Coupling-control state

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Coupling-control state


Coupling-control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ET states, with corresponding respiration rates (L, P, E) in any electron-transfer-pathway state which is competent for electron transfer. These coupling states are induced by titration of ADP and uncouplers, and application of specific inhibitors of the phosphorylation pathway. In living cells, the coupling-control states are LEAK, ROUTINE, and ET states of respiration with corresponding rates L, R, E, using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCCP). Coupling-control protocols induce these coupling control states sequentially at a constant electron-transfer-pathway state.

Abbreviation: CCS

Reference: BEC 2020.1, Gnaiger 2009 Int J Biochem Cell Biol

Coupling-control states: OXPHOS ROUTINE ET capacity LEAK - ROX

Communicated by Gnaiger E 2011-02-25, edited 2019-03-08.

SUITbrowser question: Coupling control

Coupling-control protocols, besides other SUIT protocols, allow to analyze coupling control in living cells or mitochondrial preparations.
Use the SUITbrowser to find the best protocol to answer this and other research questions.



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MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, SUIT state, Recommended 

MitoPedia methods: Respirometry 

MitoPedia topics: EAGLE