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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE data: blood and cultured cells
WG4
- MITOEAGLE data repository for blood cells and clutured cells
WG4 Description
- Biopsy sampling of human muscle and fat tissue for mitochondrial studies is an invasive approach. More recently measurement of mitochondrial function in human blood cells – as well as temporary cryopreservation (Karabatsiakis 2014 Transl Psychiatry) – has become an attractive and by far less invasive alternative for mitochondrial diagnostics. Mitochondrial function of blood cells was linked with numerous diseases and metabolic conditions such as sepsis, neurodegenerative diseases, depression, hypertension, amyotrophic lateral sclerosis, and early-stage heart failure (e.g. Sjoevall 2013 Crit Care; Karabatsiakis 2014 Transl Psychiatry; Hagl 2014 J Alzheimer Dis). The use of human blood cells will thus predictably widen the applicability of respirometry in human physiology and will provide a significant stimulus for the entire field of mitochondrial medicine.
- A key towards achieving comparability among data sets and the applicability of human blood cells for the evaluation of mitochondrial fitness in health and disease is the standardization of the procedures to separate or isolate blood cells, the experimental procedure for the evaluation of mitochondrial respiratory characteristics, and the format for reporting. A consensus on protocols should be reached in WG4, calling for a Workshop right at the start of the COST Action. Depending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2).
WG4 Participants
Country | City | Contact |
---|---|---|
Armenia | Yerevan | Trchounian K |
Austria | Innsbruck | Gnaiger Erich |
Austria | Innsbruck | Cardoso Luiza HD |
Austria | Innsbruck | Garcia-Souza Luiz F |
Austria | Innsbruck | Doerrier Carolina |
Austria | Innsbruck | Fischer Christine |
Austria | Innsbruck | Meszaros Andras T |
Austria | Innsbruck | Karabatsiakis Alexander |
Austria | Innsbruck | Torres-Quesada Omar |
Austria | Innsbruck | Hoppel Florian |
Belgium | Liege | Votion Dominique-Marie |
Belgium | Namur | Arnould Thierry |
Belgium | Namur | Detraux Damien |
Belgium | Leuven | Bird Matthew |
Belgium | Namur | Renard P |
Bosnia and Herzegovina | Tuzla | Jusic Amela |
Bosnia and Herzegovina | Tuzla | Hajrulahovic Anesa |
Brazil | São Paulo | Crispim Marcell |
Brazil | Sao Paulo | Verdaguer Ignasi Bofill |
Brazil | Sao Paulo | Dominguez-Amorocho OA |
Brazil | Sao Paulo | Alencar Mayke Bezerra |
Croatia | Zagreb | Rubelj Ivica |
Czech Republic | Vestec | Zobalova Renata |
Czech Republic | Prague | Elkalaf Moustafa |
Czech Republic | Hradec Kralove | Vernerova Andrea |
Czech Republic | Pilsen | Markova Michaela |
Czech Republic | Hradec Kralove | Maseko Tumisang Edward |
Czech Republic | Prague | Fisar Zdenek |
Czech Republic | Pilsen | Kuncova J |
Czech Republic | Prague 4 | Bajzikova Martina |
Czech Republic | Prague | Hansikova Hana |
Czech Republic | Prague | Pecina Petr |
Czech Republic | Pilsen | Kripnerova M |
Czech Republic | Prague | Mracek Tomas |
Czech Republic and Australia | Prague and Southport | Neuzil Jiri |
Czech rep | Hradec Kralove | Falaye T |
Denmark | Hvidovre | Nehlin Jan O |
Denmark | Aarhus | Rattan Suresh IS |
Denmark | Malov | Hey-Mogensen M |
Denmark | Copenhagen | Desler C |
Denmark | Copenhagen N | Mortensen OH |
Denmark | Copenhagen | Rasmussen LJ |
Egypt | Cairo | Ali Sameh S |
Egypt | Cairo | Abdel-Rahman Engy Ali |
Estonia | Tallinn | Klepinina Lyudmila |
Estonia | Tallinn | Truu Laura |
Estonia | Tartu | Grellier T |
Finland | Helsinki | Pirinen E |
Finland | Turku | Stier Antoine |
Finland | Jyvaeskylae | Lehti Maarit |
France | Bron | Rizwan Alam M |
France | Paris | Petit Patrice X |
France | Bordeaux cedex | Mourier A |
Germany | Regensburg | Renner-Sattler Kathrin |
Germany | Duesseldorf | Piel Sarah |
Germany | Mainz | Kalinovic S |
Germany | Freising | Keppner Gloria |
Germany | Magdeburg | Vieira Ligo Teixeira Camila |
Germany | Giessen | Eckert Gunter P |
Germany | Munich | Jastroch M |
Germany | Freising-Weihenstephan | Musiol Eva |
Germany | Hamburg | Singer Dominique |
Germany | Ulm | Hartmann C |
Germany | Freising | Schoettl T |
Germany | Neuherberg | Borchard Sabine |
Germany | Biberach an der Riß | Nold V |
Germany | Ulm | Calzia Enrico |
Germany | Ulm | Kolassa Iris |
Germany | Duesseldorf | Eckers A |
Germany | Ulm | Schaefer PM |
Germany | Gießen | Silaidos Carmina |
Germany | Düsseldorf | Prigione Alessandro |
Germany | Freising | Fromme Tobias |
Germany | Mainz | Vujacic-Mirski Ksenija |
Germany | Freising | Klingenspor Martin |
Germany | Leipzig | Ost Mario |
Greece | Athens | Trougakos Ioannis P |
Hungary | Debrecen | Verebne Tar K |
Hungary | Budapest | Tretter Laszlo |
Hungary | Budapest | Komlodi Timea |
Ireland | Dublin | Porter Richard K |
Ireland | Sligo | Murphy J |
Ireland | Dublin | Karavyraki Marilena |
Israel | Jerusalem | Saada Reisch Ann |
Israel | Zrifin | Sova Marina |
Israel | Rishon Le Zion | Hachmo Yafit |
Italy | Bologna | Genova Maria Luisa |
Italy | Bolzano | Pichler Irene |
Italy | Padova | Fernandez-Vizarra Erika |
Italy | Bologna | Paterlini S |
Italy | Bolzano | Castelo Rueda Maria Paulina |
Italy | Bolzano/Bozen | Volani Chiara |
Italy | Ancona | Battino Maurizio |
Italy | Bari | Agrimi G |
Italy | Bologna | Porcelli AM |
Italy | Milano | Bottoni P |
Italy | Rome | Scatena Roberto |
Italy | Bologna | Gasparre G |
Italy | Verona | Calabria Elisa |
Japan | Sapporo | Shirakawa R |
Latvia | Riga | Makarova Elina |
Latvia | Riga | Kidere Dita |
Malaysia | Kuala Lumpur | Abdul Karim Norwahidah |
Malta | Msida | Vella Joanna |
Netherlands | Groningen | Rots MG |
Netherlands | Wageningen | Keijer Jaap |
Netherlands | Nijmegen | Koopman Werner JH |
Norway | Oslo | Nikolic N |
Norway | Oslo | Rustan AC |
Norway | Bergen | Tronstad Karl Johan |
Norway | Oslo | Lund Jenny |
Norway | Oslo | Eide L |
Palestine | Ramallah | Stiban Johnny |
Poland | Lodz | Siewiera Karolina |
Poland | Poznan | Swiniuch Daria |
Poland | Warsaw | Kampa Rafal Pawel |
Poland | Warsaw | Wasilewski M |
Poland | Krakow | Kaczara P |
Poland | Poznan | Michalak Slawomir |
Poland | Warsaw | Sek Aleksandra |
Poland | Warsaw | Dymkowska Dorota |
Poland | Poznan | Rybacka-Mossakowska Joanna |
Poland | Lodz | Watala Cezary |
Poland | Lodz | Labieniec-Watala Magdalena |
Poland | Poznań | Ludziejewska A |
Portugal | Cantanhede | Bento Guida Jose Freitas |
Portugal | Porto | Oliveira Pedro Fontes |
Portugal | Coimbra | Carvalho Eugenia |
Portugal | Covilhã | Dias Tania R |
Portugal | Porto | Bernardino RL |
Portugal | Porto | Alves Marco G |
Portugal | Coimbra | Pereira Susana P |
Portugal | Coimbra | Palmeira Carlos |
Portugal | Cantanhede | Pinho Sonia A |
Portugal | Catanhede | Pereira da Silva Grilo da Silva Filomena |
Portugal | Porto | Beleza Jorge |
Portugal | Porto | Carrageta D |
Portugal | Porto | Magalhaes Jose |
Portugal | Porto | Martins Ana Dias |
Portugal | Porto | Crisostomo Luis |
Portugal | Porto | Moreira Bruno P |
Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
Republic of Korea | Daejeon | Park SH |
Republic of Serbia | Belgrade | Pavlovic Kasja |
Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
Romania | Timisoara | Danila Maria-Daniela |
Romania | Timisoara | Lelcu Theia |
Romania | Timisoara | Muntean Danina M |
Romania | Timisoara | Betiu AM |
Romania | Timisoara | Lighezan R |
Romania | Timisoara | Avram Vlad F |
Russia | Moscow | Tsygankova P |
Russia | Moscow | Krylova T |
Russia | Moscow | Itkis YS |
Saudi Arabia | Giza | Kotb IA |
Serbia | Belgrade | Misirlic-Dencic S |
Serbia | Belgrade | Isakovic A |
Serbia | Belgrade | Ivanovic Tijana |
Serbia | Belgrade | Jeremic M |
Serbia | Belgrade | Vukojevic M |
Serbia | Belgrad | Markovic Ivanka |
Slovakia | Bratislava | Ukropcova B |
Slovakia | Bratislava | Maderova D |
Slovakia | Bratislava | Nemec Michal |
Slovakia | Bratislava | Ukropec J |
Slovakia | Bratislava | Sumbalova Zuzana |
Slovakia | Košice | Cizmarova Beata |
Slovenia | Ljubljana | Mars T |
Slovenia | Ljubljana | Kopitar-Jerala Natasa |
Slovenia | Ljubljana | Pirkmajer Sergej |
Spain | A Coruña | Blanco FJ |
Spain | Barcelona | Gama Perez Pau |
Spain | Madrid | Cano-Abad MF |
Spain | Valencia | Borras Consuelo |
Spain | Lleida | Nogueras L |
Spain | Barcelona | Dahdah Norma Ramon |
Spain | Madrid | Cadenas S |
Spain | Madrid | Monsalve Maria |
Spain | Madrid | Lopez MG |
Spain | Valencia | Fuertes Agudo Marina |
Spain | Sevilla | Muntane Jordi |
Spain | Barcelona | Tura Ceide O |
Spain | Sevilla | Bustos Matilde |
Spain | Salamanca | Almeida Angeles |
Spain | Barcelona | Arago Marc |
Spain | Barcelona | Gonzalez Casacuberta I |
Spain | Barcelona | Hyrossova Petra |
Spain | Barcelona | Smolders V |
Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
Spain | Valencia | Casado Pinna Marta |
Spain | Barcelona | Zorzano Antonio |
Spain | Granada | Quiles JL |
Spain | Barcelona | Gil J |
Spain | Valencia | Victor Victor Manuel |
Spain | Barcelona | Juarez D |
Spain | A Coruña | Mayan MD |
Spain | Ourense | Reboredo-Rodriguez Patricia |
Spain | Armilla, Granada | Escames Germaine |
Spain | Madrid | De los Rios C |
Spain | Lleida | Gonzalo Hugo |
Sweden | Lund | Simon Serrano Sonia |
Sweden | Lund | Leffler M |
Sweden | Lund | Abliva |
Sweden | Lund | Karlsson M |
Sweden | Lund | Westerlund E |
Sweden | Lund | Chamkha Imen |
Sweden | Lund | Elmer Eskil |
Sweden | Södertälje | Alfaro-Moreno E |
Sweden | Linköping | Fontes-Oliveira CC |
Sweden, SE | Lund | Aasander Frostner Eleonor |
Switzerland | Lausanne | Zanou Nadege |
Switzerland | Geneva | Martinou JC |
The Netherlands | BT Amsterdam | Wuest Rob CI |
Turkey | Ankara | Tuncay Erkan |
Turkey | Istanbul | Baykal AT |
Turkey | Ankara | Engin Ayse Basak |
Turkey | Tekirdag | Aral Cenk |
Turkey | Izmir | Aydin HH |
USA | Fayetteville | Rao RR |
USA | St Louis | Welch R |
USA | Philadelphia | Kilbaugh Todd |
USA | Philadelphia | Starr Jonathan |
USA | Orlando | Coen Paul M |
USA | Baton Rouge | Irving Brian A |
USA | Philadelphia | Janowska Joanna |
USA | Fayetteville | Iyer Shilpa |
USA | Iowa City | Buettner Garry R |
United Kingdom | Oxford | Makrecka-Kuka Marina |
United Kingdom | Leicester | Moisoi Nicoleta |
United Kingdom | Birmingham | Thakker A |
United Kingdom | Brighton | Moore Anthony L |
United Kingdom | Birmingham | Joanisse S |
United Kingdom | Stoke-on-Trent | McStay Gavin P |
Uruguay | Montevideo | Radi R |
WG4 Management
Tasks
- Kick-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.
- Development of SOPs for cell separation.
- Development of laboratory protocols for individual blood cell types (and other cell types) for mitochondrial studies on intact and permeabilized cells.
- Application of SOPs in experimental studies during Short Term Scientific Missions and feeding of data into the MITOEAGLE data repository.
- Publication of SOPs and study results.
Milestones
- Consensus on protocols, reporting schemes and work assignments.
- Completed SOPs for cell preparation & laboratory protocols.
- Application study finished and data transmitted to MITOEAGLE data repository.
- Publication finished.
Deliverables
- SOPs for blood cell separation and respirometric characterisation open for the research community.
- MITOEAGLE data repository for comparative data evaluation, planning of future studies, data mining.
- Publication with a set of reference data.
Feedback WG4
- Dr. Ali will work within the realms of WG4 (blood and cultured cells) and potentially WG1 (protocol development) as well as WG5 (dissemination and training). He will participate in data generation, quality control, and repository efforts of protocols recommended by these working groups. Dr. Ali will contribute to training workshops and conferences that aim at defining standard procedures and terminology in the field of mitochondria physiology. - Sameh S Ali (2016).
- I am very interested in being part of the management committee either as a MC member for managment tasks or in working group 4 MITOEAGLE data repository for blood cells and cultured cells. I have experience in analyzing mitochondrial function in different human tissues as cultured cells and blood cells (also subsets of specific blood populations, see list of publications), muscle and isolated mitochondria. This together with my experience in organizing summer schools and conferences could be helpful as MC member taking care of management tasks. However, if you think my expertise is more helpful in working group 4, I am looking forward to support this working group. - Kathrin Renner-Sattler (2016).