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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Arnould Thierry |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Renard P |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen | Desler C |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Malov | Hey-Mogensen M |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Grellier T |
| Finland | Helsinki | Pirinen E |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| France | Bordeaux cedex | Mourier A |
| France | Bron | Rizwan Alam M |
| France | Paris | Petit Patrice X |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Ulm | Kolassa Iris |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Freising | Klingenspor Martin |
| Germany | Freising | Fromme Tobias |
| Germany | Leipzig | Ost Mario |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Freising | Keppner Gloria |
| Germany | Giessen | Eckert Gunter P |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Munich | Jastroch M |
| Germany | Biberach an der Riß | Nold V |
| Germany | Hamburg | Singer Dominique |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Freising | Schoettl T |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Hartmann C |
| Germany | Ulm | Calzia Enrico |
| Germany | Ulm | Schaefer PM |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Dublin | Porter Richard K |
| Ireland | Sligo | Murphy J |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Bologna | Gasparre G |
| Italy | Rome | Scatena Roberto |
| Italy | Milano | Bottoni P |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Bologna | Porcelli AM |
| Italy | Ancona | Battino Maurizio |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Nijmegen | Koopman Werner JH |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Groningen | Rots MG |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Eide L |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Lodz | Watala Cezary |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Poznań | Ludziejewska A |
| Poland | Lodz | Siewiera Karolina |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Wasilewski M |
| Poland | Poznan | Swiniuch Daria |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Porto | Bernardino RL |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Beleza Jorge |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Covilhã | Dias Tania R |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Lelcu Theia |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Muntean Danina M |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Juarez D |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Barcelona | Tura Ceide O |
| Spain | Barcelona | Zorzano Antonio |
| Spain | A Coruña | Mayan MD |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Madrid | De los Rios C |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Smolders V |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Madrid | Cano-Abad MF |
| Spain | A Coruña | Blanco FJ |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Madrid | Monsalve Maria |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Madrid | Lopez MG |
| Spain | Madrid | Cadenas S |
| Spain | Valencia | Borras Consuelo |
| Spain | Lleida | Nogueras L |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Valencia | Casado Pinna Marta |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Elmer Eskil |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Abliva |
| Sweden | Lund | Karlsson M |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Geneva | Martinou JC |
| Switzerland | Lausanne | Zanou Nadege |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Janowska Joanna |
| USA | Iowa City | Buettner Garry R |
| USA | St Louis | Welch R |
| USA | Fayetteville | Rao RR |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Orlando | Coen Paul M |
| USA | Baton Rouge | Irving Brian A |
| USA | Philadelphia | Starr Jonathan |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).
- Specifically, we are studying mitochondrial metabolism in cancer cells. At this aim we are also participating to mt-HPP project, an Italian consortium whose target is a definition of mitochondrial proteome in health and disease. Briefly, my specific activity is to compare mitochondrial functional status with the mitochondrial proteome picture in different human cell lines. I do think that a "contact" between these two important initiatives should be useful for both. - Scatena Roberto (2016).
