Fuertes Agudo Marina |
Spain | Sevilla | Muntane Jordi |
Spain | Barcelona | Gonzalez Casacuberta I |
Spain | Valencia | Casado Pinna Marta |
Spain | Granada | Quiles JL |
Spain | Sevilla | Bustos Matilde |
Spain | Salamanca | Almeida Angeles |
Spain | Barcelona | Tura Ceide O |
Spain | Barcelona | Juarez D |
Spain | Barcelona | Arago Marc |
Spain | A Coruña | Mayan MD |
Spain | Barcelona | Hyrossova Petra |
Spain | Barcelona | Zorzano Antonio |
Spain | Madrid | De los Rios C |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Barcelona | Smolders V |
Spain | Barcelona | Gama Perez Pau |
Spain | Barcelona | Gil J |
Sweden | Lund | Simon Serrano Sonia |
Sweden | Lund | Leffler M |
Sweden | Lund | Westerlund E |
Sweden | Lund | Abliva |
Sweden | Lund | Karlsson M |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden | Lund | Elmer Eskil |
Sweden | Lund | Chamkha Imen |
Sweden | Södertälje | Alfaro-Moreno E |
Sweden, SE | Lund | Aasander Frostner Eleonor |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Geneva | Martinou JC |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Ankara | Engin Ayse Basak |
Turkey | Ankara | Tuncay Erkan |
Turkey | Istanbul | Baykal AT |
Turkey | Tekirdag | Aral Cenk |
Turkey | Izmir | Aydin HH |
USA | Iowa City | Buettner Garry R |
USA | St Louis | Welch R |
USA | Fayetteville | Rao RR |
USA | Philadelphia | Kilbaugh Todd |
USA | Baton Rouge | Irving Brian A |
USA | Philadelphia | Starr Jonathan |
USA | Orlando | Coen Paul M |
USA | Fayetteville | Iyer Shilpa |
USA | Philadelphia | Janowska Joanna |
United Kingdom | Stoke-on-Trent | McStay Gavin P |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Leicester | Moisoi Nicoleta |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Birmingham | Joanisse S |
United Kingdom | Brighton | Moore Anthony L |
Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).