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Cruz 2018 Lipids Health Dis

From Bioblast
Publications in the MiPMap
Cruz MM, Lopes AB, Crisma AR, de Sรก RCC, Kuwabara WMT, Curi R, de Andrade PBM, Alonso-Vale MIC (2018) Palmitoleic acid (16:1n7) increases oxygen consumption, fatty acid oxidation and ATP content in white adipocytes. Lipids Health Dis 17:55.

ยป PMID: 29554895 Open Access

Cruz MM, Lopes AB, Crisma AR, de Sa RCC, Kuwabara WMT, Curi R, de Andrade PBM, Alonso-Vale MIC (2018) Lipids Health Dis

Abstract: We have recently demonstrated that palmitoleic acid (16:1n7) increases lipolysis, glucose uptake and glucose utilization for energy production in white adipose cells. In the present study, we tested the hypothesis that palmitoleic acid modulates bioenergetic activity in white adipocytes.

For this, 3 T3-L1 pre-adipocytes were differentiated into mature adipocytes in the presence (or absence) of palmitic (16:0) or palmitoleic (16:1n7) acid at 100 or 200 ฮผM. The following parameters were evaluated: lipolysis, lipogenesis, fatty acid (FA) oxidation, ATP content, oxygen consumption, mitochondrial mass, citrate synthase activity and protein content of mitochondrial oxidative phosphorylation (OXPHOS) complexes.

Treatment with 16:1n7 during 9 days raised basal and isoproterenol-stimulated lipolysis, FA incorporation into triacylglycerol (TAG), FA oxidation, oxygen consumption, protein expression of subunits representing OXPHOS complex II, III, and V and intracellular ATP content. These effects were not observed in adipocytes treated with 16:0.

Palmitoleic acid, by concerted action on lipolysis, FA esterification, mitochondrial FA oxidation, oxygen consumption and ATP content, does enhance white adipocyte energy expenditure and may act as local hormone. โ€ข Keywords: Beta-oxidation, Bioenergetics, Lipogenesis, Lipolysis, Mitochondria, Triglyceride/fatty acid cycle โ€ข Bioblast editor: Kandolf G โ€ข O2k-Network Lab: BR Sao Paulo Bresciani Martins de Andrade P


Labels: MiParea: Respiration 


Organism: Mouse  Tissue;cell: Fat, Other cell lines  Preparation: Intact cells 


Coupling state: ET  Pathway:HRR: Oxygraph-2k 

Labels, 2018-04