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..., which also allow for harmonization of data obtained with these different protocols.

::::# [[MitoFit protocols]] ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015)

...two protocols are conducted in parallel. Therefore, these harmonized SUIT protocols are complementary with their focus on specific respiratory coupling and pat :::: The cross-linked respiratory states for harmonization between protocols are:

...eft|90px|Carolina Doerrier]] Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers.

...tochondrial function assay: monitoring preparative steps and comparison of protocols. ...in aim of [[WG4]] is also protocols in the harmonization to SUIT reference protocols

...ttachment). We therefore find the focus on harmonization and validation of protocols and terminology especially important.

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-002]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-001]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-002]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-002]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-001]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

== Specific SUIT protocols == :::+ Harmonization with many SUIT protocols (up to step 7S).

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows to: :::+ Harmonization with [[SUIT-002]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-001]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

...ending on the WG4 participants input, cell lines will be defined for which harmonization of datasets should be achieved (Fig. 2). ...k-off meeting to develop a working plan for the establishment of consensus protocols, reporting schemes, and the assignment of specific study tasks.

...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows: :::+ Harmonization with [[SUIT-001]] allows to perform both SUIT protocols in parallel. The cross-linked respiratory states can be statistically used

::: '''[[Categories of SUIT protocols|SUIT-category]]:''' FNS(Oct,PGM) :::+ Harmonization with many SUIT protocols (up to step 7S).

::::::* '''WG1: Harmonization of nomenclature on mitochondrial respiratory states and control parameters: ::::::* '''WG1: Respirometric reference protocols:'''

...odríguez E, Bettinazzi S, Inwongwan S, Camus MF, Lane N (2023) Harmonizing protocols to measure ''Drosophila'' respiratory function in mitochondrial preparation ...concentration and probe. We recommend working towards the harmonization of protocols for assessing mitochondrial function in fruit flies.

== Specific SUIT protocols == ...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows:

:* Harmonization of respirometric protocols comparing isolated mitochondria and permeabilized muscle fibers ...the Action. The STSM will aid in the standardization and harmonization of protocols that increase the value and reduce the noise in mitochondrial research. The

== Specific SUIT protocols == ...y control in a large variety of species, tissues and cell types. Both SUIT protocols provide a mitochondrial mapping which allows:

...on mitochondrial respiration showed a very wide scatter. Harmonization of protocols by direct determination of the effect of various procedures on the results

...anizations: P³G (Public Population Project in Genomics), PHOEBE (Promoting Harmonization of Epidemiological Biobanks in Europe) and CPT (Canadian Partnership for To ...monization. The DataSHaPER supports both 'prospective' and 'retrospective' harmonization.

...ar as possible (using tools such as PubMed Commons). As a result, specific protocols will be summarized for handling muscle tissue. Depending on specific intere :::# Discussion of procedures, protocols and general guidelines.

...s, living cells). Optimized substrate-uncoupler-inhibitor titration (SUIT) protocols offer a powerful tool for comprehensive mitochondrial physiology analysis b #proficiency tests with reference samples for inter-laboratory harmonization[4,5]; and

...e:DoerrierC.JPG|left|90px]] Substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. ...physiology. In conclusion, an optimal design and comprehension of the SUIT protocols is crucial for a proper evaluation of mitochondrial function in healthy and

|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] SUIT reference protocol for OXPHOS analysis by high-resolution respiromet == Harmonized SUIT protocols ==

...E, Garcia-Roves PM (2020) '''Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers.''' - Updated: 2020-03-08 ...E is preparing a manuscript 'Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers'. The group is working on it with Open

...respiratory states, while additional information is obtained when the two protocols are conducted in parallel. Therefore, RP1 and RP2 are complementary with th ...age:MitoFit Training Camp 2016_Doerrier_Figure3.jpg|left|500px]] Figure. 3:Harmonization of RP1&2.

...physiology. In conclusion, an optimal design and comprehension of the SUIT protocols is crucial for a proper evaluation of mitochondrial function in healthy and '''Figure 2.''' Reference protocols: RP1 and RP2. Harmonization between RP1 and RP2. RP1) RP1 which starts with linear coupling control, L

...e electron entries feeding the Q-junction and with cross-linked states for harmonization. Defined coupling and pathway states are established in different mitochond

...dedicated MitoEAGLE knowledge management network developing harmonization protocols towards generating a rigorously monitored data repository on mitochondrial

...Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and is ...pdf|O2k-brief]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]]

...er E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19 ...tofitea19.MiPSchool.0009.pdf Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers]

::::* [[Doerrier 2018 MiP2018a |Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers]]

:::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::» [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]

(2) Harmonization of experimental procedures (protocols) is a basic requirement to increase the robustness of information and knowl ...s to research targeted at hypothesis testing. Experimental optimization of protocols including negative results should be made publicly available at an early ti

...physiology study. An important issue for this goal is the harmonization of protocols for high-resolution respirometry (HRR) with pfi.

...blood collection to cell purification, (''2'') and standardization of SUIT protocols applied in the respirometric analysis. We present the procedures adopted fo ...arcia-Souza LF, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2018) O2k-Protocols: Isolation of blood cells for HRR. Mitochondr Physiol Network 17:1-16.

...019 MitoFit Preprint Arch EA|Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers]]'''''</big>.

...dedicated MitoEAGLE knowledge management network developing harmonization protocols towards generating a rigorously monitored data repository on mitochondrial

...common cross-linked respiratory states, allowing for harmonization of both protocols. The fluxes were corrected for the contribution by contaminating cells.

..., as it offers a large collection of relevant preprints, publications, and protocols, which are always up-to-date and therefore create a platform where recent k ...l in the process of final manuscript preparation, such as harmonization of protocols, which not only help to create clear guidelines for other researchers but a

:::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::» [[MitoEAGLE protocols, terminology, documentation |WG1 Project application]]

...|left|90px|Carolina Anneliese Doerrier]] Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers.

[[File:SUIT-nomenclature.jpg|right|400px|link= MitoPedia: SUIT|SUIT protocols]] === A1: Diagnostic SUIT protocols of mitochondrial fitness ===

...l physiology [2]. A rationale for categorizing PCS helps in selecting SUIT protocols according to the specific research question or diagnostic aim, and is essen ...tic mitochondrial physiology [1]. With emphasis on conceptual differences, harmonization is achieved with the historical terminology in bioenergetics (States 1 to 5

...us lateralis''); (''2'') emphasizes the importance of comparative protocol harmonization projects and reproducibility studies; (''3'') illustrates the necessity and ...a comprehensive review by the MitoEAGLE Working Group 2 (skeletal muscle). Harmonization introduces potential biases with a scope of improvement based on updated ev

...donors: 4 to 5 • Respiration media: MiR05 •Cell type: freshly isolated PLT Protocols (respirometry/Fluorometry): • AmR_SUIT 9_pce: 1S;2D;3P;4Ama • SUIT 7: c

...states and applications of substrate-uncoupler-inhibitor titration (SUIT) protocols. - ''Last update: 2017-07-22'' :::: '''Session C''' (morning) '''OXPHOS protocols and data reporting'''

...ion in health and disease[2]. Therefore, our main goals are to: 1) compare protocols used in different research laboratories, 2) analyze factors which contribut ...er E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. MitoFit Preprint Arch. doi:10.26124/m

::: [[MitoEAGLE protocols, terminology, documentation |WG1: MitoEAGLE protocols, terminology, documentation]] ::::* [[Doerrier 2018 MiP2018a |Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers]]

::::* Integration of the new protocols in the DatLab software ...nd correction, experimental SUIT (substrate-uncoupler-inhibitor-titration) protocols and data analysis.

::: [[MitoEAGLE protocols, terminology, documentation |WG1: MitoEAGLE protocols, terminology, documentation]] ...19 MitoFit Preprint Arch EA| Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers]]

.... Moreover, such database will help in the harmonization of mt-respiratory protocols and the resolution of the reproducibility crisis in mt–research [2].

| Training in the Oroboros O2k-Laboratory – from SUIT protocols to interpretation and reporting |Harmonization of respirometric protocols comparing isolated mitochondria and permeabilized muscle fibers

...management network will be a strategic innovation to develop harmonization protocols towards generating a rigorously monitored data repository on mitochondrial ...y is required to implement quality control systems and optimally harmonize protocols across research groups. The primary challenge is to ‘increase value and r

| White adipose tissue mitochondrial respiration protocols - Experiments and Results discussion | Inter-laboratory harmonization of protocols to set a final consensus for the evaluation of the technical skills needed

...working with "mitochondria people", getting to know the Oxygraph, methods, protocols. I am well certain that soon I will be also a user of the Oroboros instrume :::: '''A1''': Diagnostic substrate-uncoupler-inhibitor titration (SUIT) protocols of mitochondrial fitness.

...ps://www.ncbi.nlm.nih.gov/pubmed/17406588 Frezza ''et al.'' (2007), Nature Protocols] ...Fighting against reproducibility crisis: Inter-laboratory harmonization of protocols for mitochondrial function evaluation in permeabilized muscle fibers. - [[M