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...mitochondrial elementary marker, ''mtE'') for normalization of respiratory flux. == Normalization of respiratory flux for CS as a mitochondrial marker ==

...d on internal normalization for rate in a reference state, are independent of externally measured markers and, therefore, are statistically robust. {{#ask:[[Additional label::Normalization of rate]]

...tEU). The [[motive unit]] [MU] of chemical flow or flux is the advancement of reaction [mol] in the chemical format. ...) for the '''Chamber''' volume. Normalization yields a specific quantity ''flux'' from the [[extensive quantity]] ''flow''. From [[Gnaiger 2019 MitoFit Pre

..._O2max, is less mass-dependent across a large range of body mass of different mammalian species (Weibel and Hoppeler 2005). ...) for the '''Chamber''' volume. Normalization yields a specific quantity ''flux'' from the [[extensive quantity]] ''flow''. From [[Gnaiger 2019 MitoFit Pre

...any form of '''flow''', ''I'' is the [[advancement]] of a process per unit of time, expressed in a specific motive unit [MU∙s^-1], ''e.g.'', ...e '''Chamber''' volume. Normalization yields the [[specific quantity]] ''[[flux]]'' from the [[extensive quantity]] ''flow''. From [[Gnaiger 2019 MitoFit P

...in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0 % and 100 %). ...n of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]].

...ed system. The magnitude of these quantities depends on the extent or size of the system ([[Cohen 2008 IUPAC Green Book |Cohen et al 2008]]). ...'''Sample''' (flow per object, mass-specific flux, or cell-volume-specific flux); or (2) for the '''Chamber''' volume. Flow per object and flow per system

...per mg protein or wet mass). These are different forms of [[normalization of rate]]. ...Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v2, [[Gnaiger 20

Version 04: New Section '''6. Normalization of respiratory flux for CS activity''' |keywords=Normalization of rate

...up>-1</sup>·x^-1]. These are different forms of [[normalization of rate]].

...lux, i.e. flux ''E'' in the ET-state, such that the lower and upper limits of the ''CCR'' are defined as 0.0 and 1.0. Then there are two mitochondrial '' |info=[[Flux control ratio]], [[Gnaiger 2020 BEC MitoPathways]]

:'''Protocol''': (read only) Displays the name of the DL8-Protocol selected (if applicable). :'''Sample type''': Enter the type of sample used in the experiment.

...r un-inhibited) flux; ''Y_X'' is the [[background state]] at low flux, upon which ''X'' acts. ...y of ''X'' upon background ''Y_X'' is expressed as the change of flux from ''Y_X'' to ''Z_X'' normalized for the reference st

...ab to [[Mark statistics - DatLab|Mark statistics]] (F2), select which type of marks you want to export ("All marks in plot" or "DL-Protocol marks", with == DatLab-Analysis templates for O2k-analysis of respiratory flux - strategy ==

...can be chosen to be displayed as Flux per volume or as normalized specific flux/flow, the background correction can be switched on or off, and the channel ...2k chamber B, in each case the first row is for the signal, the second for Flux/Slope.

...E consortium, following a primary peer-reviewed publication of the concept of stoichiometric potential differences. ...l oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux

...e.g. medians) are displayed for these time sections of the source plot and of all selected plots. ...asurements in a high oxygen regime or using samples with a low respiratory flux or flow.

:::: To benefit optimally from the tutored training sessions of the O2k-Virtual Support, please make yourself familiar with '''Materials fo '''Pathway control and coupling control of mitochondrial respiration:'''

...ol ratio]]s. » [[Mitochondrial marker#Mitochondrial markers and expression of mitochondrial respiration| '''MiPNet article''']] = Mitochondrial markers and expression of mitochondrial respiration =

...me ''V'' of 0.5 mL. The current DatLab version is included in the delivery of this revolutionary module. ...amilton syringe. Accurate kinetic titrations, however, require application of the TIP2k, with accurately placed needles extending slightly into the mixed

...sider the message in the selected keywords. Provide consistent definitions of your keywords by linking them to MitoPedia. Extend MitoPedia entries critic ::::## If yes, you may be satisfied with the definition of your keyword provided by MitoPedia - done.

...[flux smoothing]], selection of the [[scaling factor]], and stoichiometric normalization using a stoichiometric coefficient. ...asurements in a high oxygen regime or using samples with a low respiratory flux or flow.

...ntinuous system |vectorial]] transport processes are inherently non-linear flux-force relationships, with '''''L''''' = '''''u'''''·'''''c''''' in continu == Linear laws in transport processes are not linear flux-force laws ==

|title=Dysregulation of muscle ATP synthesis in hypophosphatemic mouse models and in a patient with ...henomenon remains unclear [1]. In this study we examine the potential role of decreased insulin-stimulated phosphate transport in this process.

High-resolution respirometry of adipose tissue biopsies and tracer determined whole body lipolysis. Sixteen ...een the oxidative phosphorylation system capacity (''P'') and the capacity of the electron transfer-pathway (''E'') increased 18 months after RYGB in bot

...rameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an '''O2k-MultiSensor''' application. The O2k fully su ...uorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluo

...en KF, Bergwitz C, Shulman GI (2016) Hypophosphatemia promotes lower rates of muscle ATP synthesis. FASEB J 30:3378-87. ...by low blood P_i concentrations, which may explain some aspects of muscle weakness observed in patients with hypophosphatemia.

...Oroboros for [[ASMRM 2018 Busan KR |MIRACLE]] - the bioenergetic snapshot of mitochondrial fitness ('''IOC135'''). ::: '''Basic and advanced applications of the Oroboros O2k-FluoRespirometer'''

...efer D, Gnaiger E (2011) Increased OXPHOS capacity after cold preservation of the human heart – a paradox resolved by a dyscoupling mechanism. MiP2011 ...ET capacity indicates a mitochondrial dysfunction after cold preservation of human heart tissue, which contrasts to mitochondrial injuries involved in i

...From OCR and ECAR to energy: perspectives on the design and interpretation of bioenergetics studies. J Biol Chem 207:101140. https://doi.org/10.1016/j.jb ...he number of studies exploring, in particular, the biological implications of oxygen consumption rate (OCR) and substrate level phosphorylation via glyco

...nts this notion with its company logo: the Ouroboros. The feedback process of the dragon feeding from its tail symbolizes immortality and can be translat ...knowledge on how to improve present standards achieved in the applications of [[high-resolution respirometry]], how the available technologies can be opt

::: <big>'''Building blocks of mitochondrial physiology'''</big> '''MitoEAGLE recommendations Part 1.''' ...ollow-up reports by the expanding MitoEAGLE Network, to increase the scope of consensus-oriented recommendations and facilitate global communication and

...'_''X'' of ''X'' and change of the amount d''n''_''X'' of ''X''; Eq. 8.3 | catabolic rate of respiration || [[Cell respiration]] || ''J''_kO₂; ''I'

...to review the state-of-the-art terminology in the rapidly expanding field of mitochondrial respiratory physiology. From the [[MitoPedia: Respiratory sta ...enhagen DK 2013|- "Theory & Praxis"]] - the annual highlight that students of cellular bioenergetics and mitochondrial physiology cannot afford to miss!

...tion and mitochondria associated protein expression in response to 2-weeks of high intensity interval training. Front Physiol 6:51. ...functional and metabolic adaptation in skeletal muscle, but the influence of HIT on mitochondrial function remains poorly studied in humans. Mitochondri

|title=The functional design of the electron transfer system (ETS) and mitochondrial respiratory control. ...TCA cycle and other mt-matrix dehydrogenase activities, and (iii) activity of the inner mt-membrane bound ETS (mETS) [1].

|title=[[Image:SumbalovaZ.JPG|left|90px|Zuzana Sumbalova]] Purity of blood cell fractions in respirometric studies: peripheral blood mononuclear ...ificantly contribute to the respiration typically expressed per cell count of the target cells.

...of SUIT protocols and fostering proficiency to generate experimental data of assured high quality as required for a database on mitochondrial physiology ...mbrane potential with fluorometric and potentiometric methods, measurement of respiration and acidification, quality control management in HRR. The O2k-C

...stromal cells injection improves rat liver regeneration through activation of mitochondrial functions after subtotal hepatectomy. ...in rat liver tissue after subtotal hepatectomy and to investigate effects of multipotent stromal cells (MSCs) injection on liver regeneration.

09:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k. 10:30 ''Break – Registration of external participants''

...bers from arm and leg muscle in Inuit versus Caucasians: A functional test of the uncoupling hypothesis in Greenland. Mitochondr Physiol Network 18.08. ...enland]] [[MiP2013]], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]], '''[[Gnaiger 2015 Scand J Med Sci Sports]]'''

...'·''U''_ce ([[Gnaiger MitoFit Preprints 2020.4]]). Normalization of respiratory rate by cell count yields oxygen [[flow]] ''I''<sub>O<sub>2</su ...y. The corresponding corrections, however, depend on the technical details of the procedures used when adding cell suspensions into the chamber with stop

|title=OXPHOS and ET capacity in permeabilized fibers of canine superathletes. ...bstrates. Both protocols were designed to provide a common reference state of NS-linked ET capacity, in comparison to separate N- and S-linked pathway co

[[File:Group photo.jpg|400px|right|A picture of the group]] ...nd Houses of Parliament]] [[File:Houses of Parliament.jpg|250px|The Palace of Westminster]] [[File:Cathedral.jpg|250px|St. Paul's Cathedral]] [[File:Brid

...r, normalization is mandatory for comparability of results. Concept-driven normalization and regression analysis are key towards bioenergetic cluster analysis prese ...followed by sequential titrations of oligomycin, uncoupler, and inhibitors of electron transfer.

:::* Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution ...OXPHOS protocols for human cells, permeabilized fibres from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782 (Palmeira CM, More

...]] Developing a method of assessing mitochondrial functions in homogenates of human heart muscle. ...icated and inaccurate determination of citrate synthase activity as marker of mitochondrial content in the measured sample.

|'''Did you know that you can set a warning alarm for the lower limit of the O₂ concentration in DatLab 7? '''<br> Yes, oxygen signals obtained after proper calibration are independent of the gain setting, and can therefore be grouped together for comparison and

...e mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes. ...bstrates. Both protocols were designed to provide a common reference state of CI&II-linked ET capacity, in comparison to separate Complex I- and Complex

...w.mitoglobal.org/index.php/MITOEAGLE|COST Action MitoEAGLE]] The reduction of mitochondrial functionality by a strawberry extract: a mechanism that contr ...AMP/ATP ratio, the objective of the present work was to evalute the effect of a strawberry extract on mitochondrial functionality in HepG2 cells.

...and ET-capacity in permeabilized fibers of canine superathletes. Analysis of HRR data presenting unexpected challenges. ...bstrates. Both protocols were designed to provide a common reference state of CI<small>&</small>II-linked ET-capacity, in comparison to separate Complex

...e mitochondrial physiology: OXPHOS and ET capacity in permeabilized fibers of canine superathletes. ...bstrates. Both protocols were designed to provide a common reference state of CI<small>&</small>II-linked ET capacity, in comparison to separate Complex

|title=Effects of inhibiting myosin-ATPase on mitochondrial respiratory capacity in permeabil ...therefore, be to introduce contraction inhibitor early in the preparation of the sample.

|abstract='''Abstracts are listed here in the frame of the [[MiPMap]] for the 7^th MiP''school'' on Mitochondrial Physio '''Organizer''': [[Ojuka EO|Edward Ojuka]] (University of Cape Town, South Africa), The MiP''society''.

:::: '''<big><big>Canon O</big>: Assembly of Canonical Carol Canons on the Oroboros Ecosystem</big>''' ...nd how you are, when entering the Canoncal '''O'''2k-Manual in the jukebox of the Canonical Carol.

...PNet Reference Laboratories]] Quality assurance in respirometric diagnosis of mitochondrial function. MiPNet Workshop 9-10 Dec 2009. Mitochondr Physiol N ...f aged humans versus young controls using biopsies, or (iii) the diagnosis of a mt-myopathy in a single patient when an individual is evaluated (she/he r

...unction in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, educatio ...l oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux, O₂

'''Number of participants:''' Max. 75 participants ...ilities at the East Carolina Heart Institute on the health sciences campus of East Carolina University.

...F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Act ...atcliffe P, Baes M, Maxwell P, Carmeliet P (2008) Deficiency or inhibition of oxygen sensor Phd1 induces hypoxia tolerance by reprogramming basal metabol

...x|right|thumb|Fig. 1. Workflow in the pre-analytical and analytical phases of blood cell preparation and respiration in living cells (ce) and permeabiliz 3.4. Exclusion criteria derived from haematology of whole blood and plasma

...ogo_Univ_Innsbruck.jpg|100px|right|link=https://www.uibk.ac.at |University of Innsbruck]] ...www.mitoeagle.org/index.php/MitoEAGLE_preprint_2018-02-08 |Building blocks of mitochondrial physiology]]

...ory states |Respiratory states]], [[MitoPedia: Respiratory control ratios |Flux control ratios]] ::: '''Preface''' - the 2014 edition of the 'Blue Book' was released at [[MiP2014]] and [[IOC95]].

Did you know that keeping your mitochondria fit is essential for quality of life, brain and muscle function, and resistance against preventable, immuno ...c leads to multimorbidity in aging and threatens to overwhelm the capacity of healthcare systems.

...nce of workshops rather than a conference or school, addressing key topics of the COST Action [[MitoEAGLE]] project. This will provide a key opportunity ...s own risk. Sport programme is offered in collaboration with the Institute of Sport Science (University Innsbruck), Sporttherapie Huber, and the High Alt

Coenzyme Q is a lipid composed of a benzoquinone ring with an isoprenoid side chain, two methoxy groups and ...ch is controlled by cellular energy demand, energy turnover and the degree of coupling to phosphorylation. <br>

=== A1: Diagnostic SUIT protocols of mitochondrial fitness === ...protocols will rigorously document (Maelstrom Research program) the state-of-the-art in designing, conducting, reporting, interpreting, and validating s

...:00 [[Schmitt_S|Sabine Schmitt]]: "Determination of hydrogen peroxide flux of isolated mitochondria from mouse embryonic fibroblasts induced by mitochond ::::* 16:00-16:30 [[Stefan_E|Eduard Stefan]]: “Decoding and Perturbation of ONCO-Kinase functions”

...unction in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, educatio ...l oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux, O₂

|'''Antioxidant supplementation: Effects of MitoQ and CoQ10 on ROS production and mitochondrial function'''<br> Yes, oxygen signals obtained after proper calibration are independent of the gain setting, and can therefore be grouped together for comparison and

...unction in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, educatio ...orce]] • [[Residual oxygen consumption]] • [[SI - The International System of Units]] • [[Uncoupling]]

...even at the penultimate level of formalization in the International System of Units (SI). ...besity pandemic, challenge MAWIA medicine, global warming, and destruction of biological and cultural diversity, where MAWIA plays a MAW-dominant (Male-A

...ntists to grow their ideas by sharing them with their peers. The objective of COST Action CA15203 MitoEAGLE (http://www.mitoglobal.org/index.php/MitoEAGL === Memorandum of Understanding ===