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- Baglivo 2022b ESCI Bari + (Evaluation of instrumental reproducibility โฆ Evaluation of instrumental reproducibility is an essential component of quality control ฬถ defined as standard operating procedures ฬถ to quantify the precision and limit of detection of an analytical procedure.</br></br>Instrumental tests implemented as standard operating procedures in high-resolution respirometry are the sensor test and the chamber test. The sensor test includes calibrations of the signal of the polarographic oxygen sensor (POS) in terms of oxygen concentration cO<sub>2</sub> [ยตM] to evaluate the performance of the POS. The chamber test (instrumental O<sub>2</sub> background test) focuses on the slope dcO<sub>2</sub>/dt to determine oxygen consumption by the POS and backdiffusion into the chamber [1]. We evaluated instrumental tests of 48 Oroboros O2k chambers obtained from a 3-year study on MiR05-Kit (Oroboros Instruments), carried out in the absence of sample. </br></br>Stability of oxygen calibration signals at air saturation and zero oxygen was monitored up to 8 months. The maximum drift over 1 to 3 days was 0.05 pmolโs<sup>โ1</sup>โmL<sup>โ1</sup>, with no persistence over time since drift was < 0.004 pmolโs<sup>โ1</sup>โmL<sup>โ1</sup> for a time interval of one month, corresponding to a drift per day of 0.2 % of the signal at air saturation. Instrumental O<sub>2</sub> background dcO<sub>2</sub>/dt was stable within ยฑ1 pmolโs<sup>โ1</sup>โmL<sup>โ1</sup> at different O<sub>2</sub> concentrations when measured at monthly intervals. </br></br>Taken together, these results confirm the instrumental limit of detection of volume-specific O<sub>2</sub> flux at ยฑ1 pmolโs<sup>โ1</sup>โmL<sup>โ1</sup>. Following the standard operating procedures applied in the present study provides an instrumental proficiency test to ensure the unique reproducibility in high-resolution respirometry.lt;/sup>โmL<sup>โ1</sup>. Following the standard operating procedures applied in the present study provides an instrumental proficiency test to ensure the unique reproducibility in high-resolution respirometry.)