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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Torres-Quesada Omar |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Hoppel Florian |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Fischer Christine |
| Belgium | Liege | Votion Dominique-Marie |
| Belgium | Namur | Arnould Thierry |
| Belgium | Namur | Renard P |
| Belgium | Namur | Detraux Damien |
| Belgium | Leuven | Bird Matthew |
| Bosnia and Herzegovina | Tuzla | Jusic Amela |
| Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
| Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
| Brazil | Sao Paulo | Dominguez-Amorocho OA |
| Brazil | Sao Paulo | Alencar Mayke Bezerra |
| Brazil | São Paulo | Crispim Marcell |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Vestec | Zobalova Renata |
| Czech Republic | Prague | Hansikova Hana |
| Czech Republic | Pilsen | Markova Michaela |
| Czech Republic | Pilsen | Kripnerova M |
| Czech Republic | Prague | Fisar Zdenek |
| Czech Republic | Pilsen | Kuncova J |
| Czech Republic | Prague 4 | Bajzikova Martina |
| Czech Republic | Prague | Pecina Petr |
| Czech Republic | Prague | Elkalaf Moustafa |
| Czech Republic | Hradec Kralove | Vernerova Andrea |
| Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
| Czech Republic | Prague | Mracek Tomas |
| Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Malov | Hey-Mogensen M |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Aarhus | Rattan Suresh IS |
| Denmark | Copenhagen | Rasmussen LJ |
| Denmark | Copenhagen | Desler C |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Grellier T |
| Finland | Jyvaeskylae | Lehti Maarit |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Pirinen E |
| France | Bordeaux cedex | Mourier A |
| France | Paris | Petit Patrice X |
| France | Bron | Rizwan Alam M |
| Germany | Gießen | Silaidos Carmina |
| Germany | Düsseldorf | Prigione Alessandro |
| Germany | Munich | Jastroch M |
| Germany | Hamburg | Singer Dominique |
| Germany | Freising | Fromme Tobias |
| Germany | Mainz | Vujacic-Mirski Ksenija |
| Germany | Freising | Schoettl T |
| Germany | Biberach an der Riß | Nold V |
| Germany | Ulm | Calzia Enrico |
| Germany | Mainz | Kalinovic S |
| Germany | Magdeburg | Vieira Ligo Teixeira Camila |
| Germany | Duesseldorf | Eckers A |
| Germany | Ulm | Schaefer PM |
| Germany | Freising-Weihenstephan | Musiol Eva |
| Germany | Freising | Klingenspor Martin |
| Germany | Ulm | Hartmann C |
| Germany | Leipzig | Ost Mario |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Neuherberg | Borchard Sabine |
| Germany | Freising | Keppner Gloria |
| Germany | Ulm | Kolassa Iris |
| Germany | Giessen | Eckert Gunter P |
| Greece | Athens | Trougakos Ioannis P |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Debrecen | Verebne Tar K |
| Ireland | Sligo | Murphy J |
| Ireland | Dublin | Karavyraki Marilena |
| Ireland | Dublin | Porter Richard K |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Zrifin | Sova Marina |
| Italy | Bologna | Paterlini S |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Ancona | Battino Maurizio |
| Italy | Bari | Agrimi G |
| Italy | Bologna | Porcelli AM |
| Italy | Milano | Bottoni P |
| Italy | Rome | Scatena Roberto |
| Italy | Bologna | Gasparre G |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bolzano | Castelo Rueda Maria Paulina |
| Italy | Verona | Calabria Elisa |
| Italy | Bologna | Genova Maria Luisa |
| Italy | Bolzano | Pichler Irene |
| Japan | Sapporo | Shirakawa R |
| Latvia | Riga | Makarova Elina |
| Latvia | Riga | Kidere Dita |
| Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
| Malta | Msida | Vella Joanna |
| Netherlands | Wageningen | Keijer Jaap |
| Netherlands | Groningen | Rots MG |
| Netherlands | Nijmegen | Koopman Werner JH |
| Norway | Oslo | Lund Jenny |
| Norway | Oslo | Nikolic N |
| Norway | Oslo | Rustan AC |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Oslo | Eide L |
| Palestine | Ramallah | Stiban Johnny |
| Poland | Warsaw | Wasilewski M |
| Poland | Krakow | Kaczara P |
| Poland | Poznan | Michalak Slawomir |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznań | Ludziejewska A |
| Poland | Poznan | Swiniuch Daria |
| Poland | Lodz | Watala Cezary |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Poznan | Rybacka-Mossakowska Joanna |
| Poland | Lodz | Siewiera Karolina |
| Portugal | Porto | Carrageta D |
| Portugal | Porto | Magalhaes Jose |
| Portugal | Porto | Martins Ana Dias |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Cantanhede | Bento Guida Jose Freitas |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Covilhã | Dias Tania R |
| Portugal | Porto | Bernardino RL |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Pereira Susana P |
| Portugal | Cantanhede | Pinho Sonia A |
| Portugal | Coimbra | Carvalho Eugenia |
| Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
| Portugal | Porto | Beleza Jorge |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Serbia | Belgrade | Pavlovic Kasja |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Romania | Timisoara | Betiu AM |
| Romania | Timisoara | Muntean Danina M |
| Romania | Timisoara | Avram Vlad F |
| Romania | Timisoara | Danila Maria-Daniela |
| Romania | Timisoara | Lighezan R |
| Romania | Timisoara | Lelcu Theia |
| Russia | Moscow | Krylova T |
| Russia | Moscow | Itkis YS |
| Russia | Moscow | Tsygankova P |
| Saudi Arabia | Giza | Kotb IA |
| Serbia | Belgrad | Markovic Ivanka |
| Serbia | Belgrade | Misirlic-Dencic S |
| Serbia | Belgrade | Isakovic A |
| Serbia | Belgrade | Ivanovic Tijana |
| Serbia | Belgrade | Jeremic M |
| Serbia | Belgrade | Vukojevic M |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Ukropec J |
| Slovakia | Bratislava | Ukropcova B |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava | Maderova D |
| Slovakia | Bratislava | Nemec Michal |
| Slovenia | Ljubljana | Pirkmajer Sergej |
| Slovenia | Ljubljana | Mars T |
| Slovenia | Ljubljana | Kopitar-Jerala Natasa |
| Spain | Barcelona | Arago Marc |
| Spain | Barcelona | Hyrossova Petra |
| Spain | Barcelona | Smolders V |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Madrid | Cadenas S |
| Spain | Madrid | Monsalve Maria |
| Spain | Madrid | Lopez MG |
| Spain | Barcelona | Gil J |
| Spain | Valencia | Victor Victor Manuel |
| Spain | Ourense | Reboredo-Rodriguez Patricia |
| Spain | Lleida | Gonzalo Hugo |
| Spain | Barcelona | Gonzalez Casacuberta I |
| Spain | Valencia | Borras Consuelo |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Lleida | Nogueras L |
| Spain | Barcelona | Dahdah Norma Ramon |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Juarez D |
| Spain | A Coruña | Mayan MD |
| Spain | Valencia | Fuertes Agudo Marina |
| Spain | Armilla, Granada | Escames Germaine |
| Spain | Sevilla | Muntane Jordi |
| Spain | Madrid | De los Rios C |
| Spain | A Coruña | Blanco FJ |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Barcelona | Tura Ceide O |
| Spain | Sevilla | Bustos Matilde |
| Spain | Salamanca | Almeida Angeles |
| Spain | Madrid | Cano-Abad MF |
| Sweden | Lund | Chamkha Imen |
| Sweden | Södertälje | Alfaro-Moreno E |
| Sweden | Lund | Karlsson M |
| Sweden | Lund | Westerlund E |
| Sweden | Lund | Simon Serrano Sonia |
| Sweden | Lund | Elmer Eskil |
| Sweden | Lund | Leffler M |
| Sweden | Lund | Abliva |
| Sweden | Linköping | Fontes-Oliveira CC |
| Sweden, SE | Lund | Aasander Frostner Eleonor |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Geneva | Martinou JC |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| Turkey | Ankara | Engin Ayse Basak |
| Turkey | Ankara | Tuncay Erkan |
| Turkey | Istanbul | Baykal AT |
| Turkey | Tekirdag | Aral Cenk |
| Turkey | Izmir | Aydin HH |
| USA | Fayetteville | Rao RR |
| USA | St Louis | Welch R |
| USA | Philadelphia | Kilbaugh Todd |
| USA | Iowa City | Buettner Garry R |
| USA | Orlando | Coen Paul M |
| USA | Baton Rouge | Irving Brian A |
| USA | Fayetteville | Iyer Shilpa |
| USA | Philadelphia | Starr Jonathan |
| USA | Philadelphia | Janowska Joanna |
| United Kingdom | Leicester | Moisoi Nicoleta |
| United Kingdom | Birmingham | Thakker A |
| United Kingdom | Stoke-on-Trent | McStay Gavin P |
| United Kingdom | Birmingham | Joanisse S |
| United Kingdom | Brighton | Moore Anthony L |
| United Kingdom | Oxford | Makrecka-Kuka Marina |
| Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
