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MitoPedia O2k and high-resolution respirometry: O2k-Open Support
- Question: Can we set the oxygen concentrations in the O2k to desired levels?
- Answer: Yes. - It is always possible to increase the oxygen concentration using the combination of catalase in the medium and injections of H2O2, as described in MiPNet14.3. By using the TIP2k, the oxygen concentration can be maintained between well defined limits, either using H2O2 or (for very low oxygen concentrations) air saturated medium in the TIP2k microsyringes. Such specification is called "oxystat" approach and supply appropriate templates for controlling the TIP2k. See also MiPNet12.10.
- Low oxygen levels can be reached depending on the application: (1) On a biological experiment this may be performed by injection of N2 in the gas phase, followed by closing the stoppers once the desired level of oxygen is reached. However, often the desired starting value is simply reached by waiting until the sample has consumed the required amount of oxygen, when the "oxystat" follows.
- For calibration and for instrumental O2 background tests purposes Dithionite is used to reduce oxygen levels, however this is not recommended in the presence of biological samples.