Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Rindler 2013 J Biol Chem

From Bioblast
Publications in the MiPMap
Rindler PM, Plafker SM, Szweda LI, Kinter M (2013) High dietary fat selectively increases catalase expression within cardiac mitochondria. J Biol Chem 288:1979-90.

Β» PMID: 23204527 Open Access

Rindler PM, Plafker SM, Szweda LI, Kinter M (2013) J Biol Chem

Abstract: Obesity is a predictor of diabetes and cardiovascular disease. One consequence of obesity is dyslipidemia characterized by high blood triglycerides. It has been proposed that oxidative stress, driven by utilization of lipids for energy, contributes to these diseases. The effects of oxidative stress are mitigated by an endogenous antioxidant enzyme network, but little is known about its response to high fat utilization. Our experiments used a multiplexed quantitative proteomics method to measure antioxidant enzyme expression in heart tissue in a mouse model of diet-induced obesity. This experiment showed a rapid and specific up-regulation of catalase protein, with subsequent assays showing increases in activity and mRNA. Catalase, traditionally considered a peroxisomal protein, was found to be present in cardiac mitochondria and significantly increased in content and activity during high fat feeding. These data, coupled with the fact that fatty acid oxidation enhances mitochondrial H2O2 production, suggest that a localized catalase increase is needed to consume excessive mitochondrial H2O2 produced by increased fat metabolism. To determine whether the catalase-specific response is a common feature of physiological conditions that increase blood triglycerides and fatty acid oxidation, we measured changes in antioxidant expression in fasted versus fed mice. Indeed, a similar specific catalase increase was observed in mice fasted for 24 h. Our findings suggest a fundamental metabolic process in which catalase expression is regulated to prevent damage while preserving an H2O2-mediated sensing of diet composition that appropriately adjusts insulin sensitivity in the short term as needed to prioritize lipid metabolism for complete utilization. β€’ Keywords: Antioxidants, Heart, Metabolism, Mitochondria, Obesity, Peroxisomes, Catalase, High Fat Diet, Quantitative Proteomics, Selected Reaction Monitoring


Labels: MiParea: Exercise physiology;nutrition;life style  Pathology: Diabetes, Obesity  Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Heart 


Regulation: Fatty acid