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...irometric performance provides a quality control of cells or mitochondrial preparations; (ii) overtitration of uncouplers, incomplete action of inhibitors, or non- The O2k-Fluorescence LED2-Module is a LED and filter based fluorometry add-on module to be used together with the O2k. Optical s

...?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor] == O2k-Mitochondrial preparations ==

...lied for measurement of Ca²⁺ concentration with [[mitochondrial preparations]]. This dye fluoresces when bound to Ca²⁺. When measuring mitoch ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc

'''Sample types,mitochondrial preparations and sample addition:''' :::* [[Mitochondrial preparations]]

...uals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes. [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the smart-fluo sensor]<br

...on, explaining its toxic effect. This study further highlights the need to filter these wavelengths from the eyes of AMD patients. |preparations=Permeabilized cells

...by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mas |preparations=Intact cells

...each other by purification on ion exchange resins or by chromatography on filter paper. ...reduction in absorption at 455 nm when succinate was added to heart muscle preparations, and Axelrod et al. (3) noted a relationship between riboflavin deficiency

|preparations=Permeabilized tissue ...le from the filter paper and touch it once more shortly onto a dry area of filter paper while holding it with the forceps. Then immediately place the sample

...hodamine 123 can be used to measure mt-membrane potential in mitochondrial preparations. <br> ...ochondrial'' reactions contribute to ''residual oxygen consumption'' in mt-preparations and living cells. <br>

...ron flow from Complex II to Complex I can be investigated in mitochondrial preparations by measuring hydrogen peroxide production using [[Amplex UltraRed]] assay?' ...nomenon results in high hydrogen peroxide production in some mitochondrial preparations. The SUIT-026 protocol was designed to investigate this effect. <br>

...and is used for measuring mitochondrial ATP production by [[mitochondrial preparations]]. Determination of mitochondrial ATP production is based on the different ...] or [[O2k-Fluo LED2-Module]] selecting the [[Fluorescence-Sensor Blue]]/[[Filter Set MgG / CaG]]. Please see these pages for specific aspects of fluorescenc

::::» [[MitoPedia: Sample preparations]] ::::* Towards and beyond a mitochondrial physiology database: filter and amplify more signals from this data, making them more meaningful and va

...nt of [[hydrogen peroxide]] production ([[ROS]]) by cells or mitochondrial preparations. The reaction of H₂O₂ and AmR is catalyzed by [[horse ...the experimental AmR concentration on respiratory activity of cells or mt-preparations, by titration of AmR after a specific respiratory state has been reached in

Incorrect placement of TIP2k Filter Papers can lead to liquid accumulation and convective oxygen backdiffusion. ...tate is now distinguished from ROX state: REN is measured in mitochondrial preparations without addition of substrates, whereas ROX is due to oxidative side reacti

...rs underscores the importance of studying mitochondrial function in tissue preparations where mitochondrial structure is preserved and all mitochondria are represe ...p> necessary to trigger opening of the mPTP was 42 % lower in the isolated preparations (Figure 2E), demonstrating a marked sensitization of the mPTP to a Ca<sup>2

...hodamine 123 can be used to measure mt-membrane potential in mitochondrial preparations. <br> ...ron flow from Complex II to Complex I can be investigated in mitochondrial preparations by measuring hydrogen peroxide production using [[Amplex UltraRed]] assay?'

...ntent in cells or tissues, (''ii'') purification of isolated mitochondrial preparations, and (''iii'') determination of tissue mass or mt-markers. | '''Do you know how to select the correct fluorescence sensors and filter set?''' <br>

|'''Do I need to change the filter set of the O2k-Fluo Smart-Module?'''<br> Because two different filter sets can be used with the blue Fluo-Sensors, filters should be changed acco