Template:SUIT-002: Difference between revisions

Revision as of 14:44, 11 January 2019

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
1D	ROX			ADP is added to stimulate consumption of endogenous fuel-substrates. Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration.
2M.1
3Oct	Oct_P	(F)	FAO	Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration.
3c	OctMc_P	F	FAO	Respiratory stimulation of the FAO-pathway, F, by fatty acid, FA, in the presence of malate, M. Malate is a type N substrate (N), required for the F-pathway. The FA concentration has to be optimized to saturate the F-pathway, without inhibiting or uncoupling respiration. OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]).
4M2	OctM_P	F	FAO
5P	OctPM_P	FN	FAO&CI
6G	OctPGM_P	FN	FAO&CI
7S	OctPGMS_P	FNS	FAO&CI&II
8Gp	OctPGMSGp_P	FNSGp	FAO&CI&II&GpDH
9U	OctPGMSGp_E	FNSGp	FAO&CI&II&GpDH
10Rot	SGp_E	SGp	CII&GpDH
11Ama	ROX

Step	Respiratory state	Pathway control	ET-Complex	Comment
## AsTm	AsTm_E	CIV	CIV
## Azd	CHB

@@ Line 1: / Line 1: @@
 [[File:1D;1M.1;2Oct;2c;2M2;3P;4G;5S;6Gp;7U;8Rot;9Ama;10Tm;11Azd.jpg|700px|link=SUIT-RP2 |SUIT-RP2]]
 {| class="wikitable" border="1"
-|-
+{{Template:SUIT header}}
-! Step
-! Respiratory state
-! Pathway control
-! Pathway to Q
 |-
 | 1D
+| [[ROX]]
 |
-| [[ROX]]
+|
-| ROX
+| ADP is added to stimulate consumption of endogenous fuel-substrates.
+{{Template:SUIT ROX}}
 |-
@@ Line 21: / Line 19: @@
 |-
 | 3Oct
-| [[Oct]](P)
+| [[Oct]]<sub>''P''</sub>
 | ([[F]])
 | FAO
+| {{Template:SUIT F}}
 |-
 | 3c
-| [[OctM]](P)c
+| [[OctM]]c<sub>''P''</sub>
 | [[F]]
 | FAO
+| {{Template:SUIT F}} {{Template:SUIT OXPHOS}} {{Template:SUIT c}}
 |-
 | 4M2
-| [[OctM]](P)
+| [[OctM]]<sub>''P''</sub>
 | [[F]]
 | FAO
@@ Line 39: / Line 39: @@
 |-
 | 5P
-| [[OctPM]](P)
+| [[OctPM]]<sub>''P''</sub>
 | [[FN]]
 | FAO&CI
@@ Line 45: / Line 45: @@
 |-
 | 6G
-| [[OctPGM]](P)
+| [[OctPGM]]<sub>''P''</sub>
 | [[FN]]
 | FAO&CI
@@ Line 51: / Line 51: @@
 |-
 | 7S
-| [[OctPGMS]](P)
+| [[OctPGMS]]<sub>''P''</sub>
 | [[FNS]]
 | FAO&CI&II
@@ Line 57: / Line 57: @@
 |-
 | 8Gp
-| [[OctPGMSGp]](P)
+| [[OctPGMSGp]]<sub>''P''</sub>
 | [[FNSGp]]
 | FAO&CI&II&GpDH
@@ Line 63: / Line 63: @@
 |-
 | 9U
-| [[OctPGMSGp]](E)
+| [[OctPGMSGp]]<sub>''E''</sub>
 | [[FNSGp]]
 | FAO&CI&II&GpDH
@@ Line 69: / Line 69: @@
 |-
 | 10Rot
-| [[SGp]](E)
+| [[SGp]]<sub>''E''</sub>
 | [[SGp]]
 | CII&GpDH
@@ Line 75: / Line 75: @@
 |-
 | 11Ama
-|
-| ROX
-| ROX
-|-
-| 12Tm
-| [[Tm]](E)
-| [[CIV]]
-| CIV
-|-
-| 13Azd
-|
-| ROX
-| ROX
-|}
-[[SUIT-011]]
-{| class="wikitable" border="1"
-|-
-! Step
-! Respiratory state
-! Pathway control
-! ET-Complex entry into Q-junction
-! Comment
-|-
-| 1GM
-| [[GM]]<sub>''L''</sub>
-| [[N]]
-| CI
-| GM<sub>''L''</sub> or GM_L: N-LEAK respiration, N<sub>''L''</sub>
-NADH-linked substrates glutamate & malate (type N; CI-linked pathway to Q). Non-phosphorylating resting state (LEAK state); ''L''<sub>n</sub> in the absence of ADP, ATP, AMP (no adenylates).
-|-
-| 2D
-| [[GM]]<sub>''P''</sub>
-| [[N]]
-| CI
-| GM<sub>''P''</sub> or GM_P: N-OXPHOS capacity, N<sub>''P''</sub>
-OXPHOS capacity, ''P'' (with saturating [ADP]), with NADH-linked substrates glutamate&malate.
-|-
-| D(c)
-| [[GM]]c<sub>''P''</sub>
-| [[N]]
-| CI
-| GMc<sub>''P''</sub> or GMc_P: Cytochrome c test for quality control
-Addition of cytochrome ''c'' yields a test for integrity of the [[mtOM]]. Stimulation by added cytochrome ''c'' would indicate an injury of the mtOM and limitation of respiration in state GM<sub>''P''</sub> due to loss of cytochrome ''c'' (''P'', OXPHOS capacity with type N substrates). Cytochrome ''c'' is added immediately after the earliest ADP-activation step.
-|-
-| 3S
-| [[GMS]]<sub>''P''</sub>
-| [[NS]]
-| CI&II
-| GMS<sub>''P''</sub> or GMS_P: NS-OXPHOS capacity, NS<sub>''P''</sub>
-Respiratory stimulation by further addition of succinate, S, to type N substrates, with convergent electron flow in the NS-pathway (CI<small>&</small>II-linked pathway to the Q-junction) for reconstitution of TCA cycle function, in the coupled state as an estimate of OXPHOS-capacity, ''P''.
-|-
-| 4U
-| [[GMS]]<sub>''E''</sub>
-| [[NS]]
-| CI&II
-| GMS<sub>''E''</sub> or GMS_E: NS-ET capacity, NS<sub>''E''</sub>
-Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity (noncoupled ET-state), as a test for limitation of OXPHOS-capacity by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET-capacity, E.
-|-
-| 5Rot
-| [[S]]<sub>''E''</sub>
-| [[S]]
-| CII
-| S<sub>''E''</sub> or S_E: S-ET capacity
-S-pathway ET-capacity after blocking CI with rotenone.
-|-
-| 6Ama
 | [[ROX]]
 |
 |
-| ROX: residual oxygen consumption
-''Rox'' is due to oxidative side reactions, estimated after addition of Antimycin A (inhibitor of CIII). ''Rox'' is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
 |}
+{{Template:SUIT CIV}}