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Oroboros O2k-Core (O2k-Series D - G)

From Bioblast


                  


O2k-Open Support

Oroboros O2k-Core (O2k-Series D - G)



Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Description

O2k-Core-Concept.jpg

Oroboros O2k-Core - Former Series (O2k-Series D - G) - the experimental system complete for basic high-resolution respirometry (HRR). The O2k-Core includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System and the O2k-Titration Set. The O2k-Core supports all add-on O2k-Modules of the O2k.

On-line display of oxygen flux (rate of respiration) is provided in addition to the conventional 'oxygraphic' plot of oxygen concentration over time. Highest signal stability minimizes the required amounts of biological sample, and provides the basis for resolution in the extreme low-oxygen range. Peltier temperature control provides a thermal stability at ±0.002 °C in the range of 4 °C to 47 °C at typical constant room temperature. Electronically controlled PVDF or PEEK stirrers are integrated in the two-chamber design of the O2k, and a barometric pressure transducer enables automatic oxygen calibrations implemented in the DatLab software.

The O2k is a sole source apparatus with no other instruments meeting its specifications.


Reference: Gnaiger_2008_POS


  • A PC is required (not included), connecting DatLab to the O2k via a USB port. For PC requirements, see here.
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O2k-Core manual and selected protocols

» Contents: O2k-Core Manual DatLab 6.pdf
ChapterViewSectionLast update
Gnaiger 2008 POSBioblast pdf
O2k-Protocols contents
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52.2008
Gnaiger 2020 BEC MitoPathwaysOpen Access pdf published online 2020-12-30

Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-00022020
MiPNet04.05 Titration-InjectionBioblast pdf
O2k-Protocols contents
From step titration to ramp injection: Uncoupling by FCCP with TIP.
1999-01
MiPNet07.08 User informationBioblast pdf » Versions
O2k-Manual
O2k-Manual: user information. PLEASE STUDY THIS MANUAL.
2017-11-03
MiPNet08.09 CellRespirationBioblast pdf  »Versions
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
2016-08-11
MiPNet08.12 IOC22Bioblast pdf
O2k-Protocols
NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report.
2011-12-11
MiPNet12.01 Suppl T-issueBioblast pdf »Versions
O2k-Protocols contents
MitoPathways at the Q-junction: mouse skeletal muscle fibers.
2018-11-18
MiPNet12.11 MitoRespirationBioblast pdf »Versions
O2k-Protocols
Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control.
2014-02-20
MiPNet17.03 Shredder vs FibersBioblast pdf »Versions
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
2015-06-18
MiPNet19.18 O2k-Series G Core manualBioblast pdf »Versions
O2k-Manual
O2k-Core manual contents.
2016-08-08
MiPNet19.18B POS-serviceBioblast pdf »Versions
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.
2021-06-23
MiPNet19.18C DatLab 6: GuideBioblast pdf »Versions
O2k-Manual
DatLab-guide through the menus.
2016-08-24
MiPNet19.18D O2k-Series G and DatLab 6: CalibrationBioblast pdf »Versions
O2k-Protocols
O2k-calibration by DatLab 6.
2016-08-24
MiPNet19.18E O2 flux analysis - DatLab 6Bioblast pdf »Versions
O2k-Manual
Oxygen flux analysis: DatLab real-time.
2016-08-08
MiPNet21.14 Reference sample HRRBioblast pdf »Versions
O2k-Protocols
Development of a reference sample for HRR.
2016-10-19
MiPNet21.16 DatLab 7 Innovations
About Oroboros
DatLab 7: innovations.
2019-09-23
MiPNet22.16 DatLab 7 Innovations: DL-ProtocolsBioblast pdf » Versions
O2k-Protocols
DatLab 7.4 Innovations: DL-Protocols
2019-09-13
MiPNet23.12 DL-Protocols helpBioblast pdf » Versions
O2k-Protocols
DatLab Protocols: Help DatLab 7.
2019-09-13
MiPNet24.06 Oxygen flux analysis - DatLab 7.4Bioblast pdf » Versions
O2k-Protocols
Oxygen flux analysis with DatLab 7.4.
2020-04-16
MiPNet24.08 Safranin Analysis TemplateBioblast pdf » Versions
O2k-Protocols
Excel template for safranin data analysis.
2020-05-13
MiPNet24.09 Data analysis of mt-membrane potentialBioblast pdf » Versions
O2k-Protocols
Data analysis of mt-membrane potential.
2021-09-16
MiPNet25.14 TPP Analysis TemplateBioblast pdf » [https:.pdf Versions]
O2k-Protocols
Excel template for TPP data analysis.
2020-##-##
MiPNet26.06 DatLab 7: GuideBioblast pdf »Versions
O2k-Manual
DatLab-guide through the menus.
2021-06-02
MiPNet28.10 SmartPOS-servicePDF.jpg » Versions
O2k-Manual
SmartPOS: Service
2023-09-29
Pesta 2012 Methods Mol BiolPMID: 22057559
Bioblast pdf
O2k-Protocols
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_32012
Oxygraph-2k-Concept.jpg