Difference between revisions of "MitoEAGLE protocols, terminology, documentation"
From Bioblast
| Line 34: | Line 34: | ||
:::: It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease. | :::: It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease. | ||
== WG1 Participants == | |||
{{#ask:[[MitoPedia topic::EAGLE]] [[In country::+]] [[Has COST WG1::+]] | |||
| mainlabel=- | |||
|?In country=Country | |||
|?In city=City | |||
|?=Contact | |||
|sort=In country | |||
|order=ascending | |||
|limit=5000 | |||
|format=broadtable | |||
|offset=0 | |||
}} | |||
== WG1 Management == | == WG1 Management == | ||
| Line 120: | Line 132: | ||
::: '''Best practices''' for implementation of analytical and process standards. | ::: '''Best practices''' for implementation of analytical and process standards. | ||
Revision as of 14:50, 27 April 2016
 |
News and Events |  |
 |
Working Groups | Short-Term Scientific Missions | Management Committee | Members |  |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
MitoEAGLE protocols, terminology, documentation
WG1
- Standard operating procedures and user requirement document: Protocols, terminology, documentation
WG1 Description
- The quality management system, scope of SOPs to be elaborated and the outline of the data repositories will be defined in a user requirement document.
Respirometric reference protocols
- A ‘library of protocols’ applied in mitochondrial respiratory physiology will be collected in a standard format, delineating the diversity of experimental approaches to assess bioenergetic function. A set of reference protocols will rigorously document (Maelstrom Research program) the state-of-the-art standards in designing, conducting, reporting, interpreting, and validating such bioenergetic tests (see also Fig. 3).
Interlaboratory proficiency test
- Inter-laboratory ring tests are a basic requirement for quality management. Such proficiency tests require a reference sample which must be homogenous, stable, representative of the diagnostic target, amenable for distribution, and economical for large-scale production. For respiratory OXPHOS analysis a reference sample of structurally and functionally intact mitochondria is not yet available to perform corresponding ring tests (in contrast to enzymatic OXPHOS ring tests). Recently, the widely applied human cell line HEK 293T was found to be potentially suitable for cryopreservation. Upon thawing the cells can be used immediately for respirometry of both intact and permeabilized cells. Thus interlaboratory proficiency testing may now be feasible as a world-wide innovation in the field of mitochondrial respiratory physiology. Participating labs may implement the test for intra- and interlaboratory validation and longitudinal performance monitoring, using a reference OXPHOS protocol (Fig. 3). The organization of the proficiency test should follow the requirements of the ISO 17043 and 13528 standards. A corresponding MITOEAGLE proficiency training shall be developed and implemented. Feedback from the participants will lead to a final adjustment of the SOPs in the proficiency test, which will be made generally available.
Instrumental platforms
- Comparison of results between and within instrumental platforms: The reference protocols and proficiency test will not define the instrumental platform, but will allow a quantitative comparison of results obtained with specific equipment available in the participating laboratories.
Nomenclature
- Harmonization of nomenclature on mitochondrial respiratory states and control parameters: The logistics of development of a database requires application of strictly defined terms for all included variables. There is no general reference available upon which a consistent terminology on mitochondrial physiology and bioenergetics can be based. The MITOEAGLE consortium, therefore, has to accomplish the ambitious goal to unify and simplify the terminology in the field for the purpose of the QMS, which will lead to the development of recommendations for the use of a common terminology in mitochondrial physiological research. A publication will be prepared as an Open Access article which will be a milestone towards a unification of concepts and nomenclature.
Knowledge management system
- With an increase in data volume and complexity comes along the requirement for standardized report formats and digital accessibility of the data. Since such a database is still missing for mitochondrial physiology, the goal of WG1 is to establish an integrated and analytic environment linking mitochondrial respiration with external data (cell physiology, anthropometry, spiroergometry, lifestyle, genomics) in the context of physical fitness evaluation and clinical diagnostics. Databases will be established combining biological and clinical data comprising proprietary and publicly available datasets into one integrated solution. Data import and export functionalities will allow data upload and mapping to corresponding objects in the database, in this way generating valuable data and allowing for project-specific as well as cross-project data mining.
- The ability to formulate meta-studies in our approach is an important success factor: The platform can aggregate the project’s complete knowledge and provides the ability to make diagnostic profiling associated with diseases. In addition to functional data, other data types such as nutritional or environmental factors associated with disease prevention can be identified and aggregated into the MITOEAGLE Knowledge Management System.
- Querying the heterogeneous data types and result representation are the most important requirements of the knowledge platform besides to data integration and modelling. The application will feature different types of visualization techniques for all integrated data types. The database will be developed using the concept of multi-tier client-server architecture. Individual components are separated corresponding to their functionality. An upstream web server handles user requests, an application server performing business logic, and a back-end database system guaranties persistent data storage. All components are additionally separated by means of network security incorporating firewalls and are hosted in a secure environment. The platform administration ensures privacy regarding proprietary data by user rights policies.
- It will be the task of WG1 to continuously collect the input from all other WGs, compare, discuss and align SOPs as far as possible, and to finally develop recommendations for quality control, data reporting and data sharing beyond the published record. In addition, WG1 will develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation. Finally, it will provide a summary on strategic dissemination and an education programme for MITOEAGLE. The education programme will ensure that not only presently participating groups will adhere to the set standards but that also groups joining in at later points may smoothly fit into the community and help to further broaden and deepen our understanding on mitochondrial physiology in health and disease.
WG1 Participants
| Country | City | Contact |
|---|---|---|
| Armenia | Yerevan | Trchounian K |
| Australia | Nedlands | Perks Kara L |
| Australia | Melobourne | Botella Javier |
| Australia | Southport | Vidimce Josif |
| Australia | Melbourne | Whitfield Jamie |
| Australia | Burwood | Trewin Adam J |
| Australia | Melbourne, VIC | Muccini Anna Maria |
| Australia | Parkville | Hardee Justin P |
| Australia | Melbourne | Saner Nicholas |
| Australia | Geelong, Victoria | McKenzie Matthew |
| Australia | Melbourne | Stepto Nigel K |
| Australia | Newtown, Sydney | Stocker Roland |
| Australia | Syndey | Ton Riccardo |
| Australia | Melbourne | Hewakapuge Sudinna |
| Australia | Victoria | Winwood-Smith Hugh |
| Australia | Queensland | Dong Lan-Feng |
| Australia | Victoria | Van Bergen Nicole J |
| Australia | Melbourne | Ferri Alessandra |
| Australia | Melbourne | Lionett S |
| Australia | Clayton Victoria | Alton Lesley |
| Australia | Melbourne | Cassar Samantha |
| Australia | Melbourne | Coughlan Melinda T |
| Australia | Sydney | Hoehn Kyle L |
| Australia | Queensland | Radenkovic Filip |
| Australia | Melbourne | Kuang Jujiao |
| Australia | Melbourne | Genders Amanda J |
| Australia | Sydney | Krycer James R |
| Australia | Clayton | Wolff Jonci Nikolai |
| Australia | Queensland | Fisher Joshua J |
| Australia | Queensland | Holland Olivia J |
| Australia | Nedlands | Filipovska Aleksandra |
| Austria | Innsbruck | Meszaros Andras T |
| Austria | Innsbruck | Thapa Maheshwor |
| Austria | Innsbruck | Doerrier Carolina |
| Austria | Innsbruck | Plangger Mario |
| Austria | Innsbruck | Schartner Melanie |
| Austria | Innsbruck | Goebel G |
| Austria | Innsbruck | Sobotka Ondrej |
| Austria | Innsbruck | Di Marcello Marco |
| Austria | Innsbruck | Bergmeister Lisa |
| Austria | Kapferer Werner | |
| Austria | Innsbruck | Garcia-Souza Luiz F |
| Austria | Innsbruck | Antunes Diana |
| Austria | Innsbruck | Klocker Helmut |
| Austria | Innsbruck | Cardoso Luiza HD |
| Austria | Innsbruck | Perez Valencia Juan Alberto |
| Austria | Innsbruck | Laner Verena |
| Austria | Innsbruck | Gallee Leon |
| Austria | Innsbruck | Karabatsiakis Alexander |
| Austria | Innsbruck | Gnaiger Erich |
| Austria | Innsbruck | Schmitt Sabine |
| Austria | Innsbruck | Schwarzer Christoph |
| Austria | Aldrans | Haider Markus |
| Austria | Klosterneuburg | Sazanov Leonid A |
| Austria | Innsbruck | Cecatto Cristiane |
| Austria | Innsbruck | Nindl Veronika |
| Austria | Innsbruck | Passrugger Manuela |
| Austria | Salzburg | Kofler B |
| Austria | Vienna | Kozlov Andrey V |
| Austria | Innsbruck | Burtscher Martin |
| Austria | Innsbruck | Keller Markus A |
| Austria | Innsbruck | Hoppel Florian |
| Belgium | Leuven, Flanders | Baekelandt V |
| Belgium | Namur | Arnould Thierry |
| Belgium | Brussels | Sonveaux P |
| Belgium | Liege | Votion Dominique-Marie |
| Belguim | Sart Tilman (Liège) | Kruse C |
| Brasil | Porto Alegre | Haas Clarissa |
| Brasilia | Limeira | Ropelle Eduardo R |
| Brazil | Sao Paulo | Vercesi Anibal E |
| Brazil | Rio de Janeiro | Galina Antonio |
| Brazil | Rio de Janeiro | Vieyra Adalberto |
| Brazil | Santa Maria - RS | Goncalves Debora Farina |
| Brazil | Santa Maria - Rio Grande do Sul | Soares Felix Alexandre Antunes |
| Brazil | São Paulo | Kowaltowski Alicia J |
| Brazil | Jaboticabal | Oliveira Marcos Tulio |
| Brazil | Sao Paulo | Silber Ariel Mariano |
| Brazil | Rio de Janeiro | Oliveira Marcus F |
| Brazil | São Paulo | Crispim Marcell |
| Canada | Moncton, NB | Pichaud Nicolas |
| Canada | Edmonton (Alberta) | Lemieux Helene |
| Canada | Edmonton | Zaugg Michael |
| Canada | Saint John | Pulinilkunnil Thomas |
| Canada | Edmonton | Han Woo Hyun |
| Canada | Vancouver, BC | Tausan Daniel |
| Canada | Winnipeg | Chowdhury Subir Roy |
| Canada | Quebec | Joseph Vincent |
| Canada | Rimouski | Munro Daniel |
| Canada | Charlottetown | Adiele Reginald C |
| Canada | Edmonton | Lucchinetti Eliana |
| Canada | Rimouski | Blier Pierre U |
| Canada | Hamilton | Tarnopolsky Mark A |
| Canada | Guelph | Holloway Graham P |
| Canada | Antigonish | Weir M |
| Canada | Ottawa | Darveau Charles-A |
| Canada | Vancouver | Bovard Josh |
| Canada | Montréal | Breton Sophie |
| Canada | Hamilton | Scott Graham R |
| Canada | Ottawa | Harper Mary-Ellen |
| Canada | Montreal, Quebec | Bergdahl Andreas |
| Chile | Coyhaique | Jana Prado Fabian |
| China | Xi`an | Liu Jiankang |
| China | Tianjin | Zhang Yong |
| China | Pukou, Nanjing, | Gan Zhenji |
| China | Fuzhou, Fujian Province | Chen Qi |
| Croatia | Split | Ljubkovic M |
| Croatia | Zagreb | Saric A |
| Croatia | Zagreb | Curik I |
| Croatia | Zagreb | Rubelj Ivica |
| Czech Republic | Ceske Budejovice | Zikova Alena |
| Czech Republic | Pardubice | Handl Jiri |
| Czech Republic | Praha 2 | Danhelovska Tereza |
| Czech Republic | Vestec | Ezrova Zuzana |
| Czech Republic | Vestec | Rohlena Jakub |
| Czech Republic | Praha 2 | Zdrazilova Lucie |
| Czech Republic | Hradec Kralove | Stankova Pavla |
| Czech Republic | Hradec Kralove | Cervinkova Zuzana |
| Czech Republic | Vestec | Davidova Eliska |
| Czech Republic | Pardubice | Rousar Tomas |
| Czech Republic | Prague | Drahota Zdenek |
| Czech Republic | Prague 10 | Urban Tomas |
| Czech Republic | Prague | Krizova J |
| Czech Republic | Prague | Houstek Josef |
| Czech Republic | Hradec Kralove | Endlicher Rene |
| Czech Republic | Plzen -Severni predmesti | Jedlicka Jan |
| Czech Republic | Hradec Kralove | Kucera Otto |
| Czech Republic | Pardubice | Majtnerova Pavlina |
| Czech rep | Hradec Kralove | Falaye T |
| Denmark | Copenhagen | Dela Flemming |
| Denmark | Copenhagen N | Mortensen OH |
| Denmark | Aarhus N | Boetker Hans Erik |
| Denmark | Copenhagen N | Scheibye-Knudsen Morten |
| Denmark | Copenhagen | Nielsen B |
| Denmark | Hvidovre | Nehlin Jan O |
| Denmark | Copenhagen | Larsen Steen |
| Denmark | Copenhagen | Soendergaard SD |
| Denmark | Copenhagen N | Maise Chroeis K |
| Denmark | Copenhagen | Kraunsoee R |
| Denmark | Copenhagen | Gonzalez-Franquesa Alba |
| Denmark | Roskilde | Dalgaard Louise T |
| Denmark | Aarhus N | Jespersen Nichlas Riise |
| Egypt | Cairo | Abdel-Rahman Engy Ali |
| Egypt | Cairo | Ali Sameh S |
| Estonia | Tallinn | Puurand Marju |
| Estonia | Tallinn | Klepinin Aleksandr |
| Estonia | Tallinn | Laasmaa Martin |
| Estonia | Tallinn | Kaambre Tuuli |
| Estonia | Tallinn | Karro N |
| Estonia | Tallinn | Shevchuk Igor |
| Estonia | Tallinn | Truu Laura |
| Estonia | Tallinn | Tepp Kersti |
| Estonia | Tallinn | Vendelin Marko |
| Estonia | Tallinn | Klepinina Lyudmila |
| Estonia | Tartu | Paju Kalju |
| Finland | Helsinki | Suomalainen Wartiovaara Anu |
| Finland | Turku | Stier Antoine |
| Finland | Helsinki | Jackson Christopher Benjamin |
| Finland | Jyvaeskylae | Kainulainen Heikki |
| France | Martinique | Neviere Remi |
| France | Angers | Procaccio Vincent |
| France | Paris | Armand Anne-Sophie |
| France | Paris | Bouillaud Frederic |
| France | Maisons-Alfort Cedex | Prola Alexandre |
| France | Tours | Dumas Jean-Francois |
| France | Plouzané | Salin Karine |
| France | Nouzilly | Collin-Chenot Anne |
| France | Angers | Spinazzi Marco |
| France | Bordeaux cedex | Mourier A |
| France | Montpellier | Wrutniak-Cabello Chantal |
| France | Lille | Montaigne David |
| France | Martinique | Cano Sanchez Maria Consolacion |
| France | Bordeaux | Amoedo Nivea Dias |
| France | Bordeaux cedex | Rossignol Rodrigue |
| France | Gueguen Naig | |
| France | Bordeaux | Sarlak Saharnaz |
| Germany | Ulm | Schaefer PM |
| Germany | Bremerhaven | Mark Felix Christopher |
| Germany | Cologne | Maciej Sarah |
| Germany | Hannover | Das Anibh Martin |
| Germany | Mainz | Methner Axel |
| Germany | Giessen | Sommer Natascha |
| Germany | Jena | Szibor Marten |
| Germany | Duesseldorf | Jelenik T |
| Germany | Gießen | Grewal Rekha |
| Germany | Munich | Zischka Hans |
| Germany | Frankfurt | Hamann Andrea |
| Germany | Cologne | Pesta Dominik |
| Germany | Magdeburg | Schoenfeld Peter |
| Germany | Marburg | Vogt Sebastian |
| Germany | Cologne | Wiesner Rudolf J |
| Germany | Wickert Anika | |
| Germany | Wilhelmshaven | Salmon Pablo |
| Germany | Gießen | Silaidos Carmina |
| Germany | Duesseldorf | Piel Sarah |
| Germany | Cologne | Trifunovic Aleksandra |
| Germany | Regensburg | Renner-Sattler Kathrin |
| Germany | Marburg | Ramzan Rabia |
| Germany | Freiburg | Schuele R |
| Germany | Duesseldorf | Haendeler Judith |
| Germany | Doermann Niklas | |
| Germany | Frankfurt am Main | Viel Christian |
| Germany | Rostock | Sokolova Inna |
| Germany | Mainz | Maull Felicia |
| Germany | Frankfurt | Osiewacz Heinz D |
| Germany | Neuherberg | Einer C |
| Germany | Düsseldorf | Goy C |
| Germany | Biberach an der Riß | Nold V |
| Germany | Frankfurt | Warnsmann Verena |
| Germany | Duesseldorf | Roden Michael |
| Germany | Tuebingen | Kappler Lisa |
| Germany | Cologne | Ho Dieu Hien |
| Germany | Giessen | Schulz Rainer |
| Greece | 15771 | Andreadou Ioanna |
| Greece | Thessaloniki | Lazou Antigone |
| Greece | Athens | Trougakos Ioannis P |
| Greece | Heraklion | Tavernarakis Nektarios |
| Greece | Athens | Gumeni Sentiljana |
| Hungary | Szeged | Ferdinandy Peter |
| Hungary | Budapest | Chinopoulos Christos |
| Hungary | Budapest | Tretter Laszlo |
| Hungary | Budapest | Komlodi Timea |
| Hungary | Szeged | Boros Mihaly |
| Hungary | Debrecen | Verebne Tar K |
| Hungary | Budapest | Horvath G |
| India | Hyderabad | Jha Rajan Kumar |
| India | Lucknow | Gayen Jiaur |
| India | Kollam | Suravajhala Prashanth |
| India | Haryana | Chakrabarti Sasanka |
| India | Varanasi | Sonkar Vijay K |
| India | New Delhi | Thakkar Himani |
| India | New Delhi | Vincent Vinnyfred |
| Iran | Tehran | Shirazi Reza |
| Iran | Tehran | Safaei Zahra |
| Ireland | Dublin | Porter Richard K |
| Ireland | Dublin | O'Gorman Donal |
| Israel | Zrifin | Sova Marina |
| Israel | Beer Sheva | Mishmar Dan |
| Israel | Jerusalem | Saada Reisch Ann |
| Israel | Rishon Le Zion | Hachmo Yafit |
| Italia | Udine | Salvadego Desy |
| Italien | Padova PD | Szabo Ildiko |
| Italy | Rome | Scatena Roberto |
| Italy | Milano | Bottoni P |
| Italy | Ancona | Battino Maurizio |
| Italy | Bologna | Paterlini S |
| Italy | St. Lorenzen | Harrison David K |
| Italy | Bologna | Porcelli AM |
| Italy | Padova | Viscomi Carlo |
| Italy | Udine | Grassi B |
| Italy | Catania | Messina Angela |
| Italy | Bolzano | Zanon Alessandra |
| Italy | Padova | Bernardi Paolo |
| Italy | Bologna | Gasparre G |
| Italy | Milan | Clementi Emilio |
| Italy | Catania | De Pinto Vito |
| Italy | Bolzano/Bozen | Volani Chiara |
| Italy | Padova | Fernandez-Vizarra Erika |
| Italy | Bari | Attimonelli M |
| Italy | Monserrato | Isola Raffaella |
| Italy | Catania | Magri Andrea |
| Italy | Campobasso | Pallotta Maria Luigia |
| Italy | Bologna | Genova Maria Luisa |
| Japan | Itabashi-ku Tokyo | Tanaka Masashi |
| Japan | Sapporo | Matsumoto J |
| Kitzbühel | Innsbruck | Fischer Michael J |
| Korea | Seoul | Pak Youngmi Kim |
| Korea | Incheon | Kwak Hyo Bum |
| Korea (South) | Seoul | Lee Hong Kyu |
| Latvia | Riga | Svalbe Baiba |
| Latvia | Riga | Zvejniece Liga |
| Latvia | Riga | Stelfa Gundega |
| Latvia | Riga | Liepins Edgars |
| Latvia | Riga | Volska Kristine |
| Latvia | Riga | Inashkina I |
| Latvia | Riga | Jansone Baiba |
| Latvia | Riga | Dambrova Maija |
| Latvia | Riga | Vilks Karlis |
| Luxembourg | Strassen | Devaux Yvan |
| Malaysia | Kuala Lumpur | Hassan Hazirah |
| Malta | Msida | Vella Joanna |
| Mexico | Tlalpan | Rodriguez-Enriquez Sara |
| Mexico | Mexico City | Aparicio Trejo OE |
| México | San Pedro Garza García, Nuevo Leon | Garcia-Rivas Gerardo |
| Netherlands | Maastricht | Grevendonk L |
| Netherlands | GA Wageningen | Grefte Sander |
| New Zealand | Auckland | Hickey Anthony J |
| New Zealand | Auckland | Kaur Sarbjot |
| New Zealand | Auckland | Ward Marie Louise |
| New Zealand | Wellington | Berridge Michael V |
| Norway | Bergen | Roesland Gro Vatne |
| Norway | Bergen | Pettersen Nitschke Ina Katrine |
| Norway | Bergen | Hoel Fredrik |
| Norway | Bergen | Tronstad Karl Johan |
| Norway | Tromsoe | Boardman Neoma T |
| Norway | Trondheim | Grill V |
| Norway | Bergen | Blindheim Dan Filip |
| Norway | Bergen | Berge Rolf K |
| Norway | Oslo | Eide L |
| Norway | Bergen | Dyrstad Sissel E |
| Norway | Bergen | Haavik Jan |
| Norway | Trondheim | Smenes Benedikte Therese |
| Norway | Trondheim | Lerfall Joergen |
| Norway | Trondheim | Rognmo O |
| Poland | Gdańsk | Kaczor Jan Jacek |
| Poland | Lodz | Labieniec-Watala Magdalena |
| Poland | Warsaw | Sek Aleksandra |
| Poland | Warsaw | Karkucinska-Wieckowska Agnieszka |
| Poland | Warsaw | Szewczyk Adam |
| Poland | Warsaw | Wasilewski M |
| Poland | Warsaw | Bednarczyk Piotr |
| Poland | Warsaw | Wieckowski Mariusz R |
| Poland | Warsaw | Kampa Rafal Pawel |
| Poland | Warszawa | Jaskiewicz Anna |
| Poland | Warsaw | Zablocki Krzysztof |
| Poland | Krakow | Dembinska-Kiec Aldona |
| Poland | Lodz | Watala Cezary |
| Poland | Warsaw | Dymkowska Dorota |
| Poland | Poznan | Jarmuszkiewicz Wieslawa |
| Portugal | Cantanhede | Amorim Ricardo |
| Portugal | Aveiro | Vitorino Rui Miguel Pinheiro |
| Portugal | Coimbra | Dias Candida |
| Portugal | Porto | Beleza Jorge |
| Portugal | Porto | Alves Marco G |
| Portugal | Coimbra | Duarte Filipe Valente |
| Portugal | Coimbra | Rolo Anabela Pinto |
| Portugal | Coimbra | Oliveira Paulo J |
| Portugal | Coimbra | Grilo Luis |
| Portugal | Coimbra | Minuzzi Luciele M |
| Portugal | Lisboa | Macedo Maria Paula |
| Portugal | Aveiro | Ferreira Rita Maria P |
| Portugal | Porto | Oliveira Jorge |
| Portugal | Porto | Oliveira Pedro Fontes |
| Portugal | Cantanhede | Teodoro Joao Soeiro |
| Portugal | Coimbra | Palmeira Carlos |
| Portugal | Cantanhede | Rodrigues Ana Sofia |
| Portugal | Coimbra | Santos Diana |
| Portugal | Porto | Crisostomo Luis |
| Portugal | Porto | Moreira Bruno P |
| Portugal | Porto | Silva Ana Maria |
| Qatar | Doha | Guarch Meritxell Espino |
| Republic of Ghana | Cape Coast | Tei BN |
| Republic of Korea | Busan | Han Jin |
| Republic of Korea | Yuseong-gu Daejeon | Quang Don T |
| Republic of Korea | Daejeon | Park SH |
| Republic of Korea | Busan | Kim Hyoung Kyu |
| Republic of Serbia | Belgrade | Lalic Nebojsa M |
| Republic of Serbia | Novi Sad | Andric Silvana |
| Republic of Serbia | Belgrade | Krako Jakovljevic Nina |
| Russia | Moscow | Vinogradov Andrey D |
| Russia | Moscow | Pavlova Nadia |
| Russia | Yoshkar-Ola | Dubinin M |
| Serbia | Belgrade | Misirkic Marjanovic Maja |
| Serbia | Belgrade | Mandic M |
| Serbia | Belgrade | Djordjevic M |
| Serbia | Belgrade | Savkovic U |
| Singapore | Singapore | Singh Brijesh Kumar |
| Singapore | Singapore | Hausenloy Derek J |
| Slovakia | Košice | Cizmarova Beata |
| Slovakia | Bratislava | Sumbalova Zuzana |
| Slovakia | Bratislava 45 | Ferko Miroslav |
| Spain | A Coruña | Blanco FJ |
| Spain | Granada | Hidalgo-Gutierrez A |
| Spain | Madrid | Moran M |
| Spain | Valencia | Casado Pinna Marta |
| Spain | Barcelona | Gil J |
| Spain | Barcelona | Gama Perez Pau |
| Spain | Granada | Quiles JL |
| Spain | Barcelona | Garcia-Roves Pablo Miguel |
| Spain | Sevilla | Bustos Matilde |
| Spain | Madrid | Hernansanz-Agustin Pablo |
| Spain | A Coruña | Mayan MD |
| Spain | Barcelona | De la Torre Lara J |
| Spain | Barcelona | Zorzano Antonio |
| Spain | Salamanca | Almeida Angeles |
| Spain | Las Palmas de Gran Canaria | Calbet Jose AL |
| Spain | Madrid | Rial Eduardo |
| Spain | Madrid | Aragones Lopez J |
| Spain | Armilla (Granada) | Fernandez-Ortiz Marisol |
| Spain | Barcelona | Perales Jose Carles |
| Spain | Barcelona | Goncalo Teixeira da Silva Rui |
| Spain | Barcelona | Bravo-Sagua Roberto |
| Sweden | Stockholm | Nedergaard J |
| Sweden | Stockholm | Larsen Filip J |
| Sweden | Stockholm | Shabalina Irina G |
| Sweden | Gothenburg | Bhuvanachandran NS |
| Sweden | Lund | Ehinger Johannes K |
| Sweden | Stockholm | Cardinale Daniele A |
| Switzerland | Bern | Wyss RK |
| Switzerland | Basel | Bouitbir Jamal |
| Switzerland | Lausanne | Lagarrigue Sylviane |
| Switzerland | Basel | Fornaro Mara |
| Switzerland | Zurich | Gorr Thomas A |
| Switzerland | Lausanne | Place Nicolas |
| Switzerland | Bern | Nuoffer Jean-Marc |
| Switzerland | Lausanne | Morato Fornaguera L |
| Switzerland | Lausanne | Donnelly Chris |
| Switzerland | Zürich | Zaugg Kathrin |
| Switzerland | Lausanne | Weger M |
| Switzerland | Lausanne | Zanou Nadege |
| Switzerland | Lausanne | Zalachoras I |
| Switzerland | Lausanne | Amati Francesca |
| Switzerland | Lausanne | Burtscher Johannes |
| Switzerland | Lausanne | Canto Alvarez Carles |
| Switzerland | Lausanne | Sandi Carmen |
| Switzerland | Lausanne | Gebara E |
| Taiwan | Changhua City | Wei Yau-Huei |
| The Netherlands | Maastricht | Nabben Miranda |
| The Netherlands | BT Amsterdam | Wuest Rob CI |
| The Netherlands | Groningen | Bakker Barbara M |
| The Netherlands | Delft | McMillan Duncan GG |
| The Netherlands | Maastricht | Schrauwen Patrick |
| UK | Cambridge | Brown Guy C |
| UK | Canterbury, Kent | Gourlay Campbell W |
| UK | London | Lane Nick |
| UK | London | Bettinazzi Stefano |
| UK | London | Rodriguez Enrique |
| USA | Little Rock | Parajuli Nirmala |
| USA | Warminster | Orynbayeva Zulfiya |
| USA | Chicago | Dayanidhi Sudarshan |
| USA | Kansas City | Thyfault John P |
| USA | Needham | Brown David A |
| USA | Winston-Salem | Gonzalez-Armenta Jenny L |
| USA | New York | Galkin Alexander |
| USA | Indianapolis | Brozinick Joseph T |
| USA | Corvallis | Batterson Philip M |
| USA | Cambridge | Wohlwend Martin |
| USA | Boston | Kristal Bruce S |
| USA | Iowa City | Chaurasia Bhagirath |
| USA | Fairbanks | O'Brien Kristin |
| USA | Atlanta | Jang Young Charles |
| USA | Miami | Fontanesi F |
| USA | St. Louis | Abumrad Nada A |
| USA | Baltimore | Gonzalez-Freire Marta |
| USA | Winston-Salem | Ahn Bumsoo |
| USA | San Diego | Patel Hemal H |
| USA | Birmingham | Moellering Douglas R |
| USA | Cleveland | Hoppel Charles L |
| USA | Iowa City | Wagner Brett A |
| USA | Fort Collins | Chicco Adam J |
| USA | Stillwater | Davis Michael S |
| USA | Torrance | Rossiter Harry B |
| USA | Rochester | Nair K Sreekumaran |
| USA | Akron | Cohen Bruce H |
| USA | Jupiter | Trivigno Catherine |
| USA | East Lansing | Zhang Yizhu |
| USA | Davis | Hellgren Kim T |
| USA | Philadelphia | Ganetzky Rebecca |
| USA | Pittsburgh | Jurczak Michael J |
| USA | Orlando | Sparks Lauren M |
| USA | San Diego; La Jolla | Molina Anthony JA |
| USA | Gainesville | Leeuwenburgh Christiaan |
| USA | Provo | Hancock Chad R |
| USA | St. Louis | Pietka Terri A |
| USA | Philadelphia | Jang David H |
| USA | Los Angeles | Kwast Kurt E |
| USA | Davis | Adams Sean H |
| USA | San Diego | Cannon Daniel T |
| USA | Norfolk | Lai Nicola |
| USA | Columbus | Sims Carrie A |
| USA | Pittsburgh | Prochownik Edward V |
| USA | Seattle | Marcinek DJ |
| USA | Galveston | Durham William J |
| USA | Aurora | Ford Ellen |
| USA | Ann Arbor | Tyrrell Daniel J |
| USA | Philadelphia | Towheed Atif |
| USA | Corvallis | Newsom Sean A |
| USA | Rochester | Lanza Ian R |
| USA | Omaha | Kim Julian KS |
| USA | Milwaukee | Dash Ranjan K |
| USA | Kansas City | Swerdlow Russell H |
| USA | Puskarich Michael A | |
| USA | Bethesda | Sharma Pushpa |
| USA | Colorado Springs | Jacobs RA |
| USA | Denton | Dzialowski Edward M |
| USA | Cleveland | Tandler Bernard |
| USA | Portland | Garlid Keith D |
| USA | Orlando | Coen Paul M |
| USA | Orlando | Goodpaster Bret H |
| USA | Fayetteville | Iyer Shilpa |
| USA | Denver | Van Hove Johan |
| USA | Berkeley | Williams Caroline M |
| USA | Baton Rouge | Irving Brian A |
| USA | Stanford | Stary Creed |
| USA | Durham | Li Pingan Andy |
| USA | La Jolla | Valentine Joseph Marco |
| USA | Aurora | Sparagna Genevieve C |
| USA | El Paso | Bajpeyi Sudip |
| USA | Baton Rouge | Hand Steven C |
| USA | Corvallis | Robinson Matthew M |
| USA | Bethesda | Fessel Joshua Patrick |
| USA | Fayetteville | Rao RR |
| USA | Fairbanks | Coker Robert H |
| USA | Philadelphia | Falk Marni J |
| USA | Little Rock | MacMillan-Crow Lee Ann |
| USA | Louisville | Skolik Robert A |
| USA | Gainesville | Neyroud D |
| USA | Nashville | Wasserman David H |
| USA | Orlando | Pino Maria F |
| USA | Little Rock | Borsheim Elisabet |
| USA | Aurora | Moreau Kerrie |
| ... further results | ||
WG1 Management
Tasks
- Opening of the database to the research public for data search and moderated entry of new data sets.
- Development of interlaboratory proficiency ring test, of recommendation/certification reports and of a MITOEAGLE proficiency training module (with WG5).
- Sample distribution, ring test experiments (MITOEAGLE-proficiency training on two different points of time, 6 months apart), data analysis by experimenters and MITOEAGLE-proficiency test manager.
- Reports and publication: Reports with specific results in comparison with an anonymous data summary, and joint publication. Individual reports may be complemented by recommendations, and are summarized in an informal certification report (move to WG5).
- Instrumental platform comparison with reference sample applying comparable measurements.
- Draft, dissemination, collection and final formulation of a manuscript on concepts and terminology of mitochondrial physiology (with WG5).
- Collect and discuss procedures and experimental protocols for the evaluation of mitochondrial capacities and create a library of protocols: A database comprising reference SUIT protocols, standard experimental media, and detailed instructions for OXPHOS analysis is assembled.
- MITOEAGLE data management system (DMS) development: The DMS for further data preprocessing. The DMS will enable management and analysis of instrument-specific and project-specific data.
- MITOEAGLE database (DB) development: DB will facilitate high-level project specific analysis as well as meta-analyses across projects, visualization of project specific data as well as integrative analyses of datasets for various proprietary and/or publicly available datasets.
- Testing of developed software components regarding functionality and usability, modification and implementation. Deployment for routine testing in the labs of our strategic partners.
- Data input: Upload of datasets generated through WG 2-4 in internal lab developments, results of the proficiency ring test, and during application module developments. In addition, the database will be populated with available proprietary and public datasets.
- Develop recommendations for quality control, data reporting and data sharing beyond the published record. Develop concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation (move to WG5).
- Provide a summary on strategic dissemination and an education programme for MITOEAGLE (move to WG5).
Milestones
- Data sets entered in standardized format and database opened for the public
- Protocols for ring-tests and standard analysis and report scheme defined
- MITOEAGLE proficiency training workshop finished (move to WG5)
- Analysis is completed and joint publication submitted (with WG5)
- Publication of paper on concepts and terminology (with WG5)
- Library of protocols online
- Publication of recommended protocols and procedures
- Guidelines for acquisition, evaluation and data documentation available (with WG5)
- MITOEAGLE data management system finished
- MITOEAGLE database ready for use
- Software modifications incorporated following extensive tests
- Publication of MITOEAGLE-KMP
- Recommendations on data acquisition, evaluation and management published (with WG5)
- Concepts on Open Access and institutionalized service for data management, data mining and health-care conforming standards of data interpretation published (move to WG5)
Deliverables
- Guidelines for future research and recommendations for the evaluation of respiratory characteristics in human respirometric samples (with WG5)
- A database on mt fitness evaluated with human cells and tissues
- Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation (move to WG5)
- Qualitative and quantitative evaluation of results obtained in ongoing studies by comparison with a reference sample
- An expanded MITOEAGLE database
- A joint publication documenting the results of the MITOEAGLE-PT (with WG5)
- A multi-authored publication towards a unification of concepts and nomenclature in mitochondrial physiology (with WG5)
- A library of protocols for sample preparation and examination by respirometry
- A multi-authored publication with recommendations of comparable standard protocols and procedures (with WG5)
- Guidelines as to the optimum use of a reference sample for determination of respiration and related parameters of interest
- The MITOEAGLE data management system
- A continuously updated MITOEAGLE database for the use as reference data and guideline towards application of optimized protocols, ultimately leading towards standardization.
- A joint publication on the use of the MITOEAGLE database and management system (with WG5)
- An education programme for the use of MITOEAGLE database and management system (move to WG5).
Projects in progress
- Interlaboratory proficiency ring test
- Testing of software components
- Concepts and terminology on mitochondrial physiology
- Procedures and experimental protocols for the evaluation of mitochondrial capacities, library of protocols
- Data management system (DMS)
- Database
Next steps
- Best practices for implementation of analytical and process standards.
References
- General requirements for the competence of testing and calibration laboratories » ISO/IEC 17025:2005
