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Talk:MiPMap

From Bioblast

Comments on MiPMap

  • Good concept regarding the MiPMap -- it could save lots of time and effort on the part of many investigators, not only by avoiding many hours of traditional search engine work, but also avoiding 're-inventing the wheel' in terms of experimental approaches. Hand SC (2012-12-26)

Example

In the MiPMap you would find information on mouse cell lines and tissues, e.g. mouse kidney mitochondria:

* Is malic enzyme present?
* Is the P/E<1.0 like in mouse brain but different from mouse skeletal muscle?
* Which UCPs are present?
* Preparation of isolated mitochondria, permeabilized tissue, homogenate.
* Which respiration medium is recommended? ->> Do not use media with high K+, which inhibits kidney mitochondrial respiration.

>> Is this a general issue for kidney, or is it in addition also a species issue? (according to Calzia E MiR05 works fine with kidney tissue from pig, but not in mouse).

>> Limitations of using MiR05 to MiR06Cr

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