Difference between revisions of "PGMS-pathway control state"
| Line 26: | Line 26: | ||
:::*[[SUIT-008]] | :::*[[SUIT-008]] | ||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-008 O2 pfi D014]] | ::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-008 O2 pfi D014]] | ||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-008 O2 pce D025]] | ::::* DL-Protocol for permeabilized cells (pce): [[SUIT-008 O2 ce-pce D025]] | ||
:::*[[SUIT-014]] | :::*[[SUIT-014]] | ||
| Line 38: | Line 38: | ||
:::*[[SUIT-008]] | :::*[[SUIT-008]] | ||
::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-008 O2 pfi D014]] | ::::* DL-Protocol for permeabilized fibers (pfi): [[SUIT-008 O2 pfi D014]] | ||
::::* DL-Protocol for permeabilized cells (pce): [[SUIT-008 O2 pce D025]] | ::::* DL-Protocol for permeabilized cells (pce): [[SUIT-008 O2 ce-pce D025]] | ||
:::*[[SUIT-014]] | :::*[[SUIT-014]] | ||
== Discussion == | == Discussion == | ||
:::: Recent studies showed that S- and NS-linked OXPHOS capacity is inhibited by 2 mM malate concentrations as applied in many SUIT protocols. This inhibition is less pronounced at higher succinate concentrations (10 mM up to 50 mM S). | :::: Recent studies showed that S- and NS-linked OXPHOS capacity is inhibited by 2 mM malate concentrations as applied in many SUIT protocols. This inhibition is less pronounced at higher succinate concentrations (10 mM up to 50 mM S). | ||
Revision as of 11:46, 27 March 2019
- high-resolution terminology - matching measurements at high-resolution
PGMS-pathway control state
Description
PGMS: Pyruvate & Glutamate & Malate & Succinate.
MitoPathway control state: NS-pathway control state
2-oxoglutarate is produced through the citric acid cycle from citrate by isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase. If the 2-oxoglutarate carrier does not outcompete these sources of 2-oxoglutarate, then the TCA cycle operates in full circle with external pyruvate&malate&glutamate&succinate
Abbreviation: PGMS
Reference: Gnaiger 2014 MitoPathways - Chapter 5.6
MitoPedia concepts:
SUIT state
PGMSL
PGMS pathway in the LEAK state can be evaluated in the following SUIT protocols:
PGMSP
PGMS pathway in the OXPHOS state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
- DL-Protocol for permeabilized cells (pce): SUIT-008 O2 ce-pce D025
-
PGMSE
PGMS pathway in the ET state can be evaluated in the following SUIT protocols:
-
- DL-Protocol for isolated mitochondria and tissue homogenate (mt): SUIT-001 O2 mt D001
- DL-Protocol for permeabilized fibers (pfi): SUIT-001 O2 pfi D002
- DL-Protocol for permeabilized cells (pce): SUIT-001 O2 pce D003
- DL-Protocol for permeabilized PBMCs and PLTs: SUIT-001 O2 pce D004
- DL-Protocol for permeabilized fibers (pfi): SUIT-008 O2 pfi D014
- DL-Protocol for permeabilized cells (pce): SUIT-008 O2 ce-pce D025
-
Discussion
- Recent studies showed that S- and NS-linked OXPHOS capacity is inhibited by 2 mM malate concentrations as applied in many SUIT protocols. This inhibition is less pronounced at higher succinate concentrations (10 mM up to 50 mM S).
