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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Fischer Christine |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Gnaiger Erich |
| Belgium | Namur | Arnould Thierry |
| Belgium | Leuven | Bird Matthew |
| Belgium | Namur | Renard P |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Detraux Damien |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen | Desler C |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Aarhus | Rattan Suresh IS |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tartu | Grellier T |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tallinn | Truu Laura |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| France | Bordeaux cedex | Mourier A |
| France | Paris | Petit Patrice X |
| France | Bron | Rizwan Alam M |
| Germany | Hamburg | Singer Dominique |
| Germany | Ulm | Kolassa Iris |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Freising | Schoettl T |
| Germany | Ulm | Calzia Enrico |
| Germany | Biberach an der Riß | Nold V |
| Germany | Freising | Fromme Tobias |
| Germany | Duesseldorf | Eckers A |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Ulm | Schaefer PM |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Freising | Klingenspor Martin |
| Germany | Leipzig | Ost Mario |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Ulm | Hartmann C |
| Germany | Freising | Keppner Gloria |
| Germany | Giessen | Eckert Gunter P |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Munich | Jastroch M |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Tretter Laszlo |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Dublin | Porter Richard K |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Bologna | Paterlini S |
| Italy | Bologna | Porcelli AM |
| Italy | Ancona | Battino Maurizio |
| Italy | Bari | Agrimi G |
| Italy | Milano | Bottoni P |
| Italy | Bologna | Gasparre G |
| Italy | Rome | Scatena Roberto |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Nijmegen | Koopman Werner JH |
| Netherlands | Groningen | Rots MG |
| Netherlands | Wageningen | Keijer Jaap |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Oslo | Lund Jenny |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznań | Ludziejewska A |
| Poland | Lodz | Watala Cezary |
| Poland | Poznan | Swiniuch Daria |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Siewiera Karolina |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Portugal | Porto | Beleza Jorge |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Cantanhede | Pinho Sonia A |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lelcu Theia |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Russia | Moscow | Krylova T |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Madrid | Lopez MG |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Smolders V |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Juarez D |
| Spain | Lleida | Nogueras L |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | A Coruña | Mayan MD |
| Spain | Valencia | Borras Consuelo |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Madrid | De los Rios C |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | Cano-Abad MF |
| Spain | Madrid | Cadenas S |
| Spain | Barcelona | Tura Ceide O |
| Spain | Madrid | Monsalve Maria |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Elmer Eskil |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| Turkey | Ankara | Engin Ayse Basak |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Baton Rouge | Irving Brian A |
| USA | Orlando | Coen Paul M |
| USA | Iowa City | Buettner Garry R |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| USA | Fayetteville | Rao RR |
| USA | St Louis | Welch R |
| USA | Philadelphia | Janowska Joanna |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Brighton | Moore Anthony L |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
