Du 1999 Free Radic Biol Med
|Du G, Willet K, Mouithys-Mickalad A, Sluse-Goffart CM, Droy-Lefaix M-T, Sluse FE (1999) EGb 761 protects liver mitochondria against injury induced by in vitro anoxia/reoxygenation. Free Radic Biol Med 27:596-604.|
Abstract: The present study investigated the protective effects of Ginkgo biloba extract (EGb 761) on rat liver mitochondrial damage induced by in vitro anoxia/reoxygenation. Anoxia/reoxygenation was known to impair respiratory activities and mitochondrial oxidative phosphorylation efficiency. ADP/O (2.57 ± 0.11) decreased after anoxia/reoxygenation (1.75 ± 0.09, p < .01), as well as state 3 and uncoupled respiration (−20%, p < .01), but state 4 respiration increased (p < .01). EGb 761 (50–200 μg/ml) had no effect on mitochondrial functions before anoxia, but had a specific dose-dependent protective effect after anoxia/reoxygenation. When mitochondria were incubated with 200 μg/ml EGb 761, they showed an increase in ADP/O (2.09 ± 0.14, p < .05) and a decrease in state 4 respiration (−22%) after anoxia/reoxygenation. In EPR spin-trapping measurement, EGb 761 decreased the EPR signal of superoxide anion produced during reoxygenation. In conclusion, EGb 761 specially protects mitochondrial ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria.
• Keywords: Ginkgo biloba extract, Mitochondria, Oxidative phosphorylation, Anoxia/reoxygenation, Superoxide anion, EPR, Free radicals
• O2k-Network Lab: BE Liege Votion DM
Stress:Ischemia-reperfusion Organism: Rat Tissue;cell: Liver Preparation: Isolated mitochondria
Coupling state: OXPHOS, ET