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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Fischer Christine Austria Innsbruck Meszaros Andras T Austria Innsbruck Gnaiger Erich Austria Innsbruck Torres-Quesada Omar Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Doerrier Carolina Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Karabatsiakis Alexander Austria Innsbruck Hoppel Florian Belgium Leuven Bird Matthew Belgium Namur Arnould Thierry Belgium Liege Votion Dominique-Marie Belgium Namur Renard P Belgium Namur Detraux Damien Bosnia and Herzegovina Tuzla Jusic Amela Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil Sao Paulo Dominguez-Amorocho OA Brazil Sao Paulo Alencar Mayke Bezerra Brazil São Paulo Crispim Marcell Croatia Zagreb Rubelj Ivica Czech Republic Prague Mracek Tomas Czech Republic Vestec Zobalova Renata Czech Republic Prague Hansikova Hana Czech Republic Pilsen Markova Michaela Czech Republic Pilsen Kripnerova M Czech Republic Prague Fisar Zdenek Czech Republic Pilsen Kuncova J Czech Republic Prague Elkalaf Moustafa Czech Republic Prague Pecina Petr Czech Republic Hradec Kralove Vernerova Andrea Czech Republic Prague 4 Bajzikova Martina Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Copenhagen Desler C Denmark Malov Hey-Mogensen M Denmark Hvidovre Nehlin Jan O Denmark Aarhus Rattan Suresh IS Denmark Copenhagen Rasmussen LJ Denmark Copenhagen N Mortensen OH Egypt Cairo Abdel-Rahman Engy Ali Egypt Cairo Ali Sameh S Estonia Tallinn Truu Laura Estonia Tallinn Klepinina Lyudmila Estonia Tartu Grellier T Finland Jyvaeskylae Lehti Maarit Finland Turku Stier Antoine Finland Helsinki Pirinen E France Bordeaux cedex Mourier A France Paris Petit Patrice X France Bron Rizwan Alam M Germany Leipzig Ost Mario Germany Neuherberg Borchard Sabine Germany Duesseldorf Piel Sarah Germany Ulm Kolassa Iris Germany Regensburg Renner-Sattler Kathrin Germany Freising Keppner Gloria Germany Giessen Eckert Gunter P Germany Gießen Silaidos Carmina Germany Düsseldorf Prigione Alessandro Germany Freising Fromme Tobias Germany Munich Jastroch M Germany Hamburg Singer Dominique Germany Mainz Vujacic-Mirski Ksenija Germany Biberach an der Riß Nold V Germany Freising Schoettl T Germany Duesseldorf Eckers A Germany Ulm Calzia Enrico Germany Ulm Schaefer PM Germany Mainz Kalinovic S Germany Magdeburg Vieira Ligo Teixeira Camila Germany Freising-Weihenstephan Musiol Eva Germany Ulm Hartmann C Germany Freising Klingenspor Martin Greece Athens Trougakos Ioannis P Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Hungary Debrecen Verebne Tar K Ireland Dublin Porter Richard K Ireland Sligo Murphy J Ireland Dublin Karavyraki Marilena Israel Rishon Le Zion Hachmo Yafit Israel Zrifin Sova Marina Israel Jerusalem Saada Reisch Ann Italy Bolzano/Bozen Volani Chiara Italy Bologna Paterlini S Italy Bologna Porcelli AM Italy Ancona Battino Maurizio Italy Bologna Gasparre G Italy Rome Scatena Roberto Italy Milano Bottoni P Italy Verona Calabria Elisa Italy Padova Fernandez-Vizarra Erika Italy Bologna Genova Maria Luisa Italy Bolzano Castelo Rueda Maria Paulina Italy Bolzano Pichler Irene Japan Sapporo Shirakawa R Latvia Riga Makarova Elina Latvia Riga Kidere Dita Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Nijmegen Koopman Werner JH Netherlands Wageningen Keijer Jaap Netherlands Groningen Rots MG Norway Oslo Lund Jenny Norway Oslo Nikolic N Norway Oslo Rustan AC Norway Bergen Tronstad Karl Johan Norway Oslo Eide L Palestine Ramallah Stiban Johnny Poland Lodz Siewiera Karolina Poland Warsaw Wasilewski M Poland Krakow Kaczara P Poland Poznan Michalak Slawomir Poland Poznań Ludziejewska A Poland Warsaw Dymkowska Dorota Poland Poznan Swiniuch Daria Poland Warsaw Kampa Rafal Pawel Poland Lodz Watala Cezary Poland Warsaw Sek Aleksandra Poland Poznan Rybacka-Mossakowska Joanna Poland Lodz Labieniec-Watala Magdalena Portugal Cantanhede Pinho Sonia A Portugal Porto Beleza Jorge Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Porto Magalhaes Jose Portugal Porto Carrageta D Portugal Porto Martins Ana Dias Portugal Porto Crisostomo Luis Portugal Coimbra Palmeira Carlos Portugal Porto Moreira Bruno P Portugal Cantanhede Bento Guida Jose Freitas Portugal Porto Oliveira Pedro Fontes Portugal Covilhã Dias Tania R Portugal Coimbra Carvalho Eugenia Portugal Porto Bernardino RL Portugal Porto Alves Marco G Portugal Coimbra Pereira Susana P Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Korea Daejeon Park SH Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Betiu AM Romania Timisoara Avram Vlad F Romania Timisoara Muntean Danina M Romania Timisoara Lighezan R Romania Timisoara Danila Maria-Daniela Romania Timisoara Lelcu Theia Russia Moscow Itkis YS Russia Moscow Tsygankova P Russia Moscow Krylova T Saudi Arabia Giza Kotb IA Serbia Belgrad Markovic Ivanka Serbia Belgrade Misirlic-Dencic S Serbia Belgrade Ivanovic Tijana Serbia Belgrade Jeremic M Serbia Belgrade Isakovic A Serbia Belgrade Vukojevic M Slovakia Bratislava Ukropec J Slovakia Košice Cizmarova Beata Slovakia Bratislava Ukropcova B Slovakia Bratislava Maderova D Slovakia Bratislava Sumbalova Zuzana Slovakia Bratislava Nemec Michal Slovenia Ljubljana Kopitar-Jerala Natasa Slovenia Ljubljana Pirkmajer Sergej Slovenia Ljubljana Mars T Spain Salamanca Almeida Angeles Spain Barcelona Tura Ceide O Spain Madrid Cano-Abad MF Spain Sevilla Bustos Matilde Spain A Coruña Blanco FJ Spain Madrid Monsalve Maria Spain Barcelona Arago Marc Spain Barcelona Hyrossova Petra Spain Barcelona Smolders V Spain Madrid Lopez MG Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Barcelona Gil J Spain Valencia Victor Victor Manuel Spain Madrid Cadenas S Spain Ourense Reboredo-Rodriguez Patricia Spain Lleida Gonzalo Hugo Spain Barcelona Gonzalez Casacuberta I Spain Valencia Casado Pinna Marta Spain Lleida Nogueras L Spain Granada Quiles JL Spain Valencia Borras Consuelo Spain Barcelona Dahdah Norma Ramon Spain Barcelona Juarez D Spain Barcelona Zorzano Antonio Spain A Coruña Mayan MD Spain Valencia Fuertes Agudo Marina Spain Madrid De los Rios C Spain Sevilla Muntane Jordi Spain Barcelona Gama Perez Pau Spain Armilla, Granada Escames Germaine Sweden Lund Chamkha Imen Sweden Södertälje Alfaro-Moreno E Sweden Lund Westerlund E Sweden Lund Simon Serrano Sonia Sweden Lund Karlsson M Sweden Lund Leffler M Sweden Lund Elmer Eskil Sweden Linköping Fontes-Oliveira CC Sweden Lund Abliva Sweden, SE Lund Aasander Frostner Eleonor Switzerland Lausanne Zanou Nadege Switzerland Geneva Martinou JC The Netherlands BT Amsterdam Wuest Rob CI Turkey Ankara Engin Ayse Basak Turkey Ankara Tuncay Erkan Turkey Istanbul Baykal AT Turkey Tekirdag Aral Cenk Turkey Izmir Aydin HH USA Philadelphia Janowska Joanna USA St Louis Welch R USA Fayetteville Rao RR USA Iowa City Buettner Garry R USA Philadelphia Kilbaugh Todd USA Baton Rouge Irving Brian A USA Orlando Coen Paul M USA Fayetteville Iyer Shilpa USA Philadelphia Starr Jonathan United Kingdom Brighton Moore Anthony L United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Leicester Moisoi Nicoleta United Kingdom Birmingham Thakker A United Kingdom Stoke-on-Trent McStay Gavin P United Kingdom Birmingham Joanisse S Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.