Difference between revisions of "SUIT-010"
From Bioblast
Line 15: | Line 15: | ||
* [[SUIT-010 O2 pce D008]] | * [[SUIT-010 O2 pce D008]] | ||
{{Template:SUIT-010}} | |||
== Strengths and limitations == | |||
:::* [[Digitonin]] is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol. | |||
== Compare SUIT protocols == | |||
::::* [[SUIT-003 O2 ce D028]] | |||
::::* [[SUIT-003]] | |||
::::* [[SUIT-006]] | |||
== References == | == References == | ||
Line 28: | Line 38: | ||
|order=descending | |order=descending | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=MiP concept, SUIT protocol, Recommended | |mitopedia concept=MiP concept, SUIT protocol, Recommended |
Revision as of 10:55, 14 February 2019
Description
Abbreviation: ceS,S
Reference: A Optimization of digitonin concentration for pce »Versions
- SUIT-category: ceS,S
- SUIT protocol pattern: ce1;ce2Rot;ce3S;ce4D;1Dig;1c
SUIT-010 is designed for evaluation of optimum digitonin concentration for permeabilizing cells, a requirement to account for differendes between cell types, concentration of cells, and variability between batches of the natural product digitonin.
Communicated by Cardoso LH, Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-01-29)
Specific SUIT protocols
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2Rot | ROX | ce1;ce2Rot
| ||
ce3S | SL(n) | S | CII | ce1;ce2Rot;ce3S
|
ce4D | SP | S | CII | ce1;ce2Rot;ce3S;ce4D
|
1Dig | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig
|
1c | SP | S | CII | ce1;ce2Rot;ce3S;ce4D;1Dig;1c
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Digitonin is a natural product and thus the effective concentration has to be determined by titrations for every batch. The optimum effective digitonin concentrations for complete plasma membrane permeabilization can be determined with this protocol.
Compare SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Doerrier 2018 Methods Mol Biol | 2018 | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3 | Human Mouse Rat Saccharomyces cerevisiae | Heart Skeletal muscle Endothelial;epithelial;mesothelial cell Blood cells HEK Platelet |
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry