From Bioblast
NextGen-O2k Scientific developments
The revolutionary all-in-one instrument to conquer mitochondrial disease.
WP2: Scientific developments
Abstract
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
- Determination of optimal Q-sensor measurement conditions
- Determination of optimal measurement conditions for the PB-Module
- Development of protocols for scientific, pre-clinical and algal biotechnology applications
- Data handling and quality control
- The aim of this workpackage is to test measurement conditions for the newly developed modules and the development of specific applications.
Achievements
- Project Year 1: During testing in the first project year, optimal measurement conditions were determined for using the Q-sensor with a variety of biological samples. Simultaneously testing began of the beta version of DatLab. New SUIT protocols were developed for general, pre-clinical, and algal biotechnology applications. A focus has also been given to internal and external quality control measures.
- Project Year 2: During the second project year, measurement conditions and protocols were optimized for the PB- and Q-Modules with a variety of biological samples. Additionally, the NADH-Module for evaluation of the NADH-pool in physio-pathological conditions was integrated in the NextGen-O2k. Testing of modules for the DatLab8 software were conducted and improvements applied. Moreover, SUIT protocols for general, pre-clinical, and algal biotechnology applications were developed. Quality control was maintained during the second period of project, an important factor in the development of the NexGen-O2k instrument and relevant protocols.
Progress and next steps
See Β» Track record
Luiza Cardoza, PhD. testing the Q-Module and DatLab8
NextGen-O2k MitoFit Preprints
Link | View | Reference | Year |
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Donnelly 2023 MitoFit | MitoFit Preprints 2023.2. Functional hypoxia reduces mitochondrial calcium uptake. | Donnelly C, KomlΓ³di T, Cecatto C, Cardoso LHD, Compagnion AC, Matera A, Tavernari D, Zanou N, Kayser B, Gnaiger E, Place N (2023) Functional hypoxia reduces mitochondrial calcium uptake. MitoFit Preprints 2023.2. https://doi.org/10.26124/mitofit:2023-0002 β 2024-11-17 published in Redox Biol. | 2023 |
Went 2021 MitoFit PB | MitoFit Preprints 2021.05. | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005 | 2021 |
Huete-Ortega 2020 MitoFit Preprint Arch EA | MitoFit Preprint Arch EA20.1. High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii β towards biotechnology exploitations | Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii β towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.0001 | 2020 |