https://wiki.oroboros.at/api.php?action=feedcontributions&user=Antunes+Diana&feedformat=atom
Bioblast - User contributions [en]
2024-03-19T11:19:22Z
User contributions
MediaWiki 1.36.1
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206550
Template talk:Next oroboros ecosystem agenda
2020-08-28T08:42:01Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr''' (pending publication of Luiza's video communication)<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
===='''Author names of O2k-Publications/ Brief for the Agenda vs Bioblast''' ==== <br />
::1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: [https://wiki.oroboros.at/index.php/File:TEMPLATE_ALERT.png Template alerts].<br />
:::Example: [https://wiki.oroboros.at/index.php/O2k-Publications_alert Oroboros Ecosystem Agenda example]<br />
:::This means that '''we have to edit manually the author names''' within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.<br />
::2.Bioblast: SOP: [https://www.bioblast.at/index.php/Form:Publication] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.<br />
<br />
__NOTOC__ [[File:Expand.png|right|25px|Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
'''Guidelines to update a reference'''<br />
<div class="mw-collapsible-content"><br />
::When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:<br />
[[File:Reference example.png|900px]]<br />
::Correct format for a person called “First Name Surname”: <br />
::In the gray box should be “Surname FN” - '''From where references to be used as O2k-Publication/Brief alerts are copied from.'''<br />
[[File:Gray box reference.png|900px]]<br />
::In the links should be “ Surname First N”<br />
[[File:Authors names links reference.png|900px]]<br />
::'''How to edit:'''<br />
:::A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.<br />
:::B) Click in “Edit with form”.<br />
:::C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.<br />
[[File:Editing reference.png|900px]]<br />
<br />
'''How to move a profile (instructions sent by Marija):'''<br />
<br />
So let’s take for example my page: <br />
[[Beno M]] <br />
[https://wiki.oroboros.at/index.php?title=Beno_Marija&redirect=no]<br />
<br />
::As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps: <br />
:::1. Go to e.g. “Beno M” on edit<br />
:::2. Click on “Move”<br />
:::3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija <br />
[[File:BenoMreference.jpg|500px]]<br />
:::4. Now it should look like that: <br />
[[File:BenoMarijaReference.png|500px]]<br />
:::5. Click on “Move” and leave all the ticked boxes<br />
:::6.Done<br />
::After that you can continue in step B.<br />
::If you have any doubt you can contact Cristiane, Marija or Mario.<br />
<br />
<br />
</div><br />
</div><br />
=== Next ===<br />
<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206470
Template talk:Next oroboros ecosystem agenda
2020-08-26T12:22:28Z
<p>Antunes Diana: /* Author names of O2k-Publications/ Brief for the Agenda (alerts) vs Bioblast */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
===='''Author names of O2k-Publications/ Brief for the Agenda vs Bioblast''' ==== <br />
::1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: [https://wiki.oroboros.at/index.php/File:TEMPLATE_ALERT.png Template alerts] under [https://wiki.oroboros.at/index.php/Template_talk:Next_oroboros_ecosystem_agenda Oroboros Ecosystem Agenda - Next].<br />
:::Example: [https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=195966&oldid=195045 Oroboros Ecosystem Agenda example]<br />
:::This means that '''we have to edit manually the author names''' within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries))<br />
::2.Bioblast: SOP: [https://www.bioblast.at/index.php/Form:Publication] – examples are available using both Surname and First name with long version (e.g., Antunes Diana)<br />
<br />
=== Next ===<br />
<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206469
Template talk:Next oroboros ecosystem agenda
2020-08-26T12:19:54Z
<p>Antunes Diana: /* O2k-Publications alert */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
===='''Author names of O2k-Publications/ Brief for the Agenda (alerts) vs Bioblast''' ==== <br />
::1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: [https://wiki.oroboros.at/index.php/File:TEMPLATE_ALERT.png Template alerts] under [https://wiki.oroboros.at/index.php/Template_talk:Next_oroboros_ecosystem_agenda Oroboros Ecosystem Agenda - Next].<br />
:::Example: [https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=195966&oldid=195045 Oroboros Ecosystem Agenda example]<br />
:::This means that '''we have to delete manually the author names''' (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries))<br />
::2.Bioblast: SOP: [https://www.bioblast.at/index.php/Form:Publication] – examples are available using both Surname and First name with long version (e.g., Antunes Diana)<br />
=== Next ===<br />
<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206468
Template talk:Next oroboros ecosystem agenda
2020-08-26T12:19:37Z
<p>Antunes Diana: /* Author names of O2k-Publications/ Brief for the Agenda (alerts) vs Bioblast */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206467
Template talk:Next oroboros ecosystem agenda
2020-08-26T12:18:56Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
<br />
=='''Author names of O2k-Publications/ Brief for the Agenda (alerts) vs Bioblast''' == <br />
::1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: [https://wiki.oroboros.at/index.php/File:TEMPLATE_ALERT.png Template alerts] under [https://wiki.oroboros.at/index.php/Template_talk:Next_oroboros_ecosystem_agenda Oroboros Ecosystem Agenda - Next].<br />
:::Example: [https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=195966&oldid=195045 Oroboros Ecosystem Agenda example]<br />
:::This means that '''we have to delete manually the author names''' (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries))<br />
::2.Bioblast: SOP: [https://www.bioblast.at/index.php/Form:Publication] – examples are available using both Surname and First name with long version (e.g., Antunes Diana)<br />
<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206465
Template talk:Next oroboros ecosystem agenda
2020-08-26T12:17:03Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
<br />
*'''Author names of O2k-Publications/ Brief for the Agenda (alerts) vs Bioblast''' <br />
::1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: [https://wiki.oroboros.at/index.php/File:TEMPLATE_ALERT.png Template alerts] under [https://wiki.oroboros.at/index.php/Template_talk:Next_oroboros_ecosystem_agenda Oroboros Ecosystem Agenda - Next].<br />
:::Example: [https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=195966&oldid=195045 Oroboros Ecosystem Agenda example]<br />
:::This means that '''we have to delete manually the author names''' (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries))<br />
::2.Bioblast: SOP: [https://www.bioblast.at/index.php/Form:Publication] – examples are available using both Surname and First name with long version (e.g., Antunes Diana)<br />
<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206447
Template talk:Next oroboros ecosystem agenda
2020-08-26T10:04:34Z
<p>Antunes Diana: /* O2k-Open Support alert circular 2020(07): in prep */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206444
Template talk:Next oroboros ecosystem agenda
2020-08-26T09:45:44Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Sep-04 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Sep-01 Tu'''<br />
<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Complex I dysfunction induced by N-methyl carbamate can be alleviated with a cell-permeable succinate prodrug'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]<br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-OpenSupportAlert2020_Archive&diff=206442
O2k-OpenSupportAlert2020 Archive
2020-08-26T07:30:23Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
<br />
<br />
== Aug 2020 ==<br />
* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br><br />
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]«<br />
|}<br />
<br />
<br />
== Previous O2k-Open Support Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
<br />
<br />
== Jul 2020 ==<br />
<br />
* '''Jul-27 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''<br />
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br><br />
- » [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«<br />
|} <br />
----<br />
<br />
* '''Jul-20 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''How can you compare respirometric experiments using an internal normalization?'''<br><br />
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”. <br />
<br><br />
- »[[Flux control ratio]]«<br />
<br />
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
----<br />
* '''Jul-13 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br><br />
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. <br />
For more information, see:<br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP Copy from file]«<br />
|}<br />
----<br />
<br />
* '''Jul-06 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br><br />
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear. <br />
For more information, see:<br />
- »[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]« and »[[DatLab error messages]] «<br />
|}<br />
<br />
== Jun 2020 ==<br />
<br />
* '''Jun-01 Mo''' <br />
:{|<br />
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«<br />
|}<br />
----<br />
* '''Jun-15 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
<br />
----<br />
<br />
* '''Jun-22 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Did you know a stirrer test can save your experiments?'''<br><br />
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
* '''Jun-29 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br><br />
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br><br />
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«<br />
|}<br />
=== May 2020 ===<br />
<br />
* '''May-25 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br><br />
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br><br />
- »[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«<br />
|}<br />
<br />
* '''May-18 Mo''' <br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br><br />
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br><br />
- »[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«<br />
|}<br />
<br />
* '''May-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|''' Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.''' <br><br />
Learn more about steady states including performance and mark setting for data analysis. <br><br />
- »[[Steady state]]«<br />
|}<br />
<br><br />
* '''May-04 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you load the correct instrumental background correction according to the actual of experimental O<sub>2</sub> concentrations?'''<br><br />
Perform and apply the instrumental background test.<br><br />
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«<br />
|}<br />
=== Apr 2020 ===<br />
* '''Apr-27 Mo'''<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''<br><br />
The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub> concentrations.<br><br />
- »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«<br />
|}<br />
<br />
<br><br />
* '''Apr-20 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Improve]]<br />
|'''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''<br><br />
Unstable respiratory fluxes in OXPHOS might be due to substrates limitation.<br><br />
- »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«<br />
|}<br />
<br><br />
* '''Apr-14 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Why are the oxygen levels different between chambers prior to my experimental run?'''<br><br />
After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations.<br><br />
- »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«<br />
|}<br />
<br><br />
* '''Apr-07 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|70px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]]<br />
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''<br><br />
If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]].<br><br />
- »[[SUIT: Browse DL-Protocols and templates]]«<br />
|}<br />
<br><br />
* '''Apr-02 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|70px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]][[File:Blue-info.png|25px|Improve]]<br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?'''<br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-30 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:'''<br><br />
- »'''[[Enable DL-Protocol editing]]'''«<br />
|}<br />
<br><br />
* '''Mar-25 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4:<br><br />
- »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br />
|}<br />
<br><br />
* '''Mar-23 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''<br><br />
'''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br><br />
'''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''<br />
While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration.<br><br />
- »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«<br />
|}<br />
<br><br />
* '''Mar-04 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
| [[File:OroboPOS-Holder002.jpg|100px]]<br />
| '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''<br />
See how you can check that the two blue OroboPOS-Holders are intact.<br><br />
- »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-28 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Calibration parameters are transferred to experimental DatLab files'''<br />
Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.<br><br />
- »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-31 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''<br />
In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP).<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br><br />
* '''Jan-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''<br />
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample.<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you set mark ‘R1’ and complete the air calibration correctly?'''<br />
Your air calibration is not effective until you set mark ‘R1’ and click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window.<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br><br />
*'''Jan-24 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''<br />
Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed.<br><br />
- »[[O2k configuration]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
| '''Do you perform O2k-Chamber volume calibration?'''<br />
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]).<br><br />
- »[[O2k-Chamber MitoPedia]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?'''<br />
The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration.<br><br />
- »[[Oxygen solubility factor]]«<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPediaAlert2020_Archive&diff=206441
MitoPediaAlert2020 Archive
2020-08-26T07:29:36Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
== Aug 2020 ==<br />
* '''Aug-26 We''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]<br />
| '''Non-mitochondrial respiration or [[residual oxygen consumption]] (''Rox'')? — Concept-driven constructive terminology frames our perception.''' <br />
After inhibition of electron-transfer (ET) pathways or in the absence of fuel substrates, both ''mitochondrial'' non-ET reactions and ''non-mitochondrial'' reactions contribute to ''residual oxygen consumption'' in mt-preparations and living cells. <br><br />
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''« — Table 1: ''Rox'' induced by non-ET pathway oxidation reactions<br />
|}<br />
----<br />
<br />
* '''Aug-24 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]<br />
|'''Can NADH be used as an externally added substrate to obtain the [[NADH electron transfer-pathway state]] in [[Mitochondrial_respiration |mitochondrial respiration]]? '''<br><br />
No, in most types of intact mitochondria NADH cannot pass the [[mitochondrial inner membrane]] (mtIM). Yeast mitochondria are an exception. <br><br />
- »[[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide]]« and »[[MitoPedia:_Substrates_and_metabolites|Substrates and metabolites]]« <br />
|}<br />
----<br />
* '''Aug-20 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to with the new Q-Module being developed as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
----<br />
* '''Aug-13 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Photosynthesis efficiency is reduced by 25% in plants and algae due the metabolic process called photorespiration. <br><br />
- »'''[[Photorespiration]]'''«<br />
|}<br />
----<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|}<br />
<br />
<br />
== Previous MitoPedia Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
----<br />
* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
----<br />
<br />
* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
----<br />
* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
----<br />
* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
<br />
== Jun 2020 ==<br />
* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
<br />
* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
<br />
* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_Red_method Incompatible substrates]'''«<br />
|}<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
<br />
* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
<br />
<br />
=== May 2020 ===<br />
<br />
* '''May-28 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
<br />
* '''May-21 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
|As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
* '''May-14 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
<br />
* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''<br />
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br><br />
- Learn more about - »'''[[Malate-anaplerotic pathway control state|malate-anaplerotic pathway control state]]'''« <br><br />
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« <br><br />
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
=== Apr 2020 ===<br />
<br />
* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]]. <br><br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
<br><br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. <br><br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
<br><br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br><br />
- »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
<br><br />
* '''Apr-08 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. <br><br />
- »'''[[SUIT-010 |SUIT-010]]'''«<br />
|}<br />
<br><br />
* '''Apr-02 Th''' <br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' <br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-16 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. <br><br />
- »'''[[SUIT-003 |SUIT-003]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. <br><br />
- »'''[[Blebbistatin]]'''«<br />
|}<br />
<br><br />
* '''Mar-06 Fr'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br><br />
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? <br><br />
- »'''[[Rapamycin]]'''«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-26 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. <br><br />
- »'''[[Noncoupled respiration]]'''«<br />
|}<br />
<br><br />
* '''Feb-24 Mo'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| More research is needed on the impact of viral infection on mitochondrial function. <br><br />
- »'''[[Viruses and mitochondrial medicine]]'''«<br />
|}<br />
<br><br />
* '''Feb-19 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. <br><br />
- »'''[[Spermidine]]'''«<br />
|}<br />
<br><br />
* '''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Why do we fight gas bubbles? <br><br />
- Dive deep into »'''[[Oxygen solubility]]'''«<br />
|}<br />
<br><br />
* '''Feb-12 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| What do metformin, melatonin and other bioactive mitObesity compounds have in common? <br><br />
- »'''[[MitObesity drugs]]'''«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-16 Th'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Oxygen flux]]''' <br><br />
- which SI units should be used?<br />
|}<br />
<br><br />
<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Did you ever think that units for body mass could be an issue? <br><br />
- »'''[[Body mass]]'''«<br />
|}<br />
<br><br />
* '''Jan-08 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Next_oroboros_ecosystem_agenda&diff=206281
Template:Next oroboros ecosystem agenda
2020-08-20T06:13:15Z
<p>Antunes Diana: </p>
<hr />
<div><br />
<br />
2020 Week 35<br />
<br />
* '''Aug-24 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Can NADH be directly used as substrate in high-resolution respirometry (HRR)? '''<br><br />
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for [[Mitochondrial_respiration |mitochondrial respiration]] since the [[mitochondrial inner membrane]], which should be intact for HRR experiments, is not permeable to NADH. <br><br />
- »[[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide]]« and »[[MitoPedia:_Substrates_and_metabolites|Substrates and metabolites]]« <br />
|}<br />
----<br />
* '''Aug-25 Tu'''<br />
<br />
----<br />
* '''Aug-26 We''' <br />
<br />
----<br />
* '''Aug-27 Th''' <br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
----<br />
* '''Aug-28 Fr'''<br />
<br />
2020 Week 36<br />
<br />
* '''Aug-31 Mo''' <br />
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* '''Sep-01 Tu'''<br />
<br />
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----<br />
* '''Sep-02 We'''<br />
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----<br />
* '''Sep-03 Th'''<br />
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----<br />
* '''Sep-04 Fr'''<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
----<br />
<br />
* '''Sep-05 Sa''' - [[Bioenergetics Vienna 2020]]<br />
----<br />
* '''Sep-06 So''' <br />
::::* Abstract submission deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
----<br />
2020 Week 37<br />
* '''Sep-07 Mo''' <br />
::::* 19:00 / 20:15 '''MEGA Bildungsshow''' - 1 Million für Österreichs Talente. MARX HALLE Wien, Karl-Farkas-Gasse 19, 1030 Wien. - »[https://www.puls4.com/4GAMECHANGERS 4GAMECHANGERS]«<br />
<br />
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* '''Sep-08 Tu'''<br />
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----<br />
* '''Sep-09 We'''<br />
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* '''Sep-10 Th'''<br />
<br />
<br />
:::: »<big>'''[[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]]'''</big>« (Sep 10-12)<br />
<br />
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* '''Sep-11 Fr'''<br />
<br />
<br />
2020 Week 38<br />
<br />
* '''Sep-14 Mo''' <br />
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* '''Sep-15 Tu'''<br />
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* '''Sep-16 We'''<br />
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* '''Sep-17 Th'''<br />
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* '''Sep-18 Fr'''<br />
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* '''Sep-19 Sa''' <br />
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* '''Sep-20 So''' <br />
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2020 Week 39<br />
<br />
* '''Sep-21 Mo''' <br />
:::: [[File:Oegmbt.png|100px|link=https://wiki.oroboros.at/index.php/12th_%C3%96GMBT_Annual_Meeting_2020_Graz_AT|ÖGMBT Graz AT]] <big>'''[[12th ÖGMBT Annual Meeting 2020 Graz AT]]'''</big> Sep 21-23<br />
:::: <br />
----<br />
* '''Sep-22 Tu''' - [[12th ÖGMBT Annual Meeting 2020 Graz AT]] <br />
<br />
----<br />
* '''Sep-23 We''' - [[12th ÖGMBT Annual Meeting 2020 Graz AT]] <br />
<br />
----<br />
* '''Sep-24 Th'''<br />
::::* 18:00 - Houska Awards 2020 Vienna - [https://www.bcholding.at/houskapreis-1 Houskapreis der B&C Privatstiftung - Verleihung des Houskapreises 2020 in Wien]<br />
<br />
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* '''Sep-25 Fr'''<br />
<br />
2020 Week 40<br />
<br />
* '''Sep-28 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
----<br />
* '''Sep-29 Tu''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
----<br />
* '''Sep-30 We''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«<br />
::::* Early registration deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
----<br />
* '''Oct-01 Th''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
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* '''Oct-02 Fr''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
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* '''Oct-03 Sa''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
2020 Week 41<br />
<br />
* '''Oct-05 Mo''' <br />
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* '''Oct-06 Tu'''<br />
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* '''Oct-07 We'''<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] Pre-Conference O2k-Workshop »<big>'''[[MiPNet25.11 IOC148 Riga LV]] </big>'''«<br />
<br />
::::[[File:EU-CARDIOPROTECTION.png|100px|EU-CARDIOPROTECTION|link=6th EU-Cardioprotection Meeting 2020 Riga LV]] »<big>'''[[6th EU-Cardioprotection Meeting 2020 Riga LV]]'''</big>« (Oct 7-9)<br />
<br />
----<br />
* '''Oct-08 Th''' - [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
<br />
----<br />
* '''Oct-09 Fr''' - [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
:::: »<big>'''[[Long Night of Research 2020 Innsbruck AT]]'''</big>«<br />
<br />
2020 Week 42<br />
<br />
* '''Oct-12 Mo''' <br />
::::[[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Riga LV]]'''</big>« (Oct 7-9)<br />
----<br />
* '''Oct-13 Tu'''<br />
::::[[FAT4BRAIN School IOC147 Riga LV]]<br />
----<br />
* '''Oct-14 We'''<br />
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* '''Oct-15 Th'''<br />
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* '''Oct-16 Fr'''<br />
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* '''Oct-18 Su'''<br />
:::: »<big>'''[[ISCaM 2020 London UK]]'''</big>«<br />
<br />
2020 Week 43<br />
<br />
* '''Oct-19 Mo''' - [[ISCaM 2020 London UK]]<br />
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* '''Oct-20 Tu''' - [[ISCaM 2020 London UK]]<br />
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* '''Oct-21 We'''<br />
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* '''Oct-22 Th'''<br />
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* '''Oct-23 Fr'''<br />
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2020 Week 44<br />
<br />
* '''Oct-26 Mo''' <br />
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* '''Oct-27 Tu'''<br />
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* '''Oct-28 We'''<br />
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* '''Oct-29 Th'''<br />
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* '''Oct-30 Fr'''<br />
<br />
2020 Week 45<br />
<br />
* '''Nov-02 Mo''' <br />
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* '''Nov-03 Tu'''<br />
<br />
----<br />
* '''Nov-04 We'''<br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] <big>'''[[MitoEAGLE Summit Obergurgl 2020]]'''</big><br />
----<br />
* '''Nov-05 Th''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
----<br />
* '''Nov-06 Fr''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
* '''Nov-07 Sa''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
2020 Week 46<br />
<br />
* '''Nov-09 Mo''' <br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] <big>'''[[MiPschool Obergurgl 2020]]'''</big> including '''[[TRANSMIT School 2020]]'''<br />
----<br />
* '''Nov-10 Tu''' - [[MiPschool Obergurgl 2020]] including [[TRANSMIT School 2020]]<br />
<br />
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* '''Nov-11 We''' - [[MiPschool Obergurgl 2020]] including [[TRANSMIT School 2020]]<br />
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* '''Nov-12 Th'''<br />
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* '''Nov-13 Fr'''<br />
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2020 Week 47<br />
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* '''Nov-16 Mo''' <br />
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* '''Nov-17 Tu'''<br />
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* '''Nov-18 We''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
<br />
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* '''Nov-19 Th''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
<br />
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* '''Nov-20 Fr''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
<br />
2020 Week 48<br />
<br />
* '''Nov-23 Mo''' <br />
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* '''Nov-24 Tu'''<br />
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* '''Nov-25 We'''<br />
::::» <big>'''[[2020Spring PaduaMuscleDays Padova IT]]'''</big> / [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.04 IOC Padua IT |<big>'''IOC Padua IT'''</big>]] - ''separate registration''<br />
----<br />
* '''Nov-26 Th''' <br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
----<br />
* '''Nov-27 Fr'''<br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
----<br />
* '''Nov-28 Sa'''<br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
<br />
2020 Week 49<br />
<br />
* '''Nov-30 Mo''' <br />
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* '''Dec-01 Tu'''<br />
:::: [[Image:AlgaeEurope.jpg|60px|AlgaeEurope]] <big>'''[[AlgaEurope 2020 Rome IT]]'''</big><br />
<br />
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* '''Dec-02 We'''<br />
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* '''Dec-03 Th'''<br />
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* '''Dec-04 Fr'''<br />
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* '''Dec-05 Sa'''<br />
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* '''Dec-06 So'''<br />
::::* Registration deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
----<br />
2020 Week 50<br />
<br />
* '''Dec-07 Mo''' <br />
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* '''Dec-08 Tu'''<br />
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* '''Dec-09 We'''<br />
::::[[File:ESSR2020.png|100px|ESSR2020|link=https://wiki.oroboros.at/index.php/ESSR_2020_Innsbruck_AT]] <big>'''[[ESSR 2020 Innsbruck AT]]'''</big> (Dec 9-11)<br />
<br />
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* '''Dec-10 Th'''<br />
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* '''Dec-11 Fr'''<br />
<br />
2020 Week 51<br />
<br />
* '''Dec-14 Mo''' <br />
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* '''Dec-15 Tu'''<br />
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* '''Dec-16 We'''<br />
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* '''Dec-17 Th'''<br />
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* '''Dec-18 Fr'''<br />
<br />
2020 Week 52<br />
<br />
* '''Dec-21 Mo''' <br />
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* '''Dec-22 Tu'''<br />
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* '''Dec-23 We'''<br />
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* '''Dec-24 Th'''<br />
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* '''Dec-25 Fr'''<br />
<br />
2020 Week 53<br />
<br />
* '''Dec-28 Mo''' <br />
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* '''Dec-29 Tu'''<br />
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* '''Dec-30 We'''<br />
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* '''Dec-31 Th'''<br />
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* '''Jan-01 Fr'''</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206280
Template talk:Next oroboros ecosystem agenda
2020-08-20T06:11:13Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
* '''Aug-26 We''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Why measuring ROX is important? ''' <br />
''Rox'' is not necessarily equivalent to non-mitochondrial reduction of O<sub>2</sub>. <br><br />
- »'''[[Residual oxygen consumption]]'''« and »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''«<br />
|}<br />
:::: Suggestion 1 by Timi:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Why using the term [[residual oxygen consumption]] (''Rox'') is more accurate than non-mitochondrial respiration? ''' <br />
''Rox'' refers rather to ''mitochondrial non-ET-pathway'' than ''non-mitochondrial respiration'', because ''Rox'' can be also detected in purified mitochondria after ET-pathway inhibition, and it can be higher in [[Permeabilized cells| pce]] and [[living cells]] than in isolated mitochondria. <br><br />
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''«<br />
|}<br />
<br />
::::* I would change the question or keep the question but have a different answer, because I think that answer does not tell you why meausurement of ROX is important.<br />
::::* I moved the link to ROX to the question, because it might not be obvious for everyone what ROX means. Do you think we can have just one link to the BEC?<br />
::::* Should be define here what ROX means or the link is enough?<br />
<br />
:::: Suggestion 2 by Timi:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Why measuring [[residual oxygen consumption]] (''Rox'') is important? ''' <br />
''Rox'' refers to mitochondrial non-ET pathway related respiration rather than non-mitochondrial respiration due to oxidative side reactions remaining after ET-pathway inhibition. <br><br />
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''«<br />
|}<br />
::::* I am not sure that this is a real answer to this question. <br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''###-## Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Aug-25 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]«<br />
*Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. [[Liepinsh 2020 Physiol Rep|»Bioblast link«]]<br />
|}<br />
<br />
* '''Sep-01 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment'''<br><br />
:from the O2k-Network »[[US PA Philadelphia Kilbaugh T]]«, »[[US PA Philadelphia Jang DH]]« and »[[SE Lund Elmer E]]«<br />
*Janowska JI, Piel S, Saliba N, Kim CD, Jang DH, Karlsson M, Kilbaugh TJ, Ehinger JK (2020) Mitochondrial respiratory chain Complex I dysfunction induced by N-methyl carbamate ex vivo can be alleviated with a cell-permeable succinate prodrug carbamate toxicity and treatment. Toxicol In Vitro 65:104794. [[Janowska 2020 Toxicol In Vitro|»Bioblast link«]]<br />
|}<br />
<br />
::: '''Comment:''' [[User:Garcia Luiz|Garcia Luiz]] ([[User talk:Garcia Luiz|talk]]) 13:22, 18 August 2020 (CEST) - I am not sure if this one should be included. Despite the use of NV118, the terminology use is not in accordance to the preprint.<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed 2 mL chamber at air saturation without a biological sample? '''<br><br />
This may be due to [[biological contamination]] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [[O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber|Cleaning of the O2k-Chamber]]«<br><br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-Open_Support&diff=206223
O2k-Open Support
2020-08-18T09:31:44Z
<p>Antunes Diana: </p>
<hr />
<div>{{Template:OROBOROS support page name}}<br />
{{NextGen-O2k H2020-support}}<br />
[[File:O2k-support system.jpg|right|350px|Oroboros support system]]<br />
:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big> <br />
__TOC__<br />
<br />
== O2k-Open Support: technical help ==<br />
<br />
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed. Support regarding data analysis and/or interpretation is provided on an individual basis, for which consultancy fees apply. <br />
::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.<br />
::::::## '''[[MitoPedia: O2k-Open Support]]'''<br />
::::::## [[MitoPedia: O2k hardware]]<br />
::::::## [[MitoPedia: DatLab]]<br />
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments<br />
::::::# [[O2k-Manual]]: topic specific manuals with step by step help<br />
::::::# [[O2k-Videosupport]]<br />
::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].<br />
<br />
<br />
::: '''<big>O2k-Quality Control</big>'''<br />
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]<br />
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]]. <br />
<br />
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]]. <br />
::::# Clean O2k-chambers and contamination-free incubation medium are required. <br />
::::# Analysis of DatLab files recorded in instrumental QC tests is essential. <br />
::::# Biological experiments are not suitable for trouble shooting. <br />
<br />
<br />
=== 1. O2 sensor test ===<br />
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:<br />
::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']] <br />
<br />
=== 2. O2k-chamber test ===<br />
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail: <br />
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']] <br />
<br />
[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]<br />
== O2k-Open Support agreement ==<br />
<br />
:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.<br />
::::» [[Search for defective O2k components]]<br />
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support'''] <br />
::::» [[O2k-Open Support agreement]]<br />
::::» [[O2k repair]]<br />
<br />
<br />
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]<br />
== The O2k-Network ==<br />
<br />
:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology. <br />
<br />
::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]] <br />
<br />
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]] <br />
<br />
:::: '''»''' [[Oroboros Laboratories|Oroboros O2k-Laboratory]] - open for innovation and cooperation. <br />
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry <br />
<br />
[[Category:O2k-Open Support]]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206222
Template talk:Next oroboros ecosystem agenda
2020-08-18T09:18:43Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
* '''Aug-26 We''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Why measuring ROX is important? ''' <br />
''Rox'' is not necessarily equivalent to non-mitochondrial reduction of O<sub>2</sub>. <br><br />
- »'''[[Residual oxygen consumption]]'''« and »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''«<br />
|}<br />
:::: Suggestion 1 by Timi:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Why using the term [[residual oxygen consumption]] (''Rox'') is more accurate than non-mitochondrial respiration? ''' <br />
''Rox'' refers rather to ''mitochondrial non-ET-pathway'' than ''non-mitochondrial respiration'', because ''Rox'' can be also detected in purified mitochondria after ET-pathway inhibition, and it can be higher in [[Permeabilized cells| pce]] and [[living cells]] than in isolated mitochondria. <br><br />
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''«<br />
|}<br />
<br />
::::* I would change the question or keep the question but have a different answer, because I think that answer does not tell you why meausurement of ROX is important.<br />
::::* I moved the link to ROX to the question, because it might not be obvious for everyone what ROX means. Do you think we can have just one link to the BEC?<br />
::::* Should be define here what ROX means or the link is enough?<br />
<br />
:::: Suggestion 2 by Timi:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Why measuring [[residual oxygen consumption]] (''Rox'') is important? ''' <br />
''Rox'' refers to mitochondrial non-ET pathway related respiration rather than non-mitochondrial respiration due to oxidative side reactions remaining after ET-pathway inhibition. <br><br />
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]'''«<br />
|}<br />
::::* I am not sure that this is a real answer to this question. <br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''###-## Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange via [[adenine nucleotide translocase]] (ANT) using the fluorophore [[Magnesium Green]] (MgG)''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Aug-25 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]«<br />
*Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. [[Liepinsh 2020 Physiol Rep|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPediaAlert2020_Archive&diff=206193
MitoPediaAlert2020 Archive
2020-08-17T06:22:32Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
== Aug 2020 ==<br />
* '''Aug-20 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to with the new Q-Module being developed as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
----<br />
* '''Aug-13 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Photosynthesis efficiency is reduced by 25% in plants and algae due the metabolic process called photorespiration. <br><br />
- »'''[[Photorespiration]]'''«<br />
|}<br />
----<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|}<br />
<br />
<br />
== Previous MitoPedia Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
----<br />
* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
----<br />
<br />
* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
----<br />
* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
----<br />
* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
<br />
== Jun 2020 ==<br />
* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
<br />
* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
<br />
* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_Red_method Incompatible substrates]'''«<br />
|}<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
<br />
* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
<br />
<br />
=== May 2020 ===<br />
<br />
* '''May-28 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
<br />
* '''May-21 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
|As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
* '''May-14 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
<br />
* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''<br />
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br><br />
- Learn more about - »'''[[Malate-anaplerotic pathway control state|malate-anaplerotic pathway control state]]'''« <br><br />
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« <br><br />
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
=== Apr 2020 ===<br />
<br />
* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]]. <br><br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
<br><br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. <br><br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
<br><br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br><br />
- »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
<br><br />
* '''Apr-08 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. <br><br />
- »'''[[SUIT-010 |SUIT-010]]'''«<br />
|}<br />
<br><br />
* '''Apr-02 Th''' <br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' <br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-16 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. <br><br />
- »'''[[SUIT-003 |SUIT-003]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. <br><br />
- »'''[[Blebbistatin]]'''«<br />
|}<br />
<br><br />
* '''Mar-06 Fr'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br><br />
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? <br><br />
- »'''[[Rapamycin]]'''«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-26 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. <br><br />
- »'''[[Noncoupled respiration]]'''«<br />
|}<br />
<br><br />
* '''Feb-24 Mo'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| More research is needed on the impact of viral infection on mitochondrial function. <br><br />
- »'''[[Viruses and mitochondrial medicine]]'''«<br />
|}<br />
<br><br />
* '''Feb-19 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. <br><br />
- »'''[[Spermidine]]'''«<br />
|}<br />
<br><br />
* '''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Why do we fight gas bubbles? <br><br />
- Dive deep into »'''[[Oxygen solubility]]'''«<br />
|}<br />
<br><br />
* '''Feb-12 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| What do metformin, melatonin and other bioactive mitObesity compounds have in common? <br><br />
- »'''[[MitObesity drugs]]'''«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-16 Th'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Oxygen flux]]''' <br><br />
- which SI units should be used?<br />
|}<br />
<br><br />
<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Did you ever think that units for body mass could be an issue? <br><br />
- »'''[[Body mass]]'''«<br />
|}<br />
<br><br />
* '''Jan-08 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-PublicationAlert2020_Archive&diff=206192
O2k-PublicationAlert2020 Archive
2020-08-17T06:21:28Z
<p>Antunes Diana: </p>
<hr />
<div>{{Template:OROBOROS Slogan}}<br />
::::<big>» Listed in '''[[Oroboros Ecosystem Agenda 2020]]'''</big><br />
</big>__TOC__<br />
<br />
== Aug 2020 ==<br />
<br />
<br />
* '''Aug-18 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration in PBMCs from patients with major depression'''<br><br />
:from the O2k-Network »[[DE Ulm Karabatsiakis A]]« and »[[DE Ulm Radermacher P]]«<br />
*Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. [[Karabatsiakis 2014 Transl Psychiatry |»Bioblast link«]]<br />
|}<br />
----<br />
* '''Aug-14 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br><br />
: from the O2k-Network »[[NZ Auckland Hickey AJ]]« <br><br />
* Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Aug-11 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring'''<br><br />
:from the O2k-Network »[[DE Bremerhaven Mark FC]]«<br />
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |»Bioblast link«]]<br />
|}<br />
----<br />
* '''Aug-04 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br><br />
:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]« <br />
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]<br />
|}<br />
<br />
<br />
<br />
<br />
== Previous O2k-Publication Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-28 Tu''' - World Hepatitis Day<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Hepatitis C virus protein expression is related to changes in mitochondrial respiration'''<br><br />
:from the O2k-Network »[[IT Foggia Capitanio N]]« <br />
*Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60. [[Ripoli 2009 J Virol|»Bioblast link«]]<br />
|}<br />
----<br />
<br />
* '''Jul-21 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br><br />
:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]« <br />
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. [[Lantier 2020 Diabetes|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Jul-14 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br><br />
:from the O2k-Network »[[BR Ribeirao Preto Alberici LC]]« <br />
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-07 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br><br />
:from the O2k-Network »[[FR Lille Duez H]]« <br />
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Jul-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br><br />
: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br><br />
* Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]<br />
|}<br />
<br />
=== Jun 2020 ===<br />
* '''Jun-02 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br><br />
: from the O2k-Network »[[AT Innsbruck Gnaiger E]]« , »[[CA Rimouski Blier PU]]« and »[[CA Edmonton Lemieux H]]« <br />
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jun-12 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ10 in the platelets of CKD patients''' <br><br />
: from the O2k-Network » [[ SK Bratislava Sumbalova Z]] « <br><br />
* Gvozdjáková A, Sumbalová Z, Kucharská J, Komlósi M, Rausová Z, Vančová O, Számošová M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q10 and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|»Bioblast link«]]<br />
<br />
|}<br />
----<br />
* '''Jun-16 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br><br />
:from the O2k-Network »[[CA Montreal Hepple RT]]« <br />
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jun-23 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br><br />
:from the O2k-Network »[[IT Verona Calabria E]]« <br />
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jun-30 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]« <br />
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]<br />
|}<br />
=== May 2020 ===<br />
<br />
* '''May-29 Fr'''<br />
:{|<br />
<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br><br />
: from the O2k-Network »[[BR Sao Paulo Silber AM]]« <br />
*Alberto Luévano-Martínez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. [[Alberto Luevano-Martinez 2020 FEBS Lett|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
<br />
* '''May-26 Tu''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br><br />
: from the O2k-Network »[[ AT Innsbruck Gnaiger E]]« and »[[ AT Innsbruck Oroboros]]« <br><br />
* Schöpf B, Weissensteiner H, Schäfer G, Fazzini F, Charoentong P, Naschberger A, Rupp B, Fendt L, Bukur V, Giese I, Sorn P, Sant’Anna-Silva AC, Iglesias-Gonzalez J, Sahin U, Kronenberg F, Gnaiger E, Klocker H (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. [[Schoepf 2020 Nat Commun|»Bioblast link«]]<br />
<br />
|}<br />
<br />
----<br />
* '''May-19 Tu''' <br />
:{|<br />
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''«<br><br />
: from the O2k-Network »[[Charles Anne-Laure]]« »[[Geny Bernard]]«<br />
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|»Bioblast link«]]<br />
|}<br />
<br />
* '''May-17 Su''' World Hypertension Day <br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br><br />
: from the O2k-Network »[[CZ Prague Houstek J]]« and »[[CZ Prague Kalous M]]«<br />
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|»Bioblast link«]]<br />
|}<br />
<br />
* '''May-15 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br><br />
: from the O2k-Network » [[ FI Helsinki Jacobs HT]]« , »[[ DE Magdeburg Gellerich FN]]« , »[[FI Tampere Dufour E]]« , »[[DE Magdeburg Debska-Vielhaber G]]« and »[[DE Frankfurt Wittig I]]«<br><br />
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet M, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich Frank N, Schlueter KD, Jacobs HT(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]<br />
|}<br />
<br />
<br />
* '''May-12 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Mitochondria - a driving force in adipocyte differentiation''' <br><br />
: from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br><br />
* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''May-05 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse''' <br><br />
: from the O2k-Network »[[FI Helsinki Jacobs HT]]« »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«<br><br />
* Szibor M, Gainutdinov T, Fernandez-Vizarra E, Dufour E, Gizatullina Z, Debska-Vielhaber G, Heidler J, Wittig I, Viscomi C, Gellerich FN, Moore AL (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]<br />
|}<br />
<br />
=== Apr 2020 ===<br />
* '''Apr-28 Tu''' <br />
<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The mitochondrial gas pedal: Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake''' <br><br />
: from the O2k-Network » [[FI Helsinki Jacobs HT]] « » [[DE Magdeburg Gellerich FN]] « and »[[DE Magdeburg Debska-Vielhaber G]]«<br><br />
* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. [[Szibor 2020 J Biol Chem|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-20 Mo''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''The effect of respiration buffer composition on mitochondrial metabolism and function''' <br><br />
: from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«<br><br />
* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-15 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies''' <br><br />
: from the O2k-Network »[[UY Montevideo Radi R]]«<br><br />
* García-Roche M, Casal A, Carriquiry M, Radi R, Celia Quijano C, Cassina A (2018) Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies. Redox Biol 17:207-12. - [[Garcia-Roche_2018_Redox_Biol|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-06 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Physical activity has positive effects on mitochondria from patients with occupational burnout''' <br><br />
: from the O2k-Network »[[CH Basel Eckert A]]«<br><br />
* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''<br><br />
: from the O2k-Network »[[DE Nuthetal Klaus S]]«<br><br />
* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - [[Ost 2020 EMBO Rep |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-01 We''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria'''<br><br />
: from the O2k-Network »[[FR Angers Gueguen N]]« and »[[BE Leuven Spinazzi M]]«<br><br />
* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|»Bioblast link«]]<br />
|}<br />
<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-20 Fr'''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''High-resolution respirometry in human prostate cancer biopsies and mtDNA analyzed in samples recovered from the O2k-chamber'''<br><br />
: from the O2k-Network »[[AT Innsbruck Oroboros]]«<br><br />
* Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 2020-03-20 [Epub ahead of print]. - [[Schoepf_2020_Nat_Commun |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-12 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis'''<br><br />
: from the O2k-Network »[[UK London Duchen MR]]«<br><br />
* Warne J, Pryce G, Hill J, Shi X, Lennerås F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-10 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Muscular exercise for training adipose tissue mitochondria'''<br><br />
: from the O2k-Network »[[DK Copenhagen Larsen S]]«<br><br />
* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-09 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Protein-specific mitochondrial respiratory capacity is higher in women than men'''<br><br />
: from the O2k-Network »[[SE Stockholm Cardinale DA]]«<br><br />
* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-04 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets'''<br><br />
: from the O2k-Network »[[US FL Orlando Goodpaster BH]]«<br><br />
* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Rapamycin suppresses senescence in galactose versus glucose cell culture'''<br><br />
: from the O2k-Network »[[US PA Philadelphia Orynbayeva Z]]«<br><br />
* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-02 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction''' <br><br />
: from the O2k-Network »[[CN Chongqing Zhu Z]]«<br />
* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |»Bioblast link«]]<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-27 Th''' <br />
<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase''' <br><br />
: from the O2k-Network »[[CZ Prague Houstek J]]« <br><br />
* Pajuelo-Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Feb-25 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''An active lifestyle protects cardiac mitochondria''' <br><br />
: from the O2k-Network »[[KR Busan Han J]]« - »[[KR Incheon Kwak HB]]« <br><br />
* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis''' <br><br />
: from the O2k-Network »[[US MI Ann Arbor Beard DA]]« <br><br />
* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. - [[Tyrrell 2019 Circ Res |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Feb-05 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease''' <br><br />
: from the O2k-Network »[[US FL Boca Raton Khamoui AV]]« <br><br />
* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Feb-03 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' <br><br />
: from the O2k-Network »[[US AR Little Rock Boersheim E]]« <br><br />
* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<br />
*'''Jan-28 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration of blood cells and cardiovascular disease''' <br><br />
: from the O2k-Network »[[FR Strasbourg Zoll J]]« <br><br />
* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - [[Alfatni 2020 J Clin Med |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Jan-18 Sa'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations''' <br><br />
: from the O2k-Network »[[CZ Prague Krajcova A]]« <br><br />
* Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Jan-17 Fr''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-close.jpg|left|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]]<br />
|<big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. <br><br />
: [[Scandurra_2010_Adv_Exp_Med_Biol |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Jan-15 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers''' <br><br />
: from the O2k-Network »[[AU Melbourne Stepto NK]]« <br><br />
* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - [[Jacques 2020 FASEB J |»Bioblast link«]]<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-OpenSupportAlert2020_Archive&diff=206191
O2k-OpenSupportAlert2020 Archive
2020-08-17T06:20:26Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
<br />
<br />
== Aug 2020 ==<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed chamber without a biological sample? '''<br><br />
This may be due to [https://wiki.oroboros.at/index.php/Biological_contamination biological contamination] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [https://wiki.oroboros.at/index.php/O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber Cleaning of the O2k-Chamber]«<br><br />
|}<br />
----<br />
* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br><br />
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]«<br />
|}<br />
<br />
<br />
== Previous O2k-Open Support Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
<br />
<br />
== Jul 2020 ==<br />
<br />
* '''Jul-27 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''<br />
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br><br />
- » [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«<br />
|} <br />
----<br />
<br />
* '''Jul-20 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''How can you compare respirometric experiments using an internal normalization?'''<br><br />
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”. <br />
<br><br />
- »[[Flux control ratio]]«<br />
<br />
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
----<br />
* '''Jul-13 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br><br />
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. <br />
For more information, see:<br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP Copy from file]«<br />
|}<br />
----<br />
<br />
* '''Jul-06 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br><br />
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear. <br />
For more information, see:<br />
- »[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]« and »[[DatLab error messages]] «<br />
|}<br />
<br />
== Jun 2020 ==<br />
<br />
* '''Jun-01 Mo''' <br />
:{|<br />
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«<br />
|}<br />
----<br />
* '''Jun-15 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
<br />
----<br />
<br />
* '''Jun-22 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Did you know a stirrer test can save your experiments?'''<br><br />
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
* '''Jun-29 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br><br />
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br><br />
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«<br />
|}<br />
=== May 2020 ===<br />
<br />
* '''May-25 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br><br />
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br><br />
- »[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«<br />
|}<br />
<br />
* '''May-18 Mo''' <br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br><br />
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br><br />
- »[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«<br />
|}<br />
<br />
* '''May-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|''' Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.''' <br><br />
Learn more about steady states including performance and mark setting for data analysis. <br><br />
- »[[Steady state]]«<br />
|}<br />
<br><br />
* '''May-04 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you load the correct instrumental background correction according to the actual of experimental O<sub>2</sub> concentrations?'''<br><br />
Perform and apply the instrumental background test.<br><br />
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«<br />
|}<br />
=== Apr 2020 ===<br />
* '''Apr-27 Mo'''<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''<br><br />
The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub> concentrations.<br><br />
- »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«<br />
|}<br />
<br />
<br><br />
* '''Apr-20 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Improve]]<br />
|'''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''<br><br />
Unstable respiratory fluxes in OXPHOS might be due to substrates limitation.<br><br />
- »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«<br />
|}<br />
<br><br />
* '''Apr-14 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Why are the oxygen levels different between chambers prior to my experimental run?'''<br><br />
After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations.<br><br />
- »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«<br />
|}<br />
<br><br />
* '''Apr-07 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|70px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]]<br />
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''<br><br />
If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]].<br><br />
- »[[SUIT: Browse DL-Protocols and templates]]«<br />
|}<br />
<br><br />
* '''Apr-02 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|70px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]][[File:Blue-info.png|25px|Improve]]<br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?'''<br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-30 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:'''<br><br />
- »'''[[Enable DL-Protocol editing]]'''«<br />
|}<br />
<br><br />
* '''Mar-25 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4:<br><br />
- »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br />
|}<br />
<br><br />
* '''Mar-23 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''<br><br />
'''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br><br />
'''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''<br />
While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration.<br><br />
- »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«<br />
|}<br />
<br><br />
* '''Mar-04 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
| [[File:OroboPOS-Holder002.jpg|100px]]<br />
| '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''<br />
See how you can check that the two blue OroboPOS-Holders are intact.<br><br />
- »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-28 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Calibration parameters are transferred to experimental DatLab files'''<br />
Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.<br><br />
- »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-31 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''<br />
In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP).<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br><br />
* '''Jan-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''<br />
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample.<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you set mark ‘R1’ and complete the air calibration correctly?'''<br />
Your air calibration is not effective until you set mark ‘R1’ and click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window.<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br><br />
*'''Jan-24 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''<br />
Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed.<br><br />
- »[[O2k configuration]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
| '''Do you perform O2k-Chamber volume calibration?'''<br />
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]).<br><br />
- »[[O2k-Chamber MitoPedia]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?'''<br />
The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration.<br><br />
- »[[Oxygen solubility factor]]«<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Current_week_ecosystem_agenda&diff=206190
Template:Current week ecosystem agenda
2020-08-17T06:19:05Z
<p>Antunes Diana: </p>
<hr />
<div> 2020 Week 34<br />
<br />
* '''Aug-17 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Have you observed an 'O<sub>2</sub> slope neg.' > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed chamber without a biological sample? '''<br><br />
This may be due to [https://wiki.oroboros.at/index.php/Biological_contamination biological contamination] of the medium or the O2k-Chamber. <br><br />
For further details on how to clean the O2k-Chamber properly, see: <br><br />
- » [https://wiki.oroboros.at/index.php/O2k-Chamber_MitoPedia#Cleaning_the_O2k-Chamber Cleaning of the O2k-Chamber]«<br><br />
|}<br />
----<br />
* '''Aug-18 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration in PBMCs from patients with major depression'''<br><br />
:from the O2k-Network »[[DE Ulm Karabatsiakis A]]« and »[[DE Ulm Radermacher P]]«<br />
*Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. [[Karabatsiakis 2014 Transl Psychiatry |»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Aug-19 We'''<br />
<br />
----<br />
* '''Aug-20 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to with the new Q-Module being developed as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
----<br />
* '''Aug-21 Fr'''</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=206189
Oroboros Ecosystem Agenda 2020 Archive
2020-08-17T06:17:58Z
<p>Antunes Diana: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
[[File:Oroboros_EcosystemAgenda_2020.png|right|560px|Oroboros Ecosystem Agenda 2020]]<br />
{{Template:OROBOROS Slogan}}<br />
<br />
::::<big>» [[Oroboros Ecosystem Agenda 2020 |Current Oroboros Ecosystem Agenda 2020]]</big><br />
<br />
__TOC__<br />
2020 Week 33<br />
<br />
----<br />
* '''Aug-10 Mo''' <br />
<br />
----<br />
* '''Aug-11 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring'''<br><br />
:from the O2k-Network »[[DE Bremerhaven Mark FC]]«<br />
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Aug-12 We'''<br />
:{|<br />
| width="100" align="center" |[[File:MitoFit Preprint Arch pdf.png|100px|link=https://wiki.oroboros.at/images/4/4a/Gnaiger_MitoFit_Preprint_Arch_2020.4_doi_10.26214mitofit.200004.pdf |MitoFit pdf]]<br />
|'''Some terms in “Mitochondrial physiology” ''Bioenergetics Communications'' 2020.1 (States and rates) did not look familiar ― what is the elementary unit [x]?''' <br />
<br />
Fundamental answers, a critical discussion on the SI unit for ''count'', and the meaning of Euclid’s unit and Euclid’s number are presented in an unusual ‘'''canonical review'''’:<br />
<br />
* Gnaiger Erich (2020) Canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 [https://www.mitofit.org/images/4/4a/Gnaiger_MitoFit_Preprint_Arch_2020.4_doi_10.26214mitofit.200004.pdf doi:10.26124/mitofit:200004]. - [[Gnaiger_MitoFit_Preprint_Arch_2020.4 |'''»Bioblast link«''']]<br />
|}<br />
<br /><br />
<br />
:{|<br />
<br />
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]<br />
|Join the discussion on the MitoEAGLE Consortium Communication: “Mitochondrial physiology” ''Bioenergetics Communications'' 2020.1<br />
<br />
» [https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1#On_a_Comment_in_Nature_Metabolism '''On a Comment in ''Nature Metabolism'''''] «<br />
|}<br />
<br />
----<br />
* '''Aug-13 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Photosynthesis efficiency is reduced by 25 % in plants and algae due the metabolic process called photorespiration. <br><br />
- »'''[[Photorespiration]]'''«<br />
|}<br />
----<br />
* '''Aug-14 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br><br />
: from the O2k-Network »[[NZ Auckland Hickey AJ]]« <br><br />
* Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|»Bioblast link«]]<br />
|}<br />
2020 Week 32<br />
* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br><br />
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]«<br />
|}<br />
----<br />
* '''Aug-04 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br><br />
:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]« <br />
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Aug-05 We'''<br />
<br />
----<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|} <br />
----<br />
* '''Aug-07 Fr'''<br />
2020 Week 31<br />
<br />
* '''Jul-27 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''<br />
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br><br />
- »[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«<br />
|} <br />
<br />
----<br />
* '''Jul-28 Tu''' - World Hepatitis Day<br />
:{|<br />
<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Insights into Hepatitis C virus-linked mitochondrial dysfunction''' <br><br />
: from the O2k-Network »[[IT Foggia Capitanio N]]« <br><br />
* Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60. [[Ripoli 2009 J Virol|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-29 We''' <br />
<br />
----<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
----<br />
* '''Jul-31 Fr'''<br />
<br />
2020 Week 30<br />
<br />
* '''Jul-20 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''How can you compare respirometric experiments using an internal normalization?'''<br><br />
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”. <br />
<br><br />
- »[[Flux control ratio]]«<br />
<br />
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
----<br />
* '''Jul-21 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br><br />
:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]« <br />
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. [[Lantier 2020 Diabetes|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Jul-22 We''' <br />
<br />
<br />
----<br />
* '''Jul-23 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]<br />
| '''First [[O2k-Webinars|O2k-Webinar]] - O2k-Applications: H<sub>2</sub>O<sub>2</sub> production by [[Komlodi Timea|Dr. Tímea Komlódi]] ''' <br />
|}<br />
----<br />
* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
<br />
<br />
2020 Week 29<br />
<br />
* '''Jul-13 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br><br />
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. <br />
For more information, see:<br />
- »[[Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP|Copy from file]]«<br />
|}<br />
----<br />
* '''Jul-14 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br><br />
<br />
:from the O2k-Network »[[BR Ribeirao Preto Alberici LC]]« <br />
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|»Bioblast link«]]<br />
|}<br />
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* '''Jul-15 We''' <br />
<br />
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----<br />
* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
<br />
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* '''Jul-17 Fr'''<br />
----<br />
<br />
<br />
2020 Week 28 <br />
<br />
* '''Jul-06 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br><br />
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear.<br />
For more information, see:<br><br />
- »[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]« and »[[DatLab error messages]] «<br />
|}<br />
----<br />
* '''Jul-07 Tu''' <br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br><br />
:from the O2k-Network »[[FR Lille Duez H]]« <br />
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|»Bioblast link«]]<br />
|}<br />
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* '''Jul-08 We''' <br />
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----<br />
* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
----<br />
* '''Jul-10 Fr'''<br />
<br />
== Jun 2020 ==<br />
2020 Week 27<br />
<br />
* '''Jun-29 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br><br />
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br><br />
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«<br />
|}<br />
----<br />
* '''Jun-30 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]« <br />
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]<br />
|}<br />
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* '''Jul-01 We''' <br />
:{|<br />
| width="100" align="center" |[[Image:TRANSMIT Acronym.jpg|70px|link=https://wiki.oroboros.at/index.php/TRANSMIT#TRANSMIT_dissemination |TRANSMIT]]<br />
|'''Kick-off of the TRANSMIT crowdfunding campaign: NOW'''<br><br />
:Bring the world of scientific research closer to the world of patients by supporting four cancer patients’ associations.<br />
<br />
:- »[https://www.ideaginger.it/progetti/now.html https://www.ideaginger.it/progetti/now.html]«<br />
|}<br />
----<br />
* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
<br />
----<br />
* '''Jul-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br><br />
: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br><br />
* Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
<br />
2020 Week 26<br />
<br />
* '''Jun-22 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Did you know how a stirrer test can save your experiments?'''<br><br />
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
----<br />
* '''Jun-23 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br><br />
:from the O2k-Network »[[IT Verona Calabria E]]« <br />
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|»Bioblast link«]]<br />
|}<br />
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* '''Jun-24 We'''<br />
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* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
<br />
----<br />
* '''Jun-26 Fr'''<br />
<br />
{|<br />
| width="110" align="center" | <br />
<br />
[[Image:MitoGlobal.jpg|80px|left|MitoGlobal]]<br />
<br />
| Join and disseminate the [https://mitowomen.org/index.html '''“Women in Mitochondria”'''] initiative which aims to make the women working in the mitochondrial field more visible, contributing to accelerating equality and diversity. <br><br />
|}<br />
<br />
2020 Week 25<br />
<br />
* '''Jun-15 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
<br />
----<br />
* '''Jun-16 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br><br />
:from the O2k-Network »[[CA Montreal Hepple RT]]« <br />
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|»Bioblast link«]]<br />
|}<br />
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* '''Jun-17 We''' <br />
<br />
----<br />
* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
<br />
----<br />
* '''Jun-19 Fr'''<br />
<br />
2020 Week 24<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
----<br />
* '''Jun-09 Tu''' <br />
<br />
----<br />
* '''Jun-10 We'''<br />
----<br />
* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
<br />
----<br />
* '''Jun-15 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|''' Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
----<br />
* '''Jun-12 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ<sub>10</sub> in the platelets of CKD patients''' <br><br />
: from the O2k-Network » [[ SK Bratislava Sumbalova Z]] « <br><br />
* Gvozdjáková A, Sumbalová Z, Kucharská J, Komlósi M, Rausová Z, Vančová O, Számošová M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q<sub>10</sub> and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|»Bioblast link«]]<br />
<br />
|}<br />
<br />
<br />
2020 Week 23 <br />
<br />
* '''Jun-01 Mo''' <br />
:{|<br />
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«<br />
|}<br />
----<br />
* '''Jun-02 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br><br />
:from the O2k-Network »[[AT Innsbruck Gnaiger E]]« , »[[CA Rimouski Blier PU]]« and »[[CA Edmonton Lemieux H]]« <br />
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|»Bioblast link«]]<br />
|}<br />
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* '''Jun-03 We''' <br />
<br />
----<br />
* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Mitochondria show variable preferences for [[GM|glutamate & malate]] or [[PM|pyruvate & malate]] to support NADH-linked respiration, depending on species, tissues, and pathophysiological state.''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to compare respiratory capacities supported by these NADH-linked fuel substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
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* '''Jun-05 Fr'''<br />
<br />
<br />
---- <br />
== May 2020 ==<br />
2020 Week 22 <br />
<br />
* '''May-25 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br><br />
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br><br />
- »[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«<br />
|}<br />
----<br />
* '''May-26 Tu''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br><br />
: from the O2k-Network » [[ AT Innsbruck Gnaiger E]] « and » [[ AT Innsbruck Oroboros]] « <br><br />
* Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. [[Schoepf 2020 Nat Commun|»Bioblast link«]]<br />
<br />
|}<br />
----<br />
* '''May-27 We''' <br />
:{| <br />
| width="100" align="center" |[[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]<br />
|The decline of mitochondrial respiratory function is strongly associated with obesity. This may play a potential role in determining corona virus severity. Read more: https://wiki.oroboros.at/index.php/Viruses_and_mitochondrial_medicine<br />
<br />
To fight COVID-19 the world urgently needs effective and safe diagnostics, treatments and vaccines. The EU is organizing a Global Response pledging effort to finance life-saving solutions. Join the marathon and spread the word: https://europa.eu/global-response/#UnitedAgainstCoronavirus<br />
<br />
|}<br />
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* '''May-28 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
----<br />
* '''May-29 Fr'''<br />
<br />
:{|<br />
<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br><br />
:from the O2k-Network »[[BR Sao Paulo Silber AM]]« <br />
*Alberto Luévano-Martínez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. [[Alberto Luevano-Martinez 2020 FEBS Lett|»Bioblast link«]]<br />
|}<br />
<br />
2020 Week 21 <br />
<br />
* '''May-18 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br><br />
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br><br />
- »[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«<br />
|}<br />
<br />
----<br />
* '''May-19 Tu''' <br />
:{|<br />
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''«<br><br />
:from the O2k-Network »[[Charles Anne-Laure]]« »[[Geny Bernard]]«<br />
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|»Bioblast link«]]<br />
|}<br />
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* '''May-20 We''' <br />
:{|<br />
| width="100" align="center"|[[Image:SI-units.png|65px|link=https://wiki.oroboros.at/index.php/BEC2020.1_doi10.26124bec2020-0001.v1#SI_units|SI Units]]<br />
|'''SI units'''<br><br />
One year ago, on [https://www.euramet.org/publications-media-centre/news/?tx_news_pi1%5Bnews%5D=787&tx_news_pi1%5Baction%5D=detail&tx_news_pi1%5Bcontroller%5D=News World Metrology Day] (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of '''Mitochondrial physiology''' and with the launch of an innovative journal — '''[[Bioenergetics Communications]]'''.<br />
|}<br />
<br />
:{|<br />
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]<br />
|MitoEAGLE ''Consortium Communication'': '''Mitochondrial physiology''' ― Published online: 2020-05-20.<br />
Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1<br><br />
» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1<br />
|}<br />
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----<br />
<br />
* '''May-21 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
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* '''May-22 Fr'''<br />
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2020 Week 20<br />
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* '''May-11 Mo'''<br />
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:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.'''<br><br />
Learn more about steady states including performance and mark setting for data analysis <br><br />
- »[[Steady state]]«<br />
|}<br />
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* '''May-12 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Mitochondria - a driving force in adipocyte differentiation''' <br><br />
:from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br><br />
* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]<br />
|}<br />
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* '''May-13 We'''<br />
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* '''May-14 Th'''<br />
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:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
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* '''May-15 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br><br />
: from the O2k-Network »[[ FI Helsinki Jacobs HT]]« , »[[ DE Magdeburg Gellerich FN]]« , »[[FI Tampere Dufour E]]« , »[[DE Magdeburg Debska-Vielhaber G]]« and »[[DE Frankfurt Wittig I]]«<br><br />
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet Ma, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT (2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]<br />
|}<br />
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* '''May-17 Su''' World Hypertension Day <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br><br />
:from the O2k-Network »[[CZ Prague Houstek J]]« and »[[CZ Prague Kalous M]]«<br />
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|»Bioblast link«]]<br />
|}<br />
2020 Week 19<br />
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* '''May-04 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br><br />
Perform and apply the instrumental background test:<br><br />
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«<br />
|}<br />
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* '''May-05 Tu''' <br />
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''<br />
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]« »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«<br />
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]<br />
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* '''May-06 We''' <br />
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* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: »'''[[Malate-anaplerotic pathway control state]]'''«<br />
<br />
- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br />
<br />
- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
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* '''May-08 Fr'''<br />
<br />
== Apr 2020 ==<br />
<br />
2020 Week 18<br />
<br />
* '''Apr-27 Mo''' <br />
:::: [[Image:O2k-Support.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''<br />
::::::: The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub> concentrations. - »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«<br />
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* '''Apr-28 Tu''' <br />
::::[[Image:O2k-Publications.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake'''<br />
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«, »[[DE Magdeburg Gellerich FN]]« and »[[DE Magdeburg Debska-Vielhaber G]]« <br />
::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - [[Szibor 2020 J Biol Chem|»Bioblast link«]]<br />
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* '''Apr-29 We''' <br />
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* '''Apr-30 Th'''<br />
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* '''May-01 Fr'''<br />
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2020 Week 17<br />
* '''Apr-20 Mo''' <br />
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:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''<br />
<br />
::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«<br />
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]<br />
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''<br />
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«<br />
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* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.<br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
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* '''Apr-22 We''' <br />
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* '''Apr-23 Th''' <br />
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* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]].<br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
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2020 Week 16<br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''<br />
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of [[mitochondrial preparations]] - »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
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* '''Apr-14 Tu''' <br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''<br />
::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«<br />
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* '''Apr-15 We''' <br />
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* '''Apr-16 Th'''<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease'''<br />
<br />
::::::: - from the O2k-Network »[[DE Frankfurt Eckert GP]]« »[[CH Basel Eckert A]]«<br />
::::::* Leuner K, Schulz K, Schütt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, Müller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204. - [[Leuner 2012 Mol Neurobiol|»Bioblast link«]] <br />
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* '''Apr-17 Fr'''<br />
2020 Week 15<br />
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* '''Apr-06 Mo''' <br />
<br />
<big><big>{{Template:Humankind COVID-19}}</big></big><br />
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<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Physical activity has positive effects on mitochondria from patients with occupational burnout''' <br />
::::::: - from the O2k-Network »[[CH Basel Eckert A]]«<br />
::::::* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|»Bioblast link«]]<br />
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* '''Apr-07 Tu''' <br />
:::: [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[File:Blue-info.png|40px|Info: improve]] '''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''''<br />
<br />
::::::: If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. - »[[SUIT: Browse DL-Protocols and templates]]«<br />
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* '''Apr-08 We''' <br />
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''<br />
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - »'''[[SUIT-010 |SUIT-010]]'''«<br />
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* '''Apr-09 Th'''<br />
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* '''Apr-10 Fr'''<br />
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2020 Week 14<br />
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* '''Mar-30 Mo'''<br />
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:''' »'''[[Enable DL-Protocol editing]]'''«<br />
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* '''Mar-31 Tu''' <br />
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* '''Apr-01 We''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria''' <br />
::::::: - from the O2k-Network »[[FR Angers Gueguen N]]« and »[[BE Leuven Spinazzi M]]«<br />
::::::* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|»Bioblast link«]]<br />
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[[File:Tony hickey garage.jpg|200px|thumb|Non-stoppable: Tony Hickey in home-office mode on high-resolution respirometry]]<br />
* '''Apr-02 Th''' <br />
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?''' Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
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::::[[Image:O2k-Network.png|70px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] Creative solutions coming from the O2k-Network labs - » [[NZ Auckland Hickey AJ]] <br />
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* '''Apr-03 Fr'''<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''<br />
<br />
::::::: - from the O2k-Network »[[DE Nuthetal Klaus S]]«<br />
::::::* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - [[Ost 2020 EMBO Rep |»Bioblast link«]] <br />
<br /><br />
== Mar 2020 ==<br />
2020 Week 13<br />
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* '''Mar-23 Mo''' <br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''<br />
::::::: '''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br><br />
::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''«<br />
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* '''Mar-24 Tu'''<br />
:::: [[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Tuberculosis Day: '''Mitochondria as target to ''Mycobacterium tuberculosis'' infection''' <br />
::::::: - from the O2k-Network »[[US NC Durham Li PA]]«<br />
::::::* Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - [[Lu 2018 ACS Infect Dis |»Bioblast link«]]<br />
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* '''Mar-25 We''' <br />
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] Preprint: '''An urgent call for research on COVID-19 and public vitamin D awareness'''<br />
::::::* Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. MitoFit Preprint Arch [[doi:10.26124/mitofit:200001]].<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Research findings made available via preprint servers before journal publication''' - [https://wellcome.ac.uk/press-release/sharing-research-data-and-findings-relevant-novel-coronavirus-covid-19-outbreak?sap-outbound-id=CF563AB7AA7FEDFB8731215C98A59C11790C0456&utm_source=hybris-campaign&utm_medium=email&utm_campaign=101_PBN2619_0000020211_GLS_101_aut_202003_Coronavirus_Forschung&utm_content=EN_internal_44988_20200325&mkt-key=005056A5C6311EE9999982134B93151D Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak]<br />
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]]'''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4: »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br />
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* '''Mar-26 Th'''<br />
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* '''Mar-27 Fr'''<br />
<br />
2020 Week 12 <br />
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* '''Mar-16 Mo''' <br />
::::: [[Image:Logo_OROBOROS_INSTRUMENTS.jpg|left|60px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]] '''Oroboros operates from now on in home office mode - we stay connected.'''<br />
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:::: [[Image:SUIT icon.jpg|80px|left|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br />
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »'''[[SUIT-003 |SUIT-003]]'''«<br />
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* '''Mar-17 Tu'''<br />
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::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ''' Zika virus infection leads to mitochondrial dysfunction''' <br />
::::::: - from the O2k-Network »[[BR Rio de Janeiro Galina A]]«<br />
::::::* Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - [[Ledur 2020 Sci Rep |»Bioblast link«]]<br />
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* '''Mar-18 We''' <br />
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:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Exercise protects against the effects of aging on mitochondrial function'''<br />
<br />
::::::: - from the O2k-Network »[[KR Busan Han J]]« »[[KR Incheon Kwak HB]]«<br />
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]] <br />
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* '''Mar-19 Th''' <br />
:::: [[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis '''Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home'''.<br />
::::::: - » For the download link and password, please email [mailto:instruments@oroboros.at instruments@oroboros.at]<br />
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* '''Mar-20 Fr'''<br />
::::* '''Abstract submission deadline''' - »[[SHVM 2020 Halifax CA]]«<br />
<br />
2020 Week 11<br />
* '''Mar-08 Su''' - '''[https://www.internationalwomensday.com/ International Women's Day]''' <br />
<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Addressing the underrepresentation of women |»Bioblast link«]]<br />
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* '''Mar-09 Mo''' <br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] »<big>'''[[MitoEAGLE WG2 Innsbruck AT]]'''</big>« (Mar 09-13)<br />
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<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Protein-specific mitochondrial respiratory capacity is higher in women than men''' <br />
::::::: - from the O2k-Network »[[SE Stockholm Cardinale DA]]«<br />
::::::* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |»Bioblast link«]]<br />
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* '''Mar-10 Tu'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Muscular exercise for training adipose tissue mitochondria''' <br />
::::::: - from the O2k-Network »[[DK Copenhagen Larsen S]]«<br />
::::::* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |»Bioblast link«]]<br />
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* '''Mar-11 We'''<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. - »'''[[Blebbistatin]]'''«<br />
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<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Let's talk COVID-19 - a new culture of communication in science.''' - [https://www.sciencemag.org/news/2020/02/completely-new-culture-doing-research-coronavirus-outbreak-changes-how-scientists Kay Kupferschmidt in Science]<br />
<br /><br />
<br />
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while operating the O2k with biological sample?'''<br />
::::::: - It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before [[uncoupler titrations]]. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«<br />
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* '''Mar-12 Th'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis''' <br />
::::::: - from the O2k-Network »[[UK London Duchen MR]]«<br />
::::::* Warne J, Pryce G, Hill J, Shi X, Lennerås F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |»Bioblast link«]] <br />
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* '''Mar-13 Fr'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Men and women with Type 1 diabetes and skeletal muscle mitochondria''' <br />
::::::: - from the O2k-Network »[[CA Toronto Perry CG]]«<br />
::::::* Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - [[Monaco 2020 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
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<br />
2020 Week 10<br />
<br />
* '''Mar-02 Mo''' <br />
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] An amazing number of new [[O2k-Publications: Topics |O2k-Publications]] from China. - »'''[[O2k-Network Labs China]]'''«<br />
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<br /><br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction''' <br />
::::::: - from the O2k-Network »[[CN Chongqing Zhu Z]]«<br />
::::::* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |»Bioblast link«]] <br />
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* '''Mar-03 Tu''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Rapamycin suppresses senescence in galactose versus glucose cell culture''' <br />
::::::: - from the O2k-Network »[[US PA Philadelphia Orynbayeva Z]]«<br />
::::::* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |»Bioblast link«]]<br />
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:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? - »'''[[Rapamycin]]'''«<br />
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<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Advancing Open Science practices: stakeholder perspectives on incentives and disincentives.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Advancing Open Science practices |»Bioblast link«]] - The concept of [[Gnaiger 2019 MitoFit Preprint Arch Editorial |Preprints]] needs full attention in Open Science.<br />
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* '''Mar-04 We'''<br />
[[File:Platelet.jpg|right|200px]]<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets''' <br />
::::::: - from the O2k-Network »[[US FL Orlando Goodpaster BH]]«<br />
::::::* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |»Bioblast link«]] <br />
<br />
[[File:Softdrink.jpg|right|100px]]<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Sugar and fat - initial mitochondrial aging in the brain'''<br />
::::::: - from the O2k-Network »[[IT Naples Iossa S]]«<br />
::::::* Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - [[Crescenzo 2019 Mol Neurobiol |»Bioblast link«]] <br />
[[File:OroboPOS-Holder002.jpg|right|100px]]<br />
<br /><br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''<br />
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«<br />
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* '''Mar-05 Th'''<br />
::::* '''Abstract submission opens''' - »[[Europhysiology 2020 Berlin DE]]«<br />
<br />
<br />
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] With a '''preprint''' you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - »'''[[Preprint]]'''«<br />
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* '''Mar-06 Fr'''<br />
:::: [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''Explore metabolic profiles and specific modultations of mitochondrial pathways''' in health and disease with SUIT reference protocols. - »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
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<br />
== Feb 2020 ==<br />
<br />
2020 Week 9<br />
<br />
* '''Feb-23 Su'''<br />
:::::::» '''[[2020 PaduaMuscleDays Padova IT]]''' - ''postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy''<br />
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* '''Feb-24 Mo''' <br />
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] More research is needed on the impact of viral infection on mitochondrial function. - »'''[[Viruses and mitochondrial medicine]]'''«<br />
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* '''Feb-25 Tu'''<br />
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] High quality mitochondrial research is increasing rapidly in South Korea. - »'''[[O2k-Network Labs Republic of Korea]]'''«<br />
<br /><br />
<br /><br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''An active lifestyle protects cardiac mitochondria''' <br />
::::::: - from the O2k-Network: [[KR Busan Han J]], [[KR Incheon Kwak HB]]<br />
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]] <br />
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[[File:2020-02-26 MitoEAGLE WG4 Innsbruck AT.jpg|right|400px|thumb|MitoEAGLE WG4 retreat; left to right: [[Garcia-Souza Luiz F |Luiz]], [[Gnaiger Erich |Erich]], [[Aasander Frostner Eleonor |Eleonor]], [[Michalak Slawomir |Slawomir]]]]<br />
* '''Feb-26 We''' <br />
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. - »'''[[Noncoupled respiration]]'''«<br />
<br /><br />
<br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] '''[[MitoEAGLE WG4 Innsbruck AT]]''' (Feb 26-28)<br />
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* '''Feb-27 Th'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase''' <br />
::::::: - from the O2k-Network: [[CZ Prague Houstek J]]<br />
::::::* Pajuelo-Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |»Bioblast link«]] <br />
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* '''Feb-28 Fr'''<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''<br />
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«<br />
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<br />
2020 Week 8<br />
<br />
* '''Feb-17 Mo''' <br />
:::: [[File:EBEClogo2020.jpg|80px|link=https://www.mitoglobal.org/index.php/EBEC2020_Marseille_FR]] '''Registration is open: [[EBEC2020_Marseille_FR |European Bioenergetics Conference 2020]]''', Marseille, France, 2020<br />
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* '''Feb-18 Tu''' <br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? » Dive deep into '''[[Oxygen solubility]]'''<br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis''' <br />
::::::: - from the O2k-Network »[[US MI Ann Arbor Beard DA]]«<br />
::::::* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. - [[Tyrrell 2019 Circ Res |»Bioblast link«]]<br />
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* '''Feb-19 We''' <br />
:::: [[Image:MitoFit Preprint Arch.png|80px|link=MitoFit Preprint Arch]] - »'''[[MitoFit Preprint Arch]]'''« was initiated one year ago. We invite submissions of mitochondrial preprints.<br />
<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »'''[[Spermidine]]'''«<br />
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* '''Feb-20 Th'''<br />
:::: [[Image:Logo MitoFit s.jpg|40px|link=MitoFit Open Seminars]] - '''[[MitoFit Open Seminars|MitoFit Open Seminar]]''': [[Stefan_E|Eduard Stefan]]: “Decoding and Perturbation of ONCO-Kinase functions” and [[Torres-Quesada_O|Omar Torres Quesada]]: “'''[[MitoKIN]]''' - Systematic barcoding of kinase-controlled mitochondrial function”<br />
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<br />
2020 Week 7<br />
<br />
*'''Feb-10 Mo''' <br />
::::* '''Early Bird Registration Deadline''' for [[ESSR 2020 Innsbruck AT]] <br />
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*'''Feb-12 We''' <br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »'''[[MitObesity drugs]]'''«<br />
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<br />
:::: [[Image:O2k-Feedback.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] ''I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner.'' - '''[[Gayathri Narayanappa]]''' participaing in [[MiPNet25.03 IOC144 Innsbruck AT]]<br />
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*'''Feb-13 Th'''<br />
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2020 Week 6<br />
<br />
*'''Feb-03 Mo''' <br />
:::[[File:FFG-logo.png|150px|FFG]] '''<big>»Official start of the FFG funded project »[[MitoKIN|'''MitoKIN''']]«</big>''' <br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' <br />
::::::: - from the O2k-Network »[[US AR Little Rock Boersheim E]]« <br />
::::::* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
<br />
::::* Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, [https://wiki.oroboros.at/index.php/Tremblay_N Dr Nelly Tremblay] is using for the first time the [[SUIT-022]] protocol to study alternative oxidases in the marine model organism ''Phaeodactylum tricornutum''.<br />
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*'''Feb-04 Tu''' <br />
:::: [[Image:NextGenO2k logo.jpg|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.10 IOC and Distributor engagement Tokyo JP |'''MiPNet25.10 IOC and Distributor engagement Tokyo JP''']] (Feb 4-7)<br />
:::: [[File:O2k-brief.png|50px|link=https://wiki.oroboros.at/images/7/7b/Cronin_2018_Nature_O2k-brief.pdf|O2k-brief]] '''Cronin''' (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - '''Lymphocytes''', '''Cancer''' - [http://wiki.oroboros.at/index.php/O2k-brief O2k-brief repository]<br />
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*'''Feb-05 We'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease'''<br />
::::::: - from the O2k-Network »[[US FL Boca Raton Khamoui AV]]«<br />
::::::* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |»Bioblast link«]]<br />
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*'''Feb-06 Th'''<br />
:::: '''MitoFit Open Seminar'''<br />
:::::* 16:00-16:30 [[Beleza J|Jorge Beleza]]: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. [[Media:MitoFitOpenSeminar_JorgeBeleza_20200206.pdf|»Abstract«]]<br />
:::::* 16:30-17:00 [[Pereira R|Rita Pereira]]: The Handball4Health Project : A community-based intervention programme<br />
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<br />
== Jan 2020 ==<br />
<br />
2020 Week 5<br />
<br />
*'''Jan-27 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.06 IOC145 Innsbruck AT |<big>'''IOC145 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 27-29)<br />
<br />
:::: DSL-Seminar, 16:00<br />
:::::: [[Gnaiger E]]: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.<br />
:::::: Univ.-Klinik für Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room<br />
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*'''Jan-28 Tu'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration of blood cells and cardiovascular disease''' <br />
::::::: - from the O2k-Network »[[FR Strasbourg Zoll J]]« <br />
::::::* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - [[Alfatni 2020 J Clin Med |»Bioblast link«]]<br />
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*'''Jan-30 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Karabatsiakis_A|Alexander Karabatsiakis]]: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''<br />
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »[[Oxygen calibration - DatLab]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?'''<br />
<br />
::::::: Your air calibration is not effective until you set mark ‘R1’ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - »[[Oxygen calibration - DatLab]]« <br />
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<br />
::::* '''Open Innovation''' in the NextGen-O2k software development DatLab 8. Here is a summary of [[MitoPedia: DatLab |MitoPedia]] pages including [[:Category:DatLab 8 perspectives |'''DatLab 8 perspectives''']]<br />
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*'''Jan-31 Fr'''<br />
::::* Deadline for [[APS2020_Chicago_US|APS2020]] Abstract submission for poster presentation.'''<br />
::::* [[2020 PaduaMuscleDays Padova IT |Call for EJTM Communications]] - deadline for manuscript submissions<br />
::::* Registration deadline for [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''<br />
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »[[Oxygen calibration - DatLab]]«<br />
<br />
2020 Week 4<br />
<br />
*'''Jan-20 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.03 IOC144 Innsbruck AT |<big>'''IOC144 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 20-22)<br />
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*'''Jan-21 Tu'''<br />
::::* Mr Hai Wu (CEO of [[CN Beijing Huawei |Huawei]], Oroboros distributor in China) and Dr. Rupert Ecker (CEO of [https://www.tissuegnostics.com/en/TissueGnostics Vienna TissueGnostics]) join the O2k-Coaching Days for extending collaborative projects.<br />
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*'''Jan-23 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Gnaiger E|Erich Gnaiger]]: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.<br />
<br />
<br />
----<br />
*'''Jan-24 Fr'''<br />
::::* Further circulars to invited speakers for submission of tentative titles for oral and poster presentations - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''<br />
::::::: Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - »[[O2k configuration]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''<br />
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?<br />
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]«<br />
<br />
2020 Week 3<br />
<br />
*'''Jan-14 Tu''' <br />
::::* Circular ITC Grants for [[2020 PaduaMuscleDays Padova IT]], [[6th EU-Cardioprotection Meeting 2020 Riga LV]] and [[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]] - [https://wiki.oroboros.at/images/a/af/ITC_Newsletter.pdf pdf]<br />
<br />
<br />
----<br />
*'''Jan-15 We''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers''' <br />
::::::: - from the O2k-Network »[[AU Melbourne Stepto NK]]« <br />
::::::* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - [[Jacques 2020 FASEB J |»Bioblast link«]]<br />
<br />
<br />
----<br />
*'''Jan-16 Th'''<br />
::::[[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Oxygen flux]]''' - which SI units should be used?<br />
<br />
::::[[File:MitoPedia.jpg|60px|MitoPedia]] Did you ever think that units for body mass could be an issue? - »'''[[Body mass]]'''«<br />
<br />
<br />
----<br />
*'''Jan-17 Fr''' <br />
::::[[Image:O2k-close.jpg|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]] <big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. - [[Scandurra_2010_Adv_Exp_Med_Biol |»Bioblast link«]]<br />
<br />
:::: [[Image:TRANSMIT Acronym.jpg|60px|TRANSMIT]] <big>'''[[TRANSMIT Symposium 2020 Brussels BE]]'''</big> (Jan 17-18)<br />
<br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations''' <br />
::::::: - from the O2k-Network »[[CZ Prague Krajcova A]]« <br />
::::::* Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |»Bioblast link«]]<br />
<br />
2020 Week 2<br />
<br />
*'''Jan-07 Tu''' The Oroboros Office opens in the New Year 2020<br />
[[File:EBEClogo2022.jpg|right|300px|right|link=EBEC22 Innsbruck AT]]<br />
::::* From the '''Austrian Bioenergetics Group''' to '''Mitochondrial Physiology and Bioenergetics DACH''' (D: Germany; A: Austria; CH: Switzerland) <br />
:::::: [[EBEC22 Innsbruck AT |EBEC22 - the 22nd European Bioenergetics Conference]] will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the ''EBEC22 National Scientific Committee''. This provides an excellent opportunity to establish an ''Austrian Bioenergetics Group'' towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming [[1st Myocardial Function Symposium 2020 Graz AT]], and further at the [[MitoEAGLE Summit Obergurgl 2020]]. Please, contact the organizer of EBEC22 - erich.gnaiger@oroboros.at<br />
<br />
<br />
----<br />
* '''Jan-08 We'''<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
<br />
:::: [[Image:Quality.png|left|60px|link=http://wiki.oroboros.at/index.php/MitoPedia:_Oroboros_QM |Quality]] <br />
<br />
<br />
----<br />
* '''Jan-09 Th'''<br />
[[File:MITOEAGLE-representation.jpg|right|200px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]]<br />
::::* Planning the [[MitoEAGLE Summit Obergurgl 2020]]<br />
:::::: Members of the [[Management Committee MitoEAGLE]] and [[MitoEAGLE Working Groups |Working Group Leaders]]: please submit your suggestions for [[MitoEAGLE Summit Obergurgl 2020]]:<br />
::::::# Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output<br />
::::::# Suggested scientists (titles) for presentations, according to the MoU<br />
<br />
<br />
----<br />
* '''Jan-10 Fr''' <br />
::::» <big>'''[[1st Myocardial Function Symposium 2020 Graz AT]]'''</big> (Jan 10-11)<br />
::::* 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)<br />
<br />
<br />
----<br />
* '''Jan-12 So'''<br />
::::* One month ago<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:: '''Cluster Awards 2019'''<br />
<br />
<hr><br />
<br />
{|<br />
|[[File:Cluster Awards 2019.jpg|right|500px|Cluster Awards 2019]]<br />
On December 12<sup>th</sup>, the '''Cluster Awards 2019''' were presented for the best Tyrolean innovations of the year. We are thrilled to be the recipient in the '''Life Sciences Cluster''' for our EU Horizon 2020-funded '''[[NextGen-O2k |NextGen-O2k project]]'''.<br />
<br />
'''Life Sciences'''<br />
::: Oroboros Instruments GmbH, Innsbruck: "NextGen-O2k"<br />
<br />
'''Renewable Energies'''<br />
::: Heliotherm Wärmepumpentechnik GmbH, Langkampfen: "Invisible Heatpump"<br />
<br />
'''Forest and wood'''<br />
::: Holzbau Unterrainer GmbH, Ainet: "Radius wood - production of curved cross laminated timber elements"<br />
<br />
'''Information Technologies'''<br />
::: Lo.La Peak Solutions GmbH, Trins: "LO.LA Safety - Digital Safety Management in Ski Resorts"<br />
<br />
'''Mechatronics''': ALPEX Technologies GmbH, Mils: "EVEREST 4.0"<br />
<br />
'''Wellness''': Organoid Technologies GmbH, Flow: "natural sauna surfaces made of hay"<br />
<br />
» More details (in German): '''[https://www.standort-tirol.at/page.cfm?vpath=newsroom/news&genericpageid=26328 Standortagentur Tirol]'''.<br />
<div style="text-align: right; direction: ltr; margin-left: 1em;">Photo © Standortagentur Tirol</div><br />
|}<br />
|}<br />
<br />
2020 Week 1 '''The Oroboros team enjoys ''holidays'' from 2019 Dec 24 to 2020 Jan 06'''<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: #white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:'''[[Oroboros picture of the month]]'''<br />
<br />
<hr><br />
{|<br />
| <br />
[[File:Oroboros Winter.jpg|250px|Oroboros of the month]]<br />
| ''' Season's Greetings'''<br />
<br />
: Our office is closed until 2020-01-06. <br />
<br />
: We wish you a Happy New Year 2020! <br />
<br />
: Your Oroboros team<br />
::::::* the [[Oroboros_contact#Oroboros_Customer_Relations_Ninjas |Oroboros Customer Relations Ninjas]]<br />
::::::* the [[Oroboros_contact#Oroboros_Sales_Athletes |Oroboros Sales Athletes]]<br />
::::::* the [[Oroboros_contact#Oroboros_Bioenergetics_Adventurers |Oroboros Bioenergetics Adventurers]]<br />
::::::* the [[Oroboros_contact#Oroboros_Quality_Management_Angels |Oroboros Quality Management Angels]]<br />
::::::* the [[Oroboros_contact#Oroboros_Leadership_Group |Oroboros Leadership Group]]<br />
<br />
|}<br />
|}<br />
<br />
<br />
----<br />
*'''Jan-04 Sa'''<br />
:::: [[Image:O2k-News icon.jpg|60px|link=https://wiki.oroboros.at/index.php/Oroboros_News | News]] The [[Oroboros Ecosystem Agenda 2020]] is launched as a signature of the [[Oroboros Ecosystem]].<br />
<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] [https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(19)33170-8/fulltext?dgcid=raven_jbs_etoc_email 2020: a critical year for women, gender equity, and health - a Lancet Editorial] <br />
<br />
::::* One month ago<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:: '''NextGen-O2k SME2 coaching Orily Pratt part 2'''<br />
<br />
<hr><br />
<br />
{|<br />
|[[File:2019-12-04 Coaching with Orily Pratt.jpg|left|250px|MitoFit Guest Seminar]] On '''December 03/04''' the second part of the '''SME phase 2 Coaching for the [[NextGen-O2k]] project''' with '''Orily Pratt''' (Tel Aviv, IL) took place in Innsbruck. <br />
<br/><br />
<br/><br />
<br />
:» Wednesday, 4th of December; Oroboros Instruments, Schoepfstrasse 18, MiPArt, Innsbruck, Austria.<br />
<br />
|}<br />
|}<br />
<br />
<br />
== Stay alert ==<br />
{{Oroboros Ecosystem Agenda list}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=206117
Template talk:Next oroboros ecosystem agenda
2020-08-12T14:59:55Z
<p>Antunes Diana: /* O2k-Open Support alert */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Aug-25 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange by [[ANT]] using [[MgG]] with the O2k''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Aug-18 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration in PBMCs of major depression patients'''<br><br />
:from the O2k-Network »[[DE Ulm Karabatsiakis A]]« and »[[DE Ulm Radermacher P]]«<br />
*Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. [[Karabatsiakis 2014 Transl Psychiatry |»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
<br />
* '''Aug-24 Mo'''<br />
::Suggestion 1:<br />
{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Can NADH be directly used as substrate in respirometry? '''<br><br />
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for mitochondrial respiration since NADH is not permeable through the [[mitochondrial inner membrane]] (mtIM). The mtIM should be intact for respirometry experiments. <br><br />
- [[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide ]]<br />
|}<br />
<br />
::Suggestion 2:<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Warning: loss of data]]<br />
|'''Can NADH be directly used as substrate in respirometry? '''<br><br />
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for mitochondrial respiration since NADH is not permeable through the [[mitochondrial inner membrane]] (mtIM). The mtIM should be intact for respirometry experiments. <br><br />
- [[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide ]]<br />
|}<br />
<br />
::Suggestion 3:<br />
::1)high-resolution respirometry instead of respirometry;<br />
::2)added link for 'mitochondrial respiration';<br />
::3) HRR instead of respirometry;<br />
::4) would it make sense to add the link for substrates and metabolites where the customer can find other substrates to be used in HRR?<br />
::5)'»' between links. <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Warning: loss of data]]<br />
|'''Can NADH be directly used as substrate in high-resolution respirometry (HRR)? '''<br><br />
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for [[Mitochondrial_respiration |mitochondrial respiration]] since NADH is not permeable through the [[mitochondrial inner membrane]] (mtIM). The mtIM should be intact for HRR experiments. <br><br />
- » [[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide ]]» and »[[MitoPedia:_Substrates_and_metabolites|Substrates and metabolites]]» <br />
|}<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-Chambers&diff=206086
O2k-Chambers
2020-08-12T10:47:52Z
<p>Antunes Diana: </p>
<hr />
<div>{{MitoPedia<br />
|description='''O2k-Chamber''': 16 mm inner diameter, Duran glass polished, with standard operation volume (''V'') of 2 mL (2 cm<sup>3</sup>). The [[O2k-sV-Module]] has a small chamber volume of 0.5 mL.<br />
|info=[[O2k-Chamber]]<br />
}}<br />
__TOC__<br />
<br />
{{Template:Technical support integrated}}<br />
<br />
<br />
== O2k-Chamber: paradigm shift in HRR ==<br />
[[File:Glass chamber.jpg|right|thumb|300px|The glass chamber and PVDF stirrer of the O2k.]]<br />
::::* The glass chamber and PVDF stirrer of the O2k.<br />
::::* O2k: Paradigm shift and features of high-resolution respirometry. MiPNet09.01. Including sole source information. Bioblast Link<br />
<br />
<br />
:::: Small experimental chamber volumes of 20-50 µL generate problems rather than providing resolution with small amounts of tissue samples, low numbers of cultured cells or few isolated mitochondria. The surface to volume ratio increases with decreasing chamber volume, thus various boundary effects entail larger errors at smaller volume, in particular oxygen diffusion. While the rate of oxygen depletion per unit amount of sample increases linearly with decreasing chamber volume, side effects may increase to a larger degree. Then, sensitivity is lost with decreasing chamber size. We recommend a minimum chamber volume of 0.5 mL of the [[O2k-sV-Module]] or 2.0 mL for the operational volume of the standard [[O2k-Chamber]].<br />
<br />
<br />
=== Paradigm shift from mimimum to optimum O2k-Chamber volume ===<br />
<br />
:::: 1. Minimization of [https://wiki.oroboros.at/index.php/Chamber_volume chamber volume] represents a past paradigm, aming at high rates of oxygen consumption per volume. The advantage appears to be obvious, whereas the drawbacks are frequently overlooked (see below).<br />
:::: 2. Advancements of electronics, data acquisition and analysis, polarographic oxygen sensor specifications and chamber design made possible a superior approach, allowing for respirometric measurements at high dilution, as reviewed by [https://www.ncbi.nlm.nih.gov/pubmed/11718759?dopt=Abstract Gnaiger E (2001)]. In specifically designed mitochondrial respiration media, respiration is stable at high dilution, multiple substrate/inhibitor titrations are possible without oxygen depletion, and a low-oxygen regime may be chosen to prevent elevation of oxidative stress at air-level oxygen saturation. In contrast, micro-chambers are characterized by a high surface-to-volume ratio which hinders optimum stirring, increases unfavourable surface effects and oxygen-backdiffusion, and poses problems with accurate titrations and dilution effects of the sample. These potential - and mostly hidden - artefacts are avoided in high-resolution respirometry, using glass chambers, titanium stoppers, and avoiding teflon-coated stirrers or perspex (yielding high back-diffusion of oxygen).<br />
<br />
:::: Assume you have 0.1 mg mitochondrial protein for a respirometric assay. Approach (1) would lead you to search for a 100 µL volume respirometer, to maintain a classical 1 mg/mL protein concentration. In contrast, high-resolution respirometry allows for dilution of mitochondria to 0.02 mg/mL protein. Dilution of 0.1 mg mitochondrial protein in a 2 mL chamber yields an optimum concentration for multiple substrate/inhibitor titrations and kinetic measurements. The high-resolution approach of the Oroboros O2k offers the unique advantages of a versatile and ready-to-use system for studies in mitochondrial physiology and pathology.<br />
<br />
<br />
== Cleaning the glass O2k-Chamber ==<br />
<br />
:::: There are different kinds of contamination that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:<br />
::::* [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help, the biological contamination may be embedded in ''e.g.'' protein contamination and the glass chamber should be disassembled and cleaned as described below:<br />
::::* Protein contamination and other macroscopic deposits: After long term use, a whitish deposit can be formed on the glass walls of the O2k-Chamber. Additionally, small glass splinters (difficult to see) may become embedded in such deposit. A sign of this is a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the O2k and treated with concentrated hydrochloric acid (HCL) for at least an overnight period. Use a stirring plate and the O2k-Stirring bar to simultaneously clean the stirrer bar. <br />
::::* Contamination by hydrophobic inhibitors: The ideal counter agent for this case is pure ethanol.<br />
::::* Carry-over of inhibitors or uncouplers: It is recommended to wash the O2k-Chambers with cells, [[tissue homogenate | thom]] or [[Isolated_mitochondria|imt]] as the inhibitors will bind to the mitochondria. The samples must not be fresh but can be used after storage at -20°C, these are preferred due to their higher binding capacity. Fill the O2k-Chambers to the rim with the suspension and leave in the O2k-Chambers with inserted stoppers for at least 30 minutes. Follow this with a cleaning of the O2k-Chambers according to the DLPs provided with the DatLab software. ''See'' [http://bioblast.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS here].<br />
::::* Inhibitors may be introduced accidentally, one example being 70% ethanol used in hospital settings containing antiseptics. Such inhibitors may accumulate ''e.g.'' in plastic parts and inhibit subsequent experiments. See the [[Talk:MiPNet19.03_O2k-cleaning_and_ISS#Apparent_carry-over_of_CI-inhibitors_versus_contamination_in_chemicals_.28update:_solved.29| Discussion page]].<br />
<br />
=== Cleaning DLP ===<br />
<br />
[[DL-Protocols]] (DLP) should be used for cleaning the O2k-Chambers, stirrers and stoppers before and after each experimental use. A full list of Instrumental DL-Protocols for cleaning is displayed in the 'Protocols' menu ('Run DL-Protocol / Set O2 limit' window). ''See'' [Instrumental:_Browse_DL-Protocols_and_templates Browse Instrumental DL-Protocols for cleaning]<br />
<br />
[[File:DLP-Cleaning_window.png|700px|'''Instrumental DL-Protocols for cleaning''']]<br />
<br />
<br />
<br />
<br />
== O2k-Chamber assembly - possible problems ==<br />
<br />
:::: A properly assembled O2k-Chamber can remain in the O2k for extended periods of time. <br />
:::: It is recommended to disassemble, clean and reassemble the O2k-Chamber at least every 1 - 2 years, to avoid the chamber from getting stuck, to clean the glass properly, and to detect any possible damages.<br />
<br />
:::* After assembly, medium is leaking out of the chamber<br />
::::# Siphon off medium and disassemble the chamber.<br />
::::# Check for apparent damages of the glass chamber, the [[POS seal tip]] and O-rings on chamber holders and stoppers. If any damage is visible, replace the part.<br />
::::# Reassemble the chamber and proceed with an [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']].<br />
:::* Oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]<br />
:::* Unstable O<sub>2</sub> signal<br />
:::* Unexpected high or low O<sub>2</sub> signal<br />
::::# The possible contribution of chamber assembly is checked by removing the OroboPOS from the chamber and observing, if the signal changes to the expected values. - [[O2k-Open Support#Basic_problems_and_solutions |» Futher details]].<br />
::::# If the O2k signal responds as expected, reassemble the O2k-Chamber and proceed with an [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']].<br />
<br />
<br />
== Leaky chamber due to damaged OroboPOS-Holder ==<br />
<br />
:::* Please see: [https://www.bioblast.at/index.php/O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder O2k-OroboPOS-Holder MitoPedia]<br />
<br />
<br />
== O2k-Chamber disassembly ==<br />
:::* After siphoning off any medium from the O2k-Chamber, remove the [[OroboPOS-Connector]] and [[O2k-Chamber Holder]] from the O2k.<br />
:::* Insert your finger (with gloves on) into the chamber and then gently pull the glass chamber up.<br />
:::* '''Problem: Glass chamber stuck in the O2k''', and the [[O2k-Chamber]] cannot be removed. It remains stuck even after washing with water.<br />
::::# This may be caused by spilled medium acting as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. Then GENTLY screw in the sensor holder (not the sensor connector) to push the glass chamber upwards (as performed during the O2k-Chamber assembly). <br />
::::# If this does not work, increase the temperature to 40 °C (or slightly higher) for washing and continue with removing attempts.<br />
<br />
<br />
== Video support for O2k-Chamber service ==<br />
:::* Instructions on how to insert the O2k-Chamber and how to volume calibrate can be found here: [https://wiki.oroboros.at/index.php/O2k-Videosupport#POS-service_and_Chamber-service_.28all_series.29 Chamber service]<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-Chambers&diff=206083
O2k-Chambers
2020-08-12T10:22:05Z
<p>Antunes Diana: </p>
<hr />
<div>{{MitoPedia<br />
|description='''O2k-Chamber''': 16 mm inner diameter, Duran glass polished, with standard operation volume (''V'') of 2 mL (2 cm<sup>3</sup>). The [[O2k-sV-Module]] has a small chamber volume of 0.5 mL.<br />
|info=[[O2k-Chamber]]<br />
}}<br />
__TOC__<br />
<br />
{{Template:Technical support integrated}}<br />
<br />
<br />
== O2k-Chamber: paradigm shift in HRR ==<br />
[[File:Glass chamber.jpg|right|thumb|300px|The glass chamber and PVDF stirrer of the O2k.]]<br />
::::* The glass chamber and PVDF stirrer of the O2k.<br />
::::* O2k: Paradigm shift and features of high-resolution respirometry. MiPNet09.01. Including sole source information. Bioblast Link<br />
<br />
<br />
:::: Small experimental chamber volumes of 20-50 µL generate problems rather than providing resolution with small amounts of tissue samples, low numbers of cultured cells or few isolated mitochondria. The surface to volume ratio increases with decreasing chamber volume, thus various boundary effects entail larger errors at smaller volume, in particular oxygen diffusion. While the rate of oxygen depletion per unit amount of sample increases linearly with decreasing chamber volume, side effects may increase to a larger degree. Then, sensitivity is lost with decreasing chamber size. We recommend a minimum chamber volume of 0.5 mL of the [[O2k-sV-Module]] or 2.0 mL for the operational volume of the standard [[O2k-Chamber]].<br />
<br />
<br />
=== Paradigm shift from mimimum to optimum O2k-Chamber volume ===<br />
<br />
:::: 1. Minimization of [https://wiki.oroboros.at/index.php/Chamber_volume chamber volume] represents a past paradigm, aming at high rates of oxygen consumption per volume. The advantage appears to be obvious, whereas the drawbacks are frequently overlooked (see below).<br />
:::: 2. Advancements of electronics, data acquisition and analysis, polarographic oxygen sensor specifications and chamber design made possible a superior approach, allowing for respirometric measurements at high dilution, as reviewed by [https://www.ncbi.nlm.nih.gov/pubmed/11718759?dopt=Abstract Gnaiger E (2001)]. In specifically designed mitochondrial respiration media, respiration is stable at high dilution, multiple substrate/inhibitor titrations are possible without oxygen depletion, and a low-oxygen regime may be chosen to prevent elevation of oxidative stress at air-level oxygen saturation. In contrast, micro-chambers are characterized by a high surface-to-volume ratio which hinders optimum stirring, increases unfavourable surface effects and oxygen-backdiffusion, and poses problems with accurate titrations and dilution effects of the sample. These potential - and mostly hidden - artefacts are avoided in high-resolution respirometry, using glass chambers, titanium stoppers, and avoiding teflon-coated stirrers or perspex (yielding high back-diffusion of oxygen).<br />
<br />
:::: Assume you have 0.1 mg mitochondrial protein for a respirometric assay. Approach (1) would lead you to search for a 100 µL volume respirometer, to maintain a classical 1 mg/mL protein concentration. In contrast, high-resolution respirometry allows for dilution of mitochondria to 0.02 mg/mL protein. Dilution of 0.1 mg mitochondrial protein in a 2 mL chamber yields an optimum concentration for multiple substrate/inhibitor titrations and kinetic measurements. The high-resolution approach of the Oroboros O2k offers the unique advantages of a versatile and ready-to-use system for studies in mitochondrial physiology and pathology.<br />
<br />
<br />
== Cleaning the glass O2k-Chamber ==<br />
<br />
:::: There are different kinds of contamination that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:<br />
::::* [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help, the biological contamination may be embedded in ''e.g.'' protein contamination and the glass chamber should be disassembled and cleaned as described below:<br />
::::* Protein contamination and other macroscopic deposits: After long term use, a whitish deposit can be formed on the glass walls of the O2k-Chamber. Additionally, small glass splinters (difficult to see) may become embedded in such deposit. A sign of this is a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the O2k and treated with concentrated hydrochloric acid (HCL) for at least an overnight period. Use a stirring plate and the O2k-Stirring bar to simultaneously clean the stirrer bar. <br />
::::* Contamination by hydrophobic inhibitors: The ideal counter agent for this case is pure ethanol.<br />
::::* Carry-over of inhibitors or uncouplers: Inhibitors bind to the mitochondria, thus, it is recommended to wash the O2k-Chambers with '''cells, tissue or isolated mitochondria''' stored at -20°C. If [[Living cells]] (ce) are available, these are preferred due to their higher binding capacity. Fill the O2k-Chambers to the rim with the suspension and leave in the O2k-Chambers with inserted stoppers for at least 30 minutes. Follow this with a cleaning of the O2k-Chambers according to the DLPs provided with the DatLab software. ''See'' [http://bioblast.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS here].<br />
::::* Inhibitors may be introduced accidentally, one example being 70% ethanol used in hospital settings containing antiseptics. Such inhibitors may accumulate ''e.g.'' in plastic parts and inhibit subsequent experiments. See the [[Talk:MiPNet19.03_O2k-cleaning_and_ISS#Apparent_carry-over_of_CI-inhibitors_versus_contamination_in_chemicals_.28update:_solved.29| Discussion page]].<br />
<br />
=== Cleaning DLP ===<br />
<br />
[[DL-Protocols]] (DLP) should be used for cleaning the O2k-Chambers, stirrers and stoppers before and after each experimental use. A full list of Instrumental DL-Protocols for cleaning is displayed in the 'Protocols' menu ('Run DL-Protocol / Set O2 limit' window). ''See'' [Instrumental:_Browse_DL-Protocols_and_templates Browse Instrumental DL-Protocols for cleaning]<br />
<br />
[[File:DLP-Cleaning_window.png|700px|'''Instrumental DL-Protocols for cleaning''']]<br />
<br />
<br />
<br />
<br />
== O2k-Chamber assembly - possible problems ==<br />
<br />
:::: A properly assembled O2k-Chamber can remain in the O2k for extended periods of time. <br />
:::: It is recommended to disassemble, clean and reassemble the O2k-Chamber at least every 1 - 2 years, to avoid the chamber from getting stuck, to clean the glass properly, and to detect any possible damages.<br />
<br />
:::* After assembly, medium is leaking out of the chamber<br />
::::# Siphon off medium and disassemble the chamber.<br />
::::# Check for apparent damages of the glass chamber, the [[POS seal tip]] and O-rings on chamber holders and stoppers. If any damage is visible, replace the part.<br />
::::# Reassemble the chamber and proceed with an [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']].<br />
:::* Oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]<br />
:::* Unstable O<sub>2</sub> signal<br />
:::* Unexpected high or low O<sub>2</sub> signal<br />
::::# The possible contribution of chamber assembly is checked by removing the OroboPOS from the chamber and observing, if the signal changes to the expected values. - [[O2k-Open Support#Basic_problems_and_solutions |» Futher details]].<br />
::::# If the O2k signal responds as expected, reassemble the O2k-Chamber and proceed with an [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']].<br />
<br />
<br />
== Leaky chamber due to damaged OroboPOS-Holder ==<br />
<br />
:::* Please see: [https://www.bioblast.at/index.php/O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder O2k-OroboPOS-Holder MitoPedia]<br />
<br />
<br />
== O2k-Chamber disassembly ==<br />
:::* After siphoning off any medium from the O2k-Chamber, remove the [[OroboPOS-Connector]] and [[O2k-Chamber Holder]] from the O2k.<br />
:::* Insert your finger (with gloves on) into the chamber and then gently pull the glass chamber up.<br />
:::* '''Problem: Glass chamber stuck in the O2k''', and the [[O2k-Chamber]] cannot be removed. It remains stuck even after washing with water.<br />
::::# This may be caused by spilled medium acting as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. Then GENTLY screw in the sensor holder (not the sensor connector) to push the glass chamber upwards (as performed during the O2k-Chamber assembly). <br />
::::# If this does not work, increase the temperature to 40 °C (or slightly higher) for washing and continue with removing attempts.<br />
<br />
<br />
== Video support for O2k-Chamber service ==<br />
:::* Instructions on how to insert the O2k-Chamber and how to volume calibrate can be found here: [https://wiki.oroboros.at/index.php/O2k-Videosupport#POS-service_and_Chamber-service_.28all_series.29 Chamber service]<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-Chambers&diff=206081
O2k-Chambers
2020-08-12T10:16:00Z
<p>Antunes Diana: </p>
<hr />
<div>{{MitoPedia<br />
|description='''O2k-Chamber''': 16 mm inner diameter, Duran glass polished, with standard operation volume (''V'') of 2 mL (2 cm<sup>3</sup>). The [[O2k-sV-Module]] has a small chamber volume of 0.5 mL.<br />
|info=[[O2k-Chamber]]<br />
}}<br />
__TOC__<br />
<br />
{{Template:Technical support integrated}}<br />
<br />
<br />
== O2k-Chamber: paradigm shift in HRR ==<br />
[[File:Glass chamber.jpg|right|thumb|300px|The glass chamber and PVDF stirrer of the O2k.]]<br />
::::* The glass chamber and PVDF stirrer of the O2k.<br />
::::* O2k: Paradigm shift and features of high-resolution respirometry. MiPNet09.01. Including sole source information. Bioblast Link<br />
<br />
<br />
:::: Small experimental chamber volumes of 20-50 µL generate problems rather than providing resolution with small amounts of tissue samples, low numbers of cultured cells or few isolated mitochondria. The surface to volume ratio increases with decreasing chamber volume, thus various boundary effects entail larger errors at smaller volume, in particular oxygen diffusion. While the rate of oxygen depletion per unit amount of sample increases linearly with decreasing chamber volume, side effects may increase to a larger degree. Then, sensitivity is lost with decreasing chamber size. We recommend a minimum chamber volume of 0.5 mL of the [[O2k-sV-Module]] or 2.0 mL for the operational volume of the standard [[O2k-Chamber]].<br />
<br />
<br />
=== Paradigm shift from mimimum to optimum chamber volume ===<br />
<br />
:::: 1. Minimization of [https://wiki.oroboros.at/index.php/Chamber_volume chamber volume] represents a past paradigm, aming at high rates of oxygen consumption per volume. The advantage appears to be obvious, whereas the drawbacks are frequently overlooked (see below).<br />
:::: 2. Advancements of electronics, data acquisition and analysis, polarographic oxygen sensor specifications and chamber design made possible a superior approach, allowing for respirometric measurements at high dilution, as reviewed by [https://www.ncbi.nlm.nih.gov/pubmed/11718759?dopt=Abstract Gnaiger E (2001)]. In specifically designed mitochondrial respiration media, respiration is stable at high dilution, multiple substrate/inhibitor titrations are possible without oxygen depletion, and a low-oxygen regime may be chosen to prevent elevation of oxidative stress at air-level oxygen saturation. In contrast, micro-chambers are characterized by a high surface-to-volume ratio which hinders optimum stirring, increases unfavourable surface effects and oxygen-backdiffusion, and poses problems with accurate titrations and dilution effects of the sample. These potential - and mostly hidden - artefacts are avoided in high-resolution respirometry, using glass chambers, titanium stoppers, and avoiding teflon-coated stirrers or perspex (yielding high back-diffusion of oxygen).<br />
<br />
:::: Assume you have 0.1 mg mitochondrial protein for a respirometric assay. Approach (1) would lead you to search for a 100 µL volume respirometer, to maintain a classical 1 mg/mL protein concentration. In contrast, high-resolution respirometry allows for dilution of mitochondria to 0.02 mg/mL protein. Dilution of 0.1 mg mitochondrial protein in a 2 mL chamber yields an optimum concentration for multiple substrate/inhibitor titrations and kinetic measurements. The high-resolution approach of the Oroboros O2k offers the unique advantages of a versatile and ready-to-use system for studies in mitochondrial physiology and pathology.<br />
<br />
<br />
== Cleaning the glass chamber ==<br />
<br />
:::: There are different kinds of contamination that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:<br />
::::* [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help, the biological contamination may be embedded in ''e.g.'' protein contamination and the glass chamber should be disassembled and cleaned as described below:<br />
::::* Protein contamination and other macroscopic deposits: After long term use, a whitish deposit can be formed on the glass walls of the O2k-Chamber. Additionally, small glass splinters (difficult to see) may become embedded in such deposit. A sign of this is a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the O2k and treated with concentrated hydrochloric acid (HCL) for at least an overnight period. Use a stirring plate and the O2k-Stirring bar to simultaneously clean the stirrer bar. <br />
::::* Contamination by hydrophobic inhibitors: The ideal counter agent for this case is pure ethanol.<br />
::::* Carry-over of inhibitors or uncouplers: Inhibitors bind to the mitochondria, thus, it is recommended to wash the O2k-Chambers with '''cells, tissue or isolated mitochondria''' stored at -20°C. If [[Living cells]] (ce) are available, these are preferred due to their higher binding capacity. Fill the O2k-Chambers to the rim with the suspension and leave in the O2k-Chambers with inserted stoppers for at least 30 minutes. Follow this with a cleaning of the O2k-Chambers according to the DLPs provided with the DatLab software. ''See'' [http://bioblast.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS here].<br />
::::* Inhibitors may be introduced accidentally, one example being 70% ethanol used in hospital settings containing antiseptics. Such inhibitors may accumulate ''e.g.'' in plastic parts and inhibit subsequent experiments. See the [[Talk:MiPNet19.03_O2k-cleaning_and_ISS#Apparent_carry-over_of_CI-inhibitors_versus_contamination_in_chemicals_.28update:_solved.29| Discussion page]].<br />
<br />
=== Cleaning DLP ===<br />
<br />
[[DL-Protocols]] (DLP) should be used for cleaning the O2k-Chambers, stirrers and stoppers before and after each experimental use. A full list of Instrumental DL-Protocols for cleaning is displayed in the 'Protocols' menu ('Run DL-Protocol / Set O2 limit' window). ''See'' [Instrumental:_Browse_DL-Protocols_and_templates Browse Instrumental DL-Protocols for cleaning]<br />
<br />
[[File:DLP-Cleaning_window.png|700px|'''Instrumental DL-Protocols for cleaning''']]<br />
<br />
<br />
<br />
<br />
== O2k-Chamber assembly - possible problems ==<br />
<br />
:::: A properly assembled O2k-Chamber can remain in the O2k for extended periods of time. <br />
:::: It is recommended to disassemble, clean and reassemble the O2k-Chamber at least every 1 - 2 years, to avoid the chamber from getting stuck, to clean the glass properly, and to detect any possible damages.<br />
<br />
:::* After assembly, medium is leaking out of the chamber<br />
::::# Siphon off medium and disassemble the chamber.<br />
::::# Check for apparent damages of the glass chamber, the [[POS seal tip]] and O-rings on chamber holders and stoppers. If any damage is visible, replace the part.<br />
::::# Reassemble the chamber and proceed with an O2 sensor test.<br />
:::* Oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]<br />
:::* Unstable O2 signal<br />
:::* Unexpected high or low O2 signal<br />
::::# The possible contribution of chamber assembly is checked by removing the OroboPOS from the chamber and observing, if the signal changes to the expected values. - [[O2k-Open Support#Basic_problems_and_solutions |» Futher details]].<br />
::::# If the O2k signal responds as expected, reassemble the O2k-Chamber and proceed with an [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']] .<br />
<br />
<br />
== Leaky chamber due to damaged OroboPOS-Holder ==<br />
<br />
:::* Please see: [https://www.bioblast.at/index.php/O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder O2k-OroboPOS-Holder MitoPedia]<br />
<br />
<br />
== Chamber disassembly ==<br />
:::* After siphoning off any medium from the chamber, remove the [[OroboPOS-Connector]] and [[O2k-Chamber Holder]] from the O2k.<br />
:::* Insert your finger (with gloves on) into the chamber and then gently pull the glass chamber up.<br />
:::* '''Problem: Glass chamber stuck in the O2k''', and the [[O2k-Chamber]] cannot be removed. It remains stuck even after washing with water.<br />
::::# This may be caused by spilled medium acting as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. Then GENTLY screw in the sensor holder (not the sensor connector) to push the glass chamber upwards (as you do during chamber_assembly. <br />
::::# If this does not work, increase the temperature to 40 °C (or slightly higher) for washing and continue with removing attempts.<br />
<br />
<br />
== Video support for Chamber service ==<br />
:::* Instructions on how to insert the chamber and how to volume calibrate can be found here: [https://wiki.oroboros.at/index.php/O2k-Videosupport#POS-service_and_Chamber-service_.28all_series.29 Chamber service]<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-Chambers&diff=206080
O2k-Chambers
2020-08-12T10:14:03Z
<p>Antunes Diana: </p>
<hr />
<div>{{MitoPedia<br />
|description='''O2k-Chamber''': 16 mm inner diameter, Duran glass polished, with standard operation volume (''V'') of 2 mL (2 cm<sup>3</sup>). The [[O2k-sV-Module]] has a small chamber volume of 0.5 mL.<br />
|info=[[O2k-Chamber]]<br />
}}<br />
__TOC__<br />
<br />
{{Template:Technical support integrated}}<br />
<br />
<br />
== O2k-Chamber: paradigm shift in HRR ==<br />
[[File:Glass chamber.jpg|right|thumb|300px|The glass chamber and PVDF stirrer of the O2k.]]<br />
::::* The glass chamber and PVDF stirrer of the O2k.<br />
::::* O2k: Paradigm shift and features of high-resolution respirometry. MiPNet09.01. Including sole source information. Bioblast Link<br />
<br />
<br />
:::: Small experimental chamber volumes of 20-50 µL generate problems rather than providing resolution with small amounts of tissue samples, low numbers of cultured cells or few isolated mitochondria. The surface to volume ratio increases with decreasing chamber volume, thus various boundary effects entail larger errors at smaller volume, in particular oxygen diffusion. While the rate of oxygen depletion per unit amount of sample increases linearly with decreasing chamber volume, side effects may increase to a larger degree. Then, sensitivity is lost with decreasing chamber size. We recommend a minimum chamber volume of 0.5 mL of the [[O2k-sV-Module]] or 2.0 mL for the operational volume of the standard [[O2k-Chamber]].<br />
<br />
<br />
=== Paradigm shift from mimimum to optimum chamber volume ===<br />
<br />
:::: 1. Minimization of [https://wiki.oroboros.at/index.php/Chamber_volume chamber volume] represents a past paradigm, aming at high rates of oxygen consumption per volume. The advantage appears to be obvious, whereas the drawbacks are frequently overlooked (see below).<br />
:::: 2. Advancements of electronics, data acquisition and analysis, polarographic oxygen sensor specifications and chamber design made possible a superior approach, allowing for respirometric measurements at high dilution, as reviewed by [https://www.ncbi.nlm.nih.gov/pubmed/11718759?dopt=Abstract Gnaiger E (2001)]. In specifically designed mitochondrial respiration media, respiration is stable at high dilution, multiple substrate/inhibitor titrations are possible without oxygen depletion, and a low-oxygen regime may be chosen to prevent elevation of oxidative stress at air-level oxygen saturation. In contrast, micro-chambers are characterized by a high surface-to-volume ratio which hinders optimum stirring, increases unfavourable surface effects and oxygen-backdiffusion, and poses problems with accurate titrations and dilution effects of the sample. These potential - and mostly hidden - artefacts are avoided in high-resolution respirometry, using glass chambers, titanium stoppers, and avoiding teflon-coated stirrers or perspex (yielding high back-diffusion of oxygen).<br />
<br />
:::: Assume you have 0.1 mg mitochondrial protein for a respirometric assay. Approach (1) would lead you to search for a 100 µL volume respirometer, to maintain a classical 1 mg/mL protein concentration. In contrast, high-resolution respirometry allows for dilution of mitochondria to 0.02 mg/mL protein. Dilution of 0.1 mg mitochondrial protein in a 2 mL chamber yields an optimum concentration for multiple substrate/inhibitor titrations and kinetic measurements. The high-resolution approach of the Oroboros O2k offers the unique advantages of a versatile and ready-to-use system for studies in mitochondrial physiology and pathology.<br />
<br />
<br />
== Cleaning the glass chamber ==<br />
<br />
:::: There are different kinds of contamination that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:<br />
::::* [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help, the biological contamination may be embedded in ''e.g.'' protein contamination and the glass chamber should be disassembled and cleaned as described below:<br />
::::* Protein contamination and other macroscopic deposits: After long term use, a whitish deposit can be formed on the glass walls of the O2k-Chamber. Additionally, small glass splinters (difficult to see) may become embedded in such deposit. A sign of this is a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the O2k and treated with concentrated hydrochloric acid (HCL) for at least an overnight period. Use a stirring plate and the O2k-Stirring bar to simultaneously clean the stirrer bar. <br />
::::* Contamination by hydrophobic inhibitors: The ideal counter agent for this case is pure ethanol.<br />
::::* Carry-over of inhibitors or uncouplers: Inhibitors bind to the mitochondria, thus, it is recommended to wash the O2k-Chambers with '''cells, tissue or isolated mitochondria''' stored at -20°C. If [[Living cells]] (ce) are available, these are preferred due to their higher binding capacity. Fill the O2k-Chambers to the rim with the suspension and leave in the O2k-Chambers with inserted stoppers for at least 30 minutes. Follow this with a cleaning of the O2k-Chambers according to the DLPs provided with the DatLab software. ''See'' [http://bioblast.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS here].<br />
::::* Inhibitors may be introduced accidentally, one example being 70% ethanol used in hospital settings containing antiseptics. Such inhibitors may accumulate ''e.g.'' in plastic parts and inhibit subsequent experiments. See the [[Talk:MiPNet19.03_O2k-cleaning_and_ISS#Apparent_carry-over_of_CI-inhibitors_versus_contamination_in_chemicals_.28update:_solved.29| Discussion page]].<br />
<br />
=== Cleaning DLP ===<br />
<br />
[[DL-Protocols]] (DLP) should be used for cleaning the O2k-Chambers, stirrers and stoppers before and after each experimental use. A full list of Instrumental DL-Protocols for cleaning is displayed in the 'Protocols' menu ('Run DL-Protocol / Set O2 limit' window). ''See'' [https://wiki.oroboros.at/index.php/Instrumental:_Browse_DL-Protocols_and_templates Browse Instrumental DL-Protocols for cleaning]<br />
<br />
[[File:DLP-Cleaning_window.png|700px|'''Instrumental DL-Protocols for cleaning''']]<br />
<br />
<br />
<br />
<br />
== O2k-Chamber assembly - possible problems ==<br />
<br />
:::: A properly assembled O2k-Chamber can remain in the O2k for extended periods of time. <br />
:::: It is recommended to disassemble, clean and reassemble the O2k-Chamber at least every 1 - 2 years, to avoid the chamber from getting stuck, to clean the glass properly, and to detect any possible damages.<br />
<br />
:::* After assembly, medium is leaking out of the chamber<br />
::::# Siphon off medium and disassemble the chamber.<br />
::::# Check for apparent damages of the glass chamber, the [[POS seal tip]] and O-rings on chamber holders and stoppers. If any damage is visible, replace the part.<br />
::::# Reassemble the chamber and proceed with an O2 sensor test.<br />
:::* Oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]<br />
:::* Unstable O2 signal<br />
:::* Unexpected high or low O2 signal<br />
::::# The possible contribution of chamber assembly is checked by removing the OroboPOS from the chamber and observing, if the signal changes to the expected values. - [[O2k-Open Support#Basic_problems_and_solutions |» Futher details]].<br />
::::# If the O2k signal responds as expected, reassemble the O2k-chamber and proceed with an O2 sensor test.<br />
<br />
<br />
== Leaky chamber due to damaged OroboPOS-Holder ==<br />
<br />
:::* Please see: [https://www.bioblast.at/index.php/O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder O2k-OroboPOS-Holder MitoPedia]<br />
<br />
<br />
== Chamber disassembly ==<br />
:::* After siphoning off any medium from the chamber, remove the [[OroboPOS-Connector]] and [[O2k-Chamber Holder]] from the O2k.<br />
:::* Insert your finger (with gloves on) into the chamber and then gently pull the glass chamber up.<br />
:::* '''Problem: Glass chamber stuck in the O2k''', and the [[O2k-Chamber]] cannot be removed. It remains stuck even after washing with water.<br />
::::# This may be caused by spilled medium acting as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. Then GENTLY screw in the sensor holder (not the sensor connector) to push the glass chamber upwards (as you do during chamber_assembly. <br />
::::# If this does not work, increase the temperature to 40 °C (or slightly higher) for washing and continue with removing attempts.<br />
<br />
<br />
== Video support for Chamber service ==<br />
:::* Instructions on how to insert the chamber and how to volume calibrate can be found here: [https://wiki.oroboros.at/index.php/O2k-Videosupport#POS-service_and_Chamber-service_.28all_series.29 Chamber service]<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-Chambers&diff=206079
O2k-Chambers
2020-08-12T10:12:55Z
<p>Antunes Diana: </p>
<hr />
<div>{{MitoPedia<br />
|description='''O2k-Chamber''': 16 mm inner diameter, Duran glass polished, with standard operation volume (''V'') of 2 mL (2 cm<sup>3</sup>). The [[O2k-sV-Module]] has a small chamber volume of 0.5 mL.<br />
|info=[[O2k-Chamber]]<br />
}}<br />
__TOC__<br />
<br />
{{Template:Technical support integrated}}<br />
<br />
<br />
== O2k-Chamber: paradigm shift in HRR ==<br />
[[File:Glass chamber.jpg|right|thumb|300px|The glass chamber and PVDF stirrer of the O2k.]]<br />
::::* The glass chamber and PVDF stirrer of the O2k.<br />
::::* O2k: Paradigm shift and features of high-resolution respirometry. MiPNet09.01. Including sole source information. Bioblast Link<br />
<br />
<br />
:::: Small experimental chamber volumes of 20-50 µL generate problems rather than providing resolution with small amounts of tissue samples, low numbers of cultured cells or few isolated mitochondria. The surface to volume ratio increases with decreasing chamber volume, thus various boundary effects entail larger errors at smaller volume, in particular oxygen diffusion. While the rate of oxygen depletion per unit amount of sample increases linearly with decreasing chamber volume, side effects may increase to a larger degree. Then, sensitivity is lost with decreasing chamber size. We recommend a minimum chamber volume of 0.5 mL of the [[O2k-sV-Module]] or 2.0 mL for the operational volume of the standard [[O2k-Chamber]].<br />
<br />
<br />
=== Paradigm shift from mimimum to optimum chamber volume ===<br />
<br />
:::: 1. Minimization of [https://wiki.oroboros.at/index.php/Chamber_volume chamber volume] represents a past paradigm, aming at high rates of oxygen consumption per volume. The advantage appears to be obvious, whereas the drawbacks are frequently overlooked (see below).<br />
:::: 2. Advancements of electronics, data acquisition and analysis, polarographic oxygen sensor specifications and chamber design made possible a superior approach, allowing for respirometric measurements at high dilution, as reviewed by [https://www.ncbi.nlm.nih.gov/pubmed/11718759?dopt=Abstract Gnaiger E (2001)]. In specifically designed mitochondrial respiration media, respiration is stable at high dilution, multiple substrate/inhibitor titrations are possible without oxygen depletion, and a low-oxygen regime may be chosen to prevent elevation of oxidative stress at air-level oxygen saturation. In contrast, micro-chambers are characterized by a high surface-to-volume ratio which hinders optimum stirring, increases unfavourable surface effects and oxygen-backdiffusion, and poses problems with accurate titrations and dilution effects of the sample. These potential - and mostly hidden - artefacts are avoided in high-resolution respirometry, using glass chambers, titanium stoppers, and avoiding teflon-coated stirrers or perspex (yielding high back-diffusion of oxygen).<br />
<br />
:::: Assume you have 0.1 mg mitochondrial protein for a respirometric assay. Approach (1) would lead you to search for a 100 µL volume respirometer, to maintain a classical 1 mg/mL protein concentration. In contrast, high-resolution respirometry allows for dilution of mitochondria to 0.02 mg/mL protein. Dilution of 0.1 mg mitochondrial protein in a 2 mL chamber yields an optimum concentration for multiple substrate/inhibitor titrations and kinetic measurements. The high-resolution approach of the Oroboros O2k offers the unique advantages of a versatile and ready-to-use system for studies in mitochondrial physiology and pathology.<br />
<br />
<br />
== Cleaning the glass chamber ==<br />
<br />
:::: There are different kinds of contamination that can accumulate in the measuring chamber of the oxygraph and cause problems. All of them have to be treated in different ways:<br />
::::* [[Biological contamination]]: The ideal counter agent is 70% Ethanol with 30% water (NOT 100% Ethanol). If this does not help, the biological contamination may be embedded in ''e.g.'' protein contamination and the glass chamber should be disassembled and cleaned as described below:<br />
::::* Protein contamination and other macroscopic deposits: After long term use, a whitish deposit can be formed on the glass walls of the O2k-Chamber. Additionally, small glass splinters (difficult to see) may become embedded in such deposit. A sign of this is a jumping stirring bar or a stirring bar getting stuck. In this case the glass chamber should be removed from the O2k and treated with concentrated hydrochloric acid (HCL) for at least an overnight period. Use a stirring plate and the O2k-Stirring bar to simultaneously clean the stirrer bar. <br />
::::* Contamination by hydrophobic inhibitors: The ideal counter agent for this case is pure ethanol.<br />
::::* Carry-over of inhibitors or uncouplers: Inhibitors bind to the mitochondria, thus, it is recommended to wash the O2k-Chambers with '''cells, tissue or isolated mitochondria''' stored at -20°C. If [[Living cells]] (ce) are available, these are preferred due to their higher binding capacity. Fill the O2k-Chambers to the rim with the suspension and leave in the O2k-Chambers with inserted stoppers for at least 30 minutes. Follow this with a cleaning of the O2k-Chambers according to the DLPs provided with the DatLab software. ''See'' [http://bioblast.at/index.php/MiPNet19.03_O2k-cleaning_and_ISS here].<br />
::::* Inhibitors may be introduced accidentally, one example being 70% ethanol used in hospital settings containing antiseptics. Such inhibitors may accumulate ''e.g.'' in plastic parts and inhibit subsequent experiments. See the [[Talk:MiPNet19.03_O2k-cleaning_and_ISS#Apparent_carry-over_of_CI-inhibitors_versus_contamination_in_chemicals_.28update:_solved.29| Discussion page]].<br />
<br />
=== Cleaning DLP ===<br />
<br />
[[DL-Protocols]] (DLP) should be used for cleaning the O2k-Chambers, stirrers and stoppers before and after each experimental use. A full list of Instrumental DL-Protocols for cleaning is displayed in the 'Protocols' menu ('Run DL-Protocol / Set O2 limit' window). ''See'' [Instrumental:_Browse_DL-Protocols_and_templates Browse Instrumental DL-Protocols for cleaning]<br />
<br />
[[File:DLP-Cleaning_window.png|700px|'''Instrumental DL-Protocols for cleaning''']]<br />
<br />
<br />
<br />
<br />
== O2k-Chamber assembly - possible problems ==<br />
<br />
:::: A properly assembled O2k-Chamber can remain in the O2k for extended periods of time. <br />
:::: It is recommended to disassemble, clean and reassemble the O2k-Chamber at least every 1 - 2 years, to avoid the chamber from getting stuck, to clean the glass properly, and to detect any possible damages.<br />
<br />
:::* After assembly, medium is leaking out of the chamber<br />
::::# Siphon off medium and disassemble the chamber.<br />
::::# Check for apparent damages of the glass chamber, the [[POS seal tip]] and O-rings on chamber holders and stoppers. If any damage is visible, replace the part.<br />
::::# Reassemble the chamber and proceed with an O2 sensor test.<br />
:::* Oxygen leak of the chamber (strongly negative intercept in the [[instrumental background test]]<br />
:::* Unstable O2 signal<br />
:::* Unexpected high or low O2 signal<br />
::::# The possible contribution of chamber assembly is checked by removing the OroboPOS from the chamber and observing, if the signal changes to the expected values. - [[O2k-Open Support#Basic_problems_and_solutions |» Futher details]].<br />
::::# If the O2k signal responds as expected, reassemble the O2k-chamber and proceed with an O2 sensor test.<br />
<br />
<br />
== Leaky chamber due to damaged OroboPOS-Holder ==<br />
<br />
:::* Please see: [https://www.bioblast.at/index.php/O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder O2k-OroboPOS-Holder MitoPedia]<br />
<br />
<br />
== Chamber disassembly ==<br />
:::* After siphoning off any medium from the chamber, remove the [[OroboPOS-Connector]] and [[O2k-Chamber Holder]] from the O2k.<br />
:::* Insert your finger (with gloves on) into the chamber and then gently pull the glass chamber up.<br />
:::* '''Problem: Glass chamber stuck in the O2k''', and the [[O2k-Chamber]] cannot be removed. It remains stuck even after washing with water.<br />
::::# This may be caused by spilled medium acting as an adhesive between glass chamber and copper block. Wash with plenty of warm water and try to remove the chamber while the O2k is switched on and heated up to 37 °C. Then GENTLY screw in the sensor holder (not the sensor connector) to push the glass chamber upwards (as you do during chamber_assembly. <br />
::::# If this does not work, increase the temperature to 40 °C (or slightly higher) for washing and continue with removing attempts.<br />
<br />
<br />
== Video support for Chamber service ==<br />
:::* Instructions on how to insert the chamber and how to volume calibrate can be found here: [https://wiki.oroboros.at/index.php/O2k-Videosupport#POS-service_and_Chamber-service_.28all_series.29 Chamber service]<br />
<br />
<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=O2k hardware<br />
}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Oxygen_flux_-_instrumental_background&diff=206073
Oxygen flux - instrumental background
2020-08-12T08:54:54Z
<p>Antunes Diana: </p>
<hr />
<div>{{MitoPedia<br />
|abbr=''J''°<sub>O2</sub><br />
|description='''Instrumental background oxygen flux''', ''J''°<sub>O2</sub>, in a respirometer is due to oxygen consumption by the [[POS]], and oxygen diffusion into or out of the aqueous medium in the [[O2k-Chamber]]. It is a property of the instrumental system, measured in the range of experimental oxygen levels by a standardized instrumental O<sub>2</sub> background test. The oxygen regime from air saturation towards zero oxygen is applied generally in experiments with isolated mitochondria and living or permeabilized cells. To overcome oxygen diffusion limitation in permeabilized fibers and homogenates, an elevated oxygen regime is applied, requiring instrumental background test in the same range of elevated oxygen.<br />
|info=[[MiPNet14.06 Instrumental O2 background]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_RespPhysiol]]<br />
}}<br />
::::» See [[MiPNet14.06 Instrumental O2 background]] for experimental details of the instrumental background test.<br />
<br />
::::» See [[Instrumental:_Browse_DL-Protocols_and_templates]] for how to find the '''Template O2 background.xlsx''' (available with DatLab 7.4 software).<br />
<br />
::::» Compare [[Chemical background correction of oxygen flux]].<br />
__TOC__<br />
{{Technical support integrated}}<br />
<br />
<br />
[[File:O2k-QCS.jpg|right|400px|O2k-QCS]]<br />
== O<sub>2</sub> slope in the closed and open O2k-Chamber ==<br />
<br />
:::: Instrumental background correction eliminates errors by systemic flux compensation, automatically performed by [[DatLab]]. If no experimental background test has been performed, the system default values are used, which are ''a''°=-2.0 pmol/(s·mL) for the intercept at zero oxygen concentration, and ''b''°=0.025 for the slope of background flux as a function of oxygen concentration.<br />
<br />
:::: Automatic correction for the instrumental background oxygen flux is an essential standard in high resolution respirometry. At the same time an instrumental background experiment is the ultimate test for instrumental performance, evaluating chamber performance after completion of all elements of the [[Oxygen sensor test]]. The instrumental background oxygen flux measured at air saturation should reflect the theoretically predicted volume-specific oxygen consumption by the oxygen sensor. The actual agreement using experimental respiration medium provides at the same time a test that excludes microbial contamination of the medium or serves to evaluate any autoxidation processes in newly tested experimental media.<br />
<br />
:::: In an [[open chamber]] of the O2k the liquid phase in the chamber (aqueous medium) is in equilibrium with the atmosphere. All oxygen consumed by the polarographic oxygen sensor ([[POS]]) is immediately replaced from the atmosphere. The oxygen signal therefore has to be constant and the (negative) time derivative of the oxygen signal, called "O2 slope uncorr." in DatLab, has to be zero. The background corrected oxygen flux is meaningless for the open chamber situation. This is because the background correction at air saturation subtracts the consumption of oxygen by the sensor from the negative slope, when diffusion into and out of the chamber is zero at air saturation. Therefore, the background-corrected oxygen flux in the open chamber at air saturation is shown as a negative value. To avoid this apparent artefact, the "O2 slope uncorr." is selected to be shown while the chamber is open. Only Graph Layouts that display "O2 slope uncorr." are suitable for assessing the stability of the oxygen signal when the chamber is open. Such Layouts are: <br />
::::* 01 Calibration show Temp<br />
::::* 02 Calibration - Background<br />
<br />
:::: The observation of a zero flux with an open chamber is an important performance parameter. It indicates that thermal stability and equilibrium of oxygen between the gas and aqueous phases have been reached. Therefore no experiment should be started before a zero "O2 slope uncorr." has been reached with an open chamber. The suggested criterion for signal stability is a "O2 slope uncorr." between -1 pmol/(s mL) and + 1 pmol/(s mL).<br />
<br />
<br />
::::* First step - O2k Quality Control 1: »[[MiPNet06.03 POS-calibration-SOP]]«<br />
[[File:O2k-QCS.jpg|350px|O2k-QCS]] <br />
<br />
::::* Second step - O2k Quality Control 2<br />
<br />
[[Image:MiPNet14.06.jpg|frame|Instrumental background experiment, measuring oxygen flux without biological sample at four oxygen levels (left), and linear relation between instrumental background oxygen flux and oxygen concentration (right). Modified after: Gnaiger E (2001).]]<br />
<br />
<br />
== Standard operating procedure ==<br />
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-Chamber assembly has to be tested, described in detail as an O2k-SOP:<br />
:::: The '''O2k-chamber test''' provides quality control at an instrumental level beyond the O2 sensor test:<br />
::::# [[O2k-Chamber]] not properly assembled or broken.<br />
::::## [[OroboPOS-Holder]] not properly positioned.<br />
::::## [[O2k-Chamber Holder]] not properly positioned; V- and O-rings not properly mounted ([[V-ring\30-35-4.5 mm]], [[O-ring\Viton\18x2 mm]]).<br />
::::# [[Volume-Calibration Ring]] not properly positioned by chamber volume calibration.<br />
::::# [[O-ring\Viton\12x1 mm]] injured and must be replaced on the stopper.<br />
::::# [[Stopper\black PEEK\conical Shaft\central Port]] broken conical edge or O-ring not properly applied. <br />
::::# [[OroboPOS-Seal Tip]] leaky.<br />
::::# Experimental medium consumes oxygen due to microbial contamination.<br />
<br />
::::* Next step - when measuring cytochrome ''c'' oxidase activity: Autoxidation of ascorbate and TMPD causes a [[MiPNet06.06_ChemicalBackground |chemical background oxygen flux]]. DatLab provides real-time correction for instrumental and chemical background.<br />
=== Instrumental oxygen background test for permeabilized muscle fibers ===<br />
<br />
:::# While biopsy sampling and fiber preparation proceed: Perform air calibration in [[MiR06Cr]], then close the chamber to evaluate instrumental background at air saturation (c. 10 min): This is a quality control of the medium, important under field conditions, where medium preservation (sterility) may be less controlled than in the lab. <br />
:::# Elevate oxygen concentration to 450-500 µM with oxygen gas ([[Syringe\60 ml\Gas-Injection]]), close and after two to three min perform a [[stirrer test]] (using the automatic stirrer test function of DatLab). This is important, since the [[OroboPOS]] may have a different response time at elevated oxygen concentration. If the response time increases dramatically, then the sensor may even show a non-linear response to oxygen concentration at high oxygen levels. <br />
:::# Instrumental background: After 20 min, open the chamber and allow O<sub>2</sub> to drop to c. 350 µM, close for 20 min, open and drop O<sub>2</sub> to c. 250 µM (this should be the lowest experimental O<sub>2</sub> concentration). <br />
:::# Increase O<sub>2</sub> with H<sub>2</sub>O<sub>2</sub> injection (c. 2 µl) to 400 µM, measure for 15-20 min instrumental background, simulating a re-oxygenation during the experiment.<br />
:::# Increase O<sub>2</sub> with H<sub>2</sub>O<sub>2</sub> injection (c. 1 µl) to 450-500 µM, until the fibers are added, for equilibrating the instrument at high O<sub>2</sub>.<br />
:::# Addition of permeabilized fibers into the O2k-Chamber: » [[Permeabilized muscle fibers]].<br />
== Instrumental O2 DLP ==<br />
<br />
:::: Instrumental O2 DL-Protocols (DLP) are used for calibrations and instrumental quality control, typically without experimental sample in the incubation medium. All Instrumental DL-Protocols available can be found in C:\DatLab\DL-Protocols\Instrumental\ (default directory after installation of DatLab).<br />
<br />
:::: A general description of DL-Protocols (Instrumental DL-Protocols and SUIT DL-Protocols) is displayed in [[DL-Protocols]].<br />
:::: For instructions on how to upload different instrumental background test parameters (''i.e.'', intercept and slope) from an existing file ('background source'), ''see'': [[Flux_/_Slope#Slope_configuration_menu|'''Copy from file''' option in the '''Slope configuration''' window]]<br />
<br />
== Instrumental O2 background representative traces ==<br />
<br />
[[File:2017-05-17 P2-01 Instrumental O2 background TIP2k.jpg|400px|center]]<br />
<br />
== Troubleshooting ==<br />
=== If specifications given in the [[MiPNet14.06 Instrumental O2 background |Instrumental O<sub>2</sub> background test]] are not obtained === <br />
<br />
::::: Check components for locating the problem.<br />
::::# Check the stirring bars for any contamination.<br />
::::# Check the stoppers for the quality of the O-rings and the conical edges.<br />
::::# If no indications of a defect are observed, disassemble the O2k-chamber.<br />
::::# Check the [[glass chamber]] for contamination or for broken edges.<br />
::::# Clean the copper block of the O2k and reassemble the O2k-chamber.<br />
::::# Reassemble and clean the chambers: [[MiPNet19.03 O2k-cleaning and ISS]].<br />
::::# Perform an O2 sensor test and - if successful - an O2k-chamber test, using fresh incubation medium.<br />
::::# If the problem with the instrumental O<sub>2</sub> background remains in one chamber, switch stoppers between chambers A and B.<br />
::::# Perform an O2k-chamber test (the sensor test is not necessary).<br />
::::# If the problem with the instrumental O<sub>2</sub> background remains in the same chamber, switch glass chambers between the left and right side of the O2k.<br />
::::# Perform an O2 sensor test and - if successful - proceed with the O2k-chamber test.<br />
::::# If the problem with the instrumental O<sub>2</sub> background remains in the same chamber, switch sensors between the left and right chamber.<br />
=== Oxygen concentration increase at high oxygen regime is not reproducible between chambers after re-oxygenations with O<sub>2</sub> ===<br />
<br />
* Anonymous user; Heinrich Heine University Düsseldorf <br />
<br />
'''Question:'''<br />
During our run at high oxygen (~400 µM) the chamber won't exponentially oxygenate and won't reach the same level as the other chamber (which contains the same media and was oxygenated with the same syringe). The background calibration is correct as is the gain, stir speed, and temperature. <br />
It happens to our O2ks - but different chambers on random different days - and not consistently the same chamber or same O2k. It is usually the same user who processes our muscle biopsies, but I'm pretty sure it's some setting or mistake we're making and not a problem with the actual POS or program. Any help or suggestions are appreciated! The data is attached (2019-12-13).<br />
<br />
[[File:Ticket2020011631000015_High O2 Question_A.jpg|700px|center]]<br />
[[File:Ticket2020011631000015_High O2 Question_B.jpg|700px|center]]<br />
<br />
'''Answer:'''<br />
When you inject O<sub>2</sub> for the first time in both chambers there is an identical slope of the O<sub>2</sub> concentration line (although the increase in chamber B is slower). In this situation you need to wait for the desired O<sub>2</sub> concentration longer in chamber A than B (which is just fine, such O<sub>2</sub> increase can be slow since the OroboPOS may have a different response time at elevated oxygen concentration (pointing out the importance of applying correction for Instrumental O<sub>2</sub> background). Instead you have injected several times more O<sub>2</sub> without waiting long enough for reaching the desired concentration. In fact, that O<sub>2</sub> concentration is continuously increasing in chamber B even after the stirrer test.<br />
We recommend to close the chamber immediately after reaching the target concentration (~400 µM for pfi), which displaces the gas phase and stops the equilibration process.<br />
<br />
::::: Additionally, your observed issue can be as well related to one or more of the following experimental points:<br />
<br />
::::# Do you always open the stoppers using the [https://www.bioblast.at/index.php/Stopper-Spacer stopper-spacer] making sure that both chambers have the same fixed gas phase?<br />
::::# Do you check that you have a clear gas phase when you look inside the chamber and not several 'bubbles'?<br />
:::: Another general points that could lead to dissimilar increase in the O<sub>2</sub> concentration (although not applicable in your case):<br />
<br />
::::# Do you inject O2 in the order of 1<sup>st</sup> chamber A (good chamber) followed by chamber B (bad chamber)? Do you always take fresh O<sub>2</sub> before injection between each chamber or do you use the remaining O<sub>2</sub> from the 1<sup>st</sup> injection?<br />
::::# Do you inject the same amount of O<sub>2</sub>?<br />
<br />
(...)<br />
=== Linear regressions values (intercept - a° and the slope - b°) are very different than the expected values ===<br />
<br />
* '''Customer ID''': [https://wiki.oroboros.at/index.php/US_OH_Cleveland_Hoppel_CL US_OH_Cleveland_Hoppel_CL]<br />
<br />
'''Question:'''<br />
We performed a high instrumental O2 background and noticed that somehow the values seemed lower than previously although the O2k-trace looks fine. The data is attached (2019-12-03).<br />
<br />
[[File:2019-12-03 HIGHO2 AB-01.png|700px|left]]<br />
<br />
[[File:2019-12-03 HIGHO2 AB-01 B.png|700px|left]]<br />
<br />
[[File:Lin regression A.png|250px|centre]]<br />
<br />
[[File:Lin regression B.png|250px|centre]]<br />
:<br />
:<br />
:<br />
<br><br />
<br><br />
<br><br />
<br><br />
<br />
'''Answer:'''<br />
Below you can find the stepwise analysis of your attached data.<br />
<br />
For obtaining the background linear regression: In the window 'O2 Background correction' select only the marks J°1h, J°2h, J°3h and J°4h to calculate the parameters Intercept, ''a°'' and Slope, ''b°'' and click 'Calibrate'.<br />
{| class="wikitable"<br />
! colspan="2" style="font-weight:bold;" | Default parameters of Instrumental high O2 background_TIP2k.DLP<br />
|-<br />
| style="text-align: center; background-color:#efefef;" | Mark name<br />
| style="text-align: center; background-color:#efefef;" | O2 concentration [µM]<br />
|-<br />
| style="text-align: center;" | J°1h<br />
| style="text-align: center;" | ~ 400<br />
|-<br />
| style="text-align: center;" | J°2h<br />
| style="text-align: center;" | ~ 370<br />
|-<br />
| style="text-align: center;" | J°3h<br />
| style="text-align: center;" | ~ 320<br />
|-<br />
| style="text-align: center;" | J°4h<br />
| style="text-align: center;" | ~ 270<br />
|-<br />
| colspan="2" style="text-align: center; font-weight:bold;" | Your applied parameters of Instrumental high O2 background (attached DLP)<br />
|-<br />
| style="text-align: center; background-color:#efefef;" | Mark name<br />
| style="text-align: center; background-color:#efefef;" | O2 concentration [µM]<br />
|-<br />
| style="text-align: center;" | your J°1h mark (02)<br />
| style="text-align: center;" | ~ 432<br />
|-<br />
| style="text-align: center;" | your J°2h mark (03)<br />
| style="text-align: center;" | ~ 351<br />
|-<br />
| style="text-align: center;" | your J°3h mark (04)<br />
| style="text-align: center;" | ~ 235<br />
|-<br />
| style="text-align: center;" | your J°4h mark (05)<br />
| style="text-align: center;" | ~ 154<br />
|}<br />
<br />
<br />
By selecting only the marks in the high oxygen regime (only the marks J°1h, J°2h, J°3h and J°4h) and according to the default O2 concentration range for the calibration '''Instrumental high O2 background_TIP2k.DLP''', ''i.e.'', de-selecting your mark 05 (which is an outlier from the guidelines: O2 concentration [µM] ~ 154) you can see that your 'a°' values approach the right values of y= -7.6 + 0.0352.<br />
<br />
Additionally, I would like to highlight the advantage of including:<br />
<br />
* the QC for assuring a contamination-free incubation medium - ''close O2k-Chamber'': '''Quality control e''' in [[Oxygen_sensor_test#Figure_2._POS_Quality_control_using_the_DatLab_protocol_.28MiPNet06.03_POS-calibration-SOP.29 | Oxygen sensor test]].<br />
* default parameters under F7 (should be set up prior to O2k connection to the DatLab software): data recording interval: 2.0 seconds; Gain 1; Temperature: 25 or 37 ° C (if overnight run 25° C, otherwise 37 ° C).<br />
<br />
=== Zero oxygen concentration reached but 'Instrumental_O2_background_TIP2k.DLP' is not finished ===<br />
<br />
:::* - ''See'': [[Dithionite#Zero_oxygen_concentration_reached_but_.27Instrumental_O2_background_TIP2k.DLP.27_is_not_finished |O2k open support case]]<br />
<br />
<br />
=== Unstable O2 slope neg. in instrumental background test ===<br />
:::* '''Customer ID''': anonymous<br />
:::: '''Problem:''' The O<sub>2</sub> instrumental background DLD file contains a drop in the O2 slope neg. after the Dithionite titration event '''P003'''. Setting the mark '''J°4''' at this point results in an intercept ''a°'' of -5.5782. See data below (2019-12-17).<br />
<br />
[[File: 2019-12-17 P1-O1 background calibration 2.png |700px]] <br />
<br />
:::: '''Solution:''' According to the DLD file, the mark '''J°4''' immediately after the event '''P003''' is set where the drop in O2 slope neg. occurs. Such a pattern can be explained by convective oxygen backdiffusion (for details, see [https://wiki.oroboros.at/images/4/41/Gnaiger_2008_POS.pdf Gnaiger 2008 POS] and [[O2k-pH_ISE-Module#Instrumental_background_oxygen_flux]]), which may occur when medium collects in the receptacle of the stopper, such that the liquid phase extends above the level of the capillary of the stopper. This liquid phase equilibrates with room temperature and thus may initiate a convective flow down the capillary in exchange of medium equilibrated at the higher experimental temperature. This is why [[TIP2k Filter Papers]] are used, to prevent such a liquid phase from accumulating. Care must be taken to place the TIP2k Filter Papers correctly within the stopper. The marks should always be set on the stable section of the slope (''i.e.'' in this case before the decline of O2 slope neg.). In case of any doubt, it is strongly recommended to repeat the instrumental O<sub>2</sub> background test, taking care of the position of the [[TIP2k Filter Papers]]. Additionally, it is better to use the layout ''02-Calibration Background'' for a better visualization of the plots, which helps to set the marks properly on stable phases of O2 slope neg.<br />
<br />
== References ==<br />
<br />
:::* Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - [[Gnaiger_1995_J_Bioenerg_Biomembr |»Bioblast link«]]<br />
:::* Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - [[Gnaiger_2001_Respir_Physiol |»Bioblast link«]]<br />
:::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. - [[Gnaiger_2008_POS |»Bioblast link«]]<br />
:::* Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - [[Doerrier 2018 Methods Mol Biol |»Bioblast link«]]<br />
<br />
[[File:Expand.png|right|45px |Click to expand or collaps]]<br />
<div class="toccolours mw-collapsible mw-collapsed"><br />
:::[[File:Questions.jpg|left|24px |Explanation of terms]] '''» Keywords''' - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>><br />
<div class="mw-collapsible-content"><br />
::: '''Instrument'''<br />
::::» [[O2k-FluoRespirometer]]<br />
::::» [[DatLab]]<br />
::::» [[O2k-Chamber]]<br />
::::» [[MiPNet19.18E O2 flux analysis - DatLab 6]]<br />
::::» [[MiPNet19.03 O2k-cleaning and ISS |O2k-Protocols SOP: O2k-cleaning]]<br />
<br />
::: '''Concept'''<br />
::::» [[Concentration]]<br />
::::» [[high-resolution respirometry]]<br />
::::» [[Instrumental background oxygen flux]]<br />
::::» [[MitoFit Quality Control System]]<br />
::::» [[Oxygen |Oxygen, dioxygen, O<sub>2</sub>]]<br />
</div><br />
</div><br />
<br />
<br />
{{MitoPedia concepts<br />
|mitopedia concept=MiP concept<br />
}}<br />
{{MitoPedia methods<br />
|mitopedia method=Respirometry<br />
}}<br />
{{MitoPedia O2k and high-resolution respirometry<br />
|mitopedia O2k and high-resolution respirometry=DatLab, Oroboros QM<br />
}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=205963
Template talk:Next oroboros ecosystem agenda
2020-08-10T12:26:22Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
*'''Aug-20 Thr'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to so with the [[Q-Module]] that we are developing as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project. <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
<br />
Suggestion 2:<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to so with the new module that we are developing as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Aug-25 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange by [[ANT]] using [[MgG]] with the O2k''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Aug-18 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration in PBMCs of major depression patients'''<br><br />
:from the O2k-Network »[[DE Ulm Karabatsiakis A]]« and »[[DE Ulm Radermacher P]]«<br />
*Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. [[Karabatsiakis 2014 Transl Psychiatry |»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
* '''Aug-17 Mo'''<br />
::Suggestion 1:<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you observe a high O2 flux in the closed chamber prior to addition of sample? '''<br><br />
This might be due to [[biological contamination]] of the O2k-chamber. See how to clean it properly. <br><br />
- [[O2k-Chamber MitoPedia#Cleaning the glass chamber]] and »[[MiPNet19.03 O2k-cleaning and ISS]]<br />
|}<br />
<br />
<br />
::Suggestion 2:<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 14:26, 10 August 2020 (CEST)<br />
::::1) I would use ‘slope of the oxygen signal (O2 slope neg.)’ instead of ‘O2 flux’ to have consistency between the terminology used in both DL7.4 and Mitopedia-DatLab;<br />
::::2) Would it be better to already give an answer in the alert to keep the customer’ attention?;<br />
::::3) Edit links name and added '»' between links. <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that a slope of the oxygen signal (O<sub>2</sub> slope neg.) > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed chamber may be due to [[biological contamination]] in the medium or O2k-Chamber? '''<br><br />
See how to clean the O2k-Chamber. <br><br />
- » [[O2k-Chamber MitoPedia#Cleaning the glass chamber | Cleaning the O2k-Chamber]]« and »[[MiPNet19.03 O2k-cleaning and ISS]]«<br />
|}<br />
<br />
::Suggestion 3:<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you observe an increased slope of the oxygen signal (O<sub>2</sub> slope neg.) > 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> in the closed chamber in the absence of a biological sample?''' <br><br />
This may be due to [[biological contamination]] in the medium or the O2k-Chamber. <br><br />
- » [[O2k-Chamber MitoPedia#Cleaning the glass chamber | Cleaning the O2k-Chamber]]« and »[[MiPNet19.03 O2k-cleaning and ISS]]««<br />
|}<br />
<br />
* '''Aug-24 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Can NADH be directly used as substrate in respirometry? '''<br><br />
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for mitochondrial respiration since NADH is not permeable through the [[mitochondrial inner membrane]] (mtIM). The mtIM should be intact for respirometry experiments. <br><br />
- [[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide ]]<br />
|}<br />
OR<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Warning: loss of data]]<br />
|'''Can NADH be directly used as substrate in respirometry? '''<br><br />
No, you cannot use NADH as a [[NADH Electron transfer-pathway state|NADH-linked]] substrate for mitochondrial respiration since NADH is not permeable through the [[mitochondrial inner membrane]] (mtIM). The mtIM should be intact for respirometry experiments. <br><br />
- [[Nicotinamide_adenine_dinucleotide|Nicotinamide adenine dinucleotide ]]<br />
|}<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Closed_chamber&diff=205959
Closed chamber
2020-08-10T11:50:55Z
<p>Antunes Diana: </p>
<hr />
<div>{{Technical support}}<br />
{{MitoPedia without banner<br />
|abbr=C<br />
|description=The O2k-Chamber can be used as a [[closed system]] or [[open system]]. Gas bubbles must be avoided.<br />
|info=<br />
}}<br />
<br />
:::: '''Closed chamber''' means that the chamber and the capillary of the stopper is filled with liquid and no air space or bubble is present in the chamber. For closing the chamber without a bubble an excessive amount of liquid has to be present in the chamber (2.5 mL for 2 mL chamber). The conical shape of the standard stopper facilitates bubble-free closing of the chamber with stirrers switched on - the bubbles will escape through the capillary. If some bubble remains in the chamber, the capillary should be relieved by inserting long needle into the capillary. After closing the chamber the excessive liquid should be aspirated from the top of the stopper.<br />
:::: The procedure of bubble-free closing closing a chamber with a multisensor stopper is described in the manual for ion selective electrodes [[MiPNet15.03]].<br />
<br />
:::: See also: [[Open chamber]]<br />
<br />
{{Template:Keywords: Oxygen signal}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPediaAlert2020_Archive&diff=205927
MitoPediaAlert2020 Archive
2020-08-10T06:14:51Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
== Aug 2020 ==<br />
* '''Aug-13 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Photosynthesis efficiency is reduced by 25% in plants and algae due the metabolic process called photorespiration. <br><br />
- »'''[[Photorespiration]]'''«<br />
|}<br />
----<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|}<br />
<br />
<br />
== Previous MitoPedia Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
----<br />
* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
----<br />
<br />
* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
----<br />
* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
----<br />
* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
<br />
== Jun 2020 ==<br />
* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
<br />
* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
<br />
* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_Red_method Incompatible substrates]'''«<br />
|}<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
<br />
* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
<br />
<br />
=== May 2020 ===<br />
<br />
* '''May-28 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
<br />
* '''May-21 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
|As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
* '''May-14 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
<br />
* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''<br />
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br><br />
- Learn more about - »'''[[Malate-anaplerotic pathway control state|malate-anaplerotic pathway control state]]'''« <br><br />
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« <br><br />
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
=== Apr 2020 ===<br />
<br />
* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]]. <br><br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
<br><br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. <br><br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
<br><br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br><br />
- »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
<br><br />
* '''Apr-08 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. <br><br />
- »'''[[SUIT-010 |SUIT-010]]'''«<br />
|}<br />
<br><br />
* '''Apr-02 Th''' <br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' <br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-16 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. <br><br />
- »'''[[SUIT-003 |SUIT-003]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. <br><br />
- »'''[[Blebbistatin]]'''«<br />
|}<br />
<br><br />
* '''Mar-06 Fr'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br><br />
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? <br><br />
- »'''[[Rapamycin]]'''«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-26 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. <br><br />
- »'''[[Noncoupled respiration]]'''«<br />
|}<br />
<br><br />
* '''Feb-24 Mo'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| More research is needed on the impact of viral infection on mitochondrial function. <br><br />
- »'''[[Viruses and mitochondrial medicine]]'''«<br />
|}<br />
<br><br />
* '''Feb-19 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. <br><br />
- »'''[[Spermidine]]'''«<br />
|}<br />
<br><br />
* '''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Why do we fight gas bubbles? <br><br />
- Dive deep into »'''[[Oxygen solubility]]'''«<br />
|}<br />
<br><br />
* '''Feb-12 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| What do metformin, melatonin and other bioactive mitObesity compounds have in common? <br><br />
- »'''[[MitObesity drugs]]'''«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-16 Th'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Oxygen flux]]''' <br><br />
- which SI units should be used?<br />
|}<br />
<br><br />
<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Did you ever think that units for body mass could be an issue? <br><br />
- »'''[[Body mass]]'''«<br />
|}<br />
<br><br />
* '''Jan-08 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-PublicationAlert2020_Archive&diff=205926
O2k-PublicationAlert2020 Archive
2020-08-10T06:14:03Z
<p>Antunes Diana: </p>
<hr />
<div>{{Template:OROBOROS Slogan}}<br />
::::<big>» Listed in '''[[Oroboros Ecosystem Agenda 2020]]'''</big><br />
</big>__TOC__<br />
<br />
== Aug 2020 ==<br />
* '''Aug-14 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br><br />
: from the O2k-Network »[[NZ Auckland Hickey AJ]]« <br><br />
* Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Aug-11 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring'''<br><br />
:from the O2k-Network »[[DE Bremerhaven Mark FC]]«<br />
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |»Bioblast link«]]<br />
|}<br />
----<br />
* '''Aug-04 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br><br />
:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]« <br />
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]<br />
|}<br />
<br />
<br />
<br />
<br />
== Previous O2k-Publication Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-collapsed"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-28 Tu''' - World Hepatitis Day<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Hepatitis C virus protein expression is related to changes in mitochondrial respiration'''<br><br />
:from the O2k-Network »[[IT Foggia Capitanio N]]« <br />
*Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60. [[Ripoli 2009 J Virol|»Bioblast link«]]<br />
|}<br />
----<br />
<br />
* '''Jul-21 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br><br />
:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]« <br />
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. [[Lantier 2020 Diabetes|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Jul-14 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br><br />
:from the O2k-Network »[[BR Ribeirao Preto Alberici LC]]« <br />
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-07 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br><br />
:from the O2k-Network »[[FR Lille Duez H]]« <br />
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Jul-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br><br />
: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br><br />
* Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]<br />
|}<br />
<br />
=== Jun 2020 ===<br />
* '''Jun-02 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br><br />
: from the O2k-Network »[[AT Innsbruck Gnaiger E]]« , »[[CA Rimouski Blier PU]]« and »[[CA Edmonton Lemieux H]]« <br />
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jun-12 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ10 in the platelets of CKD patients''' <br><br />
: from the O2k-Network » [[ SK Bratislava Sumbalova Z]] « <br><br />
* Gvozdjáková A, Sumbalová Z, Kucharská J, Komlósi M, Rausová Z, Vančová O, Számošová M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q10 and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|»Bioblast link«]]<br />
<br />
|}<br />
----<br />
* '''Jun-16 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br><br />
:from the O2k-Network »[[CA Montreal Hepple RT]]« <br />
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jun-23 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br><br />
:from the O2k-Network »[[IT Verona Calabria E]]« <br />
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jun-30 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]« <br />
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]<br />
|}<br />
=== May 2020 ===<br />
<br />
* '''May-29 Fr'''<br />
:{|<br />
<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br><br />
: from the O2k-Network »[[BR Sao Paulo Silber AM]]« <br />
*Alberto Luévano-Martínez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. [[Alberto Luevano-Martinez 2020 FEBS Lett|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
<br />
* '''May-26 Tu''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br><br />
: from the O2k-Network »[[ AT Innsbruck Gnaiger E]]« and »[[ AT Innsbruck Oroboros]]« <br><br />
* Schöpf B, Weissensteiner H, Schäfer G, Fazzini F, Charoentong P, Naschberger A, Rupp B, Fendt L, Bukur V, Giese I, Sorn P, Sant’Anna-Silva AC, Iglesias-Gonzalez J, Sahin U, Kronenberg F, Gnaiger E, Klocker H (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. [[Schoepf 2020 Nat Commun|»Bioblast link«]]<br />
<br />
|}<br />
<br />
----<br />
* '''May-19 Tu''' <br />
:{|<br />
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''«<br><br />
: from the O2k-Network »[[Charles Anne-Laure]]« »[[Geny Bernard]]«<br />
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|»Bioblast link«]]<br />
|}<br />
<br />
* '''May-17 Su''' World Hypertension Day <br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br><br />
: from the O2k-Network »[[CZ Prague Houstek J]]« and »[[CZ Prague Kalous M]]«<br />
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|»Bioblast link«]]<br />
|}<br />
<br />
* '''May-15 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br><br />
: from the O2k-Network » [[ FI Helsinki Jacobs HT]]« , »[[ DE Magdeburg Gellerich FN]]« , »[[FI Tampere Dufour E]]« , »[[DE Magdeburg Debska-Vielhaber G]]« and »[[DE Frankfurt Wittig I]]«<br><br />
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet M, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich Frank N, Schlueter KD, Jacobs HT(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]<br />
|}<br />
<br />
<br />
* '''May-12 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Mitochondria - a driving force in adipocyte differentiation''' <br><br />
: from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br><br />
* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''May-05 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Expression of alternative oxidase prevents reverse electron transfer formation in heart mitochondria isolated from mouse''' <br><br />
: from the O2k-Network »[[FI Helsinki Jacobs HT]]« »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«<br><br />
* Szibor M, Gainutdinov T, Fernandez-Vizarra E, Dufour E, Gizatullina Z, Debska-Vielhaber G, Heidler J, Wittig I, Viscomi C, Gellerich FN, Moore AL (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]<br />
|}<br />
<br />
=== Apr 2020 ===<br />
* '''Apr-28 Tu''' <br />
<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The mitochondrial gas pedal: Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake''' <br><br />
: from the O2k-Network » [[FI Helsinki Jacobs HT]] « » [[DE Magdeburg Gellerich FN]] « and »[[DE Magdeburg Debska-Vielhaber G]]«<br><br />
* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. [[Szibor 2020 J Biol Chem|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-20 Mo''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''The effect of respiration buffer composition on mitochondrial metabolism and function''' <br><br />
: from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«<br><br />
* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-15 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies''' <br><br />
: from the O2k-Network »[[UY Montevideo Radi R]]«<br><br />
* García-Roche M, Casal A, Carriquiry M, Radi R, Celia Quijano C, Cassina A (2018) Respiratory analysis of coupled mitochondria in cryopreserved liver biopsies. Redox Biol 17:207-12. - [[Garcia-Roche_2018_Redox_Biol|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-06 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Physical activity has positive effects on mitochondria from patients with occupational burnout''' <br><br />
: from the O2k-Network »[[CH Basel Eckert A]]«<br><br />
* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''<br><br />
: from the O2k-Network »[[DE Nuthetal Klaus S]]«<br><br />
* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - [[Ost 2020 EMBO Rep |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Apr-01 We''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria'''<br><br />
: from the O2k-Network »[[FR Angers Gueguen N]]« and »[[BE Leuven Spinazzi M]]«<br><br />
* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|»Bioblast link«]]<br />
|}<br />
<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-20 Fr'''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''High-resolution respirometry in human prostate cancer biopsies and mtDNA analyzed in samples recovered from the O2k-chamber'''<br><br />
: from the O2k-Network »[[AT Innsbruck Oroboros]]«<br><br />
* Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 2020-03-20 [Epub ahead of print]. - [[Schoepf_2020_Nat_Commun |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-12 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis'''<br><br />
: from the O2k-Network »[[UK London Duchen MR]]«<br><br />
* Warne J, Pryce G, Hill J, Shi X, Lennerås F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-10 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Muscular exercise for training adipose tissue mitochondria'''<br><br />
: from the O2k-Network »[[DK Copenhagen Larsen S]]«<br><br />
* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-09 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Protein-specific mitochondrial respiratory capacity is higher in women than men'''<br><br />
: from the O2k-Network »[[SE Stockholm Cardinale DA]]«<br><br />
* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-04 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets'''<br><br />
: from the O2k-Network »[[US FL Orlando Goodpaster BH]]«<br><br />
* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Rapamycin suppresses senescence in galactose versus glucose cell culture'''<br><br />
: from the O2k-Network »[[US PA Philadelphia Orynbayeva Z]]«<br><br />
* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Mar-02 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction''' <br><br />
: from the O2k-Network »[[CN Chongqing Zhu Z]]«<br />
* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |»Bioblast link«]]<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-27 Th''' <br />
<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase''' <br><br />
: from the O2k-Network »[[CZ Prague Houstek J]]« <br><br />
* Pajuelo-Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |»Bioblast link«]]<br />
|}<br />
<br><br />
* '''Feb-25 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''An active lifestyle protects cardiac mitochondria''' <br><br />
: from the O2k-Network »[[KR Busan Han J]]« - »[[KR Incheon Kwak HB]]« <br><br />
* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis''' <br><br />
: from the O2k-Network »[[US MI Ann Arbor Beard DA]]« <br><br />
* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. - [[Tyrrell 2019 Circ Res |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Feb-05 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease''' <br><br />
: from the O2k-Network »[[US FL Boca Raton Khamoui AV]]« <br><br />
* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Feb-03 Mo''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' <br><br />
: from the O2k-Network »[[US AR Little Rock Boersheim E]]« <br><br />
* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<br />
*'''Jan-28 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration of blood cells and cardiovascular disease''' <br><br />
: from the O2k-Network »[[FR Strasbourg Zoll J]]« <br><br />
* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - [[Alfatni 2020 J Clin Med |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Jan-18 Sa'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations''' <br><br />
: from the O2k-Network »[[CZ Prague Krajcova A]]« <br><br />
* Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Jan-17 Fr''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-close.jpg|left|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]]<br />
|<big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. <br><br />
: [[Scandurra_2010_Adv_Exp_Med_Biol |»Bioblast link«]]<br />
|}<br />
<br><br />
*'''Jan-15 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers''' <br><br />
: from the O2k-Network »[[AU Melbourne Stepto NK]]« <br><br />
* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J 34:2978-86. - [[Jacques 2020 FASEB J |»Bioblast link«]]<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Current_week_ecosystem_agenda&diff=205925
Template:Current week ecosystem agenda
2020-08-10T06:09:13Z
<p>Antunes Diana: </p>
<hr />
<div> 2020 Week 33<br />
<br />
----<br />
* '''Aug-10 Mo''' <br />
<br />
----<br />
* '''Aug-11 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Ocean warming affects mitochondrial ET capacity in developing eggs of Atlantic herring'''<br><br />
:from the O2k-Network »[[DE Bremerhaven Mark FC]]«<br />
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |»Bioblast link«]]<br />
|}<br />
<br />
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* '''Aug-12 We'''<br />
<br />
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* '''Aug-13 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Photosynthesis efficiency is reduced by 25% in plants and algae due the metabolic process called photorespiration. <br><br />
- »'''[[Photorespiration]]'''«<br />
|}<br />
----<br />
* '''Aug-14 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br><br />
: from the O2k-Network »[[NZ Auckland Hickey AJ]]« <br><br />
* Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|»Bioblast link«]]<br />
|}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Next_oroboros_ecosystem_agenda&diff=205924
Template:Next oroboros ecosystem agenda
2020-08-10T06:08:48Z
<p>Antunes Diana: </p>
<hr />
<div><br />
<br />
<br />
<br />
2020 Week 34<br />
<br />
* '''Aug-17 Mo''' <br />
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* '''Aug-18 Tu'''<br />
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* '''Aug-19 We'''<br />
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* '''Aug-20 Th'''<br />
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* '''Aug-21 Fr'''<br />
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* '''Aug-22 Sa'''<br />
<br />
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* '''Aug-23 Su''' <br />
<br />
2020 Week 35<br />
<br />
* '''Aug-24 Mo''' <br />
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* '''Aug-25 Tu'''<br />
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* '''Aug-26 We''' <br />
<br />
----<br />
* '''Aug-27 Th''' <br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
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* '''Aug-28 Fr'''<br />
<br />
2020 Week 36<br />
<br />
* '''Aug-31 Mo''' <br />
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* '''Sep-01 Tu'''<br />
<br />
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* '''Sep-02 We'''<br />
<br />
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* '''Sep-03 Th'''<br />
<br />
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* '''Sep-04 Fr'''<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
----<br />
<br />
* '''Sep-05 Sa''' - [[Bioenergetics Vienna 2020]]<br />
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* '''Sep-06 So''' <br />
::::* Abstract submission deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
----<br />
2020 Week 37<br />
* '''Sep-07 Mo''' <br />
::::* 19:00 / 20:15 '''MEGA Bildungsshow''' - 1 Million für Österreichs Talente. MARX HALLE Wien, Karl-Farkas-Gasse 19, 1030 Wien. - »[https://www.puls4.com/4GAMECHANGERS 4GAMECHANGERS]«<br />
<br />
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* '''Sep-08 Tu'''<br />
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* '''Sep-09 We'''<br />
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* '''Sep-10 Th'''<br />
<br />
<br />
:::: »<big>'''[[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]]'''</big>« (Sep 10-12)<br />
<br />
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* '''Sep-11 Fr'''<br />
<br />
<br />
2020 Week 38<br />
<br />
* '''Sep-14 Mo''' <br />
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* '''Sep-15 Tu'''<br />
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* '''Sep-16 We'''<br />
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* '''Sep-17 Th'''<br />
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* '''Sep-18 Fr'''<br />
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* '''Sep-19 Sa''' <br />
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* '''Sep-20 So''' <br />
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2020 Week 39<br />
<br />
* '''Sep-21 Mo''' <br />
:::: [[File:Oegmbt.png|100px|link=https://wiki.oroboros.at/index.php/12th_%C3%96GMBT_Annual_Meeting_2020_Graz_AT|ÖGMBT Graz AT]] <big>'''[[12th ÖGMBT Annual Meeting 2020 Graz AT]]'''</big> Sep 21-23<br />
:::: <br />
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* '''Sep-22 Tu''' - [[12th ÖGMBT Annual Meeting 2020 Graz AT]] <br />
<br />
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* '''Sep-23 We''' - [[12th ÖGMBT Annual Meeting 2020 Graz AT]] <br />
<br />
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* '''Sep-24 Th'''<br />
::::* 18:00 - Houska Awards 2020 Vienna - [https://www.bcholding.at/houskapreis-1 Houskapreis der B&C Privatstiftung - Verleihung des Houskapreises 2020 in Wien]<br />
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* '''Sep-25 Fr'''<br />
<br />
2020 Week 40<br />
<br />
* '''Sep-28 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
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* '''Sep-29 Tu''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
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* '''Sep-30 We''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«<br />
::::* Early registration deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
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* '''Oct-01 Th''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
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* '''Oct-02 Fr''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
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* '''Oct-03 Sa''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
2020 Week 41<br />
<br />
* '''Oct-05 Mo''' <br />
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* '''Oct-06 Tu'''<br />
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* '''Oct-07 We'''<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] Pre-Conference O2k-Workshop »<big>'''[[MiPNet25.11 IOC148 Riga LV]] </big>'''«<br />
<br />
::::[[File:EU-CARDIOPROTECTION.png|100px|EU-CARDIOPROTECTION|link=6th EU-Cardioprotection Meeting 2020 Riga LV]] »<big>'''[[6th EU-Cardioprotection Meeting 2020 Riga LV]]'''</big>« (Oct 7-9)<br />
<br />
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* '''Oct-08 Th''' - [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
<br />
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* '''Oct-09 Fr''' - [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
:::: »<big>'''[[Long Night of Research 2020 Innsbruck AT]]'''</big>«<br />
<br />
2020 Week 42<br />
<br />
* '''Oct-12 Mo''' <br />
::::[[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Riga LV]]'''</big>« (Oct 7-9)<br />
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* '''Oct-13 Tu'''<br />
::::[[FAT4BRAIN School IOC147 Riga LV]]<br />
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* '''Oct-14 We'''<br />
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* '''Oct-15 Th'''<br />
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* '''Oct-16 Fr'''<br />
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* '''Oct-18 Su'''<br />
:::: »<big>'''[[ISCaM 2020 London UK]]'''</big>«<br />
<br />
2020 Week 43<br />
<br />
* '''Oct-19 Mo''' - [[ISCaM 2020 London UK]]<br />
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* '''Oct-20 Tu''' - [[ISCaM 2020 London UK]]<br />
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* '''Oct-21 We'''<br />
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* '''Oct-22 Th'''<br />
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* '''Oct-23 Fr'''<br />
<br />
2020 Week 44<br />
<br />
* '''Oct-26 Mo''' <br />
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* '''Oct-27 Tu'''<br />
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* '''Oct-28 We'''<br />
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* '''Oct-29 Th'''<br />
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* '''Oct-30 Fr'''<br />
<br />
2020 Week 45<br />
<br />
* '''Nov-02 Mo''' <br />
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* '''Nov-03 Tu'''<br />
<br />
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* '''Nov-04 We'''<br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] <big>'''[[MitoEAGLE Summit Obergurgl 2020]]'''</big><br />
----<br />
* '''Nov-05 Th''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
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* '''Nov-06 Fr''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
* '''Nov-07 Sa''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
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2020 Week 46<br />
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* '''Nov-09 Mo''' <br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] <big>'''[[MiPschool Obergurgl 2020]]'''</big> including '''[[TRANSMIT School 2020]]'''<br />
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* '''Nov-10 Tu''' - [[MiPschool Obergurgl 2020]] including [[TRANSMIT School 2020]]<br />
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* '''Nov-11 We''' - [[MiPschool Obergurgl 2020]] including [[TRANSMIT School 2020]]<br />
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* '''Nov-12 Th'''<br />
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* '''Nov-13 Fr'''<br />
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2020 Week 47<br />
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* '''Nov-16 Mo''' <br />
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* '''Nov-17 Tu'''<br />
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* '''Nov-18 We''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
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* '''Nov-19 Th''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
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* '''Nov-20 Fr''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
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2020 Week 48<br />
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* '''Nov-23 Mo''' <br />
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* '''Nov-24 Tu'''<br />
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* '''Nov-25 We'''<br />
::::» <big>'''[[2020Spring PaduaMuscleDays Padova IT]]'''</big> / [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.04 IOC Padua IT |<big>'''IOC Padua IT'''</big>]] - ''separate registration''<br />
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* '''Nov-26 Th''' <br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
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* '''Nov-27 Fr'''<br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
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* '''Nov-28 Sa'''<br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
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2020 Week 49<br />
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* '''Nov-30 Mo''' <br />
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* '''Dec-01 Tu'''<br />
:::: [[Image:AlgaeEurope.jpg|60px|AlgaeEurope]] <big>'''[[AlgaEurope 2020 Rome IT]]'''</big><br />
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* '''Dec-02 We'''<br />
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* '''Dec-03 Th'''<br />
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* '''Dec-04 Fr'''<br />
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* '''Dec-05 Sa'''<br />
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* '''Dec-06 So'''<br />
::::* Registration deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
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2020 Week 50<br />
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* '''Dec-07 Mo''' <br />
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* '''Dec-08 Tu'''<br />
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* '''Dec-09 We'''<br />
::::[[File:ESSR2020.png|100px|ESSR2020|link=https://wiki.oroboros.at/index.php/ESSR_2020_Innsbruck_AT]] <big>'''[[ESSR 2020 Innsbruck AT]]'''</big> (Dec 9-11)<br />
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* '''Dec-10 Th'''<br />
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* '''Dec-11 Fr'''<br />
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2020 Week 51<br />
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* '''Dec-14 Mo''' <br />
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* '''Dec-15 Tu'''<br />
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* '''Dec-16 We'''<br />
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* '''Dec-17 Th'''<br />
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* '''Dec-18 Fr'''<br />
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2020 Week 52<br />
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* '''Dec-21 Mo''' <br />
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* '''Dec-22 Tu'''<br />
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* '''Dec-23 We'''<br />
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* '''Dec-24 Th'''<br />
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* '''Dec-25 Fr'''<br />
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2020 Week 53<br />
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* '''Dec-28 Mo''' <br />
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* '''Dec-29 Tu'''<br />
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* '''Dec-30 We'''<br />
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* '''Dec-31 Th'''<br />
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* '''Jan-01 Fr'''</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=205923
Oroboros Ecosystem Agenda 2020 Archive
2020-08-10T06:08:28Z
<p>Antunes Diana: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
[[File:Oroboros_EcosystemAgenda_2020.png|right|560px|Oroboros Ecosystem Agenda 2020]]<br />
{{Template:OROBOROS Slogan}}<br />
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::::<big>» [[Oroboros Ecosystem Agenda 2020 |Current Oroboros Ecosystem Agenda 2020]]</big><br />
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__TOC__<br />
2020 Week 32<br />
* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br><br />
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]«<br />
|}<br />
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* '''Aug-04 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br><br />
:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]« <br />
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]<br />
|}<br />
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* '''Aug-05 We'''<br />
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* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|} <br />
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* '''Aug-07 Fr'''<br />
2020 Week 31<br />
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* '''Jul-27 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''<br />
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br><br />
- »[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«<br />
|} <br />
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* '''Jul-28 Tu''' - World Hepatitis Day<br />
:{|<br />
<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Insights into Hepatitis C virus-linked mitochondrial dysfunction''' <br><br />
: from the O2k-Network »[[IT Foggia Capitanio N]]« <br><br />
* Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60. [[Ripoli 2009 J Virol|»Bioblast link«]]<br />
|}<br />
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* '''Jul-29 We''' <br />
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* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
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* '''Jul-31 Fr'''<br />
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2020 Week 30<br />
<br />
* '''Jul-20 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''How can you compare respirometric experiments using an internal normalization?'''<br><br />
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”. <br />
<br><br />
- »[[Flux control ratio]]«<br />
<br />
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
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* '''Jul-21 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br><br />
:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]« <br />
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. [[Lantier 2020 Diabetes|»Bioblast link«]]<br />
|}<br />
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* '''Jul-22 We''' <br />
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* '''Jul-23 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]<br />
| '''First [[O2k-Webinars|O2k-Webinar]] - O2k-Applications: H<sub>2</sub>O<sub>2</sub> production by [[Komlodi Timea|Dr. Tímea Komlódi]] ''' <br />
|}<br />
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* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
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2020 Week 29<br />
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* '''Jul-13 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br><br />
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. <br />
For more information, see:<br />
- »[[Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP|Copy from file]]«<br />
|}<br />
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* '''Jul-14 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br><br />
<br />
:from the O2k-Network »[[BR Ribeirao Preto Alberici LC]]« <br />
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|»Bioblast link«]]<br />
|}<br />
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* '''Jul-15 We''' <br />
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* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
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* '''Jul-17 Fr'''<br />
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2020 Week 28 <br />
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* '''Jul-06 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br><br />
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear.<br />
For more information, see:<br><br />
- »[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]« and »[[DatLab error messages]] «<br />
|}<br />
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* '''Jul-07 Tu''' <br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br><br />
:from the O2k-Network »[[FR Lille Duez H]]« <br />
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|»Bioblast link«]]<br />
|}<br />
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* '''Jul-08 We''' <br />
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* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
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* '''Jul-10 Fr'''<br />
<br />
== Jun 2020 ==<br />
2020 Week 27<br />
<br />
* '''Jun-29 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br><br />
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br><br />
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«<br />
|}<br />
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* '''Jun-30 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]« <br />
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]<br />
|}<br />
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* '''Jul-01 We''' <br />
:{|<br />
| width="100" align="center" |[[Image:TRANSMIT Acronym.jpg|70px|link=https://wiki.oroboros.at/index.php/TRANSMIT#TRANSMIT_dissemination |TRANSMIT]]<br />
|'''Kick-off of the TRANSMIT crowdfunding campaign: NOW'''<br><br />
:Bring the world of scientific research closer to the world of patients by supporting four cancer patients’ associations.<br />
<br />
:- »[https://www.ideaginger.it/progetti/now.html https://www.ideaginger.it/progetti/now.html]«<br />
|}<br />
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* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
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* '''Jul-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br><br />
: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br><br />
* Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]<br />
|}<br />
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2020 Week 26<br />
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* '''Jun-22 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Did you know how a stirrer test can save your experiments?'''<br><br />
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
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* '''Jun-23 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br><br />
:from the O2k-Network »[[IT Verona Calabria E]]« <br />
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|»Bioblast link«]]<br />
|}<br />
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* '''Jun-24 We'''<br />
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* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
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* '''Jun-26 Fr'''<br />
<br />
{|<br />
| width="110" align="center" | <br />
<br />
[[Image:MitoGlobal.jpg|80px|left|MitoGlobal]]<br />
<br />
| Join and disseminate the [https://mitowomen.org/index.html '''“Women in Mitochondria”'''] initiative which aims to make the women working in the mitochondrial field more visible, contributing to accelerating equality and diversity. <br><br />
|}<br />
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2020 Week 25<br />
<br />
* '''Jun-15 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
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* '''Jun-16 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br><br />
:from the O2k-Network »[[CA Montreal Hepple RT]]« <br />
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|»Bioblast link«]]<br />
|}<br />
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* '''Jun-17 We''' <br />
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* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
<br />
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* '''Jun-19 Fr'''<br />
<br />
2020 Week 24<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
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* '''Jun-09 Tu''' <br />
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* '''Jun-10 We'''<br />
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* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
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* '''Jun-15 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|''' Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
----<br />
* '''Jun-12 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ<sub>10</sub> in the platelets of CKD patients''' <br><br />
: from the O2k-Network » [[ SK Bratislava Sumbalova Z]] « <br><br />
* Gvozdjáková A, Sumbalová Z, Kucharská J, Komlósi M, Rausová Z, Vančová O, Számošová M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q<sub>10</sub> and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|»Bioblast link«]]<br />
<br />
|}<br />
<br />
<br />
2020 Week 23 <br />
<br />
* '''Jun-01 Mo''' <br />
:{|<br />
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«<br />
|}<br />
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* '''Jun-02 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br><br />
:from the O2k-Network »[[AT Innsbruck Gnaiger E]]« , »[[CA Rimouski Blier PU]]« and »[[CA Edmonton Lemieux H]]« <br />
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|»Bioblast link«]]<br />
|}<br />
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* '''Jun-03 We''' <br />
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* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Mitochondria show variable preferences for [[GM|glutamate & malate]] or [[PM|pyruvate & malate]] to support NADH-linked respiration, depending on species, tissues, and pathophysiological state.''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to compare respiratory capacities supported by these NADH-linked fuel substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
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* '''Jun-05 Fr'''<br />
<br />
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== May 2020 ==<br />
2020 Week 22 <br />
<br />
* '''May-25 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br><br />
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br><br />
- »[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«<br />
|}<br />
----<br />
* '''May-26 Tu''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br><br />
: from the O2k-Network » [[ AT Innsbruck Gnaiger E]] « and » [[ AT Innsbruck Oroboros]] « <br><br />
* Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. [[Schoepf 2020 Nat Commun|»Bioblast link«]]<br />
<br />
|}<br />
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* '''May-27 We''' <br />
:{| <br />
| width="100" align="center" |[[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]<br />
|The decline of mitochondrial respiratory function is strongly associated with obesity. This may play a potential role in determining corona virus severity. Read more: https://wiki.oroboros.at/index.php/Viruses_and_mitochondrial_medicine<br />
<br />
To fight COVID-19 the world urgently needs effective and safe diagnostics, treatments and vaccines. The EU is organizing a Global Response pledging effort to finance life-saving solutions. Join the marathon and spread the word: https://europa.eu/global-response/#UnitedAgainstCoronavirus<br />
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|}<br />
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* '''May-28 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
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* '''May-29 Fr'''<br />
<br />
:{|<br />
<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br><br />
:from the O2k-Network »[[BR Sao Paulo Silber AM]]« <br />
*Alberto Luévano-Martínez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. [[Alberto Luevano-Martinez 2020 FEBS Lett|»Bioblast link«]]<br />
|}<br />
<br />
2020 Week 21 <br />
<br />
* '''May-18 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br><br />
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br><br />
- »[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«<br />
|}<br />
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* '''May-19 Tu''' <br />
:{|<br />
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''«<br><br />
:from the O2k-Network »[[Charles Anne-Laure]]« »[[Geny Bernard]]«<br />
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|»Bioblast link«]]<br />
|}<br />
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* '''May-20 We''' <br />
:{|<br />
| width="100" align="center"|[[Image:SI-units.png|65px|link=https://wiki.oroboros.at/index.php/BEC2020.1_doi10.26124bec2020-0001.v1#SI_units|SI Units]]<br />
|'''SI units'''<br><br />
One year ago, on [https://www.euramet.org/publications-media-centre/news/?tx_news_pi1%5Bnews%5D=787&tx_news_pi1%5Baction%5D=detail&tx_news_pi1%5Bcontroller%5D=News World Metrology Day] (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of '''Mitochondrial physiology''' and with the launch of an innovative journal — '''[[Bioenergetics Communications]]'''.<br />
|}<br />
<br />
:{|<br />
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]<br />
|MitoEAGLE ''Consortium Communication'': '''Mitochondrial physiology''' ― Published online: 2020-05-20.<br />
Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1<br><br />
» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1<br />
|}<br />
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* '''May-21 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
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* '''May-22 Fr'''<br />
<br />
2020 Week 20<br />
<br />
* '''May-11 Mo'''<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.'''<br><br />
Learn more about steady states including performance and mark setting for data analysis <br><br />
- »[[Steady state]]«<br />
|}<br />
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* '''May-12 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Mitochondria - a driving force in adipocyte differentiation''' <br><br />
:from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br><br />
* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]<br />
|}<br />
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* '''May-13 We'''<br />
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* '''May-14 Th'''<br />
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:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
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* '''May-15 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br><br />
: from the O2k-Network »[[ FI Helsinki Jacobs HT]]« , »[[ DE Magdeburg Gellerich FN]]« , »[[FI Tampere Dufour E]]« , »[[DE Magdeburg Debska-Vielhaber G]]« and »[[DE Frankfurt Wittig I]]«<br><br />
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet Ma, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT (2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]<br />
|}<br />
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* '''May-17 Su''' World Hypertension Day <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br><br />
:from the O2k-Network »[[CZ Prague Houstek J]]« and »[[CZ Prague Kalous M]]«<br />
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|»Bioblast link«]]<br />
|}<br />
2020 Week 19<br />
<br />
* '''May-04 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br><br />
Perform and apply the instrumental background test:<br><br />
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«<br />
|}<br />
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* '''May-05 Tu''' <br />
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''<br />
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]« »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«<br />
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]<br />
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* '''May-06 We''' <br />
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* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: »'''[[Malate-anaplerotic pathway control state]]'''«<br />
<br />
- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br />
<br />
- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
<br /><br />
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* '''May-08 Fr'''<br />
<br />
== Apr 2020 ==<br />
<br />
2020 Week 18<br />
<br />
* '''Apr-27 Mo''' <br />
:::: [[Image:O2k-Support.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''<br />
::::::: The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub> concentrations. - »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«<br />
<br />
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* '''Apr-28 Tu''' <br />
::::[[Image:O2k-Publications.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake'''<br />
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«, »[[DE Magdeburg Gellerich FN]]« and »[[DE Magdeburg Debska-Vielhaber G]]« <br />
::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - [[Szibor 2020 J Biol Chem|»Bioblast link«]]<br />
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<br />
* '''Apr-29 We''' <br />
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* '''Apr-30 Th'''<br />
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* '''May-01 Fr'''<br />
<br />
2020 Week 17<br />
* '''Apr-20 Mo''' <br />
<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''<br />
<br />
::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«<br />
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]<br />
<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''<br />
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«<br />
<br />
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<br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.<br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
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* '''Apr-22 We''' <br />
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* '''Apr-23 Th''' <br />
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* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]].<br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
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2020 Week 16<br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''<br />
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of [[mitochondrial preparations]] - »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
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* '''Apr-14 Tu''' <br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''<br />
::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«<br />
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* '''Apr-15 We''' <br />
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* '''Apr-16 Th'''<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease'''<br />
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::::::: - from the O2k-Network »[[DE Frankfurt Eckert GP]]« »[[CH Basel Eckert A]]«<br />
::::::* Leuner K, Schulz K, Schütt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, Müller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204. - [[Leuner 2012 Mol Neurobiol|»Bioblast link«]] <br />
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* '''Apr-17 Fr'''<br />
2020 Week 15<br />
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* '''Apr-06 Mo''' <br />
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<big><big>{{Template:Humankind COVID-19}}</big></big><br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Physical activity has positive effects on mitochondria from patients with occupational burnout''' <br />
::::::: - from the O2k-Network »[[CH Basel Eckert A]]«<br />
::::::* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|»Bioblast link«]]<br />
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* '''Apr-07 Tu''' <br />
:::: [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[File:Blue-info.png|40px|Info: improve]] '''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''''<br />
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::::::: If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. - »[[SUIT: Browse DL-Protocols and templates]]«<br />
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* '''Apr-08 We''' <br />
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''<br />
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - »'''[[SUIT-010 |SUIT-010]]'''«<br />
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* '''Apr-09 Th'''<br />
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* '''Apr-10 Fr'''<br />
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2020 Week 14<br />
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* '''Mar-30 Mo'''<br />
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:''' »'''[[Enable DL-Protocol editing]]'''«<br />
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* '''Mar-31 Tu''' <br />
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* '''Apr-01 We''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria''' <br />
::::::: - from the O2k-Network »[[FR Angers Gueguen N]]« and »[[BE Leuven Spinazzi M]]«<br />
::::::* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|»Bioblast link«]]<br />
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[[File:Tony hickey garage.jpg|200px|thumb|Non-stoppable: Tony Hickey in home-office mode on high-resolution respirometry]]<br />
* '''Apr-02 Th''' <br />
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?''' Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
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::::[[Image:O2k-Network.png|70px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] Creative solutions coming from the O2k-Network labs - » [[NZ Auckland Hickey AJ]] <br />
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* '''Apr-03 Fr'''<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''<br />
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::::::: - from the O2k-Network »[[DE Nuthetal Klaus S]]«<br />
::::::* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - [[Ost 2020 EMBO Rep |»Bioblast link«]] <br />
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== Mar 2020 ==<br />
2020 Week 13<br />
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* '''Mar-23 Mo''' <br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''<br />
::::::: '''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br><br />
::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''«<br />
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* '''Mar-24 Tu'''<br />
:::: [[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Tuberculosis Day: '''Mitochondria as target to ''Mycobacterium tuberculosis'' infection''' <br />
::::::: - from the O2k-Network »[[US NC Durham Li PA]]«<br />
::::::* Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - [[Lu 2018 ACS Infect Dis |»Bioblast link«]]<br />
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* '''Mar-25 We''' <br />
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] Preprint: '''An urgent call for research on COVID-19 and public vitamin D awareness'''<br />
::::::* Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. MitoFit Preprint Arch [[doi:10.26124/mitofit:200001]].<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Research findings made available via preprint servers before journal publication''' - [https://wellcome.ac.uk/press-release/sharing-research-data-and-findings-relevant-novel-coronavirus-covid-19-outbreak?sap-outbound-id=CF563AB7AA7FEDFB8731215C98A59C11790C0456&utm_source=hybris-campaign&utm_medium=email&utm_campaign=101_PBN2619_0000020211_GLS_101_aut_202003_Coronavirus_Forschung&utm_content=EN_internal_44988_20200325&mkt-key=005056A5C6311EE9999982134B93151D Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak]<br />
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]]'''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4: »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br />
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* '''Mar-26 Th'''<br />
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* '''Mar-27 Fr'''<br />
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2020 Week 12 <br />
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* '''Mar-16 Mo''' <br />
::::: [[Image:Logo_OROBOROS_INSTRUMENTS.jpg|left|60px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]] '''Oroboros operates from now on in home office mode - we stay connected.'''<br />
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:::: [[Image:SUIT icon.jpg|80px|left|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br />
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »'''[[SUIT-003 |SUIT-003]]'''«<br />
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* '''Mar-17 Tu'''<br />
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::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ''' Zika virus infection leads to mitochondrial dysfunction''' <br />
::::::: - from the O2k-Network »[[BR Rio de Janeiro Galina A]]«<br />
::::::* Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - [[Ledur 2020 Sci Rep |»Bioblast link«]]<br />
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* '''Mar-18 We''' <br />
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:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Exercise protects against the effects of aging on mitochondrial function'''<br />
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::::::: - from the O2k-Network »[[KR Busan Han J]]« »[[KR Incheon Kwak HB]]«<br />
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]] <br />
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* '''Mar-19 Th''' <br />
:::: [[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis '''Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home'''.<br />
::::::: - » For the download link and password, please email [mailto:instruments@oroboros.at instruments@oroboros.at]<br />
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* '''Mar-20 Fr'''<br />
::::* '''Abstract submission deadline''' - »[[SHVM 2020 Halifax CA]]«<br />
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2020 Week 11<br />
* '''Mar-08 Su''' - '''[https://www.internationalwomensday.com/ International Women's Day]''' <br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Addressing the underrepresentation of women |»Bioblast link«]]<br />
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* '''Mar-09 Mo''' <br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] »<big>'''[[MitoEAGLE WG2 Innsbruck AT]]'''</big>« (Mar 09-13)<br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Protein-specific mitochondrial respiratory capacity is higher in women than men''' <br />
::::::: - from the O2k-Network »[[SE Stockholm Cardinale DA]]«<br />
::::::* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |»Bioblast link«]]<br />
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* '''Mar-10 Tu'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Muscular exercise for training adipose tissue mitochondria''' <br />
::::::: - from the O2k-Network »[[DK Copenhagen Larsen S]]«<br />
::::::* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |»Bioblast link«]]<br />
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* '''Mar-11 We'''<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. - »'''[[Blebbistatin]]'''«<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Let's talk COVID-19 - a new culture of communication in science.''' - [https://www.sciencemag.org/news/2020/02/completely-new-culture-doing-research-coronavirus-outbreak-changes-how-scientists Kay Kupferschmidt in Science]<br />
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::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while operating the O2k with biological sample?'''<br />
::::::: - It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before [[uncoupler titrations]]. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«<br />
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* '''Mar-12 Th'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis''' <br />
::::::: - from the O2k-Network »[[UK London Duchen MR]]«<br />
::::::* Warne J, Pryce G, Hill J, Shi X, Lennerås F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |»Bioblast link«]] <br />
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* '''Mar-13 Fr'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Men and women with Type 1 diabetes and skeletal muscle mitochondria''' <br />
::::::: - from the O2k-Network »[[CA Toronto Perry CG]]«<br />
::::::* Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - [[Monaco 2020 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
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2020 Week 10<br />
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* '''Mar-02 Mo''' <br />
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] An amazing number of new [[O2k-Publications: Topics |O2k-Publications]] from China. - »'''[[O2k-Network Labs China]]'''«<br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction''' <br />
::::::: - from the O2k-Network »[[CN Chongqing Zhu Z]]«<br />
::::::* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |»Bioblast link«]] <br />
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* '''Mar-03 Tu''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Rapamycin suppresses senescence in galactose versus glucose cell culture''' <br />
::::::: - from the O2k-Network »[[US PA Philadelphia Orynbayeva Z]]«<br />
::::::* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |»Bioblast link«]]<br />
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:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? - »'''[[Rapamycin]]'''«<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Advancing Open Science practices: stakeholder perspectives on incentives and disincentives.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Advancing Open Science practices |»Bioblast link«]] - The concept of [[Gnaiger 2019 MitoFit Preprint Arch Editorial |Preprints]] needs full attention in Open Science.<br />
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* '''Mar-04 We'''<br />
[[File:Platelet.jpg|right|200px]]<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets''' <br />
::::::: - from the O2k-Network »[[US FL Orlando Goodpaster BH]]«<br />
::::::* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |»Bioblast link«]] <br />
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[[File:Softdrink.jpg|right|100px]]<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Sugar and fat - initial mitochondrial aging in the brain'''<br />
::::::: - from the O2k-Network »[[IT Naples Iossa S]]«<br />
::::::* Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - [[Crescenzo 2019 Mol Neurobiol |»Bioblast link«]] <br />
[[File:OroboPOS-Holder002.jpg|right|100px]]<br />
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''<br />
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«<br />
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* '''Mar-05 Th'''<br />
::::* '''Abstract submission opens''' - »[[Europhysiology 2020 Berlin DE]]«<br />
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:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] With a '''preprint''' you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - »'''[[Preprint]]'''«<br />
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* '''Mar-06 Fr'''<br />
:::: [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''Explore metabolic profiles and specific modultations of mitochondrial pathways''' in health and disease with SUIT reference protocols. - »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
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== Feb 2020 ==<br />
<br />
2020 Week 9<br />
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* '''Feb-23 Su'''<br />
:::::::» '''[[2020 PaduaMuscleDays Padova IT]]''' - ''postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy''<br />
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* '''Feb-24 Mo''' <br />
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] More research is needed on the impact of viral infection on mitochondrial function. - »'''[[Viruses and mitochondrial medicine]]'''«<br />
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* '''Feb-25 Tu'''<br />
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] High quality mitochondrial research is increasing rapidly in South Korea. - »'''[[O2k-Network Labs Republic of Korea]]'''«<br />
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<br /><br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''An active lifestyle protects cardiac mitochondria''' <br />
::::::: - from the O2k-Network: [[KR Busan Han J]], [[KR Incheon Kwak HB]]<br />
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]] <br />
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[[File:2020-02-26 MitoEAGLE WG4 Innsbruck AT.jpg|right|400px|thumb|MitoEAGLE WG4 retreat; left to right: [[Garcia-Souza Luiz F |Luiz]], [[Gnaiger Erich |Erich]], [[Aasander Frostner Eleonor |Eleonor]], [[Michalak Slawomir |Slawomir]]]]<br />
* '''Feb-26 We''' <br />
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. - »'''[[Noncoupled respiration]]'''«<br />
<br /><br />
<br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] '''[[MitoEAGLE WG4 Innsbruck AT]]''' (Feb 26-28)<br />
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* '''Feb-27 Th'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase''' <br />
::::::: - from the O2k-Network: [[CZ Prague Houstek J]]<br />
::::::* Pajuelo-Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |»Bioblast link«]] <br />
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* '''Feb-28 Fr'''<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''<br />
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«<br />
<br />
<br />
2020 Week 8<br />
<br />
* '''Feb-17 Mo''' <br />
:::: [[File:EBEClogo2020.jpg|80px|link=https://www.mitoglobal.org/index.php/EBEC2020_Marseille_FR]] '''Registration is open: [[EBEC2020_Marseille_FR |European Bioenergetics Conference 2020]]''', Marseille, France, 2020<br />
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* '''Feb-18 Tu''' <br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? » Dive deep into '''[[Oxygen solubility]]'''<br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis''' <br />
::::::: - from the O2k-Network »[[US MI Ann Arbor Beard DA]]«<br />
::::::* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. - [[Tyrrell 2019 Circ Res |»Bioblast link«]]<br />
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* '''Feb-19 We''' <br />
:::: [[Image:MitoFit Preprint Arch.png|80px|link=MitoFit Preprint Arch]] - »'''[[MitoFit Preprint Arch]]'''« was initiated one year ago. We invite submissions of mitochondrial preprints.<br />
<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »'''[[Spermidine]]'''«<br />
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* '''Feb-20 Th'''<br />
:::: [[Image:Logo MitoFit s.jpg|40px|link=MitoFit Open Seminars]] - '''[[MitoFit Open Seminars|MitoFit Open Seminar]]''': [[Stefan_E|Eduard Stefan]]: “Decoding and Perturbation of ONCO-Kinase functions” and [[Torres-Quesada_O|Omar Torres Quesada]]: “'''[[MitoKIN]]''' - Systematic barcoding of kinase-controlled mitochondrial function”<br />
<br />
<br />
2020 Week 7<br />
<br />
*'''Feb-10 Mo''' <br />
::::* '''Early Bird Registration Deadline''' for [[ESSR 2020 Innsbruck AT]] <br />
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*'''Feb-12 We''' <br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »'''[[MitObesity drugs]]'''«<br />
<br />
<br />
:::: [[Image:O2k-Feedback.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] ''I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner.'' - '''[[Gayathri Narayanappa]]''' participaing in [[MiPNet25.03 IOC144 Innsbruck AT]]<br />
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*'''Feb-13 Th'''<br />
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<br />
2020 Week 6<br />
<br />
*'''Feb-03 Mo''' <br />
:::[[File:FFG-logo.png|150px|FFG]] '''<big>»Official start of the FFG funded project »[[MitoKIN|'''MitoKIN''']]«</big>''' <br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' <br />
::::::: - from the O2k-Network »[[US AR Little Rock Boersheim E]]« <br />
::::::* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
<br />
::::* Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, [https://wiki.oroboros.at/index.php/Tremblay_N Dr Nelly Tremblay] is using for the first time the [[SUIT-022]] protocol to study alternative oxidases in the marine model organism ''Phaeodactylum tricornutum''.<br />
<br />
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*'''Feb-04 Tu''' <br />
:::: [[Image:NextGenO2k logo.jpg|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.10 IOC and Distributor engagement Tokyo JP |'''MiPNet25.10 IOC and Distributor engagement Tokyo JP''']] (Feb 4-7)<br />
:::: [[File:O2k-brief.png|50px|link=https://wiki.oroboros.at/images/7/7b/Cronin_2018_Nature_O2k-brief.pdf|O2k-brief]] '''Cronin''' (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - '''Lymphocytes''', '''Cancer''' - [http://wiki.oroboros.at/index.php/O2k-brief O2k-brief repository]<br />
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*'''Feb-05 We'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease'''<br />
::::::: - from the O2k-Network »[[US FL Boca Raton Khamoui AV]]«<br />
::::::* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |»Bioblast link«]]<br />
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*'''Feb-06 Th'''<br />
:::: '''MitoFit Open Seminar'''<br />
:::::* 16:00-16:30 [[Beleza J|Jorge Beleza]]: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. [[Media:MitoFitOpenSeminar_JorgeBeleza_20200206.pdf|»Abstract«]]<br />
:::::* 16:30-17:00 [[Pereira R|Rita Pereira]]: The Handball4Health Project : A community-based intervention programme<br />
<br />
<br />
== Jan 2020 ==<br />
<br />
2020 Week 5<br />
<br />
*'''Jan-27 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.06 IOC145 Innsbruck AT |<big>'''IOC145 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 27-29)<br />
<br />
:::: DSL-Seminar, 16:00<br />
:::::: [[Gnaiger E]]: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.<br />
:::::: Univ.-Klinik für Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room<br />
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*'''Jan-28 Tu'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration of blood cells and cardiovascular disease''' <br />
::::::: - from the O2k-Network »[[FR Strasbourg Zoll J]]« <br />
::::::* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - [[Alfatni 2020 J Clin Med |»Bioblast link«]]<br />
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*'''Jan-30 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Karabatsiakis_A|Alexander Karabatsiakis]]: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''<br />
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »[[Oxygen calibration - DatLab]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?'''<br />
<br />
::::::: Your air calibration is not effective until you set mark ‘R1’ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - »[[Oxygen calibration - DatLab]]« <br />
<br />
<br />
::::* '''Open Innovation''' in the NextGen-O2k software development DatLab 8. Here is a summary of [[MitoPedia: DatLab |MitoPedia]] pages including [[:Category:DatLab 8 perspectives |'''DatLab 8 perspectives''']]<br />
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*'''Jan-31 Fr'''<br />
::::* Deadline for [[APS2020_Chicago_US|APS2020]] Abstract submission for poster presentation.'''<br />
::::* [[2020 PaduaMuscleDays Padova IT |Call for EJTM Communications]] - deadline for manuscript submissions<br />
::::* Registration deadline for [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''<br />
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »[[Oxygen calibration - DatLab]]«<br />
<br />
2020 Week 4<br />
<br />
*'''Jan-20 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.03 IOC144 Innsbruck AT |<big>'''IOC144 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 20-22)<br />
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*'''Jan-21 Tu'''<br />
::::* Mr Hai Wu (CEO of [[CN Beijing Huawei |Huawei]], Oroboros distributor in China) and Dr. Rupert Ecker (CEO of [https://www.tissuegnostics.com/en/TissueGnostics Vienna TissueGnostics]) join the O2k-Coaching Days for extending collaborative projects.<br />
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*'''Jan-23 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Gnaiger E|Erich Gnaiger]]: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.<br />
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*'''Jan-24 Fr'''<br />
::::* Further circulars to invited speakers for submission of tentative titles for oral and poster presentations - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''<br />
::::::: Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - »[[O2k configuration]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''<br />
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?<br />
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]«<br />
<br />
2020 Week 3<br />
<br />
*'''Jan-14 Tu''' <br />
::::* Circular ITC Grants for [[2020 PaduaMuscleDays Padova IT]], [[6th EU-Cardioprotection Meeting 2020 Riga LV]] and [[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]] - [https://wiki.oroboros.at/images/a/af/ITC_Newsletter.pdf pdf]<br />
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*'''Jan-15 We''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers''' <br />
::::::: - from the O2k-Network »[[AU Melbourne Stepto NK]]« <br />
::::::* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - [[Jacques 2020 FASEB J |»Bioblast link«]]<br />
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*'''Jan-16 Th'''<br />
::::[[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Oxygen flux]]''' - which SI units should be used?<br />
<br />
::::[[File:MitoPedia.jpg|60px|MitoPedia]] Did you ever think that units for body mass could be an issue? - »'''[[Body mass]]'''«<br />
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*'''Jan-17 Fr''' <br />
::::[[Image:O2k-close.jpg|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]] <big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. - [[Scandurra_2010_Adv_Exp_Med_Biol |»Bioblast link«]]<br />
<br />
:::: [[Image:TRANSMIT Acronym.jpg|60px|TRANSMIT]] <big>'''[[TRANSMIT Symposium 2020 Brussels BE]]'''</big> (Jan 17-18)<br />
<br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations''' <br />
::::::: - from the O2k-Network »[[CZ Prague Krajcova A]]« <br />
::::::* Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |»Bioblast link«]]<br />
<br />
2020 Week 2<br />
<br />
*'''Jan-07 Tu''' The Oroboros Office opens in the New Year 2020<br />
[[File:EBEClogo2022.jpg|right|300px|right|link=EBEC22 Innsbruck AT]]<br />
::::* From the '''Austrian Bioenergetics Group''' to '''Mitochondrial Physiology and Bioenergetics DACH''' (D: Germany; A: Austria; CH: Switzerland) <br />
:::::: [[EBEC22 Innsbruck AT |EBEC22 - the 22nd European Bioenergetics Conference]] will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the ''EBEC22 National Scientific Committee''. This provides an excellent opportunity to establish an ''Austrian Bioenergetics Group'' towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming [[1st Myocardial Function Symposium 2020 Graz AT]], and further at the [[MitoEAGLE Summit Obergurgl 2020]]. Please, contact the organizer of EBEC22 - erich.gnaiger@oroboros.at<br />
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* '''Jan-08 We'''<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
<br />
:::: [[Image:Quality.png|left|60px|link=http://wiki.oroboros.at/index.php/MitoPedia:_Oroboros_QM |Quality]] <br />
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* '''Jan-09 Th'''<br />
[[File:MITOEAGLE-representation.jpg|right|200px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]]<br />
::::* Planning the [[MitoEAGLE Summit Obergurgl 2020]]<br />
:::::: Members of the [[Management Committee MitoEAGLE]] and [[MitoEAGLE Working Groups |Working Group Leaders]]: please submit your suggestions for [[MitoEAGLE Summit Obergurgl 2020]]:<br />
::::::# Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output<br />
::::::# Suggested scientists (titles) for presentations, according to the MoU<br />
<br />
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* '''Jan-10 Fr''' <br />
::::» <big>'''[[1st Myocardial Function Symposium 2020 Graz AT]]'''</big> (Jan 10-11)<br />
::::* 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)<br />
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* '''Jan-12 So'''<br />
::::* One month ago<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:: '''Cluster Awards 2019'''<br />
<br />
<hr><br />
<br />
{|<br />
|[[File:Cluster Awards 2019.jpg|right|500px|Cluster Awards 2019]]<br />
On December 12<sup>th</sup>, the '''Cluster Awards 2019''' were presented for the best Tyrolean innovations of the year. We are thrilled to be the recipient in the '''Life Sciences Cluster''' for our EU Horizon 2020-funded '''[[NextGen-O2k |NextGen-O2k project]]'''.<br />
<br />
'''Life Sciences'''<br />
::: Oroboros Instruments GmbH, Innsbruck: "NextGen-O2k"<br />
<br />
'''Renewable Energies'''<br />
::: Heliotherm Wärmepumpentechnik GmbH, Langkampfen: "Invisible Heatpump"<br />
<br />
'''Forest and wood'''<br />
::: Holzbau Unterrainer GmbH, Ainet: "Radius wood - production of curved cross laminated timber elements"<br />
<br />
'''Information Technologies'''<br />
::: Lo.La Peak Solutions GmbH, Trins: "LO.LA Safety - Digital Safety Management in Ski Resorts"<br />
<br />
'''Mechatronics''': ALPEX Technologies GmbH, Mils: "EVEREST 4.0"<br />
<br />
'''Wellness''': Organoid Technologies GmbH, Flow: "natural sauna surfaces made of hay"<br />
<br />
» More details (in German): '''[https://www.standort-tirol.at/page.cfm?vpath=newsroom/news&genericpageid=26328 Standortagentur Tirol]'''.<br />
<div style="text-align: right; direction: ltr; margin-left: 1em;">Photo © Standortagentur Tirol</div><br />
|}<br />
|}<br />
<br />
2020 Week 1 '''The Oroboros team enjoys ''holidays'' from 2019 Dec 24 to 2020 Jan 06'''<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: #white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:'''[[Oroboros picture of the month]]'''<br />
<br />
<hr><br />
{|<br />
| <br />
[[File:Oroboros Winter.jpg|250px|Oroboros of the month]]<br />
| ''' Season's Greetings'''<br />
<br />
: Our office is closed until 2020-01-06. <br />
<br />
: We wish you a Happy New Year 2020! <br />
<br />
: Your Oroboros team<br />
::::::* the [[Oroboros_contact#Oroboros_Customer_Relations_Ninjas |Oroboros Customer Relations Ninjas]]<br />
::::::* the [[Oroboros_contact#Oroboros_Sales_Athletes |Oroboros Sales Athletes]]<br />
::::::* the [[Oroboros_contact#Oroboros_Bioenergetics_Adventurers |Oroboros Bioenergetics Adventurers]]<br />
::::::* the [[Oroboros_contact#Oroboros_Quality_Management_Angels |Oroboros Quality Management Angels]]<br />
::::::* the [[Oroboros_contact#Oroboros_Leadership_Group |Oroboros Leadership Group]]<br />
<br />
|}<br />
|}<br />
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*'''Jan-04 Sa'''<br />
:::: [[Image:O2k-News icon.jpg|60px|link=https://wiki.oroboros.at/index.php/Oroboros_News | News]] The [[Oroboros Ecosystem Agenda 2020]] is launched as a signature of the [[Oroboros Ecosystem]].<br />
<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] [https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(19)33170-8/fulltext?dgcid=raven_jbs_etoc_email 2020: a critical year for women, gender equity, and health - a Lancet Editorial] <br />
<br />
::::* One month ago<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:: '''NextGen-O2k SME2 coaching Orily Pratt part 2'''<br />
<br />
<hr><br />
<br />
{|<br />
|[[File:2019-12-04 Coaching with Orily Pratt.jpg|left|250px|MitoFit Guest Seminar]] On '''December 03/04''' the second part of the '''SME phase 2 Coaching for the [[NextGen-O2k]] project''' with '''Orily Pratt''' (Tel Aviv, IL) took place in Innsbruck. <br />
<br/><br />
<br/><br />
<br />
:» Wednesday, 4th of December; Oroboros Instruments, Schoepfstrasse 18, MiPArt, Innsbruck, Austria.<br />
<br />
|}<br />
|}<br />
<br />
<br />
== Stay alert ==<br />
{{Oroboros Ecosystem Agenda list}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=205881
Template talk:Next oroboros ecosystem agenda
2020-08-06T14:08:35Z
<p>Antunes Diana: /* Next */</p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
*'''Aug-20 Thr'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to so with the [[Q-Module]] that we are developing as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project. <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
<br />
Suggestion 2:<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to so with the new module that we are developing as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Aug-25 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange by [[ANT]] using [[MgG]] with the O2k''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Aug-11 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Ocean warming affects ET capacity of mitochondria from developing eggs of Atlantic herring'''<br><br />
:from the O2k-Network »[[DE Bremerhaven Mark FC]]«<br />
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
* '''Aug-17 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you observe a high O2 flux in the closed chamber prior addition of sample? '''<br><br />
This might be due to [[biological contamination]] of the O2k-chamber. See how to clean it properly. <br><br />
- [[O2k-Chamber MitoPedia#Cleaning the glass chamber]] and [[MiPNet19.03 O2k-cleaning and ISS]]<br />
|}<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template_talk:Next_oroboros_ecosystem_agenda&diff=205869
Template talk:Next oroboros ecosystem agenda
2020-08-06T08:02:50Z
<p>Antunes Diana: </p>
<hr />
<div>__TOC__<br />
<br />
::::[[User:Antunes Diana|Antunes Diana]] ([[User talk:Antunes Diana|talk]]) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:<br />
<pre style="display: inline-block;"><br />
{|<br />
| logos<br />
| title in bold <br><br />
- link<br />
|}<br />
</pre><br />
<br />
''e.g.,''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?''' <br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._signal_in_O2_instrumental_BG Instrumental background]« and »[[TIP2k Filter Papers]]«<br />
|}<br />
:::: ''See the scheme'':<br />
<br><br />
[[Image:TEMPLATE ALERT.png|none|900px|Agenda/Alerts body text format]]<br />
<br />
== Bioblast alert 2020 ==<br />
Prep for circular done here: [[Talk:Bioblast alert 2020]]<br />
<br />
== MitoPedia alert ==<br />
<br />
<br />
=== Next ===<br />
*'''Aug-20 Thr'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to so with the [[Q-Module]] that we are developing as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project. <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
<br />
Suggestion 2:<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Would you like to simultaneously monitor O<sub>2</sub> flux and the [[Q redox state|redox state]] of the [[Q-junction]] in isolated mitochondria? Soon you will be able to so with the new module that we are developing as a part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project: <br><br />
<br><br />
- »'''[[Q-Module]]'''« <br />
|}<br />
<br />
== O2k-Brief alert ==<br />
<br />
=== Next ===<br />
<br />
* '''Aug-25 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Measuring ATP-ADP exchange by [[ANT]] using [[MgG]] with the O2k''' <br><br />
: from the O2k-Network »[[HU Budapest Chinopoulos C]]« <br><br />
* Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. [[Chinopoulos 2014 Methods Enzymol|»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Publications alert ==<br />
<br />
=== Next ===<br />
* '''Aug-11 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Ocean warming affects ET capacity of mitochondria from developing eggs of Atlantic herring'''<br><br />
:from the O2k-Network »[[DE Bremerhaven Mark FC]]«<br />
*Leo E, Dahlke FT, Storch D, Poertner HO, Mark FC (2018) Impact of Ocean Acidification and Warming on the bioenergetics of developing eggs of Atlantic herring Clupea harengus. Conserv Physiol 6:coy050. [[Leo 2018 Conserv_Physiol |»Bioblast link«]]<br />
|}<br />
<br />
== O2k-Open Support alert ==<br />
<br />
=== Next ===<br />
<br />
::::* While in Home Office: [[User:Gnaigere|Gnaigere]] ([[User talk:Gnaigere|talk]]) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda '''analysis-linked''' O2k-Open Support cases during this time, and collect real-time operation cases for future releases.<br />
<br />
* '''Aug-10 Mo'''<br />
<br />
=== Removed for correction ===<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you perform the ''leaky chamber test'' after chamber assembly? '''<br><br />
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the ''Leaky chamber test'' - especially after chamber reassembly. <br><br />
- »[[Talk:O2k-Chamber_MitoPedia#Leaky_Chamber|Leaky chamber test]]« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«<br />
|}<br />
<br />
== O2k-Open Support alert circulars ==<br />
<br />
=== O2k-Open Support alert circular 2020(07): in prep ===<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters|filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* For the question, do we use the term Fluorescence-Sensor or Smart Fluo-Sensors or fluorescence sensors which may include both Smart Fluo-Sensors (O2k-Fluo Smart-Module, https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/) and Fluorescence-Sensors (O2k-Fluo LED2-Module, https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/).<br />
::::* I modified in the former text the text displayed for filters, hydrogen peroxide, mitochondrial membrane potential and calcium.<br />
::::* Filters or LED filters and photodiode filters?<br />
::::* The link from the filters should go to which page? [[Filters]] or https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ or https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set?<br />
::::* Do we include in the entry the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?<br />
::::* In the last links, the first one goes to the "Select the Fluorescence-Sensors" page, but the second one does not go to the selection of filters.<br />
::::* The link to "Select the Fluorescence-Sensors" goes to the O2k-Fluo LED2-Module and not to the section "Select the Smart Fluo-Sensors"<br />
::::* Do we want to mention only the O2k-Fluo_Smart-Module or also the O2k-Fluo LED2-Module?<br />
<br />
:::* Suggestions:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors (with the [[O2k-Fluo_Smart-Module|O2k-Fluo Smart-Module]] or with the [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module|O2k-Fluo LED2-Module]) and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different Smart Fluo-Sensors and LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set| Selecting a Filter Set]'''«<br />
|}<br />
<br />
::::Comments by Timi:<br />
:::::Suggestion:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module| Selecting a Filter Set]]'''«<br />
|}<br />
<br />
:::::Arguments:<br />
::::::• It refers generally to both O2k-Fluo Smart-Modul and O2k-Fluo LED2-Modul, therefore, I would write fluorescence sensors.<br />
::::::• The link for Selecting a Filter Set what you put is not appropriate because it goes to a discussion page what we have not published so far, therefore, I would stick to the links in the original alerts. <br />
::::::• The MitoPedia:Fluorometry discussion page does not contain new information. It it summary of information of all fluo-related pages. Therefore, you can find the same information on an other page. <br />
::::::• In the original link the link of Selecting a Filter Set goes to the table with the sensors and filters and not to the listed points. <br />
<br />
:::* Comments by Caro:<br />
::::* Yes, I agree that the content of one of the suggestions which I provided is more appropiated. <br />
::::* The question of the entry refers to how to select the appropiate fluorescence sensors and filters. At the end, the first link goes to the section Select the Smart Fluo-Sensors (this is good). The second link does not go to a page which helps with the second question (Selecting a Filter Set), it goes to the O2k-Fluo LED2-Module setup (what about the users with the O2k-FluoRespirometer) and then you can click in another link which goes to a page where some information about the filters can be found inside of the mounting filter section (which is not selecting a filter), and it is not as complete as it is shown in the link [[Talk:MitoPedia:_Fluorometry#Selecting_a_Filter_Set]]. Therefore, if I am an user, I do not see the question of the entry fixed. Why the content of the selection of filters is not moved to a front page?<br />
<br />
:::* Final version:<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module O2k-Fluo LED2-Module]) allows for measurement of [[Hydrogen peroxide|hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential|mitochondrial membrane potential]], [[Calcium|calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[https://wiki.oroboros.at/index.php/O2k-Fluo_LED2-Module#Fluorophores Selecting a Filter Set]'''«<br />
|}<br />
<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that some chemicals are incompatible with the Amplex UltraRed assay?''' <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
:::* Comments by Caro:<br />
::::* Question in bold as the other entries.<br />
<br />
== In preparation ==<br />
== For discussion ==<br />
''Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 ('''if''' the specific alert section is yet to be attributed)''<br />
<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Controlled stability of the [https://www.oroboros.at/index.php/product/mitokit-cii MitoKit-CII] based on HPLC and LCMS analysis: No degradation of [[MitoKit-CII_MitoPedia| MitoKit-CII]] compounds [[MitoKit-CII/Succinate-nv| MitoKit-CII/Succinate-nv (NV118)]] and [[MitoKit-CII/Malonate-nv| MitoKit-CII/Malonate-nv (NV161)]] for following storage conditions:<br />
* Proven -80 °C solid stability for more than 3 years (3 years 7 months)<br />
* Proven -20 °C solid stability for 1 year<br />
* Proven DMSO stability at room temperature for 1 week<br />
* Proven DMSO stability at -20 °C for 6 months (ongoing)<br />
|}<br />
<br />
== 2021 ==<br />
<br />
::::* 2021-01-06 https://www.jstor.org/stable/1706346?seq=1<br />
<br />
== Postponed events ==<br />
<br />
:::: [[File:FAT4BRAIN logo.png|70px|FAT4BRAIN|link=FAT4BRAIN School Riga LV]] »<big>'''[[FAT4BRAIN School Riga LV | FAT4BRAIN School Riga LV IOC147]]'''</big>«<br />
<br />
:::: »<big>'''[[APS2020 Chicago US]]'''</big>«<br />
<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.09 IOC San Diego CA US |<big>'''San Diego CA US'''</big>]]<br />
<br />
::::* Abstracts deadline [https://www.cebitec.uni-bielefeld.de/events/conferences/575-algal-biotech-202 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=File:TEMPLATE_ALERT.png&diff=205868
File:TEMPLATE ALERT.png
2020-08-06T08:02:00Z
<p>Antunes Diana: Antunes Diana uploaded a new version of File:TEMPLATE ALERT.png</p>
<hr />
<div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Talk:Bioblast_alert_2020&diff=205852
Talk:Bioblast alert 2020
2020-08-05T11:56:01Z
<p>Antunes Diana: </p>
<hr />
<div>== In prep ==<br />
<br />
:{|<br />
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]<br />
| '''Did you miss the O2k-Webinar on O2k-Applications: simultaneous measurement of O₂ flux and H₂O₂ production presented by [[Komlodi Timea|Dr. Tímea Komlódi]]?''' <br><br />
Watch the recorded presentation. »'''[https://www.youtube.com/watch?v=IJhhvhn_mEM&feature=youtu.be O2k-Webinar: Simultaneous measurement of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> production]'''«<br><br />
Read the follow-up questions and answers. »'''[https://wiki.oroboros.at/index.php/Talk:O2k-Webinars O2k-Webinar Q&A ]'''«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
<br />
== On hold ==</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Talk:Bioblast_alert_2020&diff=205851
Talk:Bioblast alert 2020
2020-08-05T10:10:43Z
<p>Antunes Diana: /* In prep */</p>
<hr />
<div>== In prep ==<br />
<br />
:{|<br />
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]<br />
| '''Did you miss the O2k-Webinar on O2k-Applications: simultaneous measurement of O₂ flux and H₂O₂ production presented by [[Komlodi Timea|Dr. Tímea Komlódi]]?''' <br><br />
Watch the recorded presentation. »'''[https://www.youtube.com/watch?v=IJhhvhn_mEM&feature=youtu.be O2k-Webinar: Simultaneous measurement of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> production]'''«<br><br />
Read the follow-up questions and answers. »'''[https://wiki.oroboros.at/index.php/Talk:O2k-Webinars O2k-Webinar Q&A ]'''«<br />
|}<br />
----<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
<br />
== On hold ==</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPediaAlert2020_Archive&diff=205850
MitoPediaAlert2020 Archive
2020-08-05T09:32:37Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
== Aug 2020 ==<br />
<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|}<br />
<br />
<br />
== Previous MitoPedia Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
----<br />
* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
----<br />
<br />
* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
----<br />
* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
<br />
----<br />
* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
<br />
== Jun 2020 ==<br />
* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
<br />
* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
<br />
* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_Red_method Incompatible substrates]'''«<br />
|}<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
<br />
* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
<br />
<br />
=== May 2020 ===<br />
<br />
* '''May-28 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
<br />
* '''May-21 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
|As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
* '''May-14 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
<br />
* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''<br />
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br><br />
- Learn more about - »'''[[Malate-anaplerotic pathway control state|malate-anaplerotic pathway control state]]'''« <br><br />
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« <br><br />
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
=== Apr 2020 ===<br />
<br />
* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]]. <br><br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
<br><br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. <br><br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
<br><br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br><br />
- »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
<br><br />
* '''Apr-08 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. <br><br />
- »'''[[SUIT-010 |SUIT-010]]'''«<br />
|}<br />
<br><br />
* '''Apr-02 Th''' <br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' <br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-16 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. <br><br />
- »'''[[SUIT-003 |SUIT-003]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. <br><br />
- »'''[[Blebbistatin]]'''«<br />
|}<br />
<br><br />
* '''Mar-06 Fr'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br><br />
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? <br><br />
- »'''[[Rapamycin]]'''«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-26 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. <br><br />
- »'''[[Noncoupled respiration]]'''«<br />
|}<br />
<br><br />
* '''Feb-24 Mo'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| More research is needed on the impact of viral infection on mitochondrial function. <br><br />
- »'''[[Viruses and mitochondrial medicine]]'''«<br />
|}<br />
<br><br />
* '''Feb-19 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. <br><br />
- »'''[[Spermidine]]'''«<br />
|}<br />
<br><br />
* '''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Why do we fight gas bubbles? <br><br />
- Dive deep into »'''[[Oxygen solubility]]'''«<br />
|}<br />
<br><br />
* '''Feb-12 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| What do metformin, melatonin and other bioactive mitObesity compounds have in common? <br><br />
- »'''[[MitObesity drugs]]'''«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-16 Th'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Oxygen flux]]''' <br><br />
- which SI units should be used?<br />
|}<br />
<br><br />
<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Did you ever think that units for body mass could be an issue? <br><br />
- »'''[[Body mass]]'''«<br />
|}<br />
<br><br />
* '''Jan-08 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Oroboros_Ecosystem_Agenda_2020_Archive&diff=205849
Oroboros Ecosystem Agenda 2020 Archive
2020-08-05T09:31:28Z
<p>Antunes Diana: </p>
<hr />
<div>{{OROBOROS header page name}}<br />
[[File:Oroboros_EcosystemAgenda_2020.png|right|560px|Oroboros Ecosystem Agenda 2020]]<br />
{{Template:OROBOROS Slogan}}<br />
<br />
::::<big>» [[Oroboros Ecosystem Agenda 2020 |Current Oroboros Ecosystem Agenda 2020]]</big><br />
<br />
__TOC__<br />
2020 Week 31<br />
<br />
* '''Jul-27 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''<br />
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br><br />
- »[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«<br />
|} <br />
<br />
----<br />
* '''Jul-28 Tu''' - World Hepatitis Day<br />
:{|<br />
<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Insights into Hepatitis C virus-linked mitochondrial dysfunction''' <br><br />
: from the O2k-Network »[[IT Foggia Capitanio N]]« <br><br />
* Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60. [[Ripoli 2009 J Virol|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-29 We''' <br />
<br />
----<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
----<br />
* '''Jul-31 Fr'''<br />
<br />
2020 Week 30<br />
<br />
* '''Jul-20 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''How can you compare respirometric experiments using an internal normalization?'''<br><br />
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”. <br />
<br><br />
- »[[Flux control ratio]]«<br />
<br />
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
----<br />
* '''Jul-21 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Absence of AMPK in skeletal muscle impacts mitochondrial content and respiration in addition to mitigating glucose intolerance induced by a high-fat diet'''<br><br />
:from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MN Minneapolis Hughey C]]« <br />
*Lantier L, Williams AS, Williams IM, Guerin A, Bracy DP, Goelzer M, Foretz M, Viollet B, Hughey CC, Wasserman DH (2020) Reciprocity between skeletal muscle AMPK deletion and insulin action in diet-induced obese mice. Diabetes [Epub ahead of print]. [[Lantier 2020 Diabetes|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Jul-22 We''' <br />
<br />
<br />
----<br />
* '''Jul-23 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:O2k-Webinars icon.png|left|80px|MitoPedia]]<br />
| '''First [[O2k-Webinars|O2k-Webinar]] - O2k-Applications: H<sub>2</sub>O<sub>2</sub> production by [[Komlodi Timea|Dr. Tímea Komlódi]] ''' <br />
|}<br />
----<br />
* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
<br />
<br />
2020 Week 29<br />
<br />
* '''Jul-13 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br><br />
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. <br />
For more information, see:<br />
- »[[Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP|Copy from file]]«<br />
|}<br />
----<br />
* '''Jul-14 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured ''Jatropha curcas'' cells'''<br><br />
<br />
:from the O2k-Network »[[BR Ribeirao Preto Alberici LC]]« <br />
*Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on ''Jatropha curcas'' cell culture. Plant Physiol Biochem 136:92-97. [[Vicentini 2019 Plant Physiol Biochem|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-15 We''' <br />
<br />
<br />
----<br />
* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper fluorescence sensors and filter set?''' <br><br />
Using different fluorescence sensors, LED and photodiode filters, the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] (or [https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ O2k-Fluo LED2-Module]) allows for measurement of [[hydrogen peroxide]], [[ATP]], [[mitochondrial membrane potential]], [[calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors|Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module|Selecting a Filter Set]]'''«<br />
|}<br />
<br />
----<br />
* '''Jul-17 Fr'''<br />
----<br />
<br />
<br />
2020 Week 28 <br />
<br />
* '''Jul-06 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br><br />
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear.<br />
For more information, see:<br><br />
- »[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]« and »[[DatLab error messages]] «<br />
|}<br />
----<br />
* '''Jul-07 Tu''' <br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice'''<br><br />
:from the O2k-Network »[[FR Lille Duez H]]« <br />
*Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. [[Ayari 2020 Commun Biol|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-08 We''' <br />
<br />
----<br />
* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
----<br />
* '''Jul-10 Fr'''<br />
<br />
== Jun 2020 ==<br />
2020 Week 27<br />
<br />
* '''Jun-29 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br><br />
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br><br />
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«<br />
|}<br />
----<br />
* '''Jun-30 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice'''<br><br />
:from the O2k-Network »[[LV Riga Makrecka-Kuka M]]« <br />
*Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. [[Makrecka-Kuka 2020 Cardiovasc Drugs Ther|»Bioblast link«]]<br />
|}<br />
----<br />
* '''Jul-01 We''' <br />
:{|<br />
| width="100" align="center" |[[Image:TRANSMIT Acronym.jpg|70px|link=https://wiki.oroboros.at/index.php/TRANSMIT#TRANSMIT_dissemination |TRANSMIT]]<br />
|'''Kick-off of the TRANSMIT crowdfunding campaign: NOW'''<br><br />
:Bring the world of scientific research closer to the world of patients by supporting four cancer patients’ associations.<br />
<br />
:- »[https://www.ideaginger.it/progetti/now.html https://www.ideaginger.it/progetti/now.html]«<br />
|}<br />
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* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
<br />
----<br />
* '''Jul-03 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|'''Mitochondrial H<sub>2</sub>O<sub>2</sub> flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration''' <br><br />
: from the O2k-Network »[[US CO Fort Collins Chicco AJ]]« <br><br />
* Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. [[Li Puma 2020 Am J Physiol Regul Integr Comp Physiol|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
<br />
2020 Week 26<br />
<br />
* '''Jun-22 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Did you know how a stirrer test can save your experiments?'''<br><br />
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
----<br />
* '''Jun-23 Tu'''<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''ASCs-exosomes: a potential therapy to restore mitochondrial function in amyotrophic lateral sclerosis'''<br><br />
:from the O2k-Network »[[IT Verona Calabria E]]« <br />
*Calabria E, Scambi I, Bonafede R, Schiaffino L, Peroni D, Potrich V, Capelli C, Schena F, Mariotti R (2019) ASCs-exosomes recover coupling efficiency and mitochondrial membrane potential in an ''in vitro'' model of ALS. Front Neurosci 13:1070. [[Calabria_2019_Front_Neurosci|»Bioblast link«]]<br />
|}<br />
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* '''Jun-24 We'''<br />
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* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
<br />
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* '''Jun-26 Fr'''<br />
<br />
{|<br />
| width="110" align="center" | <br />
<br />
[[Image:MitoGlobal.jpg|80px|left|MitoGlobal]]<br />
<br />
| Join and disseminate the [https://mitowomen.org/index.html '''“Women in Mitochondria”'''] initiative which aims to make the women working in the mitochondrial field more visible, contributing to accelerating equality and diversity. <br><br />
|}<br />
<br />
2020 Week 25<br />
<br />
* '''Jun-15 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
<br />
----<br />
* '''Jun-16 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''A new potential anti-aging therapeutic target: circadian regulation of mitochondrial uncoupling'''<br><br />
:from the O2k-Network »[[CA Montreal Hepple RT]]« <br />
*Ulgherait M, Chen A, McAllister SF, Kim HX, Delventhal R, Wayne CR, Garcia CJ, Recinos Y, Oliva M, Canman JC, Picard M, Owusu-Ansah E, Shirasu-Hiza M (2020) Circadian regulation of mitochondrial uncoupling and lifespan. Nat Commun 11:1927. [[Ulgherait 2020 Nat Commun|»Bioblast link«]]<br />
|}<br />
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* '''Jun-17 We''' <br />
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* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
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* '''Jun-19 Fr'''<br />
<br />
2020 Week 24<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
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* '''Jun-09 Tu''' <br />
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* '''Jun-10 We'''<br />
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* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_UltraRed_assay Incompatible substrates]'''«<br />
|}<br />
<br />
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* '''Jun-15 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|''' Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
----<br />
* '''Jun-12 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Oxygen consumption by intact platelets positively correlates with the concentration of CoQ<sub>10</sub> in the platelets of CKD patients''' <br><br />
: from the O2k-Network » [[ SK Bratislava Sumbalova Z]] « <br><br />
* Gvozdjáková A, Sumbalová Z, Kucharská J, Komlósi M, Rausová Z, Vančová O, Számošová M, Mojto V (2020) Platelet mitochondrial respiration, endogenous coenzyme Q<sub>10</sub> and oxidative stress in patients with chronic kidney disease. Diagnostics (Basel) 10:E176. [[Gvozdjakova 2020 Diagnostics (Basel)|»Bioblast link«]]<br />
<br />
|}<br />
<br />
<br />
2020 Week 23 <br />
<br />
* '''Jun-01 Mo''' <br />
:{|<br />
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«<br />
|}<br />
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* '''Jun-02 Tu''' <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers'''<br><br />
:from the O2k-Network »[[AT Innsbruck Gnaiger E]]« , »[[CA Rimouski Blier PU]]« and »[[CA Edmonton Lemieux H]]« <br />
*Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. [[Lemieux 2017 Sci Rep|»Bioblast link«]]<br />
|}<br />
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* '''Jun-03 We''' <br />
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* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Mitochondria show variable preferences for [[GM|glutamate & malate]] or [[PM|pyruvate & malate]] to support NADH-linked respiration, depending on species, tissues, and pathophysiological state.''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to compare respiratory capacities supported by these NADH-linked fuel substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
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* '''Jun-05 Fr'''<br />
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== May 2020 ==<br />
2020 Week 22 <br />
<br />
* '''May-25 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br><br />
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br><br />
- »[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«<br />
|}<br />
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* '''May-26 Tu''' <br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Remodeling of OXPHOS in prostate cancer to potentially damaging mtDNA mutations and differential expression of mt-genes ''' <br><br />
: from the O2k-Network » [[ AT Innsbruck Gnaiger E]] « and » [[ AT Innsbruck Oroboros]] « <br><br />
* Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 11:1487. [[Schoepf 2020 Nat Commun|»Bioblast link«]]<br />
<br />
|}<br />
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* '''May-27 We''' <br />
:{| <br />
| width="100" align="center" |[[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]]<br />
|The decline of mitochondrial respiratory function is strongly associated with obesity. This may play a potential role in determining corona virus severity. Read more: https://wiki.oroboros.at/index.php/Viruses_and_mitochondrial_medicine<br />
<br />
To fight COVID-19 the world urgently needs effective and safe diagnostics, treatments and vaccines. The EU is organizing a Global Response pledging effort to finance life-saving solutions. Join the marathon and spread the word: https://europa.eu/global-response/#UnitedAgainstCoronavirus<br />
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|}<br />
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* '''May-28 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
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* '''May-29 Fr'''<br />
<br />
:{|<br />
<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''F<sub>1</sub>F<sub>O</sub>-ATP synthase regulates alternative oxidase in ''Trypanosoma brucei'' '''<br><br />
:from the O2k-Network »[[BR Sao Paulo Silber AM]]« <br />
*Alberto Luévano-Martínez L, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. [[Alberto Luevano-Martinez 2020 FEBS Lett|»Bioblast link«]]<br />
|}<br />
<br />
2020 Week 21 <br />
<br />
* '''May-18 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Did you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br><br />
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br><br />
- »[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«<br />
|}<br />
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* '''May-19 Tu''' <br />
:{|<br />
| width="100" align="center"|[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells'''«<br><br />
:from the O2k-Network »[[Charles Anne-Laure]]« »[[Geny Bernard]]«<br />
*Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard <br>(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 10.1016/j.anai.2016.11.026. [[Qi 2017 Ann Allergy Asthma Immunol|»Bioblast link«]]<br />
|}<br />
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* '''May-20 We''' <br />
:{|<br />
| width="100" align="center"|[[Image:SI-units.png|65px|link=https://wiki.oroboros.at/index.php/BEC2020.1_doi10.26124bec2020-0001.v1#SI_units|SI Units]]<br />
|'''SI units'''<br><br />
One year ago, on [https://www.euramet.org/publications-media-centre/news/?tx_news_pi1%5Bnews%5D=787&tx_news_pi1%5Baction%5D=detail&tx_news_pi1%5Bcontroller%5D=News World Metrology Day] (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of '''Mitochondrial physiology''' and with the launch of an innovative journal — '''[[Bioenergetics Communications]]'''.<br />
|}<br />
<br />
:{|<br />
| width="100" align="center"|[[Image:BEC-logo.png|100px|link=https://wiki.oroboros.at/index.php/Bioenergetics_Communications |Bioenergetics Communications]]<br />
|MitoEAGLE ''Consortium Communication'': '''Mitochondrial physiology''' ― Published online: 2020-05-20.<br />
Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1<br><br />
» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1<br />
|}<br />
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* '''May-21 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow one to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
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* '''May-22 Fr'''<br />
<br />
2020 Week 20<br />
<br />
* '''May-11 Mo'''<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.'''<br><br />
Learn more about steady states including performance and mark setting for data analysis <br><br />
- »[[Steady state]]«<br />
|}<br />
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* '''May-12 Tu''' <br />
:{|<br />
| width="100" align="center" | [[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
| '''Mitochondria - a driving force in adipocyte differentiation''' <br><br />
:from the O2k-Network »[[CZ Pilsen Kuncova J]]« <br><br />
* Kladnická I, Čedíková M, Kripnerová M, Dvořáková J, Kohoutová M, Tůma Z, Müllerová D, Kuncová J (2019) Mitochondrial respiration of adipocytes differentiating from human mesenchymal stem cells derived from adipose tissue. Physiol Res 68:287-96. [[Kladnicka 2019 Physiol Res|»Bioblast link«]]<br />
|}<br />
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* '''May-13 We'''<br />
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* '''May-14 Th'''<br />
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:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
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* '''May-15 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mouse ''' <br><br />
: from the O2k-Network »[[ FI Helsinki Jacobs HT]]« , »[[ DE Magdeburg Gellerich FN]]« , »[[FI Tampere Dufour E]]« , »[[DE Magdeburg Debska-Vielhaber G]]« and »[[DE Frankfurt Wittig I]]«<br><br />
* Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet Ma, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich FN, Schlueter KD, Jacobs HT (2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48. [[Szibor 2020 J Cell Mol Med|»Bioblast link«]]<br />
|}<br />
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* '''May-17 Su''' World Hypertension Day <br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|'''Mitochondrial DNA enhances the hypoxia-induced cardioprotective effect in hypertensive rats'''<br><br />
:from the O2k-Network »[[CZ Prague Houstek J]]« and »[[CZ Prague Kalous M]]«<br />
*Neckar J, Svatonova A, Weissova R, Drahota Z, Zajickova P, Brabcova I, Kolar D, Alanova P, Vasinova J, Silhavy J, Hlavackova M, Tauchmannova K, Milerova M, Ostadal B, Cervenka L, Zurmanova J, Kalous M, Novakova O, Novotny J, Pravenec M, Kolar F (2017) Selective replacement of mitochondrial DNA increases the cardioprotective effect of chronic continuous hypoxia in spontaneously hypertensive rats. Clin Sci (Lond) 131(9):865-881. [[Neckar 2017 Clin Sci (Lond)|»Bioblast link«]]<br />
|}<br />
2020 Week 19<br />
<br />
* '''May-04 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you load the correct instrumental background correction according to the actual range of experimental O<sub>2</sub> concentrations?'''<br><br />
Perform and apply the instrumental background test:<br><br />
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«<br />
|}<br />
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* '''May-05 Tu''' <br />
::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]''' Expression of alternative oxidase prevents reverse electron transfer'''<br />
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]« »[[DE Magdeburg Gellerich FN]]« »[[FI Tampere Dufour E]]« »[[DE Magdeburg Debska-Vielhaber G]]« »[[DE Frankfurt Wittig I]]« and »[[UK Brighton Moore AL]]«<br />
::::::* Szibor Marten, Gainutdinov Timur, Fernandez-Vizarra Erika, Dufour Eric, Gizatullina Zemfira, Debska-Vielhaber Grazyna, Heidler Juliana, Wittig Ilka, Viscomi Carlo, Gellerich Frank Norbert, Moore Anthony L (2019) Bioenergetic consequences from xenotopic expression of a tunicate AOX in mouse mitochondria: switch from RET and ROS to FET. Biochim Biophys Acta Bioenerg 1861:148137. [[Szibor 2019 Biochim Biophys Acta Bioenerg|»Bioblast link«]]<br />
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* '''May-06 We''' <br />
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* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
| Quantify '''[[Fatty acid oxidation pathway control state|fatty acid oxidation capacity (FAO, F-pathway)''']], avoiding a common artefact of overestimation'''. Malate anaplerotic pathways ([[Malic enzyme|see '''mt-malic enzyme''']]) are active in various tissues and cultures cells, which requires caution: »'''[[Malate-anaplerotic pathway control state]]'''«<br />
<br />
- '''DatLab 7.4''' provides a step-by-step guide to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''«<br />
<br />
- Since [[Fatty acid oxidation pathway control state|F-pathway capacity]] can be overestimated due to anaplerotic reactions, a reference protocol was designed to avoid this artefact - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
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* '''May-08 Fr'''<br />
<br />
== Apr 2020 ==<br />
<br />
2020 Week 18<br />
<br />
* '''Apr-27 Mo''' <br />
:::: [[Image:O2k-Support.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''<br />
::::::: The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub> concentrations. - »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«<br />
<br />
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* '''Apr-28 Tu''' <br />
::::[[Image:O2k-Publications.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Cytosolic calcium controls extramitochondrial pyruvate generation and mitochondrial pyruvate uptake'''<br />
::::::: - from the O2k-Network »[[FI Helsinki Jacobs HT]]«, »[[DE Magdeburg Gellerich FN]]« and »[[DE Magdeburg Debska-Vielhaber G]]« <br />
::::::* Szibor Marten, Gizatullina Zemfira, Gainutdinov Timur, Endres Thomas, Debska-Vielhaber Grazyna, Kunz Matthias, Karavasili Niki, Hallmann Kerstin, Schreiber Frank, Bamberger Alexandra, Schwarzer Michael, Doenst Torsten, Heinze Hans-Jochen, Leßmann Volkmar, Vielhaber Stefan, Kunz Wolfram S, Gellerich Frank Norbert (2020) Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply. J Biol Chem [Epub ahead of print]. - [[Szibor 2020 J Biol Chem|»Bioblast link«]]<br />
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* '''Apr-29 We''' <br />
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* '''Apr-30 Th'''<br />
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* '''May-01 Fr'''<br />
<br />
2020 Week 17<br />
* '''Apr-20 Mo''' <br />
<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial respiration medium MiR05 wins - KCl inhibits'''<br />
<br />
::::::: - from the O2k-Network »[[US TN Nashville Wasserman DH]]« and »[[US MI East Lansing Bazil JN]]«<br />
::::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]]<br />
<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|40px|Info: improve]] '''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''<br />
::::::: Unstable respiratory fluxes in OXPHOS might be due to substrates limitation - »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«<br />
<br />
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<br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature.<br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
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* '''Apr-22 We''' <br />
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* '''Apr-23 Th''' <br />
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* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]].<br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
<br /><br />
<br />
2020 Week 16<br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' You can quantify the ATPase activity in your mitochondrial preparation:'''<br />
- DatLab 7.4 provides a step-by-step guide through the SUIT-024 protocol of [[mitochondrial preparations]] - »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
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* '''Apr-14 Tu''' <br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Why are the oxygen levels different between chambers prior to my experimental run?'''<br />
::::::: After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations - »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«<br />
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* '''Apr-15 We''' <br />
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* '''Apr-16 Th'''<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Mitochondrial function could be an early peripheral marker for the detection of Alzheimer's disease'''<br />
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::::::: - from the O2k-Network »[[DE Frankfurt Eckert GP]]« »[[CH Basel Eckert A]]«<br />
::::::* Leuner K, Schulz K, Schütt T, Pantel J, Prvulovic D, Rhein V, Savaskan E, Czech C, Eckert A, Müller WE (2012) Peripheral mitochondrial dysfunction in Alzheimer's disease: focus on lymphocytes. Mol Neurobiol 46:194-204. - [[Leuner 2012 Mol Neurobiol|»Bioblast link«]] <br />
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* '''Apr-17 Fr'''<br />
2020 Week 15<br />
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* '''Apr-06 Mo''' <br />
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<big><big>{{Template:Humankind COVID-19}}</big></big><br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Physical activity has positive effects on mitochondria from patients with occupational burnout''' <br />
::::::: - from the O2k-Network »[[CH Basel Eckert A]]«<br />
::::::* Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - [[Brand 2020 J Clin Med|»Bioblast link«]]<br />
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* '''Apr-07 Tu''' <br />
:::: [[Image:Logo OROBOROS-DatLab.jpg|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] [[File:Blue-info.png|40px|Info: improve]] '''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?''''<br />
<br />
::::::: If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]]. - »[[SUIT: Browse DL-Protocols and templates]]«<br />
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* '''Apr-08 We''' <br />
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.'''<br />
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. - »'''[[SUIT-010 |SUIT-010]]'''«<br />
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* '''Apr-09 Th'''<br />
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* '''Apr-10 Fr'''<br />
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2020 Week 14<br />
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* '''Mar-30 Mo'''<br />
::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:''' »'''[[Enable DL-Protocol editing]]'''«<br />
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* '''Mar-31 Tu''' <br />
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* '''Apr-01 We''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Parkinson’s Disease Awareness Month:''' Dominant mutation in Parkinson’s Disease causes loss of protein function in mitochondria''' <br />
::::::: - from the O2k-Network »[[FR Angers Gueguen N]]« and »[[BE Leuven Spinazzi M]]«<br />
::::::* Cornelissen T, Spinazzi M, Martin S, Imberechts D, Vangheluwe P, Bird M, De Strooper B, Vandenberghe W (2020) CHCHD2 harboring the Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2. Hum Mol Genet [Epub ahead of print]. [[Cornelissen 2020 Hum Mol Genet|»Bioblast link«]]<br />
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[[File:Tony hickey garage.jpg|200px|thumb|Non-stoppable: Tony Hickey in home-office mode on high-resolution respirometry]]<br />
* '''Apr-02 Th''' <br />
::::[[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]'''Do you want to search the best [[SUIT]] protocol for your research questions?''' Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
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::::[[Image:O2k-Network.png|70px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] Creative solutions coming from the O2k-Network labs - » [[NZ Auckland Hickey AJ]] <br />
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* '''Apr-03 Fr'''<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Novel diurnal action of of mitochondrial stress-induced GDF15 in systemic metabolism regulation'''<br />
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::::::: - from the O2k-Network »[[DE Nuthetal Klaus S]]«<br />
::::::* Ost M, Igual Gil C, Coleman V, Keipert S, Efstathiou S, Vidic V, Weyers M, Klaus S (2020) Muscle-derived GDF15 drives diurnal anorexia and systemic metabolic remodeling during mitochondrial stress. EMBO Rep [Epub ahead of print]. - [[Ost 2020 EMBO Rep |»Bioblast link«]] <br />
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== Mar 2020 ==<br />
2020 Week 13<br />
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* '''Mar-23 Mo''' <br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''<br />
::::::: '''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br><br />
::::::: '''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''«<br />
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* '''Mar-24 Tu'''<br />
:::: [[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Tuberculosis Day: '''Mitochondria as target to ''Mycobacterium tuberculosis'' infection''' <br />
::::::: - from the O2k-Network »[[US NC Durham Li PA]]«<br />
::::::* Lu X, Williams Z, Hards K, Tang J, Cheung CY, Aung HL, Wang B, Liu Z, Hu X, Lenaerts AJ, Woolhiser L, Hastings C, Zhang X, Wang Z, Rhee KY, Ding K, Zhang T, Cook GM (2018) A pyrazolo[1,5-a]pyridine inhibitor of the respiratory cytochrome bcc complex for the treatment of drug-resistant tuberculosis. ACS Infect Dis 5:239-49. - [[Lu 2018 ACS Infect Dis |»Bioblast link«]]<br />
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* '''Mar-25 We''' <br />
:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] Preprint: '''An urgent call for research on COVID-19 and public vitamin D awareness'''<br />
::::::* Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. MitoFit Preprint Arch [[doi:10.26124/mitofit:200001]].<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Research findings made available via preprint servers before journal publication''' - [https://wellcome.ac.uk/press-release/sharing-research-data-and-findings-relevant-novel-coronavirus-covid-19-outbreak?sap-outbound-id=CF563AB7AA7FEDFB8731215C98A59C11790C0456&utm_source=hybris-campaign&utm_medium=email&utm_campaign=101_PBN2619_0000020211_GLS_101_aut_202003_Coronavirus_Forschung&utm_content=EN_internal_44988_20200325&mkt-key=005056A5C6311EE9999982134B93151D Sharing research data and findings relevant to the novel coronavirus (COVID-19) outbreak]<br />
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]]'''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4: »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br />
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* '''Mar-26 Th'''<br />
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* '''Mar-27 Fr'''<br />
<br />
2020 Week 12 <br />
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* '''Mar-16 Mo''' <br />
::::: [[Image:Logo_OROBOROS_INSTRUMENTS.jpg|left|60px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]] '''Oroboros operates from now on in home office mode - we stay connected.'''<br />
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:::: [[Image:SUIT icon.jpg|80px|left|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br />
::::::: - DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. - »'''[[SUIT-003 |SUIT-003]]'''«<br />
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* '''Mar-17 Tu'''<br />
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::::[[Image:O2k-Publications.jpg|60px|left|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ''' Zika virus infection leads to mitochondrial dysfunction''' <br />
::::::: - from the O2k-Network »[[BR Rio de Janeiro Galina A]]«<br />
::::::* Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK (2020) Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes. Sci Rep 10:1218. - [[Ledur 2020 Sci Rep |»Bioblast link«]]<br />
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* '''Mar-18 We''' <br />
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:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Exercise protects against the effects of aging on mitochondrial function'''<br />
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::::::: - from the O2k-Network »[[KR Busan Han J]]« »[[KR Incheon Kwak HB]]«<br />
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]] <br />
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* '''Mar-19 Th''' <br />
:::: [[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]] Home office mode is a world-wide protective strategy facing the COVID-19 pandemic. Supporting institutions and individual scientists in their home office strategy, during the time of crisis '''Oroboros Instruments provides free of charge copies of our newest software DatLab 7.4, for downloading at your personal or institutional PC at home'''.<br />
::::::: - » For the download link and password, please email [mailto:instruments@oroboros.at instruments@oroboros.at]<br />
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* '''Mar-20 Fr'''<br />
::::* '''Abstract submission deadline''' - »[[SHVM 2020 Halifax CA]]«<br />
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2020 Week 11<br />
* '''Mar-08 Su''' - '''[https://www.internationalwomensday.com/ International Women's Day]''' <br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Promising practices for addressing the underrepresentation of women in science, engineering, and medicine: opening doors.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Addressing the underrepresentation of women |»Bioblast link«]]<br />
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* '''Mar-09 Mo''' <br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] »<big>'''[[MitoEAGLE WG2 Innsbruck AT]]'''</big>« (Mar 09-13)<br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Protein-specific mitochondrial respiratory capacity is higher in women than men''' <br />
::::::: - from the O2k-Network »[[SE Stockholm Cardinale DA]]«<br />
::::::* Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133. - [[Cardinale 2018 Front Physiol |»Bioblast link«]]<br />
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* '''Mar-10 Tu'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Muscular exercise for training adipose tissue mitochondria''' <br />
::::::: - from the O2k-Network »[[DK Copenhagen Larsen S]]«<br />
::::::* Mendham Amy E, Larsen Steen, George Cindy, Adams Kevin, Hauksson Jon, Olsson Tommy, Fortuin-de Smidt Melony C, Nono Nankam Pamela A, Hakim Olah, Goff Louise M, Pheiffer Carmen, Goedecke Julia H (2020) Exercise training results in depot-specific adaptations to adipose tissue mitochondrial function. Sci Rep 10:3785. - [[Mendham 2020 Sci Rep |»Bioblast link«]]<br />
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* '''Mar-11 We'''<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. - »'''[[Blebbistatin]]'''«<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Let's talk COVID-19 - a new culture of communication in science.''' - [https://www.sciencemag.org/news/2020/02/completely-new-culture-doing-research-coronavirus-outbreak-changes-how-scientists Kay Kupferschmidt in Science]<br />
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::::[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you change the [[Smoothing|slope smoothing]] to 20 while operating the O2k with biological sample?'''<br />
::::::: - It is advantageous to decrease 'slope smoothing' to visualize changes of respiration more rapidly in O2k-experiments after calibration - especially before [[uncoupler titrations]]. - »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«<br />
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* '''Mar-12 Th'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] Multiple Sclerosis Month: '''Mitochondrial permeability transition pore protection as novel therapeutic approach in multiple sclerosis''' <br />
::::::: - from the O2k-Network »[[UK London Duchen MR]]«<br />
::::::* Warne J, Pryce G, Hill J, Shi X, Lennerås F, Puentes F, Kip M, Hilditch L, Walker P, Simone MI, Chan AW, Towers GJ, Coker A, Duchen MR, Szabadkai G, Baker D, Selwood D (2015) Selective inhibition of the mitochondrial permeability transition pore protects against neuro-degeneration in experimental multiple sclerosis. J Biol Chem 291:4356-73. - [[Warne 2015 J Biol Chem |»Bioblast link«]] <br />
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* '''Mar-13 Fr'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Men and women with Type 1 diabetes and skeletal muscle mitochondria''' <br />
::::::: - from the O2k-Network »[[CA Toronto Perry CG]]«<br />
::::::* Monaco CMF, Bellissimo CA, Hughes MC, Ramos SV, Laham R, Perry CGR, Hawke TJ (2020) Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes. Am J Physiol Endocrinol Metab 318:E44-E51. - [[Monaco 2020 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
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2020 Week 10<br />
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* '''Mar-02 Mo''' <br />
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] An amazing number of new [[O2k-Publications: Topics |O2k-Publications]] from China. - »'''[[O2k-Network Labs China]]'''«<br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Salty diet, cardiac hypertrophy, calcium overload, and mitochondrial dysfunction''' <br />
::::::: - from the O2k-Network »[[CN Chongqing Zhu Z]]«<br />
::::::* Lu Z, Cui Y, Wei X, Gao P, Zhang H, Wei X, Li Q, Sun F, Yan Z, Zheng H, Yang G, Liu D, Zhu Z (2018) Deficiency of PKD2L1 (TRPP3) exacerbates pathological cardiac hypertrophy by augmenting NCX1-mediated mitochondrial calcium overload. Cell Rep 24:1639-52. - [[Lu 2018 Cell Rep |»Bioblast link«]] <br />
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* '''Mar-03 Tu''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Rapamycin suppresses senescence in galactose versus glucose cell culture''' <br />
::::::: - from the O2k-Network »[[US PA Philadelphia Orynbayeva Z]]«<br />
::::::* Nacarelli T, Azar A, Altinok O, Orynbayeva Z, Sell C (2018) Rapamycin increases oxidative metabolism and enhances metabolic flexibility in human cardiac fibroblasts. Geroscience 40:243-56. - [[Nacarelli 2018 Geroscience |»Bioblast link«]]<br />
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:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? - »'''[[Rapamycin]]'''«<br />
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:::: [[Image:Gentle-Science_Ganesha.jpg|left|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] '''Advancing Open Science practices: stakeholder perspectives on incentives and disincentives.''' National Academies of Sciences, Engineering, and Medicine (2020) - [[National Academies of Sciences, Engineering, and Medicine 2020 Advancing Open Science practices |»Bioblast link«]] - The concept of [[Gnaiger 2019 MitoFit Preprint Arch Editorial |Preprints]] needs full attention in Open Science.<br />
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* '''Mar-04 We'''<br />
[[File:Platelet.jpg|right|200px]]<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Systemic mitochondrial bioenergetics in older adults - skeletal muscle and platelets''' <br />
::::::: - from the O2k-Network »[[US FL Orlando Goodpaster BH]]«<br />
::::::* Braganza A, Corey CG, Santanasto AJ, Distefano G, Coen PM, Glynn NW, Nouraie SM, Goodpaster BH, Newman AB, Shiva S (2019) Platelet bioenergetics correlate with muscle energetics and are altered in older adults. JCI Insight 5:128248. - [[Braganza 2019 JCI Insight |»Bioblast link«]] <br />
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[[File:Softdrink.jpg|right|100px]]<br />
:::: [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]] '''Sugar and fat - initial mitochondrial aging in the brain'''<br />
::::::: - from the O2k-Network »[[IT Naples Iossa S]]«<br />
::::::* Crescenzo R, Spagnuolo MS, Cancelliere R, Iannotta L, Mazzoli A, Gatto C, Iossa S, Cigliano L (2019) Effect of initial aging and high-fat/high-fructose diet on mitochondrial bioenergetics and oxidative status in rat brain. Mol Neurobiol 56:7651-63. - [[Crescenzo 2019 Mol Neurobiol |»Bioblast link«]] <br />
[[File:OroboPOS-Holder002.jpg|right|100px]]<br />
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''<br />
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«<br />
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* '''Mar-05 Th'''<br />
::::* '''Abstract submission opens''' - »[[Europhysiology 2020 Berlin DE]]«<br />
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:::: [[Image:MitoFit.jpg|left|50px|link=http://www.mitofit.org/index.php/MitoFit |MitoFit]] With a '''preprint''' you can communicate recent findings fast to the scientific community, secure a DOI, and receive input from other scientists even before submitting to a peer-reviewed journal. - »'''[[Preprint]]'''«<br />
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* '''Mar-06 Fr'''<br />
:::: [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] '''Explore metabolic profiles and specific modultations of mitochondrial pathways''' in health and disease with SUIT reference protocols. - »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
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== Feb 2020 ==<br />
<br />
2020 Week 9<br />
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* '''Feb-23 Su'''<br />
:::::::» '''[[2020 PaduaMuscleDays Padova IT]]''' - ''postponed to fall/winter 2020 due to coronavirus outbreak in Northern Italy''<br />
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* '''Feb-24 Mo''' <br />
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] More research is needed on the impact of viral infection on mitochondrial function. - »'''[[Viruses and mitochondrial medicine]]'''«<br />
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* '''Feb-25 Tu'''<br />
:::: [[Image:O2k-Network.png|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Network|O2k-Network]] High quality mitochondrial research is increasing rapidly in South Korea. - »'''[[O2k-Network Labs Republic of Korea]]'''«<br />
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::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''An active lifestyle protects cardiac mitochondria''' <br />
::::::: - from the O2k-Network: [[KR Busan Han J]], [[KR Incheon Kwak HB]]<br />
::::::* No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - [[No 2020 Pflugers Arch |»Bioblast link«]] <br />
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[[File:2020-02-26 MitoEAGLE WG4 Innsbruck AT.jpg|right|400px|thumb|MitoEAGLE WG4 retreat; left to right: [[Garcia-Souza Luiz F |Luiz]], [[Gnaiger Erich |Erich]], [[Aasander Frostner Eleonor |Eleonor]], [[Michalak Slawomir |Slawomir]]]]<br />
* '''Feb-26 We''' <br />
:::: [[File:MitoPedia.jpg|left|60px|MitoPedia]] It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. - »'''[[Noncoupled respiration]]'''«<br />
<br /><br />
<br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] '''[[MitoEAGLE WG4 Innsbruck AT]]''' (Feb 26-28)<br />
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* '''Feb-27 Th'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Regulation of mitochondrial oxygen kinetics by cytochrome ''c'' oxidase''' <br />
::::::: - from the O2k-Network: [[CZ Prague Houstek J]]<br />
::::::* Pajuelo-Reguera David, Čunátová Kristýna, Vrbacký Marek, Pecinová Alena, Houštěk Josef, Mráček Tomáš, Pecina Petr (2020) Cytochrome c oxidase subunit 4 isoform exchange results in modulation of oxygen affinity. Cells 9:E443. - [[Pajuelo-Reguera 2020 Cells |»Bioblast link«]] <br />
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* '''Feb-28 Fr'''<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''<br />
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«<br />
<br />
<br />
2020 Week 8<br />
<br />
* '''Feb-17 Mo''' <br />
:::: [[File:EBEClogo2020.jpg|80px|link=https://www.mitoglobal.org/index.php/EBEC2020_Marseille_FR]] '''Registration is open: [[EBEC2020_Marseille_FR |European Bioenergetics Conference 2020]]''', Marseille, France, 2020<br />
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* '''Feb-18 Tu''' <br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Why do we fight gas bubbles? » Dive deep into '''[[Oxygen solubility]]'''<br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis''' <br />
::::::: - from the O2k-Network »[[US MI Ann Arbor Beard DA]]«<br />
::::::* Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. - [[Tyrrell 2019 Circ Res |»Bioblast link«]]<br />
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* '''Feb-19 We''' <br />
:::: [[Image:MitoFit Preprint Arch.png|80px|link=MitoFit Preprint Arch]] - »'''[[MitoFit Preprint Arch]]'''« was initiated one year ago. We invite submissions of mitochondrial preprints.<br />
<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. - »'''[[Spermidine]]'''«<br />
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* '''Feb-20 Th'''<br />
:::: [[Image:Logo MitoFit s.jpg|40px|link=MitoFit Open Seminars]] - '''[[MitoFit Open Seminars|MitoFit Open Seminar]]''': [[Stefan_E|Eduard Stefan]]: “Decoding and Perturbation of ONCO-Kinase functions” and [[Torres-Quesada_O|Omar Torres Quesada]]: “'''[[MitoKIN]]''' - Systematic barcoding of kinase-controlled mitochondrial function”<br />
<br />
<br />
2020 Week 7<br />
<br />
*'''Feb-10 Mo''' <br />
::::* '''Early Bird Registration Deadline''' for [[ESSR 2020 Innsbruck AT]] <br />
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*'''Feb-12 We''' <br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] What do metformin, melatonin and other bioactive mitObesity compounds have in common? - »'''[[MitObesity drugs]]'''«<br />
<br />
<br />
:::: [[Image:O2k-Feedback.jpg|left|60px|link=http://wiki.oroboros.at/index.php/O2k-Feedback |O2k-Feedback]] ''I had a very pleasant experience at the work shop. I thank you and all the team members for devoting their time for us and teaching each step in a systematic manner.'' - '''[[Gayathri Narayanappa]]''' participaing in [[MiPNet25.03 IOC144 Innsbruck AT]]<br />
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*'''Feb-13 Th'''<br />
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<br />
2020 Week 6<br />
<br />
*'''Feb-03 Mo''' <br />
:::[[File:FFG-logo.png|150px|FFG]] '''<big>»Official start of the FFG funded project »[[MitoKIN|'''MitoKIN''']]«</big>''' <br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Blood cell mitochondrial function does not provide a general substitute for muscle biopsies''' <br />
::::::: - from the O2k-Network »[[US AR Little Rock Boersheim E]]« <br />
::::::* Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - [[Rose 2019 Am J Physiol Endocrinol Metab |»Bioblast link«]]<br />
<br />
::::* Do you know that the Oroboros O2k can also be used to study the mitochondrial dark respiration of microalgae and its response to climate change impact in the ocean? One of our customers, [https://wiki.oroboros.at/index.php/Tremblay_N Dr Nelly Tremblay] is using for the first time the [[SUIT-022]] protocol to study alternative oxidases in the marine model organism ''Phaeodactylum tricornutum''.<br />
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*'''Feb-04 Tu''' <br />
:::: [[Image:NextGenO2k logo.jpg|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.10 IOC and Distributor engagement Tokyo JP |'''MiPNet25.10 IOC and Distributor engagement Tokyo JP''']] (Feb 4-7)<br />
:::: [[File:O2k-brief.png|50px|link=https://wiki.oroboros.at/images/7/7b/Cronin_2018_Nature_O2k-brief.pdf|O2k-brief]] '''Cronin''' (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer. Nature - '''Lymphocytes''', '''Cancer''' - [http://wiki.oroboros.at/index.php/O2k-brief O2k-brief repository]<br />
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*'''Feb-05 We'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease'''<br />
::::::: - from the O2k-Network »[[US FL Boca Raton Khamoui AV]]«<br />
::::::* Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - [[Pena 2020 Int J Chronic Dis |»Bioblast link«]]<br />
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*'''Feb-06 Th'''<br />
:::: '''MitoFit Open Seminar'''<br />
:::::* 16:00-16:30 [[Beleza J|Jorge Beleza]]: Exercise performed during pregnancy as a preventive tool against transgenerational development of diabetes mellitus and NAFLD in the offspring. The role of epigenetic-mediated mechanisms. [[Media:MitoFitOpenSeminar_JorgeBeleza_20200206.pdf|»Abstract«]]<br />
:::::* 16:30-17:00 [[Pereira R|Rita Pereira]]: The Handball4Health Project : A community-based intervention programme<br />
<br />
<br />
== Jan 2020 ==<br />
<br />
2020 Week 5<br />
<br />
*'''Jan-27 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.06 IOC145 Innsbruck AT |<big>'''IOC145 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 27-29)<br />
<br />
:::: DSL-Seminar, 16:00<br />
:::::: [[Gnaiger E]]: mitObesity: compromised mitochondrial fitness in degenerative comorbidities of early aging.<br />
:::::: Univ.-Klinik für Visceral-, Transplantations- u. Thoraxchirurgie, Surgery building, 12th floor, north, seminar room<br />
<br />
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*'''Jan-28 Tu'''<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration of blood cells and cardiovascular disease''' <br />
::::::: - from the O2k-Network »[[FR Strasbourg Zoll J]]« <br />
::::::* Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - [[Alfatni 2020 J Clin Med |»Bioblast link«]]<br />
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*'''Jan-30 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Karabatsiakis_A|Alexander Karabatsiakis]]: Targeting the reversibility of mitochondrial changes in bioenergetics and biogenesis in Major Depressive Disorder by clinical routine antidepressant treatment.<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''<br />
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »[[Oxygen calibration - DatLab]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?'''<br />
<br />
::::::: Your air calibration is not effective until you set mark ‘R1’ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - »[[Oxygen calibration - DatLab]]« <br />
<br />
<br />
::::* '''Open Innovation''' in the NextGen-O2k software development DatLab 8. Here is a summary of [[MitoPedia: DatLab |MitoPedia]] pages including [[:Category:DatLab 8 perspectives |'''DatLab 8 perspectives''']]<br />
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*'''Jan-31 Fr'''<br />
::::* Deadline for [[APS2020_Chicago_US|APS2020]] Abstract submission for poster presentation.'''<br />
::::* [[2020 PaduaMuscleDays Padova IT |Call for EJTM Communications]] - deadline for manuscript submissions<br />
::::* Registration deadline for [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''<br />
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »[[Oxygen calibration - DatLab]]«<br />
<br />
2020 Week 4<br />
<br />
*'''Jan-20 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.03 IOC144 Innsbruck AT |<big>'''IOC144 Innsbruck AT'''</big>]] - O2k-Coaching Days (Jan 20-22)<br />
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*'''Jan-21 Tu'''<br />
::::* Mr Hai Wu (CEO of [[CN Beijing Huawei |Huawei]], Oroboros distributor in China) and Dr. Rupert Ecker (CEO of [https://www.tissuegnostics.com/en/TissueGnostics Vienna TissueGnostics]) join the O2k-Coaching Days for extending collaborative projects.<br />
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*'''Jan-23 Th''' - [[MitoFit_Open_Seminars|MitoFit Open Seminar]] - [[Gnaiger E|Erich Gnaiger]]: An improved index of obesity versus BMI - an issue of mitObesity and gender medicine in our modern world's obesogenic environment.<br />
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*'''Jan-24 Fr'''<br />
::::* Further circulars to invited speakers for submission of tentative titles for oral and poster presentations - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''<br />
::::::: Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. - »[[O2k configuration]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''<br />
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]«<br />
<br />
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?<br />
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]«<br />
<br />
2020 Week 3<br />
<br />
*'''Jan-14 Tu''' <br />
::::* Circular ITC Grants for [[2020 PaduaMuscleDays Padova IT]], [[6th EU-Cardioprotection Meeting 2020 Riga LV]] and [[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]] - [https://wiki.oroboros.at/images/a/af/ITC_Newsletter.pdf pdf]<br />
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*'''Jan-15 We''' <br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Statistical evaluation on replica in [[HRR]] with permeabilized skeletal muscle fibers''' <br />
::::::: - from the O2k-Network »[[AU Melbourne Stepto NK]]« <br />
::::::* Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - [[Jacques 2020 FASEB J |»Bioblast link«]]<br />
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*'''Jan-16 Th'''<br />
::::[[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Oxygen flux]]''' - which SI units should be used?<br />
<br />
::::[[File:MitoPedia.jpg|60px|MitoPedia]] Did you ever think that units for body mass could be an issue? - »'''[[Body mass]]'''«<br />
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*'''Jan-17 Fr''' <br />
::::[[Image:O2k-close.jpg|60px|link=http://wiki.oroboros.at/index.php/About_Oroboros |About Oroboros]] <big>'''HRR</big> - high-resolution respirometry and oxygen kinetics''' 10 years ago: '''Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010'''. - [[Scandurra_2010_Adv_Exp_Med_Biol |»Bioblast link«]]<br />
<br />
:::: [[Image:TRANSMIT Acronym.jpg|60px|TRANSMIT]] <big>'''[[TRANSMIT Symposium 2020 Brussels BE]]'''</big> (Jan 17-18)<br />
<br />
<br />
::::[[Image:O2k-Publications.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] '''Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations''' <br />
::::::: - from the O2k-Network »[[CZ Prague Krajcova A]]« <br />
::::::* Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - [[Krajcova 2020 PLOS ONE |»Bioblast link«]]<br />
<br />
2020 Week 2<br />
<br />
*'''Jan-07 Tu''' The Oroboros Office opens in the New Year 2020<br />
[[File:EBEClogo2022.jpg|right|300px|right|link=EBEC22 Innsbruck AT]]<br />
::::* From the '''Austrian Bioenergetics Group''' to '''Mitochondrial Physiology and Bioenergetics DACH''' (D: Germany; A: Austria; CH: Switzerland) <br />
:::::: [[EBEC22 Innsbruck AT |EBEC22 - the 22nd European Bioenergetics Conference]] will be held in Innsbruck (2022, Aug 22-27). We would like to invite several Austrian colleagues to join the ''EBEC22 National Scientific Committee''. This provides an excellent opportunity to establish an ''Austrian Bioenergetics Group'' towards and beyond EBEC22. We hope that this topic will be discussed at the forthcoming [[1st Myocardial Function Symposium 2020 Graz AT]], and further at the [[MitoEAGLE Summit Obergurgl 2020]]. Please, contact the organizer of EBEC22 - erich.gnaiger@oroboros.at<br />
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* '''Jan-08 We'''<br />
:::: [[File:MitoPedia.jpg|60px|MitoPedia]] '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
<br />
:::: [[Image:Quality.png|left|60px|link=http://wiki.oroboros.at/index.php/MitoPedia:_Oroboros_QM |Quality]] <br />
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* '''Jan-09 Th'''<br />
[[File:MITOEAGLE-representation.jpg|right|200px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]]<br />
::::* Planning the [[MitoEAGLE Summit Obergurgl 2020]]<br />
:::::: Members of the [[Management Committee MitoEAGLE]] and [[MitoEAGLE Working Groups |Working Group Leaders]]: please submit your suggestions for [[MitoEAGLE Summit Obergurgl 2020]]:<br />
::::::# Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output<br />
::::::# Suggested scientists (titles) for presentations, according to the MoU<br />
<br />
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* '''Jan-10 Fr''' <br />
::::» <big>'''[[1st Myocardial Function Symposium 2020 Graz AT]]'''</big> (Jan 10-11)<br />
::::* 14:45 Novel technologies for mitochondria and cell research (Gnaiger, Innsbruck, AUT)<br />
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* '''Jan-12 So'''<br />
::::* One month ago<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:: '''Cluster Awards 2019'''<br />
<br />
<hr><br />
<br />
{|<br />
|[[File:Cluster Awards 2019.jpg|right|500px|Cluster Awards 2019]]<br />
On December 12<sup>th</sup>, the '''Cluster Awards 2019''' were presented for the best Tyrolean innovations of the year. We are thrilled to be the recipient in the '''Life Sciences Cluster''' for our EU Horizon 2020-funded '''[[NextGen-O2k |NextGen-O2k project]]'''.<br />
<br />
'''Life Sciences'''<br />
::: Oroboros Instruments GmbH, Innsbruck: "NextGen-O2k"<br />
<br />
'''Renewable Energies'''<br />
::: Heliotherm Wärmepumpentechnik GmbH, Langkampfen: "Invisible Heatpump"<br />
<br />
'''Forest and wood'''<br />
::: Holzbau Unterrainer GmbH, Ainet: "Radius wood - production of curved cross laminated timber elements"<br />
<br />
'''Information Technologies'''<br />
::: Lo.La Peak Solutions GmbH, Trins: "LO.LA Safety - Digital Safety Management in Ski Resorts"<br />
<br />
'''Mechatronics''': ALPEX Technologies GmbH, Mils: "EVEREST 4.0"<br />
<br />
'''Wellness''': Organoid Technologies GmbH, Flow: "natural sauna surfaces made of hay"<br />
<br />
» More details (in German): '''[https://www.standort-tirol.at/page.cfm?vpath=newsroom/news&genericpageid=26328 Standortagentur Tirol]'''.<br />
<div style="text-align: right; direction: ltr; margin-left: 1em;">Photo © Standortagentur Tirol</div><br />
|}<br />
|}<br />
<br />
2020 Week 1 '''The Oroboros team enjoys ''holidays'' from 2019 Dec 24 to 2020 Jan 06'''<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: #white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
<br />
[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:'''[[Oroboros picture of the month]]'''<br />
<br />
<hr><br />
{|<br />
| <br />
[[File:Oroboros Winter.jpg|250px|Oroboros of the month]]<br />
| ''' Season's Greetings'''<br />
<br />
: Our office is closed until 2020-01-06. <br />
<br />
: We wish you a Happy New Year 2020! <br />
<br />
: Your Oroboros team<br />
::::::* the [[Oroboros_contact#Oroboros_Customer_Relations_Ninjas |Oroboros Customer Relations Ninjas]]<br />
::::::* the [[Oroboros_contact#Oroboros_Sales_Athletes |Oroboros Sales Athletes]]<br />
::::::* the [[Oroboros_contact#Oroboros_Bioenergetics_Adventurers |Oroboros Bioenergetics Adventurers]]<br />
::::::* the [[Oroboros_contact#Oroboros_Quality_Management_Angels |Oroboros Quality Management Angels]]<br />
::::::* the [[Oroboros_contact#Oroboros_Leadership_Group |Oroboros Leadership Group]]<br />
<br />
|}<br />
|}<br />
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*'''Jan-04 Sa'''<br />
:::: [[Image:O2k-News icon.jpg|60px|link=https://wiki.oroboros.at/index.php/Oroboros_News | News]] The [[Oroboros Ecosystem Agenda 2020]] is launched as a signature of the [[Oroboros Ecosystem]].<br />
<br />
:::: [[Image:Gentle-Science_Ganesha.jpg|60px|link=http://www.bioblast.at/index.php/Gentle_Science|Gentle Science]] [https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(19)33170-8/fulltext?dgcid=raven_jbs_etoc_email 2020: a critical year for women, gender equity, and health - a Lancet Editorial] <br />
<br />
::::* One month ago<br />
{| style="width:95%;<br />
|<div style="display: table; style="width: 95%; border-radius: 6px; background-color: white; border: 1px solid #090982; margin: 0.5em 1em 0 1em; padding: 6px; box-shadow: 0px 0px 5px #090982;><br />
<table><br />
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[[Image:Logo_OROBOROS_INSTRUMENTS.jpg|right|35px|link=http://wiki.oroboros.at/index.php/OROBOROS_INSTRUMENTS |Oroboros]]<br />
:: '''NextGen-O2k SME2 coaching Orily Pratt part 2'''<br />
<br />
<hr><br />
<br />
{|<br />
|[[File:2019-12-04 Coaching with Orily Pratt.jpg|left|250px|MitoFit Guest Seminar]] On '''December 03/04''' the second part of the '''SME phase 2 Coaching for the [[NextGen-O2k]] project''' with '''Orily Pratt''' (Tel Aviv, IL) took place in Innsbruck. <br />
<br/><br />
<br/><br />
<br />
:» Wednesday, 4th of December; Oroboros Instruments, Schoepfstrasse 18, MiPArt, Innsbruck, Austria.<br />
<br />
|}<br />
|}<br />
<br />
<br />
== Stay alert ==<br />
{{Oroboros Ecosystem Agenda list}}</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPedia:_DatLab&diff=205832
MitoPedia: DatLab
2020-08-04T15:24:20Z
<p>Antunes Diana: </p>
<hr />
<div>{{Technical support}}<br />
::: '''Welcome at MitoPedia: DatLab'''. Here you find [[O2k-Open Support|'''Oroboros support''']] for DatLab and the '''[[MitoPedia: O2k hardware |O2k]]'''.<br />
<br />
<br />
<big><big>'''List of DatLab menus'''</big></big><br />
__TOC__<br />
<br />
[[File:DatLab-bar.jpg|460px|link=DatLab]]<br />
[[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]<br />
<big><big>'''[[DatLab installation]]'''</big></big><br />
::::» <big> [[MiPNet21.16 DatLab 7 Innovations]]</big><br />
<br />
[[File:File-menu.png|right|150px]]<br />
=== File ===<br />
::::» [[Open DLD file|Open]]<br />
::::» [[Close - DatLab| Close]]<br />
::::» [[Save - DatLab | Save]]<br />
::::» [[Save and Disconnect | Save and disconnect]]<br />
::::» [[Save as - DatLab | Save as]]<br />
::::» [[File search - DatLab| File search]]<br />
::::» [[Delete - DatLab | Delete]]<br />
::::» Import DatLab templates [[DatLab templates]]<br />
::::» Export<br />
:::::* [[Export DL-Protocol User (*.DLPU)]]<br />
:::::* Data to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - Events to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - One channel to DatLab 2 analysis: [[DatLab 2]]<br />
::::» Change user: [[User code - DatLab | User]]<br />
::::» Manage users: [[User code - DatLab | User]]<br />
<br />
[[File:O2k-menu.png|right|150px]]<br />
=== Oroboros O2k ===<br />
::::» [[O2k configuration]]<br />
::::» [[O2k channel labels]]<br />
::::» [[O2k control]]<br />
::::» [[Stirrer A on/off]]<br />
::::» [[Stirrer B on/off]]<br />
::::» [[Stirrer test]]<br />
::::» [[Illumination on/off]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:TIP2k-menu.png|right|150px]]<br />
=== TIP2k ===<br />
::::» TIP2k control: [[MiPNet12.10 TIP2k-manual|Titration-Injection microPump]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:Protocol-menu.png|right|200px]]<br />
=== Protocol ===<br />
<br />
::::» [[Run DL-Protocol/Set O2 limit]]<br />
::::» [[Instrumental: Browse DL-Protocols and templates]]<br />
::::» [[SUIT: Browse DL-Protocols and templates]]<br />
::::» [[Install Oroboros protocol package]]<br />
::::» [[MitoPedia: SUIT |Browse www.bioblast.at/index.php./MitoPedia: SUIT]]<br />
::::» [[Enable DL-Protocol editing]]<br />
<br />
[[File:Experiment-menu.png|right|150px]]<br />
=== Experiment ===<br />
::::» Sample: [[Sample - DatLab |Sample]]<br />
::::» Exp. log: [[Experimental log - DatLab |Experimental log]]<br />
::::» Add event: [[Events - DatLab |Events]]<br />
<br />
[[File:Calibration-menu.png|right|150px]]<br />
=== Calibration ===<br />
::::» O2: [[O2 calibration - DatLab|O2 calibration]]<br />
::::» Amp: [[Amp calibration - DatLab|Amp calibration]] <br />
::::» pX: [[PX calibration - DatLab|pX calibration]]<br />
<br />
[[File:Flux-Slope-menu.png|right|130px]]<br />
=== Flux / Slope ===<br />
::::» O2: [[Flux / Slope]]<br />
::::» Amp: [[Flux / Slope]]<br />
::::» pX: [[Flux / Slope]]<br />
::::» Proton [[Flux / Slope]]<br />
<br />
[[File:Graph-menu.png|right|150px]]<br />
=== Graph ===<br />
::::» Add graph/Delete bottom graph: [[Add Graph/Delete bottom graph|Add/Delete]]<br />
::::» Select plots: [[Select plots - DatLab]]<br />
::::» Scaling: [[Scaling - DatLab]]<br />
::::» Info: [[Layout_for_DatLab_graphs#Graph / Info|Graph / Info]]<br />
::::» [[Synchronous time axes]]<br />
::::» [[Autoscale]]<br />
:::::* [[Autoscale time axis]]<br />
:::::* [[Autoscale Y1 (Y2) axes]]<br />
:::::* [[Automatic pan]]<br />
::::» Mouse control: [[Mouse control: Mark|Mark]] and [[Mouse control: Zoom|Zoom]]<br />
::::» [[Full screen]]<br />
::::» [[Display numerical value]]<br />
::::» [[Display Power-O2k]]<br />
::::» Options: [[Graph options - DatLab |Graph options]]<br />
::::» [[Copy to clipboard]]<br />
<br />
<br />
[[File:Layout-menu.png|right|150px]]<br />
=== Layout ===<br />
::::» Info / Load / Save: [[Layout_for_DatLab_graphs#Layout: Info/ Load/ Safe| Layout: Info/ Load/ Safe]]<br />
::::» Standard layouts - O2k-FluoRespirometer: [[Layout_for_DatLab_graphs#Standard layouts - O2k-FluoRespirometer |Standard layouts - O2k-FluoRespirometer]]<br />
::::» Lab layouts: [[Layout_for_DatLab_graphs#Lab layouts| Lab layouts]]<br />
::::» User: Name: [[Layout_for_DatLab_graphs#User: Name| User: Name]]<br />
<br />
[[File:Marks-menu.png|right|150px]]<br />
=== Marks ===<br />
::::» Edit mark information: [[Marks - DatLab|Marks]]<br />
::::» Statistics: [[Mark statistics - DatLab|Mark statistics]]<br />
::::» Manage mark stat. templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» Specifications: [[Mark specifications - DatLab |Mark specifications]]<br />
::::» Manage mark specifications templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» [[Copy marks]]<br />
<br />
<br />
== DatLab glossary ==<br />
<br />
{{#ask:[[MitoPedia O2k and high-resolution respirometry::DatLab]]<br />
| mainlabel=Term<br />
|?Has abbr=Abbreviation<br />
|?Description=Description<br />
|format=broadtable<br />
|limit=1000<br />
|order=ascending<br />
}}<br />
<br />
<br />
<br />
== Troubleshooting ==<br />
<br />
=== Problems with text in DatLab ===<br />
: Values, symbols, and/or units may appear incorrectly in the DatLab software (''e.g.,'' ''J''°1 mark name, events/marks names)<br><br />
:'''Example:'''<br><br />
<br><br />
:[[File:DatLab problem 2020-02-21DL.png|700px]]<br />
<br><br />
:'''Solution:'''<br><br />
: It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings and selecting a language option such as English (United Kingdom) or English (United States). Follow the steps below:<br />
<br><br />
:[[File:DatLab problem 2020-02-19-01.jpeg|800px]]<br />
<br />
<br />
<br />
== Further links ==<br />
<br />
::::» [[O2k-Manual#DatLab_manuals |O2k-Manual: DatLab]]<br />
::::» [[O2k-Demo experiments]]<br />
::::» [[DatLab |O2k-Catalogue: DatLab]]<br />
<br />
<br />
::: Related topics<br />
::::» [[MitoPedia: Respirometry|Respirometry]]<br />
::::» [[MitoPedia: Fluorometry|Fluorometry]]<br />
::::» [[MitoPedia: Spectrophotometry|Spectrophotometry]]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Current_week_ecosystem_agenda&diff=205831
Template:Current week ecosystem agenda
2020-08-04T15:16:05Z
<p>Antunes Diana: </p>
<hr />
<div> 2020 Week 32<br />
* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br><br />
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]«<br />
|}<br />
----<br />
* '''Aug-04 Tu'''<br />
<br />
:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br><br />
:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]« <br />
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]<br />
|}<br />
<br />
----<br />
* '''Aug-05 We'''<br />
<br />
----<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|} <br />
----<br />
* '''Aug-07 Fr'''</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=O2k-OpenSupportAlert2020_Archive&diff=205830
O2k-OpenSupportAlert2020 Archive
2020-08-04T15:15:33Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
<br />
<br />
== Aug 2020 ==<br />
* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if values, symbols, and/or units are not correctly displayed in the DatLab software?'''<br><br />
It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_DatLab| Troubleshooting for problems with text in DatLab]]«<br />
|}<br />
== Previous O2k-Open Support Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
<br />
<br />
== Jul 2020 ==<br />
<br />
* '''Jul-27 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you know how to prepare a homogenate from tissue samples using the [https://www.oroboros.at/index.php/product/pbi-shredder-o2k-set/ PBI-Shredder O2k-Set]?'''<br />
The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility and quality. <br><br />
- » [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]«<br />
|} <br />
----<br />
<br />
* '''Jul-20 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''How can you compare respirometric experiments using an internal normalization?'''<br><br />
Flux control ratios (''FCR'') are independent of external measurements (''e.g.'' protein determination, citrate synthase activity) and therefore can be used to analyze data when other normalization methods cannot be applied. Learn more about how to use this option to assess qualitative changes of mitochondrial respiratory control with DatLab and the “O2 analysis template DL7.4”. <br />
<br><br />
- »[[Flux control ratio]]«<br />
<br />
Read more: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
----<br />
* '''Jul-13 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
|'''Did you know that you can apply instrumental O<sub>2</sub> background test parameters (''i.e.'', intercept and slope) from an existing file in DatLab?''' <br><br />
In the DatLab file, you can copy the Instrumental O<sub>2</sub> background test parameters directly via the ‘Copy from file’ option in the ‘Slope configuration’ window. <br />
For more information, see:<br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Instrumental_O2_DLP Copy from file]«<br />
|}<br />
----<br />
<br />
* '''Jul-06 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Do you know what to do when the O2k-Peltier takes longer than 20 minutes to reach the set temperature? ''' <br><br />
During [[Air calibration | air calibration]], temperature of the O2k-chamber and the O2k-Peltier power can be monitored under the layout '01 Calibration show Temp'. In case of an issue with the [[O2k-Peltier Temperature Control| temperature control]], an error message will appear. <br />
For more information, see:<br />
- »[[O2k-Peltier_Mitopedia#Block_temperature_does_not_increase_while_heating_with_full_power|Block temperature does not increase while heating with full power]]« and »[[DatLab error messages]] «<br />
|}<br />
<br />
== Jun 2020 ==<br />
<br />
* '''Jun-01 Mo''' <br />
:{|<br />
| width="110" align="center" |[[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that instabilities of the slope of the oxygen signal (O<sub>2</sub> slope neg.) can be prevented by the proper use of TIP2k-Filter Papers during an instrumental O<sub>2</sub> background test?'''<br><br />
- »[https://wiki.oroboros.at/index.php/Oxygen_flux_-_instrumental_background#Unstable_O2_slope_neg._in_instrumental_background_test Unstable 'O2 slope neg.']« and »[[TIP2k Filter Papers]]«<br />
|}<br />
----<br />
* '''Jun-15 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
|'''Did you know that experimental artefacts can occur in the Amplex UltraRed assay after injection of chemicals that interfere with the fluorescence signal?'''<br />
- » [https://wiki.oroboros.at/index.php/Amplex_UltraRed#Injection_artefact Injection artefact]«<br />
|}<br />
<br />
----<br />
<br />
* '''Jun-22 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Did you know a stirrer test can save your experiments?'''<br><br />
Get immediate information on how satisfactory your OroboPOS response time is by performing a [[Stirrer_test|stirrer test]] during [[Air calibration | air calibration]].<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
* '''Jun-29 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Is the noise seen in the slope of the oxygen signal (O2 slope neg.) within recommended values?'''<br><br />
:An 'O2 slope neg.' within ± 2 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is optimal (± 4 pmol∙s<sup>−1</sup>∙mL<sup>−1</sup> is acceptable) at a data recording interval of 2 s and 40 data points selected for calculation of the slope.<br><br />
- »[[Oxygen_calibration_-_DatLab#Instrumental_DL-Protocols| Air and zero calibration]]«<br />
|}<br />
=== May 2020 ===<br />
<br />
* '''May-25 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you know how the "switch components" approach can help you find what is causing your O2k to not reach the quality control specifications?'''<br><br />
Do not despair if specifications given in the O2k-Manuals are not obtained. See how to troubleshoot on your own.<br><br />
- »[[Oxygen_sensor_test#Switch_components_approach|Switch components approach]]«<br />
|}<br />
<br />
* '''May-18 Mo''' <br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]] <br />
|'''Do you know that a humid O2k-environment and/or liquid spillage may lead to a high raw POS signal [V]?'''<br><br />
See how to clean and dry the electrical connections of the OroboPOS-Connector.<br><br />
- »[[OroboPOS-Connector#Cleaning_the_electrical_connections|Cleaning the electrical connections]]«<br />
|}<br />
<br />
* '''May-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|''' Do you know how to avoid underestimating/overestimating respiratory fluxes? Measuring flux at steady states with high-resolution respirometry is the key to obtaining correct values.''' <br><br />
Learn more about steady states including performance and mark setting for data analysis. <br><br />
- »[[Steady state]]«<br />
|}<br />
<br><br />
* '''May-04 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you load the correct instrumental background correction according to the actual of experimental O<sub>2</sub> concentrations?'''<br><br />
Perform and apply the instrumental background test.<br><br />
- »[[Flux / Slope| O2 background correction in DatLab]]« and »[[Oxygen_flux_-_instrumental_background | Instrumental oxygen background]]«<br />
|}<br />
=== Apr 2020 ===<br />
* '''Apr-27 Mo'''<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Do you set marks correctly in the instrumental O<sub>2</sub> background test when planning to apply the background correction to a [https://wiki.oroboros.at/index.php/MitoPedia:_SUIT SUIT protocol] with a high O<sub>2</sub> regime?'''<br><br />
The range of O<sub>2</sub> concentrations selected in the instrumental background test should correspond to the range of experimental O<sub>2</sub> concentrations.<br><br />
- »[[Oxygen_flux_-_instrumental_background#Linear_regressions_values_.28intercept_-_a.C2.B0_and_the_slope_-_b.C2.B0.29_are_very_different_than_the_expected_values | O2 Background correction - troubleshooting]]«<br />
|}<br />
<br />
<br><br />
* '''Apr-20 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Improve]]<br />
|'''Did you know that some substrates alone cannot maintain a stable mitochondrial respiratory flux in OXPHOS?'''<br><br />
Unstable respiratory fluxes in OXPHOS might be due to substrates limitation.<br><br />
- »[[Pyruvate#Troubleshooting | Pyruvate - troubleshooting]]«<br />
|}<br />
<br><br />
* '''Apr-14 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
|'''Why are the oxygen levels different between chambers prior to my experimental run?'''<br><br />
After switching on the O2k, air calibration has to be performed; otherwise the displayed concentrations may deviate from accurate concentrations.<br><br />
- »[[Oxygen_calibration_-_DatLab#Troubleshooting | Oxygen calibration - troubleshooting]]«<br />
|}<br />
<br><br />
* '''Apr-07 Tu'''<br />
:{|<br />
| width="110" align="center" | [[Image:Logo OROBOROS-DatLab.jpg|70px|link=http://wiki.oroboros.at/index.php/DatLab |DatLab]][[File:Blue-info.png|25px|Improve]]<br />
|'''Did you know that you can edit the background parameters in the 'O<sub>2</sub> analysis template DL7.4?'''<br><br />
If you did not apply a finally evaluated O2 background correction to your experimental DatLab file, you can edit the O2 background parameters directly in the '''O2 analysis template DL7.4''' for completion of [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4 |Oxygen flux analysis - DatLab 7.4]].<br><br />
- »[[SUIT: Browse DL-Protocols and templates]]«<br />
|}<br />
<br><br />
* '''Apr-02 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|70px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]][[File:Blue-info.png|25px|Improve]]<br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?'''<br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-30 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''DatLab 7.4 offers the flexibility to edit a DL-Protocol:'''<br><br />
- »'''[[Enable DL-Protocol editing]]'''«<br />
|}<br />
<br><br />
* '''Mar-25 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''O<sub>2</sub> flux analysis with DatLab 7.4'''. Use our latest O<sub>2</sub> analysis template DL7.4:<br><br />
- »'''[https://www.youtube.com/watch?v=HRAGvmaK01I| O2k-Videosupport: O<sub>2</sub> flux analysis with DatLab 7.4]'''«<br />
|}<br />
<br><br />
* '''Mar-23 Mo'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you know that you can use the [[TIP2k-Module|Titration-Injection microPump (TIP2k)]] to perform an instrumental O<sub>2</sub> background test when using the [[O2k-sV-Module]]?'''<br><br />
'''(1) By adjusting the dithionite concentration to 2.5 mM''', the instrumental O<sub>2</sub> background test can be performed with the "BG Feedback" settings. - »'''[[Dithionite|Dithionite]]'''« <br><br />
'''(2) By adjusting the TIP2k setup with specific settings for the O2k-sV-Module''', the instrumental O<sub>2</sub> background test can be performed using the O2k-sV-Module. - »'''[[MiPNet24.14 O2k-sV-Module manual|O2k-sV-Module Manual]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you change the [[Smoothing|slope smoothing]] to 20 while running experiments with biological sample?'''<br />
While running your experiment, especially during [[uncoupler titrations]], it is important to decrease the slope smoothing in order to have a faster visualization of the titrations effect on respiration.<br><br />
- »[[Smoothing#DatLab_oxygen_flux:_performance_and_data_analysis|Slope smoothing]]«<br />
|}<br />
<br><br />
* '''Mar-04 We'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
| [[File:OroboPOS-Holder002.jpg|100px]]<br />
| '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''<br />
See how you can check that the two blue OroboPOS-Holders are intact.<br><br />
- »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-28 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Calibration parameters are transferred to experimental DatLab files'''<br />
Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.<br><br />
- »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
=== Jan 2020 ===<br />
<br />
* '''Jan-31 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''<br />
In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP).<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br><br />
* '''Jan-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''<br />
In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample.<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you set mark ‘R1’ and complete the air calibration correctly?'''<br />
Your air calibration is not effective until you set mark ‘R1’ and click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window.<br><br />
- »[[Oxygen calibration - DatLab]]«<br />
|}<br />
<br><br />
*'''Jan-24 Fr'''<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''<br />
Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed.<br><br />
- »[[O2k configuration]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Red-loss of data.png|25px|Warning: loss of data]]<br />
| '''Do you perform O2k-Chamber volume calibration?'''<br />
The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]).<br><br />
- »[[O2k-Chamber MitoPedia]]«<br />
|}<br />
<br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Take care]]<br />
| '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?'''<br />
The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration.<br><br />
- »[[Oxygen solubility factor]]«<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPedia:_DatLab&diff=205829
MitoPedia: DatLab
2020-08-04T15:10:29Z
<p>Antunes Diana: </p>
<hr />
<div>{{Technical support}}<br />
::: '''Welcome at MitoPedia: DatLab'''. Here you find [[O2k-Open Support|'''Oroboros support''']] for DatLab and the '''[[MitoPedia: O2k hardware |O2k]]'''.<br />
<br />
<br />
<big><big>'''List of DatLab menus'''</big></big><br />
__TOC__<br />
<br />
[[File:DatLab-bar.jpg|460px|link=DatLab]]<br />
[[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]<br />
<big><big>'''[[DatLab installation]]'''</big></big><br />
::::» <big> [[MiPNet21.16 DatLab 7 Innovations]]</big><br />
<br />
[[File:File-menu.png|right|150px]]<br />
=== File ===<br />
::::» [[Open DLD file|Open]]<br />
::::» [[Close - DatLab| Close]]<br />
::::» [[Save - DatLab | Save]]<br />
::::» [[Save and Disconnect | Save and disconnect]]<br />
::::» [[Save as - DatLab | Save as]]<br />
::::» [[File search - DatLab| File search]]<br />
::::» [[Delete - DatLab | Delete]]<br />
::::» Import DatLab templates [[DatLab templates]]<br />
::::» Export<br />
:::::* [[Export DL-Protocol User (*.DLPU)]]<br />
:::::* Data to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - Events to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - One channel to DatLab 2 analysis: [[DatLab 2]]<br />
::::» Change user: [[User code - DatLab | User]]<br />
::::» Manage users: [[User code - DatLab | User]]<br />
<br />
[[File:O2k-menu.png|right|150px]]<br />
=== Oroboros O2k ===<br />
::::» [[O2k configuration]]<br />
::::» [[O2k channel labels]]<br />
::::» [[O2k control]]<br />
::::» [[Stirrer A on/off]]<br />
::::» [[Stirrer B on/off]]<br />
::::» [[Stirrer test]]<br />
::::» [[Illumination on/off]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:TIP2k-menu.png|right|150px]]<br />
=== TIP2k ===<br />
::::» TIP2k control: [[MiPNet12.10 TIP2k-manual|Titration-Injection microPump]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:Protocol-menu.png|right|200px]]<br />
=== Protocol ===<br />
<br />
::::» [[Run DL-Protocol/Set O2 limit]]<br />
::::» [[Instrumental: Browse DL-Protocols and templates]]<br />
::::» [[SUIT: Browse DL-Protocols and templates]]<br />
::::» [[Install Oroboros protocol package]]<br />
::::» [[MitoPedia: SUIT |Browse www.bioblast.at/index.php./MitoPedia: SUIT]]<br />
::::» [[Enable DL-Protocol editing]]<br />
<br />
[[File:Experiment-menu.png|right|150px]]<br />
=== Experiment ===<br />
::::» Sample: [[Sample - DatLab |Sample]]<br />
::::» Exp. log: [[Experimental log - DatLab |Experimental log]]<br />
::::» Add event: [[Events - DatLab |Events]]<br />
<br />
[[File:Calibration-menu.png|right|150px]]<br />
=== Calibration ===<br />
::::» O2: [[O2 calibration - DatLab|O2 calibration]]<br />
::::» Amp: [[Amp calibration - DatLab|Amp calibration]] <br />
::::» pX: [[PX calibration - DatLab|pX calibration]]<br />
<br />
[[File:Flux-Slope-menu.png|right|130px]]<br />
=== Flux / Slope ===<br />
::::» O2: [[Flux / Slope]]<br />
::::» Amp: [[Flux / Slope]]<br />
::::» pX: [[Flux / Slope]]<br />
::::» Proton [[Flux / Slope]]<br />
<br />
[[File:Graph-menu.png|right|150px]]<br />
=== Graph ===<br />
::::» Add graph/Delete bottom graph: [[Add Graph/Delete bottom graph|Add/Delete]]<br />
::::» Select plots: [[Select plots - DatLab]]<br />
::::» Scaling: [[Scaling - DatLab]]<br />
::::» Info: [[Layout_for_DatLab_graphs#Graph / Info|Graph / Info]]<br />
::::» [[Synchronous time axes]]<br />
::::» [[Autoscale]]<br />
:::::* [[Autoscale time axis]]<br />
:::::* [[Autoscale Y1 (Y2) axes]]<br />
:::::* [[Automatic pan]]<br />
::::» Mouse control: [[Mouse control: Mark|Mark]] and [[Mouse control: Zoom|Zoom]]<br />
::::» [[Full screen]]<br />
::::» [[Display numerical value]]<br />
::::» [[Display Power-O2k]]<br />
::::» Options: [[Graph options - DatLab |Graph options]]<br />
::::» [[Copy to clipboard]]<br />
<br />
<br />
[[File:Layout-menu.png|right|150px]]<br />
=== Layout ===<br />
::::» Info / Load / Save: [[Layout_for_DatLab_graphs#Layout: Info/ Load/ Safe| Layout: Info/ Load/ Safe]]<br />
::::» Standard layouts - O2k-FluoRespirometer: [[Layout_for_DatLab_graphs#Standard layouts - O2k-FluoRespirometer |Standard layouts - O2k-FluoRespirometer]]<br />
::::» Lab layouts: [[Layout_for_DatLab_graphs#Lab layouts| Lab layouts]]<br />
::::» User: Name: [[Layout_for_DatLab_graphs#User: Name| User: Name]]<br />
<br />
[[File:Marks-menu.png|right|150px]]<br />
=== Marks ===<br />
::::» Edit mark information: [[Marks - DatLab|Marks]]<br />
::::» Statistics: [[Mark statistics - DatLab|Mark statistics]]<br />
::::» Manage mark stat. templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» Specifications: [[Mark specifications - DatLab |Mark specifications]]<br />
::::» Manage mark specifications templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» [[Copy marks]]<br />
<br />
<br />
== DatLab glossary ==<br />
<br />
{{#ask:[[MitoPedia O2k and high-resolution respirometry::DatLab]]<br />
| mainlabel=Term<br />
|?Has abbr=Abbreviation<br />
|?Description=Description<br />
|format=broadtable<br />
|limit=1000<br />
|order=ascending<br />
}}<br />
<br />
<br />
<br />
== Troubleshooting ==<br />
<br />
=== Problems with text in DatLab ===<br />
: Values, symbols, and/or units may appear incorrectly in the DatLab software (''e.g.,'' ''J''°1 mark name, events/marks names)<br><br />
:'''Example:'''<br><br />
<br><br />
:[[File:DatLab problem 2020-02-21DL.png|700px]]<br />
<br><br />
: It is possible to retrieve the values, symbols and/or correct units by updating the Windows control panel settings and selecting a language option such as English (United Kingdom) or English (United States). Follow the steps below:<br />
<br><br />
:[[File:DatLab problem 2020-02-19-01.jpeg|800px]]<br />
<br />
<br />
<br />
== Further links ==<br />
<br />
::::» [[O2k-Manual#DatLab_manuals |O2k-Manual: DatLab]]<br />
::::» [[O2k-Demo experiments]]<br />
::::» [[DatLab |O2k-Catalogue: DatLab]]<br />
<br />
<br />
::: Related topics<br />
::::» [[MitoPedia: Respirometry|Respirometry]]<br />
::::» [[MitoPedia: Fluorometry|Fluorometry]]<br />
::::» [[MitoPedia: Spectrophotometry|Spectrophotometry]]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=File:DatLab_problem_2020-02-21DL.png&diff=205828
File:DatLab problem 2020-02-21DL.png
2020-08-04T15:07:40Z
<p>Antunes Diana: Antunes Diana uploaded a new version of File:DatLab problem 2020-02-21DL.png</p>
<hr />
<div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPedia:_DatLab&diff=205827
MitoPedia: DatLab
2020-08-04T14:31:22Z
<p>Antunes Diana: </p>
<hr />
<div>{{Technical support}}<br />
::: '''Welcome at MitoPedia: DatLab'''. Here you find [[O2k-Open Support|'''Oroboros support''']] for DatLab and the '''[[MitoPedia: O2k hardware |O2k]]'''.<br />
<br />
<br />
<big><big>'''List of DatLab menus'''</big></big><br />
__TOC__<br />
<br />
[[File:DatLab-bar.jpg|460px|link=DatLab]]<br />
[[Image:Logo OROBOROS-DatLab.jpg|left|60px||link=http://wiki.oroboros.at/index.php/DatLab |DatLab]]<br />
<big><big>'''[[DatLab installation]]'''</big></big><br />
::::» <big> [[MiPNet21.16 DatLab 7 Innovations]]</big><br />
<br />
[[File:File-menu.png|right|150px]]<br />
=== File ===<br />
::::» [[Open DLD file|Open]]<br />
::::» [[Close - DatLab| Close]]<br />
::::» [[Save - DatLab | Save]]<br />
::::» [[Save and Disconnect | Save and disconnect]]<br />
::::» [[Save as - DatLab | Save as]]<br />
::::» [[File search - DatLab| File search]]<br />
::::» [[Delete - DatLab | Delete]]<br />
::::» Import DatLab templates [[DatLab templates]]<br />
::::» Export<br />
:::::* [[Export DL-Protocol User (*.DLPU)]]<br />
:::::* Data to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - Events to text file: [[Text file - DatLab | Text file]]<br />
:::::* Export - One channel to DatLab 2 analysis: [[DatLab 2]]<br />
::::» Change user: [[User code - DatLab | User]]<br />
::::» Manage users: [[User code - DatLab | User]]<br />
<br />
[[File:O2k-menu.png|right|150px]]<br />
=== Oroboros O2k ===<br />
::::» [[O2k configuration]]<br />
::::» [[O2k channel labels]]<br />
::::» [[O2k control]]<br />
::::» [[Stirrer A on/off]]<br />
::::» [[Stirrer B on/off]]<br />
::::» [[Stirrer test]]<br />
::::» [[Illumination on/off]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:TIP2k-menu.png|right|150px]]<br />
=== TIP2k ===<br />
::::» TIP2k control: [[MiPNet12.10 TIP2k-manual|Titration-Injection microPump]]<br />
::::» Manage setups: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
<br />
[[File:Protocol-menu.png|right|200px]]<br />
=== Protocol ===<br />
<br />
::::» [[Run DL-Protocol/Set O2 limit]]<br />
::::» [[Instrumental: Browse DL-Protocols and templates]]<br />
::::» [[SUIT: Browse DL-Protocols and templates]]<br />
::::» [[Install Oroboros protocol package]]<br />
::::» [[MitoPedia: SUIT |Browse www.bioblast.at/index.php./MitoPedia: SUIT]]<br />
::::» [[Enable DL-Protocol editing]]<br />
<br />
[[File:Experiment-menu.png|right|150px]]<br />
=== Experiment ===<br />
::::» Sample: [[Sample - DatLab |Sample]]<br />
::::» Exp. log: [[Experimental log - DatLab |Experimental log]]<br />
::::» Add event: [[Events - DatLab |Events]]<br />
<br />
[[File:Calibration-menu.png|right|150px]]<br />
=== Calibration ===<br />
::::» O2: [[O2 calibration - DatLab|O2 calibration]]<br />
::::» Amp: [[Amp calibration - DatLab|Amp calibration]] <br />
::::» pX: [[PX calibration - DatLab|pX calibration]]<br />
<br />
[[File:Flux-Slope-menu.png|right|130px]]<br />
=== Flux / Slope ===<br />
::::» O2: [[Flux / Slope]]<br />
::::» Amp: [[Flux / Slope]]<br />
::::» pX: [[Flux / Slope]]<br />
::::» Proton [[Flux / Slope]]<br />
<br />
[[File:Graph-menu.png|right|150px]]<br />
=== Graph ===<br />
::::» Add graph/Delete bottom graph: [[Add Graph/Delete bottom graph|Add/Delete]]<br />
::::» Select plots: [[Select plots - DatLab]]<br />
::::» Scaling: [[Scaling - DatLab]]<br />
::::» Info: [[Layout_for_DatLab_graphs#Graph / Info|Graph / Info]]<br />
::::» [[Synchronous time axes]]<br />
::::» [[Autoscale]]<br />
:::::* [[Autoscale time axis]]<br />
:::::* [[Autoscale Y1 (Y2) axes]]<br />
:::::* [[Automatic pan]]<br />
::::» Mouse control: [[Mouse control: Mark|Mark]] and [[Mouse control: Zoom|Zoom]]<br />
::::» [[Full screen]]<br />
::::» [[Display numerical value]]<br />
::::» [[Display Power-O2k]]<br />
::::» Options: [[Graph options - DatLab |Graph options]]<br />
::::» [[Copy to clipboard]]<br />
<br />
<br />
[[File:Layout-menu.png|right|150px]]<br />
=== Layout ===<br />
::::» Info / Load / Save: [[Layout_for_DatLab_graphs#Layout: Info/ Load/ Safe| Layout: Info/ Load/ Safe]]<br />
::::» Standard layouts - O2k-FluoRespirometer: [[Layout_for_DatLab_graphs#Standard layouts - O2k-FluoRespirometer |Standard layouts - O2k-FluoRespirometer]]<br />
::::» Lab layouts: [[Layout_for_DatLab_graphs#Lab layouts| Lab layouts]]<br />
::::» User: Name: [[Layout_for_DatLab_graphs#User: Name| User: Name]]<br />
<br />
[[File:Marks-menu.png|right|150px]]<br />
=== Marks ===<br />
::::» Edit mark information: [[Marks - DatLab|Marks]]<br />
::::» Statistics: [[Mark statistics - DatLab|Mark statistics]]<br />
::::» Manage mark stat. templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» Specifications: [[Mark specifications - DatLab |Mark specifications]]<br />
::::» Manage mark specifications templates: [[Manage setups and templates - DatLab |Manage setups and templates]]<br />
::::» [[Copy marks]]<br />
<br />
<br />
== DatLab glossary ==<br />
<br />
{{#ask:[[MitoPedia O2k and high-resolution respirometry::DatLab]]<br />
| mainlabel=Term<br />
|?Has abbr=Abbreviation<br />
|?Description=Description<br />
|format=broadtable<br />
|limit=1000<br />
|order=ascending<br />
}}<br />
<br />
<br />
<br />
== Troubleshooting ==<br />
<br />
=== Problems with text ===<br />
:''e.g.,'' J°1 mark name appears wrong or is not recognized by DatLab, or other events or marks names appear incorrectly.<br />
:Select a language option such as English (United Kingdom) or English (United States):<br />
<br />
:[[File:DatLab problem 2020-02-21-01.jpeg|400px]]<br />
<br />
=== Problems with text in the “Mark Statistics” window ===<br />
: ''e.g.,'' In the “Mark statistics” window, values for the “O2 slope background” fields do not appear or units are incorrect.<br />
:Example:<br><br />
:[[File:DatLab problem 2020-02-21DL.png|700px]]<br />
: It is possible to retrieve the values and correct units displayed in the “Mark statistics” window by updating the Windows control panel settings. Follow the steps below:<br />
:[[File:DatLab problem 2020-02-19-01.jpeg|800px]]<br />
<br />
<br />
<br />
== Further links ==<br />
<br />
::::» [[O2k-Manual#DatLab_manuals |O2k-Manual: DatLab]]<br />
::::» [[O2k-Demo experiments]]<br />
::::» [[DatLab |O2k-Catalogue: DatLab]]<br />
<br />
<br />
::: Related topics<br />
::::» [[MitoPedia: Respirometry|Respirometry]]<br />
::::» [[MitoPedia: Fluorometry|Fluorometry]]<br />
::::» [[MitoPedia: Spectrophotometry|Spectrophotometry]]</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=File:DatLab_problem_2020-02-21DL.png&diff=205826
File:DatLab problem 2020-02-21DL.png
2020-08-04T14:30:15Z
<p>Antunes Diana: </p>
<hr />
<div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=MitoPediaAlert2020_Archive&diff=205766
MitoPediaAlert2020 Archive
2020-08-03T06:30:58Z
<p>Antunes Diana: </p>
<hr />
<div>:::::: <big>''Currently Answered Questions from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«<br />
</big>__TOC__<br />
== Aug 2020 ==<br />
<br />
* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|}<br />
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== Previous MitoPedia Alert 2020 ==<br />
[[File:Expand.png|right|35px |Click to expand or collaps]]<br />
<div style="background-color:#f0f5ff;" class="toccolours mw-collapsible mw-expanded"><br />
<span style="font-size:105%"> >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>></span><br />
<div class="mw-collapsible-content"><br />
== Jul 2020 ==<br />
<br />
* '''Jul-30 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Chlororespiration is the phenomenon by which oxygen is consumed in the chloroplasts of plant cells:''' <br><br />
- »'''[[Chlororespiration]]'''«<br />
|}<br />
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* '''Jul-24 Fr''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to assess the integrity of the [[mitochondrial outer membrane]] in [[mitochondrial preparations]]? ''' <br />
It is possible to do so using exogenously added [[cytochrome c|cytochrome ''c'']]: <br><br />
- »'''[[Cytochrome_c_control_factor#Determination_of_cytochrome_c_loss|Cytochrome c control factor]]'''«<br />
|}<br />
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* '''Jul-16 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know how to select the proper Fluorescence-Sensor and filter set?''' <br><br />
Using different Fluorescence-Sensors and [[Filters]], the [https://www.oroboros.at/index.php/product/o2k-fluo-smart-module/ O2k-Fluo Smart-Module] allows for measurement of [[Hydrogen peroxide]], [[ATP]], [[Mitochondrial membrane potential]], [[Calcium]], and numerous other applications open for O2k-user innovation.<br><br />
- »'''[[O2k-Fluo_Smart-Module#Select_the_Smart_Fluo-Sensors | Selecting the Smart Fluo-Sensor]]'''« and »'''[[O2k-Fluo_LED2-Module#Setup_of_the_O2k-Fluo_LED2-Module | Setup of the O2k-Fluo LED2-Module]]'''«<br />
|}<br />
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* '''Jul-09 Th'''<br />
<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?''' <br><br />
- »'''[[Setting_the_oxygen_concentration |Setting the oxygen concentration]]'''«<br />
|}<br />
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* '''Jul-02 Th''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that if [[ET_capacity|ET capacity]] [[File:E.jpg]] is lower than [[OXPHOS_capacity|OXPHOS capacity]] [[File:P.jpg]] ([[ET_capacity#The_important_difference_between_states_P_and_E|''P''>''E'']]) in [[Living_cells | living cells]] or [[Mitochondrial_preparations | mitochondrial preparations]], then you have encountered an experimental artefact? The possible triggers of this experimental artefact are:''' <br><br />
- loss of oxidative capacity during the time course of the respirometric assay,<br><br />
- insufficient [[Uncoupler|uncoupler]] concentrations,<br> <br />
- over titration of an uncoupler, which inhibits ET capacity,<br><br />
- inhibition of ET capacity due to the use of high [[Oligomycin|oligomycin]] (inhibitor of the ATP synthase) concentrations employed to measure [[LEAK_respiration|LEAK respiration]] prior ET capacity.<br />
Information from: Gnaiger Erich ''et al'' ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »[[BEC_2020.1_doi10.26124bec2020-0001.v1|Bioblast link]]« <br />
|}<br />
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== Jun 2020 ==<br />
* '''Jun-25 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples?''' <br><br />
- » '''[https://wiki.oroboros.at/index.php/Dithionite Dithionite]'''«<br />
|}<br />
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* '''Jun-18 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Did you know the [[Crabtree effect]] can affect respiration in [[living cells]]?'''<br />
[[SUIT-003]] is designed to analyze this important mechanism of respiratory control.<br><br />
- »'''[[SUIT-003 O2 ce D037]]'''« and »'''[[SUIT-003 O2 ce D038]]'''«<br />
|}<br />
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* '''Jun-11 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that some chemicals are incompatible with the Amplex UltraRed assay? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Substances_incompatible_with_the_Amplex_Red_method Incompatible substrates]'''«<br />
|}<br />
<br />
* '''Jun-08 Mo''' <br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''Celebrating World Oceans Day'''<br><br />
<br />
Would you like to measure the O<sub>2</sub> flux that occurs during [[photosynthesis]] using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 [[NextGen-O2k]] project. <br><br />
- »'''[[PhotoBiology]]'''«<br />
|}<br />
<br />
* '''Jun-04 Th'''<br />
:{|<br />
| width="110" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Does your sample display a preference for [[GM|glutamate & malate]] over [[PM|pyruvate & malate]] to support NADH-linked respiration?''' <br><br />
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates. <br><br />
-»'''[[SUIT-008]]'''« and »'''[[SUIT-014]]'''«<br />
|}<br />
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=== May 2020 ===<br />
<br />
* '''May-28 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? <br><br />
- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Nitrogen_injection Nitrogen injection]'''«<br />
|}<br />
<br />
* '''May-21 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
|As part of the [[NextGen-O2k|H2020 SME NextGen-O2k]] project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O<sub>2</sub> consumption and production.<br><br />
-»'''[[PhotoBiology]]'''«<br />
|}<br />
* '''May-14 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|60px|MitoPedia]]<br />
|How can I measure '''ATP production''' with the O2k? <br><br />
Learn more about how to measure ATP production in [[mitochondrial preparations]] with Magnesium Green <br><br />
- »'''[[Magnesium Green]]'''«<br />
|}<br />
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* '''May-07 Th'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|Do you know how to evaluate '''[[Fatty acid oxidation pathway control state|fatty acid oxidation (FAO, F-pathway)''']] avoiding to overestimate it?'''<br />
Did you know that '''malate alone might support mt-respiration''' in [[Mitochondrial preparations| mitochondrial preparations]], if '''malate anaplerotic pathways''' ([[Malic enzyme|e.g. '''mt-malic enzyme''']]) are present? <br><br />
- Learn more about - »'''[[Malate-anaplerotic pathway control state|malate-anaplerotic pathway control state]]'''« <br><br />
- '''DatLab 7.4''' provides a step-by-step guide through the '''SUIT-027''' to evaluate the malate anaplerotic pathway - »'''[[SUIT-027 |SUIT-027]]'''« <br><br />
- Since [[Fatty acid oxidation pathway control state|F-pathway]] can be overestimated due to anaplerotic reactions, '''SUIT-002''' was specially designed to give information on '''F-pathway in OXPHOS state avoiding FAO overestimation''' in the presence of [[Anaplerosis|anaplerotic pathways]] - »'''[[SUIT-002 |SUIT-002]]'''«<br />
|}<br />
=== Apr 2020 ===<br />
<br />
* '''Apr-24 Fr''' <br />
[[File:ETC-.JPG|right|150px|Electron transport chain]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| ETC - the electron transport chain - is the most frequently applied misnomer in bioenergetics. Y Hatefi in the team of DE Green clarified this 58 years ago, introducing the concept of the [[Electron transfer pathway |electron transfer system]]. <br><br />
- »'''[[Hatefi 1962 J Biol Chem-XLII]]'''«<br />
|}<br />
<br><br />
* '''Apr-21 Tu''' <br />
[[File:CS.png|right|100px|Citrate synthase]]<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| [[Citrate synthase]] activity is commonly applied as a mitochondrial marker - and erroneous applications are common in the literature. <br><br />
- »'''[[MiPNet17.04 CitrateSynthase]]'''«<br />
|}<br />
<br><br />
* '''Apr-13 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br><br />
- »'''[[SUIT-024 |SUIT-024]]'''«<br />
|}<br />
<br><br />
* '''Apr-08 We'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage.''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum [[digitonin]] concentration. <br><br />
- »'''[[SUIT-010 |SUIT-010]]'''«<br />
|}<br />
<br><br />
* '''Apr-02 Th''' <br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] <br />
|'''Do you want to search the best [[SUIT]] protocol for your research questions?''' <br><br />
- Use the SUITbrowser: »'''[[SUITbrowser]]'''«<br />
|}<br />
=== Mar 2020 ===<br />
<br />
* '''Mar-16 Mo'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''The standardized coupling control protocol to explore the bioenergetics of living cells''' <br><br />
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions. <br><br />
- »'''[[SUIT-003 |SUIT-003]]'''«<br />
|}<br />
<br><br />
* '''Mar-11 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Does blebbistatin reduce oxygen dependence of respiration of [[permeabilized muscle fibers]]? This is one of the questions addressed in the COST Action CA15203 [[MitoEAGLE]] [[Doerrier_2019_MitoFit_Preprint_Arch_EA|WG2]]. <br><br />
- »'''[[Blebbistatin]]'''«<br />
|}<br />
<br><br />
* '''Mar-06 Fr'''<br />
:{|<br />
| width="100" align="center" | [[Image:SUIT icon.jpg|80px|link=https://wiki.oroboros.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]]<br />
| '''Explore metabolic profiles and specific modulations of mitochondrial pathways''' in health and disease with SUIT reference protocols. <br><br />
- »'''[[SUIT-001 |RP1]]« and »[[SUIT-002 |RP2]]'''«<br />
|}<br />
<br><br />
* '''Mar-03 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Rapamycin - more differences or more in common with other [[MitObesity drugs |mitObesity drugs]]? <br><br />
- »'''[[Rapamycin]]'''«<br />
|}<br />
<br />
=== Feb 2020 ===<br />
<br />
* '''Feb-26 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| It is essential to [[Uncoupler titrations |titrate uncouplers]] in multiple steps for respirometric measurement of [[ET capacity]]. <br><br />
- »'''[[Noncoupled respiration]]'''«<br />
|}<br />
<br><br />
* '''Feb-24 Mo'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| More research is needed on the impact of viral infection on mitochondrial function. <br><br />
- »'''[[Viruses and mitochondrial medicine]]'''«<br />
|}<br />
<br><br />
* '''Feb-19 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. <br><br />
- »'''[[Spermidine]]'''«<br />
|}<br />
<br><br />
* '''Feb-18 Tu'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Why do we fight gas bubbles? <br><br />
- Dive deep into »'''[[Oxygen solubility]]'''«<br />
|}<br />
<br><br />
* '''Feb-12 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| What do metformin, melatonin and other bioactive mitObesity compounds have in common? <br><br />
- »'''[[MitObesity drugs]]'''«<br />
|}<br />
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=== Jan 2020 ===<br />
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* '''Jan-16 Th'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Oxygen flux]]''' <br><br />
- which SI units should be used?<br />
|}<br />
<br><br />
<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| Did you ever think that units for body mass could be an issue? <br><br />
- »'''[[Body mass]]'''«<br />
|}<br />
<br><br />
* '''Jan-08 We'''<br />
:{|<br />
| width="100" align="center" | [[File:MitoPedia.jpg|80px|MitoPedia]]<br />
| '''[[Force]]''' and '''[[Energy]]''': this we all need in the New Year - and '''MitoPedia''' may help to clarify the difference.<br />
|}<br />
</div><br />
</div></div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Current_week_ecosystem_agenda&diff=205765
Template:Current week ecosystem agenda
2020-08-03T06:30:29Z
<p>Antunes Diana: </p>
<hr />
<div> 2020 Week 32<br />
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* '''Aug-03 Mo''' <br />
:{|<br />
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]<br />
| '''What do you do if the values for the “O2 slope background” fields are not appearing correctly in the DatLab “Mark statistics” window?'''<br><br />
It is possible to retrieve the values and correct units displayed in the Mark Statistics window by updating the Windows control panel settings. <br><br />
- » [[MitoPedia:_DatLab#Problems_with_text_in_the_.E2.80.9CMark_Statistics.E2.80.9D_window| Troubleshooting for Mark Statistics window]]«<br />
|}<br />
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* '''Aug-04 Tu'''<br />
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:{|<br />
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]<br />
|''' Liver-specific deletion of PARKIN impairs the mitochondrial phosphorylation system '''<br><br />
:from the O2k-Network »[[US PA Pittsburgh Jurczak MJ]]« <br />
*Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. [[Edmunds 2020 Mol Metab|»Bioblast link«]]<br />
|}<br />
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* '''Aug-05 We'''<br />
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* '''Aug-06 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| '''OXPHOS-coupling efficiency (1-[[LEAK_respiration|''L'']]/[[OXPHOS_capacity|''P'']])''' describes the coupling of the mitochondrial [[electron transfer system]]. This [[Coupling control factor|coupling control factor]] ranges from 1.0 for a fully coupled system to 0.0 for a system with zero respiratory phosphorylation capacity (''L''=''P''). It is suggested to use OXPHOS-coupling efficiency instead of the [[respiratory acceptor control ratio]] (''RCR'') to evaluate mitochondrial coupling. <br><br />
- »'''[[OXPHOS-coupling_efficiency|OXPHOS-coupling efficiency]]'''«<br />
|} <br />
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* '''Aug-07 Fr'''</div>
Antunes Diana
https://wiki.oroboros.at/index.php?title=Template:Next_oroboros_ecosystem_agenda&diff=205764
Template:Next oroboros ecosystem agenda
2020-08-03T06:29:58Z
<p>Antunes Diana: </p>
<hr />
<div><br />
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2020 Week 33<br />
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* '''Aug-10 Mo''' <br />
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* '''Aug-11 Tu'''<br />
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* '''Aug-12 We'''<br />
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* '''Aug-13 Th'''<br />
:{|<br />
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]]<br />
| Photosynthesis efficiency is reduced by 25% in plants and algae due the metabolic process called photorespiration. <br><br />
- »'''[[Photorespiration]]'''«<br />
|}<br />
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* '''Aug-14 Fr'''<br />
:{|<br />
| width="100" align="center" | [[File:O2k-brief.png|45px|link=http://wiki.oroboros.at/index.php/O2k-brief|O2k-brief repository]]<br />
|''' Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis''' <br><br />
: from the O2k-Network »[[NZ Auckland Hickey AJ]]« <br><br />
* Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. [[Chakraborty 2016 Pancreatology|»Bioblast link«]]<br />
|}<br />
2020 Week 34<br />
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* '''Aug-17 Mo''' <br />
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2020 Week 35<br />
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* '''Aug-26 We''' <br />
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* '''Aug-27 Th''' <br />
::::* Notification to oral and poster presenters - »[[MitoEAGLE Summit Obergurgl 2020]]« (2020 Nov 04-07) and »[[MiPschool Obergurgl 2020]]« (2020 Nov 09-11)<br />
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* '''Aug-28 Fr'''<br />
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2020 Week 36<br />
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* '''Aug-31 Mo''' <br />
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* '''Sep-02 We'''<br />
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* '''Sep-03 Th'''<br />
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* '''Sep-04 Fr'''<br />
::::[[File:Bioenergetics Vienna 2020.jpg|200px |link=Bioenergetics_Vienna_2020#Programme]] '''<big>[[Bioenergetics Vienna 2020]]</big>'''<br />
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* '''Sep-05 Sa''' - [[Bioenergetics Vienna 2020]]<br />
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* '''Sep-06 So''' <br />
::::* Abstract submission deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
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2020 Week 37<br />
* '''Sep-07 Mo''' <br />
::::* 19:00 / 20:15 '''MEGA Bildungsshow''' - 1 Million für Österreichs Talente. MARX HALLE Wien, Karl-Farkas-Gasse 19, 1030 Wien. - »[https://www.puls4.com/4GAMECHANGERS 4GAMECHANGERS]«<br />
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* '''Sep-08 Tu'''<br />
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* '''Sep-09 We'''<br />
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* '''Sep-10 Th'''<br />
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:::: »<big>'''[[36th Congress Czech Nutrition Society 2020 Hradec Kralove CZ]]'''</big>« (Sep 10-12)<br />
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* '''Sep-11 Fr'''<br />
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2020 Week 38<br />
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* '''Sep-14 Mo''' <br />
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* '''Sep-17 Th'''<br />
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2020 Week 39<br />
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* '''Sep-21 Mo''' <br />
:::: [[File:Oegmbt.png|100px|link=https://wiki.oroboros.at/index.php/12th_%C3%96GMBT_Annual_Meeting_2020_Graz_AT|ÖGMBT Graz AT]] <big>'''[[12th ÖGMBT Annual Meeting 2020 Graz AT]]'''</big> Sep 21-23<br />
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* '''Sep-22 Tu''' - [[12th ÖGMBT Annual Meeting 2020 Graz AT]] <br />
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* '''Sep-23 We''' - [[12th ÖGMBT Annual Meeting 2020 Graz AT]] <br />
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* '''Sep-24 Th'''<br />
::::* 18:00 - Houska Awards 2020 Vienna - [https://www.bcholding.at/houskapreis-1 Houskapreis der B&C Privatstiftung - Verleihung des Houskapreises 2020 in Wien]<br />
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* '''Sep-25 Fr'''<br />
<br />
2020 Week 40<br />
<br />
* '''Sep-28 Mo''' <br />
:::: [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.02 IOC Schroecken AT |<big>'''IOC Schroecken AT'''</big>]]<br />
<br />
----<br />
* '''Sep-29 Tu''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
----<br />
* '''Sep-30 We''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
::::* '''Registration and late Abstract Deadline''' - »[[MitoEAGLE Summit Obergurgl 2020]]« and »[[MiPschool Obergurgl 2020]]«<br />
::::* Early registration deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
----<br />
* '''Oct-01 Th''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
----<br />
* '''Oct-02 Fr''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
----<br />
* '''Oct-03 Sa''' - [[MiPNet25.02 IOC Schroecken AT |IOC Schroecken AT]]<br />
<br />
2020 Week 41<br />
<br />
* '''Oct-05 Mo''' <br />
<br />
----<br />
* '''Oct-06 Tu'''<br />
<br />
----<br />
* '''Oct-07 We'''<br />
:::: [[Image:O2k-Network.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] Pre-Conference O2k-Workshop »<big>'''[[MiPNet25.11 IOC148 Riga LV]] </big>'''«<br />
<br />
::::[[File:EU-CARDIOPROTECTION.png|100px|EU-CARDIOPROTECTION|link=6th EU-Cardioprotection Meeting 2020 Riga LV]] »<big>'''[[6th EU-Cardioprotection Meeting 2020 Riga LV]]'''</big>« (Oct 7-9)<br />
<br />
----<br />
* '''Oct-08 Th''' - [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
<br />
----<br />
* '''Oct-09 Fr''' - [[6th EU-Cardioprotection Meeting 2020 Riga LV]]<br />
:::: »<big>'''[[Long Night of Research 2020 Innsbruck AT]]'''</big>«<br />
<br />
2020 Week 42<br />
<br />
* '''Oct-12 Mo''' <br />
::::[[File:FAT4BRAIN logo.png|100px|FAT4BRAIN|link=FAT4BRAIN School IOC147 Riga LV]] »<big>'''[[FAT4BRAIN School IOC147 Riga LV]]'''</big>« (Oct 7-9)<br />
----<br />
* '''Oct-13 Tu'''<br />
::::[[FAT4BRAIN School IOC147 Riga LV]]<br />
----<br />
* '''Oct-14 We'''<br />
<br />
----<br />
* '''Oct-15 Th'''<br />
<br />
----<br />
* '''Oct-16 Fr'''<br />
<br />
----<br />
* '''Oct-18 Su'''<br />
:::: »<big>'''[[ISCaM 2020 London UK]]'''</big>«<br />
<br />
2020 Week 43<br />
<br />
* '''Oct-19 Mo''' - [[ISCaM 2020 London UK]]<br />
<br />
----<br />
* '''Oct-20 Tu''' - [[ISCaM 2020 London UK]]<br />
<br />
----<br />
* '''Oct-21 We'''<br />
<br />
----<br />
* '''Oct-22 Th'''<br />
<br />
----<br />
* '''Oct-23 Fr'''<br />
<br />
2020 Week 44<br />
<br />
* '''Oct-26 Mo''' <br />
<br />
----<br />
* '''Oct-27 Tu'''<br />
<br />
----<br />
* '''Oct-28 We'''<br />
<br />
----<br />
* '''Oct-29 Th'''<br />
<br />
----<br />
* '''Oct-30 Fr'''<br />
<br />
2020 Week 45<br />
<br />
* '''Nov-02 Mo''' <br />
<br />
----<br />
* '''Nov-03 Tu'''<br />
<br />
----<br />
* '''Nov-04 We'''<br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] <big>'''[[MitoEAGLE Summit Obergurgl 2020]]'''</big><br />
----<br />
* '''Nov-05 Th''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
----<br />
* '''Nov-06 Fr''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
* '''Nov-07 Sa''' - [[MitoEAGLE Summit Obergurgl 2020]]<br />
<br />
2020 Week 46<br />
<br />
* '''Nov-09 Mo''' <br />
:::: [[Image:MITOEAGLE-logo.jpg|60px|link=http://www.mitoeagle.org/index.php/MitoEAGLE|COST Action MitoEAGLE]] <big>'''[[MiPschool Obergurgl 2020]]'''</big> including '''[[TRANSMIT School 2020]]'''<br />
----<br />
* '''Nov-10 Tu''' - [[MiPschool Obergurgl 2020]] including [[TRANSMIT School 2020]]<br />
<br />
----<br />
* '''Nov-11 We''' - [[MiPschool Obergurgl 2020]] including [[TRANSMIT School 2020]]<br />
<br />
----<br />
* '''Nov-12 Th'''<br />
<br />
----<br />
* '''Nov-13 Fr'''<br />
<br />
2020 Week 47<br />
<br />
* '''Nov-16 Mo''' <br />
<br />
----<br />
* '''Nov-17 Tu'''<br />
<br />
----<br />
* '''Nov-18 We''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
<br />
----<br />
* '''Nov-19 Th''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
<br />
----<br />
* '''Nov-20 Fr''' <br />
::::[[File:ASMRM LOGO.JPG|75px|link=https://wiki.oroboros.at/index.php/Asia_Society_for_Mitochondrial_Research_and_Medicine ASMRM]]'''[[ASMRM 2020 Singapore SG]]'''<br />
<br />
2020 Week 48<br />
<br />
* '''Nov-23 Mo''' <br />
<br />
----<br />
* '''Nov-24 Tu'''<br />
<br />
----<br />
* '''Nov-25 We'''<br />
::::» <big>'''[[2020Spring PaduaMuscleDays Padova IT]]'''</big> / [[Image:O2k-Workshops.png|70px|O2k-Workshops|link=O2k-Events#Next_O2k-Workshops]] [[MiPNet25.04 IOC Padua IT |<big>'''IOC Padua IT'''</big>]] - ''separate registration''<br />
----<br />
* '''Nov-26 Th''' <br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
----<br />
* '''Nov-27 Fr'''<br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
----<br />
* '''Nov-28 Sa'''<br />
:::: [[2020Spring PaduaMuscleDays Padova IT]]<br />
<br />
2020 Week 49<br />
<br />
* '''Nov-30 Mo''' <br />
<br />
----<br />
* '''Dec-01 Tu'''<br />
:::: [[Image:AlgaeEurope.jpg|60px|AlgaeEurope]] <big>'''[[AlgaEurope 2020 Rome IT]]'''</big><br />
<br />
----<br />
* '''Dec-02 We'''<br />
<br />
----<br />
* '''Dec-03 Th'''<br />
<br />
----<br />
* '''Dec-04 Fr'''<br />
<br />
----<br />
* '''Dec-05 Sa'''<br />
<br />
----<br />
* '''Dec-06 So'''<br />
::::* Registration deadline - »[[ESSR 2020 Innsbruck AT]]«<br />
----<br />
2020 Week 50<br />
<br />
* '''Dec-07 Mo''' <br />
<br />
----<br />
* '''Dec-08 Tu'''<br />
<br />
----<br />
* '''Dec-09 We'''<br />
::::[[File:ESSR2020.png|100px|ESSR2020|link=https://wiki.oroboros.at/index.php/ESSR_2020_Innsbruck_AT]] <big>'''[[ESSR 2020 Innsbruck AT]]'''</big> (Dec 9-11)<br />
<br />
----<br />
* '''Dec-10 Th'''<br />
<br />
----<br />
* '''Dec-11 Fr'''<br />
<br />
2020 Week 51<br />
<br />
* '''Dec-14 Mo''' <br />
<br />
----<br />
* '''Dec-15 Tu'''<br />
<br />
----<br />
* '''Dec-16 We'''<br />
<br />
----<br />
* '''Dec-17 Th'''<br />
<br />
----<br />
* '''Dec-18 Fr'''<br />
<br />
2020 Week 52<br />
<br />
* '''Dec-21 Mo''' <br />
<br />
----<br />
* '''Dec-22 Tu'''<br />
<br />
----<br />
* '''Dec-23 We'''<br />
<br />
----<br />
* '''Dec-24 Th'''<br />
<br />
----<br />
* '''Dec-25 Fr'''<br />
<br />
2020 Week 53<br />
<br />
* '''Dec-28 Mo''' <br />
<br />
----<br />
* '''Dec-29 Tu'''<br />
<br />
----<br />
* '''Dec-30 We'''<br />
<br />
----<br />
* '''Dec-31 Th'''<br />
<br />
----<br />
* '''Jan-01 Fr'''</div>
Antunes Diana