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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country City Contact Armenia Yerevan Trchounian K Austria Innsbruck Meszaros Andras T Austria Innsbruck Gnaiger Erich Austria Innsbruck Torres-Quesada Omar Austria Innsbruck Garcia-Souza Luiz F Austria Innsbruck Doerrier Carolina Austria Innsbruck Cardoso Luiza HD Austria Innsbruck Karabatsiakis Alexander Austria Innsbruck Hoppel Florian Austria Innsbruck Fischer Christine Belgium Liege Votion Dominique-Marie Belgium Namur Arnould Thierry Belgium Namur Renard P Belgium Namur Detraux Damien Belgium Leuven Bird Matthew Bosnia and Herzegovina Tuzla Jusic Amela Bosnia and Herzegovina Tuzla Hajrulahovic Anesa Brazil Sao Paulo Verdaguer Ignasi Bofill Brazil Sao Paulo Dominguez-Amorocho OA Brazil Sao Paulo Alencar Mayke Bezerra Brazil São Paulo Crispim Marcell Croatia Zagreb Rubelj Ivica Czech Republic Prague Hansikova Hana Czech Republic Vestec Zobalova Renata Czech Republic Pilsen Kripnerova M Czech Republic Pilsen Markova Michaela Czech Republic Prague Fisar Zdenek Czech Republic Prague Elkalaf Moustafa Czech Republic Pilsen Kuncova J Czech Republic Hradec Kralove Vernerova Andrea Czech Republic Prague Pecina Petr Czech Republic Hradec Kralove Maseko Tumisang Edward Czech Republic Prague 4 Bajzikova Martina Czech Republic Prague Mracek Tomas Czech Republic and Australia Prague and Southport Neuzil Jiri Czech rep Hradec Kralove Falaye T Denmark Hvidovre Nehlin Jan O Denmark Malov Hey-Mogensen M Denmark Aarhus Rattan Suresh IS Denmark Copenhagen N Mortensen OH Denmark Copenhagen Rasmussen LJ Denmark Copenhagen Desler C Egypt Cairo Abdel-Rahman Engy Ali Egypt Cairo Ali Sameh S Estonia Tallinn Truu Laura Estonia Tallinn Klepinina Lyudmila Estonia Tartu Grellier T Finland Helsinki Pirinen E Finland Jyvaeskylae Lehti Maarit Finland Turku Stier Antoine France Bron Rizwan Alam M France Paris Petit Patrice X France Bordeaux cedex Mourier A Germany Regensburg Renner-Sattler Kathrin Germany Duesseldorf Piel Sarah Germany Freising Keppner Gloria Germany Gießen Silaidos Carmina Germany Düsseldorf Prigione Alessandro Germany Giessen Eckert Gunter P Germany Freising Fromme Tobias Germany Munich Jastroch M Germany Mainz Vujacic-Mirski Ksenija Germany Hamburg Singer Dominique Germany Freising Schoettl T Germany Mainz Kalinovic S Germany Magdeburg Vieira Ligo Teixeira Camila Germany Biberach an der Riß Nold V Germany Ulm Calzia Enrico Germany Duesseldorf Eckers A Germany Freising-Weihenstephan Musiol Eva Germany Ulm Schaefer PM Germany Ulm Hartmann C Germany Freising Klingenspor Martin Germany Neuherberg Borchard Sabine Germany Ulm Kolassa Iris Germany Leipzig Ost Mario Greece Athens Trougakos Ioannis P Hungary Debrecen Verebne Tar K Hungary Budapest Tretter Laszlo Hungary Budapest Komlodi Timea Ireland Dublin Porter Richard K Ireland Dublin Karavyraki Marilena Ireland Sligo Murphy J Israel Rishon Le Zion Hachmo Yafit Israel Jerusalem Saada Reisch Ann Israel Zrifin Sova Marina Italy Bologna Genova Maria Luisa Italy Bolzano Pichler Irene Italy Bolzano/Bozen Volani Chiara Italy Bologna Paterlini S Italy Bari Agrimi G Italy Bologna Porcelli AM Italy Ancona Battino Maurizio Italy Milano Bottoni P Italy Padova Fernandez-Vizarra Erika Italy Bolzano Castelo Rueda Maria Paulina Italy Bologna Gasparre G Italy Rome Scatena Roberto Italy Verona Calabria Elisa Japan Sapporo Shirakawa R Latvia Riga Makarova Elina Latvia Riga Kidere Dita Malaysia Kuala Lumpur Abdul Karim Norwahidah Malta Msida Vella Joanna Netherlands Wageningen Keijer Jaap Netherlands Groningen Rots MG Netherlands Nijmegen Koopman Werner JH Norway Oslo Lund Jenny Norway Oslo Nikolic N Norway Oslo Rustan AC Norway Bergen Tronstad Karl Johan Norway Oslo Eide L Palestine Ramallah Stiban Johnny Poland Lodz Siewiera Karolina Poland Poznań Ludziejewska A Poland Krakow Kaczara P Poland Poznan Michalak Slawomir Poland Warsaw Wasilewski M Poland Warsaw Dymkowska Dorota Poland Poznan Swiniuch Daria Poland Warsaw Kampa Rafal Pawel Poland Warsaw Sek Aleksandra Poland Lodz Watala Cezary Poland Poznan Rybacka-Mossakowska Joanna Poland Lodz Labieniec-Watala Magdalena Portugal Porto Carrageta D Portugal Coimbra Carvalho Eugenia Portugal Porto Magalhaes Jose Portugal Porto Martins Ana Dias Portugal Porto Crisostomo Luis Portugal Porto Moreira Bruno P Portugal Cantanhede Bento Guida Jose Freitas Portugal Porto Oliveira Pedro Fontes Portugal Coimbra Palmeira Carlos Portugal Covilhã Dias Tania R Portugal Porto Bernardino RL Portugal Porto Alves Marco G Portugal Coimbra Pereira Susana P Portugal Cantanhede Pinho Sonia A Portugal Catanhede Pereira da Silva Grilo da Silva Filomena Portugal Porto Beleza Jorge Republic of Korea Yuseong-gu Daejeon Quang Don T Republic of Korea Daejeon Park SH Republic of Serbia Belgrade Pavlovic Kasja Republic of Serbia Belgrade Krako Jakovljevic Nina Romania Timisoara Betiu AM Romania Timisoara Avram Vlad F Romania Timisoara Muntean Danina M Romania Timisoara Danila Maria-Daniela Romania Timisoara Lelcu Theia Romania Timisoara Lighezan R Russia Moscow Tsygankova P Russia Moscow Krylova T Russia Moscow Itkis YS Saudi Arabia Giza Kotb IA Serbia Belgrad Markovic Ivanka Serbia Belgrade Misirlic-Dencic S Serbia Belgrade Isakovic A Serbia Belgrade Ivanovic Tijana Serbia Belgrade Jeremic M Serbia Belgrade Vukojevic M Slovakia Košice Cizmarova Beata Slovakia Bratislava Ukropcova B Slovakia Bratislava Ukropec J Slovakia Bratislava Maderova D Slovakia Bratislava Sumbalova Zuzana Slovakia Bratislava Nemec Michal Slovenia Ljubljana Mars T Slovenia Ljubljana Kopitar-Jerala Natasa Slovenia Ljubljana Pirkmajer Sergej Spain Barcelona Arago Marc Spain A Coruña Blanco FJ Spain Barcelona Gama Perez Pau Spain Barcelona Hyrossova Petra Spain Armilla (Granada) Fernandez-Ortiz Marisol Spain Barcelona Smolders V Spain Madrid Cano-Abad MF Spain Barcelona Gil J Spain Valencia Victor Victor Manuel Spain Ourense Reboredo-Rodriguez Patricia Spain Madrid Cadenas S Spain Madrid Monsalve Maria Spain Madrid Lopez MG Spain Lleida Gonzalo Hugo Spain Valencia Borras Consuelo Spain Lleida Nogueras L Spain Barcelona Dahdah Norma Ramon Spain Barcelona Gonzalez Casacuberta I Spain Valencia Casado Pinna Marta Spain Barcelona Zorzano Antonio Spain Valencia Fuertes Agudo Marina Spain Sevilla Muntane Jordi Spain Granada Quiles JL Spain Barcelona Juarez D Spain Sevilla Bustos Matilde Spain Armilla, Granada Escames Germaine Spain Salamanca Almeida Angeles Spain A Coruña Mayan MD Spain Barcelona Tura Ceide O Spain Madrid De los Rios C Sweden Lund Chamkha Imen Sweden Södertälje Alfaro-Moreno E Sweden Lund Simon Serrano Sonia Sweden Lund Karlsson M Sweden Lund Westerlund E Sweden Lund Leffler M Sweden Lund Abliva Sweden Lund Elmer Eskil Sweden Linköping Fontes-Oliveira CC Sweden, SE Lund Aasander Frostner Eleonor Switzerland Lausanne Zanou Nadege Switzerland Geneva Martinou JC The Netherlands BT Amsterdam Wuest Rob CI Turkey Ankara Engin Ayse Basak Turkey Ankara Tuncay Erkan Turkey Istanbul Baykal AT Turkey Tekirdag Aral Cenk Turkey Izmir Aydin HH USA Iowa City Buettner Garry R USA Fayetteville Rao RR USA St Louis Welch R USA Philadelphia Kilbaugh Todd USA Orlando Coen Paul M USA Baton Rouge Irving Brian A USA Fayetteville Iyer Shilpa USA Philadelphia Starr Jonathan USA Philadelphia Janowska Joanna United Kingdom Oxford Makrecka-Kuka Marina United Kingdom Leicester Moisoi Nicoleta United Kingdom Stoke-on-Trent McStay Gavin P United Kingdom Birmingham Thakker A United Kingdom Birmingham Joanisse S United Kingdom Brighton Moore Anthony L Uruguay Montevideo Radi R
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
