Step
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State
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Pathway
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Q-junction
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Comment - Events (E) and Marks (M)
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0DTPA
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- DTPA is an iron chelator, which decreases the chemical fluorescence background created by the Amplex UltraRed assay. Administration of DTPA into the O2k-chamber is recommended before all other chemicals because the iron chelation capacity of the compound is time-dependent (approx. 10-15 min). However, the experiments can be carried out in the absence of DTPA.
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0SOD
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- SOD or superoxide dismutase converts the anion superoxide released by the mitochondria into H2O2, making it accessible to the Amplex UltraRed assay.
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0HRP
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- HRP or horseradish peroxidase catalyses the conversion of Amplex UltraRed and H2O2 towards the fluorescent resorufin.
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0DMSO
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- DMSO is a solvent that dissolves both polar and nonpolar compounds and is miscible in a wide range of organic solvents as well as water. DSMO titration into the O2k-chamber is used to study the effect of the carrier titrations on the respiration.
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